Functionalization of bamboo-like carbon nanotubes with 3-mercaptophenylboronic acid-modified gold nanoparticles for the development of a hybrid glucose enzyme electrochemical biose...

Autores
Eguílaz Rubio, Marcos; Villalonga, Reynaldo; Pingarrón, José M.; Ferreyra, Nancy Fabiana; Rivas, Gustavo Adolfo
Año de publicación
2015
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
We report for the first time a sensitive and selective glucose biosensor based on the immobilization at glassy carbon electrodes (GCE) of a new hybrid nanomaterial consisting of gold nanoparticles functionalized with 3-mercaptophenyl boronic acid (AuNPs-B(OH)2) and bamboo-like multiwall carbon nanotubes (bMWCNT) dispersed in hyperbranched polyethyleneimine (PEI). The presence of boronic acid residues allowed the supramolecular immobilization of glucose oxidase (GOx) as a model glycoenzyme while the hybrid nanomaterial Au(NP)-bMWCNT produces a synergistic effect on the catalytic detection of the enzymatically generated hydrogen peroxide. The resulting functionalized nanomaterials were characterized by TEM, FT-IR and electrochemical techniques. The sensitivity at 0.700 V was (3.26 ± 0.03) mA M−1 (28.6 mA M−1 cm−2), with a linear range between 2.50 × 10−4 M and 5.00 × 10−3 M, a detection limit of 0.8 μM and a quantification limit of 2.4 μM. The biocatalytic layer demonstrated to be highly reproducible with R.S.D. values of 8.6% for 10 successive amperometric calibrations using the same surface, and 4.5% for ten different bioelectrodes. The sensitivity of the biosensor after 14 days of storage at 4 °C remained at 86.1% of its original value. The combination of the excellent dispersing properties of PEI, the stability of the bMWCNT-PEI dispersion, the synergistic effect of AuNPs-B(OH)2 and bMWCNT towards the electrooxidation of hydrogen peroxide, and the robust immobilization of GOx at AuNP-B(OH)2 allowed building a sensitive, reproducible and stable amperometric biosensor for the quantification of glucose in beverages and milk samples.
Fil: Eguílaz Rubio, Marcos. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones en Físico-química de Córdoba. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Instituto de Investigaciones en Físico-química de Córdoba; Argentina
Fil: Villalonga, Reynaldo. Universidad Complutense de Madrid; España
Fil: Pingarrón, José M.. Universidad Complutense de Madrid; España
Fil: Ferreyra, Nancy Fabiana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones en Físico-química de Córdoba. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Instituto de Investigaciones en Físico-química de Córdoba; Argentina
Fil: Rivas, Gustavo Adolfo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones en Físico-química de Córdoba. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Instituto de Investigaciones en Físico-química de Córdoba; Argentina
Materia
Bamboo-Like Multiwalled Carbon Nanotubes
Carbon Nanotubes Dispersion
Polyethylenimine
Glucose Oxidase
Boronic Acid
Gold Nanoparticles
Amperometric Glucose Biosensor
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/44414
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/44414
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Functionalization of bamboo-like carbon nanotubes with 3-mercaptophenylboronic acid-modified gold nanoparticles for the development of a hybrid glucose enzyme electrochemical biosensorEguílaz Rubio, MarcosVillalonga, ReynaldoPingarrón, José M.Ferreyra, Nancy FabianaRivas, Gustavo AdolfoBamboo-Like Multiwalled Carbon NanotubesCarbon Nanotubes DispersionPolyethylenimineGlucose OxidaseBoronic AcidGold NanoparticlesAmperometric Glucose Biosensorhttps://purl.org/becyt/ford/1.4https://purl.org/becyt/ford/1We report for the first time a sensitive and selective glucose biosensor based on the immobilization at glassy carbon electrodes (GCE) of a new hybrid nanomaterial consisting of gold nanoparticles functionalized with 3-mercaptophenyl boronic acid (AuNPs-B(OH)2) and bamboo-like multiwall carbon nanotubes (bMWCNT) dispersed in hyperbranched polyethyleneimine (PEI). The presence of boronic acid residues allowed the supramolecular immobilization of glucose oxidase (GOx) as a model glycoenzyme while the hybrid nanomaterial Au(NP)-bMWCNT produces a synergistic effect on the catalytic detection of the enzymatically generated hydrogen peroxide. The resulting functionalized nanomaterials were characterized by TEM, FT-IR and electrochemical techniques. The sensitivity at 0.700 V was (3.26 ± 0.03) mA M−1 (28.6 mA M−1 cm−2), with a linear range between 2.50 × 10−4 M and 5.00 × 10−3 M, a detection limit of 0.8 μM and a quantification limit of 2.4 μM. The biocatalytic layer demonstrated to be highly reproducible with R.S.D. values of 8.6% for 10 successive amperometric calibrations using the same surface, and 4.5% for ten different bioelectrodes. The sensitivity of the biosensor after 14 days of storage at 4 °C remained at 86.1% of its original value. The combination of the excellent dispersing properties of PEI, the stability of the bMWCNT-PEI dispersion, the synergistic effect of AuNPs-B(OH)2 and bMWCNT towards the electrooxidation of hydrogen peroxide, and the robust immobilization of GOx at AuNP-B(OH)2 allowed building a sensitive, reproducible and stable amperometric biosensor for the quantification of glucose in beverages and milk samples.Fil: Eguílaz Rubio, Marcos. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones en Físico-química de Córdoba. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Instituto de Investigaciones en Físico-química de Córdoba; ArgentinaFil: Villalonga, Reynaldo. Universidad Complutense de Madrid; EspañaFil: Pingarrón, José M.. Universidad Complutense de Madrid; EspañaFil: Ferreyra, Nancy Fabiana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones en Físico-química de Córdoba. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Instituto de Investigaciones en Físico-química de Córdoba; ArgentinaFil: Rivas, Gustavo Adolfo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones en Físico-química de Córdoba. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Instituto de Investigaciones en Físico-química de Córdoba; ArgentinaElsevier Science Sa2015-04info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/44414Eguílaz Rubio, Marcos; Villalonga, Reynaldo; Pingarrón, José M.; Ferreyra, Nancy Fabiana; Rivas, Gustavo Adolfo; Functionalization of bamboo-like carbon nanotubes with 3-mercaptophenylboronic acid-modified gold nanoparticles for the development of a hybrid glucose enzyme electrochemical biosensor; Elsevier Science Sa; Sensors and Actuators B: Chemical; 216; 4-2015; 629-6370925-4005CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/info:eu-repo/semantics/altIdentifier/doi/10.1016/j.snb.2015.03.112info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-29T11:44:52Zoai:ri.conicet.gov.ar:11336/44414instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-29 11:44:53.225CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Functionalization of bamboo-like carbon nanotubes with 3-mercaptophenylboronic acid-modified gold nanoparticles for the development of a hybrid glucose enzyme electrochemical biosensor
title Functionalization of bamboo-like carbon nanotubes with 3-mercaptophenylboronic acid-modified gold nanoparticles for the development of a hybrid glucose enzyme electrochemical biosensor
spellingShingle Functionalization of bamboo-like carbon nanotubes with 3-mercaptophenylboronic acid-modified gold nanoparticles for the development of a hybrid glucose enzyme electrochemical biosensor
Eguílaz Rubio, Marcos
Bamboo-Like Multiwalled Carbon Nanotubes
Carbon Nanotubes Dispersion
Polyethylenimine
Glucose Oxidase
Boronic Acid
Gold Nanoparticles
Amperometric Glucose Biosensor
title_short Functionalization of bamboo-like carbon nanotubes with 3-mercaptophenylboronic acid-modified gold nanoparticles for the development of a hybrid glucose enzyme electrochemical biosensor
title_full Functionalization of bamboo-like carbon nanotubes with 3-mercaptophenylboronic acid-modified gold nanoparticles for the development of a hybrid glucose enzyme electrochemical biosensor
title_fullStr Functionalization of bamboo-like carbon nanotubes with 3-mercaptophenylboronic acid-modified gold nanoparticles for the development of a hybrid glucose enzyme electrochemical biosensor
title_full_unstemmed Functionalization of bamboo-like carbon nanotubes with 3-mercaptophenylboronic acid-modified gold nanoparticles for the development of a hybrid glucose enzyme electrochemical biosensor
title_sort Functionalization of bamboo-like carbon nanotubes with 3-mercaptophenylboronic acid-modified gold nanoparticles for the development of a hybrid glucose enzyme electrochemical biosensor
dc.creator.none.fl_str_mv Eguílaz Rubio, Marcos
Villalonga, Reynaldo
Pingarrón, José M.
Ferreyra, Nancy Fabiana
Rivas, Gustavo Adolfo
author Eguílaz Rubio, Marcos
author_facet Eguílaz Rubio, Marcos
Villalonga, Reynaldo
Pingarrón, José M.
Ferreyra, Nancy Fabiana
Rivas, Gustavo Adolfo
author_role author
author2 Villalonga, Reynaldo
Pingarrón, José M.
Ferreyra, Nancy Fabiana
Rivas, Gustavo Adolfo
author2_role author
author
author
author
dc.subject.none.fl_str_mv Bamboo-Like Multiwalled Carbon Nanotubes
Carbon Nanotubes Dispersion
Polyethylenimine
Glucose Oxidase
Boronic Acid
Gold Nanoparticles
Amperometric Glucose Biosensor
topic Bamboo-Like Multiwalled Carbon Nanotubes
Carbon Nanotubes Dispersion
Polyethylenimine
Glucose Oxidase
Boronic Acid
Gold Nanoparticles
Amperometric Glucose Biosensor
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.4
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv We report for the first time a sensitive and selective glucose biosensor based on the immobilization at glassy carbon electrodes (GCE) of a new hybrid nanomaterial consisting of gold nanoparticles functionalized with 3-mercaptophenyl boronic acid (AuNPs-B(OH)2) and bamboo-like multiwall carbon nanotubes (bMWCNT) dispersed in hyperbranched polyethyleneimine (PEI). The presence of boronic acid residues allowed the supramolecular immobilization of glucose oxidase (GOx) as a model glycoenzyme while the hybrid nanomaterial Au(NP)-bMWCNT produces a synergistic effect on the catalytic detection of the enzymatically generated hydrogen peroxide. The resulting functionalized nanomaterials were characterized by TEM, FT-IR and electrochemical techniques. The sensitivity at 0.700 V was (3.26 ± 0.03) mA M−1 (28.6 mA M−1 cm−2), with a linear range between 2.50 × 10−4 M and 5.00 × 10−3 M, a detection limit of 0.8 μM and a quantification limit of 2.4 μM. The biocatalytic layer demonstrated to be highly reproducible with R.S.D. values of 8.6% for 10 successive amperometric calibrations using the same surface, and 4.5% for ten different bioelectrodes. The sensitivity of the biosensor after 14 days of storage at 4 °C remained at 86.1% of its original value. The combination of the excellent dispersing properties of PEI, the stability of the bMWCNT-PEI dispersion, the synergistic effect of AuNPs-B(OH)2 and bMWCNT towards the electrooxidation of hydrogen peroxide, and the robust immobilization of GOx at AuNP-B(OH)2 allowed building a sensitive, reproducible and stable amperometric biosensor for the quantification of glucose in beverages and milk samples.
Fil: Eguílaz Rubio, Marcos. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones en Físico-química de Córdoba. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Instituto de Investigaciones en Físico-química de Córdoba; Argentina
Fil: Villalonga, Reynaldo. Universidad Complutense de Madrid; España
Fil: Pingarrón, José M.. Universidad Complutense de Madrid; España
Fil: Ferreyra, Nancy Fabiana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones en Físico-química de Córdoba. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Instituto de Investigaciones en Físico-química de Córdoba; Argentina
Fil: Rivas, Gustavo Adolfo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones en Físico-química de Córdoba. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Instituto de Investigaciones en Físico-química de Córdoba; Argentina
description We report for the first time a sensitive and selective glucose biosensor based on the immobilization at glassy carbon electrodes (GCE) of a new hybrid nanomaterial consisting of gold nanoparticles functionalized with 3-mercaptophenyl boronic acid (AuNPs-B(OH)2) and bamboo-like multiwall carbon nanotubes (bMWCNT) dispersed in hyperbranched polyethyleneimine (PEI). The presence of boronic acid residues allowed the supramolecular immobilization of glucose oxidase (GOx) as a model glycoenzyme while the hybrid nanomaterial Au(NP)-bMWCNT produces a synergistic effect on the catalytic detection of the enzymatically generated hydrogen peroxide. The resulting functionalized nanomaterials were characterized by TEM, FT-IR and electrochemical techniques. The sensitivity at 0.700 V was (3.26 ± 0.03) mA M−1 (28.6 mA M−1 cm−2), with a linear range between 2.50 × 10−4 M and 5.00 × 10−3 M, a detection limit of 0.8 μM and a quantification limit of 2.4 μM. The biocatalytic layer demonstrated to be highly reproducible with R.S.D. values of 8.6% for 10 successive amperometric calibrations using the same surface, and 4.5% for ten different bioelectrodes. The sensitivity of the biosensor after 14 days of storage at 4 °C remained at 86.1% of its original value. The combination of the excellent dispersing properties of PEI, the stability of the bMWCNT-PEI dispersion, the synergistic effect of AuNPs-B(OH)2 and bMWCNT towards the electrooxidation of hydrogen peroxide, and the robust immobilization of GOx at AuNP-B(OH)2 allowed building a sensitive, reproducible and stable amperometric biosensor for the quantification of glucose in beverages and milk samples.
publishDate 2015
dc.date.none.fl_str_mv 2015-04
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/44414
Eguílaz Rubio, Marcos; Villalonga, Reynaldo; Pingarrón, José M.; Ferreyra, Nancy Fabiana; Rivas, Gustavo Adolfo; Functionalization of bamboo-like carbon nanotubes with 3-mercaptophenylboronic acid-modified gold nanoparticles for the development of a hybrid glucose enzyme electrochemical biosensor; Elsevier Science Sa; Sensors and Actuators B: Chemical; 216; 4-2015; 629-637
0925-4005
CONICET Digital
CONICET
url http://hdl.handle.net/11336/44414
identifier_str_mv Eguílaz Rubio, Marcos; Villalonga, Reynaldo; Pingarrón, José M.; Ferreyra, Nancy Fabiana; Rivas, Gustavo Adolfo; Functionalization of bamboo-like carbon nanotubes with 3-mercaptophenylboronic acid-modified gold nanoparticles for the development of a hybrid glucose enzyme electrochemical biosensor; Elsevier Science Sa; Sensors and Actuators B: Chemical; 216; 4-2015; 629-637
0925-4005
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/
info:eu-repo/semantics/altIdentifier/doi/10.1016/j.snb.2015.03.112
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Elsevier Science Sa
publisher.none.fl_str_mv Elsevier Science Sa
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.10058

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