Bamboo-like multiwalled carbon nanotubes dispersed in double stranded calf-thymus DNA as a new analytical platform for building layer-by-layer based biosensors

Autores
Primo, Emiliano Nicolás; Gutierrez, Fabiana Andrea; Rubianes, María Dolores; Rivas, Gustavo Adolfo
Año de publicación
2015
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
This work reports the successful application of bamboo-like multiwalled carbon nanotubes (bCNT) non-covalently functionalized with calf-thymus double stranded DNA (dsDNA) as a robust platform (bCNT-dsDNA) to build electrochemical biosensors. The “model system” proposed here as a proof of concept was an enzymatic biosensor devoted to glucose quantification obtained by layer-by-layer self-assemby of polydiallyldimethylammonium (PDDA) and glucose oxidase (GOx) at glassy carbon electrodes (GCE) modified with bCNT-dsDNA (GCE/bCNT-dsDNA/(PDDA/GOx)n). The influence of GOx and PDDA assembling conditions and the effect of the number of PDDA/GOx bilayers (n) on the performance of the resulting biosensor is critically discussed. The supramolecular architecture was characterized by electrochemical impedance spectroscopy from the charge transfer resistance of quinone/hydroquinone and potassium ferrocyanide/potassium ferricyanide; by cyclic voltammetry from the surface concentration of GOx using ferrocene methanol as enzyme regenerator; by amperometry from the response of the enzymatically generated hydrogen peroxide; and by surface plasmon resonance from the changes in the plasmon resonance angle. The analytical parameters obtained with GCE/bCNT-dsDNA/(PDDA/GOx)3 for the amperometric quantification of glucose at 0.700 V were: sensitivity of (265 ± 7) μA mM−1 cm−2, linear range between 0.25 and 2.50 × 10−3 M, detection limit of 50 μM, repeatability of 3.6% (n = 10), and negligible interference from maltose, galactose, fructose and manose. The biosensor was successfully used for the sensitive quantification of glucose in beverages and a medicine sample.
Fil: Primo, Emiliano Nicolás. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones en Físico-química de Córdoba. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Instituto de Investigaciones en Físico-química de Córdoba; Argentina
Fil: Gutierrez, Fabiana Andrea. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones en Físico-química de Córdoba. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Instituto de Investigaciones en Físico-química de Córdoba; Argentina
Fil: Rubianes, María Dolores. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones en Físico-química de Córdoba. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Instituto de Investigaciones en Físico-química de Córdoba; Argentina
Fil: Rivas, Gustavo Adolfo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones en Físico-química de Córdoba. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Instituto de Investigaciones en Físico-química de Córdoba; Argentina
Materia
Carbon Nanotubes Functionalization
Calf-Thymus Double Stranded
Glucose Oxidase
Layer-By-Layer Biosensors
Self-Assembly
Electrochemical Glucose Biosensor
Bamboo-Like Multiwalled Carbon Nanotubes
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/47381
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/47381
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Bamboo-like multiwalled carbon nanotubes dispersed in double stranded calf-thymus DNA as a new analytical platform for building layer-by-layer based biosensorsPrimo, Emiliano NicolásGutierrez, Fabiana AndreaRubianes, María DoloresRivas, Gustavo AdolfoCarbon Nanotubes FunctionalizationCalf-Thymus Double StrandedGlucose OxidaseLayer-By-Layer BiosensorsSelf-AssemblyElectrochemical Glucose BiosensorBamboo-Like Multiwalled Carbon Nanotubeshttps://purl.org/becyt/ford/1.4https://purl.org/becyt/ford/1This work reports the successful application of bamboo-like multiwalled carbon nanotubes (bCNT) non-covalently functionalized with calf-thymus double stranded DNA (dsDNA) as a robust platform (bCNT-dsDNA) to build electrochemical biosensors. The “model system” proposed here as a proof of concept was an enzymatic biosensor devoted to glucose quantification obtained by layer-by-layer self-assemby of polydiallyldimethylammonium (PDDA) and glucose oxidase (GOx) at glassy carbon electrodes (GCE) modified with bCNT-dsDNA (GCE/bCNT-dsDNA/(PDDA/GOx)n). The influence of GOx and PDDA assembling conditions and the effect of the number of PDDA/GOx bilayers (n) on the performance of the resulting biosensor is critically discussed. The supramolecular architecture was characterized by electrochemical impedance spectroscopy from the charge transfer resistance of quinone/hydroquinone and potassium ferrocyanide/potassium ferricyanide; by cyclic voltammetry from the surface concentration of GOx using ferrocene methanol as enzyme regenerator; by amperometry from the response of the enzymatically generated hydrogen peroxide; and by surface plasmon resonance from the changes in the plasmon resonance angle. The analytical parameters obtained with GCE/bCNT-dsDNA/(PDDA/GOx)3 for the amperometric quantification of glucose at 0.700 V were: sensitivity of (265 ± 7) μA mM−1 cm−2, linear range between 0.25 and 2.50 × 10−3 M, detection limit of 50 μM, repeatability of 3.6% (n = 10), and negligible interference from maltose, galactose, fructose and manose. The biosensor was successfully used for the sensitive quantification of glucose in beverages and a medicine sample.Fil: Primo, Emiliano Nicolás. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones en Físico-química de Córdoba. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Instituto de Investigaciones en Físico-química de Córdoba; ArgentinaFil: Gutierrez, Fabiana Andrea. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones en Físico-química de Córdoba. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Instituto de Investigaciones en Físico-química de Córdoba; ArgentinaFil: Rubianes, María Dolores. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones en Físico-química de Córdoba. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Instituto de Investigaciones en Físico-química de Córdoba; ArgentinaFil: Rivas, Gustavo Adolfo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones en Físico-química de Córdoba. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Instituto de Investigaciones en Físico-química de Córdoba; ArgentinaPergamon-Elsevier Science Ltd2015-11-10info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/47381Primo, Emiliano Nicolás; Gutierrez, Fabiana Andrea; Rubianes, María Dolores; Rivas, Gustavo Adolfo; Bamboo-like multiwalled carbon nanotubes dispersed in double stranded calf-thymus DNA as a new analytical platform for building layer-by-layer based biosensors; Pergamon-Elsevier Science Ltd; Electrochimica Acta; 182; 10-11-2015; 391-3970013-4686CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/http://www.sciencedirect.com/science/article/pii/S0013468615304400info:eu-repo/semantics/altIdentifier/doi/10.1016/j.electacta.2015.09.028info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T09:33:18Zoai:ri.conicet.gov.ar:11336/47381instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 09:33:19.078CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Bamboo-like multiwalled carbon nanotubes dispersed in double stranded calf-thymus DNA as a new analytical platform for building layer-by-layer based biosensors
title Bamboo-like multiwalled carbon nanotubes dispersed in double stranded calf-thymus DNA as a new analytical platform for building layer-by-layer based biosensors
spellingShingle Bamboo-like multiwalled carbon nanotubes dispersed in double stranded calf-thymus DNA as a new analytical platform for building layer-by-layer based biosensors
Primo, Emiliano Nicolás
Carbon Nanotubes Functionalization
Calf-Thymus Double Stranded
Glucose Oxidase
Layer-By-Layer Biosensors
Self-Assembly
Electrochemical Glucose Biosensor
Bamboo-Like Multiwalled Carbon Nanotubes
title_short Bamboo-like multiwalled carbon nanotubes dispersed in double stranded calf-thymus DNA as a new analytical platform for building layer-by-layer based biosensors
title_full Bamboo-like multiwalled carbon nanotubes dispersed in double stranded calf-thymus DNA as a new analytical platform for building layer-by-layer based biosensors
title_fullStr Bamboo-like multiwalled carbon nanotubes dispersed in double stranded calf-thymus DNA as a new analytical platform for building layer-by-layer based biosensors
title_full_unstemmed Bamboo-like multiwalled carbon nanotubes dispersed in double stranded calf-thymus DNA as a new analytical platform for building layer-by-layer based biosensors
title_sort Bamboo-like multiwalled carbon nanotubes dispersed in double stranded calf-thymus DNA as a new analytical platform for building layer-by-layer based biosensors
dc.creator.none.fl_str_mv Primo, Emiliano Nicolás
Gutierrez, Fabiana Andrea
Rubianes, María Dolores
Rivas, Gustavo Adolfo
author Primo, Emiliano Nicolás
author_facet Primo, Emiliano Nicolás
Gutierrez, Fabiana Andrea
Rubianes, María Dolores
Rivas, Gustavo Adolfo
author_role author
author2 Gutierrez, Fabiana Andrea
Rubianes, María Dolores
Rivas, Gustavo Adolfo
author2_role author
author
author
dc.subject.none.fl_str_mv Carbon Nanotubes Functionalization
Calf-Thymus Double Stranded
Glucose Oxidase
Layer-By-Layer Biosensors
Self-Assembly
Electrochemical Glucose Biosensor
Bamboo-Like Multiwalled Carbon Nanotubes
topic Carbon Nanotubes Functionalization
Calf-Thymus Double Stranded
Glucose Oxidase
Layer-By-Layer Biosensors
Self-Assembly
Electrochemical Glucose Biosensor
Bamboo-Like Multiwalled Carbon Nanotubes
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.4
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv This work reports the successful application of bamboo-like multiwalled carbon nanotubes (bCNT) non-covalently functionalized with calf-thymus double stranded DNA (dsDNA) as a robust platform (bCNT-dsDNA) to build electrochemical biosensors. The “model system” proposed here as a proof of concept was an enzymatic biosensor devoted to glucose quantification obtained by layer-by-layer self-assemby of polydiallyldimethylammonium (PDDA) and glucose oxidase (GOx) at glassy carbon electrodes (GCE) modified with bCNT-dsDNA (GCE/bCNT-dsDNA/(PDDA/GOx)n). The influence of GOx and PDDA assembling conditions and the effect of the number of PDDA/GOx bilayers (n) on the performance of the resulting biosensor is critically discussed. The supramolecular architecture was characterized by electrochemical impedance spectroscopy from the charge transfer resistance of quinone/hydroquinone and potassium ferrocyanide/potassium ferricyanide; by cyclic voltammetry from the surface concentration of GOx using ferrocene methanol as enzyme regenerator; by amperometry from the response of the enzymatically generated hydrogen peroxide; and by surface plasmon resonance from the changes in the plasmon resonance angle. The analytical parameters obtained with GCE/bCNT-dsDNA/(PDDA/GOx)3 for the amperometric quantification of glucose at 0.700 V were: sensitivity of (265 ± 7) μA mM−1 cm−2, linear range between 0.25 and 2.50 × 10−3 M, detection limit of 50 μM, repeatability of 3.6% (n = 10), and negligible interference from maltose, galactose, fructose and manose. The biosensor was successfully used for the sensitive quantification of glucose in beverages and a medicine sample.
Fil: Primo, Emiliano Nicolás. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones en Físico-química de Córdoba. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Instituto de Investigaciones en Físico-química de Córdoba; Argentina
Fil: Gutierrez, Fabiana Andrea. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones en Físico-química de Córdoba. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Instituto de Investigaciones en Físico-química de Córdoba; Argentina
Fil: Rubianes, María Dolores. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones en Físico-química de Córdoba. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Instituto de Investigaciones en Físico-química de Córdoba; Argentina
Fil: Rivas, Gustavo Adolfo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones en Físico-química de Córdoba. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Instituto de Investigaciones en Físico-química de Córdoba; Argentina
description This work reports the successful application of bamboo-like multiwalled carbon nanotubes (bCNT) non-covalently functionalized with calf-thymus double stranded DNA (dsDNA) as a robust platform (bCNT-dsDNA) to build electrochemical biosensors. The “model system” proposed here as a proof of concept was an enzymatic biosensor devoted to glucose quantification obtained by layer-by-layer self-assemby of polydiallyldimethylammonium (PDDA) and glucose oxidase (GOx) at glassy carbon electrodes (GCE) modified with bCNT-dsDNA (GCE/bCNT-dsDNA/(PDDA/GOx)n). The influence of GOx and PDDA assembling conditions and the effect of the number of PDDA/GOx bilayers (n) on the performance of the resulting biosensor is critically discussed. The supramolecular architecture was characterized by electrochemical impedance spectroscopy from the charge transfer resistance of quinone/hydroquinone and potassium ferrocyanide/potassium ferricyanide; by cyclic voltammetry from the surface concentration of GOx using ferrocene methanol as enzyme regenerator; by amperometry from the response of the enzymatically generated hydrogen peroxide; and by surface plasmon resonance from the changes in the plasmon resonance angle. The analytical parameters obtained with GCE/bCNT-dsDNA/(PDDA/GOx)3 for the amperometric quantification of glucose at 0.700 V were: sensitivity of (265 ± 7) μA mM−1 cm−2, linear range between 0.25 and 2.50 × 10−3 M, detection limit of 50 μM, repeatability of 3.6% (n = 10), and negligible interference from maltose, galactose, fructose and manose. The biosensor was successfully used for the sensitive quantification of glucose in beverages and a medicine sample.
publishDate 2015
dc.date.none.fl_str_mv 2015-11-10
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/47381
Primo, Emiliano Nicolás; Gutierrez, Fabiana Andrea; Rubianes, María Dolores; Rivas, Gustavo Adolfo; Bamboo-like multiwalled carbon nanotubes dispersed in double stranded calf-thymus DNA as a new analytical platform for building layer-by-layer based biosensors; Pergamon-Elsevier Science Ltd; Electrochimica Acta; 182; 10-11-2015; 391-397
0013-4686
CONICET Digital
CONICET
url http://hdl.handle.net/11336/47381
identifier_str_mv Primo, Emiliano Nicolás; Gutierrez, Fabiana Andrea; Rubianes, María Dolores; Rivas, Gustavo Adolfo; Bamboo-like multiwalled carbon nanotubes dispersed in double stranded calf-thymus DNA as a new analytical platform for building layer-by-layer based biosensors; Pergamon-Elsevier Science Ltd; Electrochimica Acta; 182; 10-11-2015; 391-397
0013-4686
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/http://www.sciencedirect.com/science/article/pii/S0013468615304400
info:eu-repo/semantics/altIdentifier/doi/10.1016/j.electacta.2015.09.028
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Pergamon-Elsevier Science Ltd
publisher.none.fl_str_mv Pergamon-Elsevier Science Ltd
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.070432

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