Epichromatin is conserved in Toxoplasma gondii and labels the exterior parasite chromatin throughout the cell cycle

Autores
Vanagas, Laura; Dalmasso, Maria Carolina; Dubremetz, Jean F.; Portiansky, Enrique Leo; Olins, Donald E.; Angel, Sergio Oscar
Año de publicación
2013
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Toxoplasma gondii is an Apicomplexan intracellular protozoan parasite responsible for Toxoplasmosis, a disease with considerable medical and economic impact worldwide. T. gondii cells never lose the nuclear envelope and their chromosomes do not present a higher order level of condensation during mitosis. Here, we tested the murine monoclonal antibody PL2-6, which labels epichromatin, a conformational chromatin epitope based on DNA+H2As and H2Bs, in T. gondii cultured on fibroblast cells. This epitope is present on the exterior chromatin surface of interphase nuclei and on the periphery of metaphasic chromosomes in higher eukaryotes. PL2-6 reacted with T. gondii H2As and H2Bs histones in Western blot assays. Besides, the antibody reacted with the nuclear fraction of tachyzoites, as a single band coincident with H2B histone. In the T. gondii tachyzoite stage, PL2-6 also had peripheral nuclear localization, as observed by epifluorescence/confocal microscopy and also immunoelectron microscopy. Confocal analysis showed that epichromatin is slightly polarized to one face of the parasite exterior chromatin surface. In replicating tachyzoites, PL2-6 also labels the exterior chromatin surface, covering the face of both segregating nuclei, facing the plasma membrane of the mother cell. The possible role of epichromatin in T. gondii is discussed.
Fil: Vanagas, Laura. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Biotecnológicas. Instituto de Investigaciones Biotecnológicas "Dr. Raúl Alfonsín" (sede Chascomús). Universidad Nacional de San Martín. Instituto de Investigaciones Biotecnológicas. Instituto de Investigaciones Biotecnológicas "Dr. Raúl Alfonsín" (sede Chascomús); Argentina
Fil: Dalmasso, Maria Carolina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Biotecnológicas. Instituto de Investigaciones Biotecnológicas "Dr. Raúl Alfonsín" (sede Chascomús). Universidad Nacional de San Martín. Instituto de Investigaciones Biotecnológicas. Instituto de Investigaciones Biotecnológicas "Dr. Raúl Alfonsín" (sede Chascomús); Argentina
Fil: Dubremetz, Jean F.. Universite Montpellier II; Francia
Fil: Portiansky, Enrique Leo. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Olins, Donald E.. University of New England; Estados Unidos
Fil: Angel, Sergio Oscar. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Biotecnológicas. Instituto de Investigaciones Biotecnológicas "Dr. Raúl Alfonsín" (sede Chascomús). Universidad Nacional de San Martín. Instituto de Investigaciones Biotecnológicas. Instituto de Investigaciones Biotecnológicas "Dr. Raúl Alfonsín" (sede Chascomús); Argentina
Materia
Toxoplasma Gondii
Epichromatin
Nuclear Envelope
Cell Cycle
Replication
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/23200
Acceder
id CONICETDig_ef660c19ac7e733da14649bceb21292f
oai_identifier_str oai:ri.conicet.gov.ar:11336/23200
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Epichromatin is conserved in Toxoplasma gondii and labels the exterior parasite chromatin throughout the cell cycleVanagas, LauraDalmasso, Maria CarolinaDubremetz, Jean F.Portiansky, Enrique LeoOlins, Donald E.Angel, Sergio OscarToxoplasma GondiiEpichromatinNuclear EnvelopeCell CycleReplicationhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Toxoplasma gondii is an Apicomplexan intracellular protozoan parasite responsible for Toxoplasmosis, a disease with considerable medical and economic impact worldwide. T. gondii cells never lose the nuclear envelope and their chromosomes do not present a higher order level of condensation during mitosis. Here, we tested the murine monoclonal antibody PL2-6, which labels epichromatin, a conformational chromatin epitope based on DNA+H2As and H2Bs, in T. gondii cultured on fibroblast cells. This epitope is present on the exterior chromatin surface of interphase nuclei and on the periphery of metaphasic chromosomes in higher eukaryotes. PL2-6 reacted with T. gondii H2As and H2Bs histones in Western blot assays. Besides, the antibody reacted with the nuclear fraction of tachyzoites, as a single band coincident with H2B histone. In the T. gondii tachyzoite stage, PL2-6 also had peripheral nuclear localization, as observed by epifluorescence/confocal microscopy and also immunoelectron microscopy. Confocal analysis showed that epichromatin is slightly polarized to one face of the parasite exterior chromatin surface. In replicating tachyzoites, PL2-6 also labels the exterior chromatin surface, covering the face of both segregating nuclei, facing the plasma membrane of the mother cell. The possible role of epichromatin in T. gondii is discussed.Fil: Vanagas, Laura. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Biotecnológicas. Instituto de Investigaciones Biotecnológicas "Dr. Raúl Alfonsín" (sede Chascomús). Universidad Nacional de San Martín. Instituto de Investigaciones Biotecnológicas. Instituto de Investigaciones Biotecnológicas "Dr. Raúl Alfonsín" (sede Chascomús); ArgentinaFil: Dalmasso, Maria Carolina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Biotecnológicas. Instituto de Investigaciones Biotecnológicas "Dr. Raúl Alfonsín" (sede Chascomús). Universidad Nacional de San Martín. Instituto de Investigaciones Biotecnológicas. Instituto de Investigaciones Biotecnológicas "Dr. Raúl Alfonsín" (sede Chascomús); ArgentinaFil: Dubremetz, Jean F.. Universite Montpellier II; FranciaFil: Portiansky, Enrique Leo. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Olins, Donald E.. University of New England; Estados UnidosFil: Angel, Sergio Oscar. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Biotecnológicas. Instituto de Investigaciones Biotecnológicas "Dr. Raúl Alfonsín" (sede Chascomús). Universidad Nacional de San Martín. Instituto de Investigaciones Biotecnológicas. Instituto de Investigaciones Biotecnológicas "Dr. Raúl Alfonsín" (sede Chascomús); ArgentinaCambridge University Press2013-05info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/23200Vanagas, Laura; Dalmasso, Maria Carolina; Dubremetz, Jean F.; Portiansky, Enrique Leo; Olins, Donald E.; et al.; Epichromatin is conserved in Toxoplasma gondii and labels the exterior parasite chromatin throughout the cell cycle; Cambridge University Press; Parasitology; 140; 9; 5-2013; 1104-11100031-1820CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1017/S0031182013000504info:eu-repo/semantics/altIdentifier/url/https://www.cambridge.org/core/journals/parasitology/article/epichromatin-is-conserved-in-toxoplasma-gondii-and-labels-the-exterior-parasite-chromatin-throughout-the-cell-cycle/18CD42DA377C002BA1D8C81545577C3Dinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T10:05:36Zoai:ri.conicet.gov.ar:11336/23200instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 10:05:37.227CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Epichromatin is conserved in Toxoplasma gondii and labels the exterior parasite chromatin throughout the cell cycle
title Epichromatin is conserved in Toxoplasma gondii and labels the exterior parasite chromatin throughout the cell cycle
spellingShingle Epichromatin is conserved in Toxoplasma gondii and labels the exterior parasite chromatin throughout the cell cycle
Vanagas, Laura
Toxoplasma Gondii
Epichromatin
Nuclear Envelope
Cell Cycle
Replication
title_short Epichromatin is conserved in Toxoplasma gondii and labels the exterior parasite chromatin throughout the cell cycle
title_full Epichromatin is conserved in Toxoplasma gondii and labels the exterior parasite chromatin throughout the cell cycle
title_fullStr Epichromatin is conserved in Toxoplasma gondii and labels the exterior parasite chromatin throughout the cell cycle
title_full_unstemmed Epichromatin is conserved in Toxoplasma gondii and labels the exterior parasite chromatin throughout the cell cycle
title_sort Epichromatin is conserved in Toxoplasma gondii and labels the exterior parasite chromatin throughout the cell cycle
dc.creator.none.fl_str_mv Vanagas, Laura
Dalmasso, Maria Carolina
Dubremetz, Jean F.
Portiansky, Enrique Leo
Olins, Donald E.
Angel, Sergio Oscar
author Vanagas, Laura
author_facet Vanagas, Laura
Dalmasso, Maria Carolina
Dubremetz, Jean F.
Portiansky, Enrique Leo
Olins, Donald E.
Angel, Sergio Oscar
author_role author
author2 Dalmasso, Maria Carolina
Dubremetz, Jean F.
Portiansky, Enrique Leo
Olins, Donald E.
Angel, Sergio Oscar
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv Toxoplasma Gondii
Epichromatin
Nuclear Envelope
Cell Cycle
Replication
topic Toxoplasma Gondii
Epichromatin
Nuclear Envelope
Cell Cycle
Replication
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Toxoplasma gondii is an Apicomplexan intracellular protozoan parasite responsible for Toxoplasmosis, a disease with considerable medical and economic impact worldwide. T. gondii cells never lose the nuclear envelope and their chromosomes do not present a higher order level of condensation during mitosis. Here, we tested the murine monoclonal antibody PL2-6, which labels epichromatin, a conformational chromatin epitope based on DNA+H2As and H2Bs, in T. gondii cultured on fibroblast cells. This epitope is present on the exterior chromatin surface of interphase nuclei and on the periphery of metaphasic chromosomes in higher eukaryotes. PL2-6 reacted with T. gondii H2As and H2Bs histones in Western blot assays. Besides, the antibody reacted with the nuclear fraction of tachyzoites, as a single band coincident with H2B histone. In the T. gondii tachyzoite stage, PL2-6 also had peripheral nuclear localization, as observed by epifluorescence/confocal microscopy and also immunoelectron microscopy. Confocal analysis showed that epichromatin is slightly polarized to one face of the parasite exterior chromatin surface. In replicating tachyzoites, PL2-6 also labels the exterior chromatin surface, covering the face of both segregating nuclei, facing the plasma membrane of the mother cell. The possible role of epichromatin in T. gondii is discussed.
Fil: Vanagas, Laura. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Biotecnológicas. Instituto de Investigaciones Biotecnológicas "Dr. Raúl Alfonsín" (sede Chascomús). Universidad Nacional de San Martín. Instituto de Investigaciones Biotecnológicas. Instituto de Investigaciones Biotecnológicas "Dr. Raúl Alfonsín" (sede Chascomús); Argentina
Fil: Dalmasso, Maria Carolina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Biotecnológicas. Instituto de Investigaciones Biotecnológicas "Dr. Raúl Alfonsín" (sede Chascomús). Universidad Nacional de San Martín. Instituto de Investigaciones Biotecnológicas. Instituto de Investigaciones Biotecnológicas "Dr. Raúl Alfonsín" (sede Chascomús); Argentina
Fil: Dubremetz, Jean F.. Universite Montpellier II; Francia
Fil: Portiansky, Enrique Leo. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Olins, Donald E.. University of New England; Estados Unidos
Fil: Angel, Sergio Oscar. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Biotecnológicas. Instituto de Investigaciones Biotecnológicas "Dr. Raúl Alfonsín" (sede Chascomús). Universidad Nacional de San Martín. Instituto de Investigaciones Biotecnológicas. Instituto de Investigaciones Biotecnológicas "Dr. Raúl Alfonsín" (sede Chascomús); Argentina
description Toxoplasma gondii is an Apicomplexan intracellular protozoan parasite responsible for Toxoplasmosis, a disease with considerable medical and economic impact worldwide. T. gondii cells never lose the nuclear envelope and their chromosomes do not present a higher order level of condensation during mitosis. Here, we tested the murine monoclonal antibody PL2-6, which labels epichromatin, a conformational chromatin epitope based on DNA+H2As and H2Bs, in T. gondii cultured on fibroblast cells. This epitope is present on the exterior chromatin surface of interphase nuclei and on the periphery of metaphasic chromosomes in higher eukaryotes. PL2-6 reacted with T. gondii H2As and H2Bs histones in Western blot assays. Besides, the antibody reacted with the nuclear fraction of tachyzoites, as a single band coincident with H2B histone. In the T. gondii tachyzoite stage, PL2-6 also had peripheral nuclear localization, as observed by epifluorescence/confocal microscopy and also immunoelectron microscopy. Confocal analysis showed that epichromatin is slightly polarized to one face of the parasite exterior chromatin surface. In replicating tachyzoites, PL2-6 also labels the exterior chromatin surface, covering the face of both segregating nuclei, facing the plasma membrane of the mother cell. The possible role of epichromatin in T. gondii is discussed.
publishDate 2013
dc.date.none.fl_str_mv 2013-05
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/23200
Vanagas, Laura; Dalmasso, Maria Carolina; Dubremetz, Jean F.; Portiansky, Enrique Leo; Olins, Donald E.; et al.; Epichromatin is conserved in Toxoplasma gondii and labels the exterior parasite chromatin throughout the cell cycle; Cambridge University Press; Parasitology; 140; 9; 5-2013; 1104-1110
0031-1820
CONICET Digital
CONICET
url http://hdl.handle.net/11336/23200
identifier_str_mv Vanagas, Laura; Dalmasso, Maria Carolina; Dubremetz, Jean F.; Portiansky, Enrique Leo; Olins, Donald E.; et al.; Epichromatin is conserved in Toxoplasma gondii and labels the exterior parasite chromatin throughout the cell cycle; Cambridge University Press; Parasitology; 140; 9; 5-2013; 1104-1110
0031-1820
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1017/S0031182013000504
info:eu-repo/semantics/altIdentifier/url/https://www.cambridge.org/core/journals/parasitology/article/epichromatin-is-conserved-in-toxoplasma-gondii-and-labels-the-exterior-parasite-chromatin-throughout-the-cell-cycle/18CD42DA377C002BA1D8C81545577C3D
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Cambridge University Press
publisher.none.fl_str_mv Cambridge University Press
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.13397

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