Synthesis and characterisation of mesoporous silicate nanoparticles for use as nanobiocatalysts

Autores
Bujedo, Agustín Anibal; Perillo, Maria Angelica; Burgos, Martha Ines
Año de publicación
2023
Idioma
inglés
Tipo de recurso
documento de conferencia
Estado
versión publicada
Descripción
Nano-biocatalysts are defined as those nanoparticles (NPs) that are capable of entrapping enzymes, transporting them and ultimately allowing catalysis to occur within the nanopores or releasing the enzyme in a controlled manner at the target site. NPs have the ability to interact with cells and tissues at the molecular level. It has been proposed that encapsulation in nanocarriers protects the bioactive components from unfavourable environmental conditions and also increases their solubility and bioavailability. We have synthesised mesoporous silicate nanoparticles using the method described by Nandiyanto et al1. It uses polystyrene as a template for the mesoporous structure and a surfactant and hydrocarbon solvent to control particle size. Different synthesis conditions were tested and the structural characteristics such as NP diameter and specific surface area (SSA) were analysed by SEM microscopy and dynamic vapour sorption, respectively. It was observed that NPs ranging from 52 to 98 nm were obtained and the SSA is compatible with mesoporous materials (194 m2 /g). Adsorption of the enzyme b-galactosidase from Kluyveromyces lactis was achieved at 48%. The chemical composition was analysed by EDS and mainly SO2 materials were observed.
Fil: Bujedo, Agustín Anibal. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas, Físicas y Naturales. Departamento de Química. Cátedra de Química Biológica; Argentina
Fil: Perillo, Maria Angelica. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones Biológicas y Tecnológicas. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas, Físicas y Naturales. Instituto de Investigaciones Biológicas y Tecnológicas; Argentina
Fil: Burgos, Martha Ines. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones Biológicas y Tecnológicas. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas, Físicas y Naturales. Instituto de Investigaciones Biológicas y Tecnológicas; Argentina
LI Reunión Anual de la Sociedad Argentina de Biofísica
Córdoba
Argentina
Sociedad Argentina de Biofísica
Materia
Mesoporous silica
Nanoparticles
Nanobiocatalyst
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/257086
Acceder
id CONICETDig_49be7e7f730fc34ed3916092d6fefa92
oai_identifier_str oai:ri.conicet.gov.ar:11336/257086
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Synthesis and characterisation of mesoporous silicate nanoparticles for use as nanobiocatalystsBujedo, Agustín AnibalPerillo, Maria AngelicaBurgos, Martha InesMesoporous silicaNanoparticlesNanobiocatalysthttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Nano-biocatalysts are defined as those nanoparticles (NPs) that are capable of entrapping enzymes, transporting them and ultimately allowing catalysis to occur within the nanopores or releasing the enzyme in a controlled manner at the target site. NPs have the ability to interact with cells and tissues at the molecular level. It has been proposed that encapsulation in nanocarriers protects the bioactive components from unfavourable environmental conditions and also increases their solubility and bioavailability. We have synthesised mesoporous silicate nanoparticles using the method described by Nandiyanto et al1. It uses polystyrene as a template for the mesoporous structure and a surfactant and hydrocarbon solvent to control particle size. Different synthesis conditions were tested and the structural characteristics such as NP diameter and specific surface area (SSA) were analysed by SEM microscopy and dynamic vapour sorption, respectively. It was observed that NPs ranging from 52 to 98 nm were obtained and the SSA is compatible with mesoporous materials (194 m2 /g). Adsorption of the enzyme b-galactosidase from Kluyveromyces lactis was achieved at 48%. The chemical composition was analysed by EDS and mainly SO2 materials were observed.Fil: Bujedo, Agustín Anibal. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas, Físicas y Naturales. Departamento de Química. Cátedra de Química Biológica; ArgentinaFil: Perillo, Maria Angelica. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones Biológicas y Tecnológicas. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas, Físicas y Naturales. Instituto de Investigaciones Biológicas y Tecnológicas; ArgentinaFil: Burgos, Martha Ines. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones Biológicas y Tecnológicas. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas, Físicas y Naturales. Instituto de Investigaciones Biológicas y Tecnológicas; ArgentinaLI Reunión Anual de la Sociedad Argentina de BiofísicaCórdobaArgentinaSociedad Argentina de BiofísicaSociedad Argentina de BiofísicaAcierno, Juan PabloCelej, Maria SoledadVazquez, Diego Sebastian2023info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/conferenceObjectReuniónBookhttp://purl.org/coar/resource_type/c_5794info:ar-repo/semantics/documentoDeConferenciaapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/257086Synthesis and characterisation of mesoporous silicate nanoparticles for use as nanobiocatalysts; LI Reunión Anual de la Sociedad Argentina de Biofísica; Córdoba; Argentina; 2023; 76-76978-987-48938-1-9CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://biofisica.org.ar/reuniones-cientificas/reunionsab-previas/Nacionalinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T09:41:11Zoai:ri.conicet.gov.ar:11336/257086instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 09:41:12.181CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Synthesis and characterisation of mesoporous silicate nanoparticles for use as nanobiocatalysts
title Synthesis and characterisation of mesoporous silicate nanoparticles for use as nanobiocatalysts
spellingShingle Synthesis and characterisation of mesoporous silicate nanoparticles for use as nanobiocatalysts
Bujedo, Agustín Anibal
Mesoporous silica
Nanoparticles
Nanobiocatalyst
title_short Synthesis and characterisation of mesoporous silicate nanoparticles for use as nanobiocatalysts
title_full Synthesis and characterisation of mesoporous silicate nanoparticles for use as nanobiocatalysts
title_fullStr Synthesis and characterisation of mesoporous silicate nanoparticles for use as nanobiocatalysts
title_full_unstemmed Synthesis and characterisation of mesoporous silicate nanoparticles for use as nanobiocatalysts
title_sort Synthesis and characterisation of mesoporous silicate nanoparticles for use as nanobiocatalysts
dc.creator.none.fl_str_mv Bujedo, Agustín Anibal
Perillo, Maria Angelica
Burgos, Martha Ines
author Bujedo, Agustín Anibal
author_facet Bujedo, Agustín Anibal
Perillo, Maria Angelica
Burgos, Martha Ines
author_role author
author2 Perillo, Maria Angelica
Burgos, Martha Ines
author2_role author
author
dc.contributor.none.fl_str_mv Acierno, Juan Pablo
Celej, Maria Soledad
Vazquez, Diego Sebastian
dc.subject.none.fl_str_mv Mesoporous silica
Nanoparticles
Nanobiocatalyst
topic Mesoporous silica
Nanoparticles
Nanobiocatalyst
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Nano-biocatalysts are defined as those nanoparticles (NPs) that are capable of entrapping enzymes, transporting them and ultimately allowing catalysis to occur within the nanopores or releasing the enzyme in a controlled manner at the target site. NPs have the ability to interact with cells and tissues at the molecular level. It has been proposed that encapsulation in nanocarriers protects the bioactive components from unfavourable environmental conditions and also increases their solubility and bioavailability. We have synthesised mesoporous silicate nanoparticles using the method described by Nandiyanto et al1. It uses polystyrene as a template for the mesoporous structure and a surfactant and hydrocarbon solvent to control particle size. Different synthesis conditions were tested and the structural characteristics such as NP diameter and specific surface area (SSA) were analysed by SEM microscopy and dynamic vapour sorption, respectively. It was observed that NPs ranging from 52 to 98 nm were obtained and the SSA is compatible with mesoporous materials (194 m2 /g). Adsorption of the enzyme b-galactosidase from Kluyveromyces lactis was achieved at 48%. The chemical composition was analysed by EDS and mainly SO2 materials were observed.
Fil: Bujedo, Agustín Anibal. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas, Físicas y Naturales. Departamento de Química. Cátedra de Química Biológica; Argentina
Fil: Perillo, Maria Angelica. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones Biológicas y Tecnológicas. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas, Físicas y Naturales. Instituto de Investigaciones Biológicas y Tecnológicas; Argentina
Fil: Burgos, Martha Ines. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones Biológicas y Tecnológicas. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas, Físicas y Naturales. Instituto de Investigaciones Biológicas y Tecnológicas; Argentina
LI Reunión Anual de la Sociedad Argentina de Biofísica
Córdoba
Argentina
Sociedad Argentina de Biofísica
description Nano-biocatalysts are defined as those nanoparticles (NPs) that are capable of entrapping enzymes, transporting them and ultimately allowing catalysis to occur within the nanopores or releasing the enzyme in a controlled manner at the target site. NPs have the ability to interact with cells and tissues at the molecular level. It has been proposed that encapsulation in nanocarriers protects the bioactive components from unfavourable environmental conditions and also increases their solubility and bioavailability. We have synthesised mesoporous silicate nanoparticles using the method described by Nandiyanto et al1. It uses polystyrene as a template for the mesoporous structure and a surfactant and hydrocarbon solvent to control particle size. Different synthesis conditions were tested and the structural characteristics such as NP diameter and specific surface area (SSA) were analysed by SEM microscopy and dynamic vapour sorption, respectively. It was observed that NPs ranging from 52 to 98 nm were obtained and the SSA is compatible with mesoporous materials (194 m2 /g). Adsorption of the enzyme b-galactosidase from Kluyveromyces lactis was achieved at 48%. The chemical composition was analysed by EDS and mainly SO2 materials were observed.
publishDate 2023
dc.date.none.fl_str_mv 2023
dc.type.none.fl_str_mv info:eu-repo/semantics/publishedVersion
info:eu-repo/semantics/conferenceObject
Reunión
Book
http://purl.org/coar/resource_type/c_5794
info:ar-repo/semantics/documentoDeConferencia
status_str publishedVersion
format conferenceObject
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/257086
Synthesis and characterisation of mesoporous silicate nanoparticles for use as nanobiocatalysts; LI Reunión Anual de la Sociedad Argentina de Biofísica; Córdoba; Argentina; 2023; 76-76
978-987-48938-1-9
CONICET Digital
CONICET
url http://hdl.handle.net/11336/257086
identifier_str_mv Synthesis and characterisation of mesoporous silicate nanoparticles for use as nanobiocatalysts; LI Reunión Anual de la Sociedad Argentina de Biofísica; Córdoba; Argentina; 2023; 76-76
978-987-48938-1-9
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://biofisica.org.ar/reuniones-cientificas/reunionsab-previas/
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.coverage.none.fl_str_mv Nacional
dc.publisher.none.fl_str_mv Sociedad Argentina de Biofísica
publisher.none.fl_str_mv Sociedad Argentina de Biofísica
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.069144

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