Subchronic intoxication with cadmium induces morphological alterations in rat cerebellum: the protective role of a soybean diet

Autores
Martin Molinero, Glenda Daniela; Boldrini, Gabriel Giezi; López, Pablo Héctor Horacio; Alvarez, Silvina Monica
Año de publicación
2021
Idioma
inglés
Tipo de recurso
documento de conferencia
Estado
versión publicada
Descripción
Cadmium (Cd) is an environmental contaminant. The aim of this study was to characterize its toxicity in cerebellumand the potential reversal by a soybean-based diet. The glial fibrillary acid protein (GFAP), indicator for neurotoxiceffects in mature brain, was evaluated. Besides, we performed a morphometric and stereological analysis. FemaleWistar rats (n=12) were fed with casein (Cas) and soybean diets (So) as protein source for 60 days. Simultaneously,half of the animals were administered either 15 ppm of Cd in water or regular tap water as control. The cerebellumswere fixed and processed for immunofluorescence. The samples were visualized by fluorescence microscopy and thepositive fluorescence area for GFAP was quantified by IMAGE J Software. Morphometric analysis includedquantifying the number of granule cell neurons (CGn) and Purkinje cells neurons (Pkn) in serial 20μm-thick sectionsstained with cresyl violet along the different lobules. We performed a three-dimensional volumetric reconstruction ofthe tissue through the use of the software Stereo Investigator. The thicknesses of the molecular and granular layers ofthe cerebellar cortex of lobules I-X were determined on digital images. We found a decrease in the number of CGn inthe CasCd group vs. CasCo group (p<0.05) and SoCd group (p<0.01). On the contrary, the number of Pkn remainedunchanged. In addition, there was a significant reduction in the internal granular layer of CasCd group vs. SoCdgroup, while no effect was observed in the thickness of the molecular layer. The cortical thicknesses of the differentregions of each folium base from cerebellar lobules I-X did not show significant differences between the groups.Overall, these results unmask an irreversible toxic effect of low dose-sub chronic Cd intoxication on cerebellum and identify a protective role of a soy-based diet with potential as a therapeutic strategy for those individuals exposed tothis dangerous environmental contaminant.
Fil: Martin Molinero, Glenda Daniela. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto Multidisciplinario de Investigaciones Biológicas de San Luis. Universidad Nacional de San Luis. Facultad de Ciencias Físico Matemáticas y Naturales. Instituto Multidisciplinario de Investigaciones Biológicas de San Luis; Argentina
Fil: Boldrini, Gabriel Giezi. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto Multidisciplinario de Investigaciones Biológicas de San Luis. Universidad Nacional de San Luis. Facultad de Ciencias Físico Matemáticas y Naturales. Instituto Multidisciplinario de Investigaciones Biológicas de San Luis; Argentina
Fil: López, Pablo Héctor Horacio. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Centro de Investigaciones en Química Biológica de Córdoba. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Centro de Investigaciones en Química Biológica de Córdoba; Argentina
Fil: Alvarez, Silvina Monica. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto Multidisciplinario de Investigaciones Biológicas de San Luis. Universidad Nacional de San Luis. Facultad de Ciencias Físico Matemáticas y Naturales. Instituto Multidisciplinario de Investigaciones Biológicas de San Luis; Argentina
LXVI Reunión anual de la Sociedad Argentina de Investigación Clínica (SAIC); LXIX Reunión anual de la Sociedad Argentina de Immunología (SAI); LIII Reunión anual de la Asociación Argentina de Farmacología Experimental (AAFE) y XI Reunión anual de la Asociación Argentina de Nanomedicinas (NANOMED-AR)
Argentina
Sociedad Argentina de Investigación Clínica
Sociedad Argentina de Inmunología
Asociación Argentina de Farmacología Experimental
Asociación Argentina de Nanomedicinas
Materia
CADMIUM
CEREBELLUM
SOYBEAN
GFAP
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/247652
Acceder
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network_name_str CONICET Digital (CONICET)
spelling Subchronic intoxication with cadmium induces morphological alterations in rat cerebellum: the protective role of a soybean dietMartin Molinero, Glenda DanielaBoldrini, Gabriel GieziLópez, Pablo Héctor HoracioAlvarez, Silvina MonicaCADMIUMCEREBELLUMSOYBEANGFAPhttps://purl.org/becyt/ford/3.3https://purl.org/becyt/ford/3Cadmium (Cd) is an environmental contaminant. The aim of this study was to characterize its toxicity in cerebellumand the potential reversal by a soybean-based diet. The glial fibrillary acid protein (GFAP), indicator for neurotoxiceffects in mature brain, was evaluated. Besides, we performed a morphometric and stereological analysis. FemaleWistar rats (n=12) were fed with casein (Cas) and soybean diets (So) as protein source for 60 days. Simultaneously,half of the animals were administered either 15 ppm of Cd in water or regular tap water as control. The cerebellumswere fixed and processed for immunofluorescence. The samples were visualized by fluorescence microscopy and thepositive fluorescence area for GFAP was quantified by IMAGE J Software. Morphometric analysis includedquantifying the number of granule cell neurons (CGn) and Purkinje cells neurons (Pkn) in serial 20μm-thick sectionsstained with cresyl violet along the different lobules. We performed a three-dimensional volumetric reconstruction ofthe tissue through the use of the software Stereo Investigator. The thicknesses of the molecular and granular layers ofthe cerebellar cortex of lobules I-X were determined on digital images. We found a decrease in the number of CGn inthe CasCd group vs. CasCo group (p<0.05) and SoCd group (p<0.01). On the contrary, the number of Pkn remainedunchanged. In addition, there was a significant reduction in the internal granular layer of CasCd group vs. SoCdgroup, while no effect was observed in the thickness of the molecular layer. The cortical thicknesses of the differentregions of each folium base from cerebellar lobules I-X did not show significant differences between the groups.Overall, these results unmask an irreversible toxic effect of low dose-sub chronic Cd intoxication on cerebellum and identify a protective role of a soy-based diet with potential as a therapeutic strategy for those individuals exposed tothis dangerous environmental contaminant.Fil: Martin Molinero, Glenda Daniela. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto Multidisciplinario de Investigaciones Biológicas de San Luis. Universidad Nacional de San Luis. Facultad de Ciencias Físico Matemáticas y Naturales. Instituto Multidisciplinario de Investigaciones Biológicas de San Luis; ArgentinaFil: Boldrini, Gabriel Giezi. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto Multidisciplinario de Investigaciones Biológicas de San Luis. Universidad Nacional de San Luis. Facultad de Ciencias Físico Matemáticas y Naturales. Instituto Multidisciplinario de Investigaciones Biológicas de San Luis; ArgentinaFil: López, Pablo Héctor Horacio. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Centro de Investigaciones en Química Biológica de Córdoba. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Centro de Investigaciones en Química Biológica de Córdoba; ArgentinaFil: Alvarez, Silvina Monica. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto Multidisciplinario de Investigaciones Biológicas de San Luis. Universidad Nacional de San Luis. Facultad de Ciencias Físico Matemáticas y Naturales. Instituto Multidisciplinario de Investigaciones Biológicas de San Luis; ArgentinaLXVI Reunión anual de la Sociedad Argentina de Investigación Clínica (SAIC); LXIX Reunión anual de la Sociedad Argentina de Immunología (SAI); LIII Reunión anual de la Asociación Argentina de Farmacología Experimental (AAFE) y XI Reunión anual de la Asociación Argentina de Nanomedicinas (NANOMED-AR)ArgentinaSociedad Argentina de Investigación ClínicaSociedad Argentina de InmunologíaAsociación Argentina de Farmacología ExperimentalAsociación Argentina de NanomedicinasFundación Revista Medicina2021info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/conferenceObjectCongresoJournalhttp://purl.org/coar/resource_type/c_5794info:ar-repo/semantics/documentoDeConferenciaapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/247652Subchronic intoxication with cadmium induces morphological alterations in rat cerebellum: the protective role of a soybean diet; LXVI Reunión anual de la Sociedad Argentina de Investigación Clínica (SAIC); LXIX Reunión anual de la Sociedad Argentina de Immunología (SAI); LIII Reunión anual de la Asociación Argentina de Farmacología Experimental (AAFE) y XI Reunión anual de la Asociación Argentina de Nanomedicinas (NANOMED-AR); Argentina; 2021; 255-2560025-7680CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.reunionbiociencias.com.ar/wp-content/uploads/2021/11/Revista-Medicina-2021.pdfNacionalinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T09:39:00Zoai:ri.conicet.gov.ar:11336/247652instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 09:39:01.25CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Subchronic intoxication with cadmium induces morphological alterations in rat cerebellum: the protective role of a soybean diet
title Subchronic intoxication with cadmium induces morphological alterations in rat cerebellum: the protective role of a soybean diet
spellingShingle Subchronic intoxication with cadmium induces morphological alterations in rat cerebellum: the protective role of a soybean diet
Martin Molinero, Glenda Daniela
CADMIUM
CEREBELLUM
SOYBEAN
GFAP
title_short Subchronic intoxication with cadmium induces morphological alterations in rat cerebellum: the protective role of a soybean diet
title_full Subchronic intoxication with cadmium induces morphological alterations in rat cerebellum: the protective role of a soybean diet
title_fullStr Subchronic intoxication with cadmium induces morphological alterations in rat cerebellum: the protective role of a soybean diet
title_full_unstemmed Subchronic intoxication with cadmium induces morphological alterations in rat cerebellum: the protective role of a soybean diet
title_sort Subchronic intoxication with cadmium induces morphological alterations in rat cerebellum: the protective role of a soybean diet
dc.creator.none.fl_str_mv Martin Molinero, Glenda Daniela
Boldrini, Gabriel Giezi
López, Pablo Héctor Horacio
Alvarez, Silvina Monica
author Martin Molinero, Glenda Daniela
author_facet Martin Molinero, Glenda Daniela
Boldrini, Gabriel Giezi
López, Pablo Héctor Horacio
Alvarez, Silvina Monica
author_role author
author2 Boldrini, Gabriel Giezi
López, Pablo Héctor Horacio
Alvarez, Silvina Monica
author2_role author
author
author
dc.subject.none.fl_str_mv CADMIUM
CEREBELLUM
SOYBEAN
GFAP
topic CADMIUM
CEREBELLUM
SOYBEAN
GFAP
purl_subject.fl_str_mv https://purl.org/becyt/ford/3.3
https://purl.org/becyt/ford/3
dc.description.none.fl_txt_mv Cadmium (Cd) is an environmental contaminant. The aim of this study was to characterize its toxicity in cerebellumand the potential reversal by a soybean-based diet. The glial fibrillary acid protein (GFAP), indicator for neurotoxiceffects in mature brain, was evaluated. Besides, we performed a morphometric and stereological analysis. FemaleWistar rats (n=12) were fed with casein (Cas) and soybean diets (So) as protein source for 60 days. Simultaneously,half of the animals were administered either 15 ppm of Cd in water or regular tap water as control. The cerebellumswere fixed and processed for immunofluorescence. The samples were visualized by fluorescence microscopy and thepositive fluorescence area for GFAP was quantified by IMAGE J Software. Morphometric analysis includedquantifying the number of granule cell neurons (CGn) and Purkinje cells neurons (Pkn) in serial 20μm-thick sectionsstained with cresyl violet along the different lobules. We performed a three-dimensional volumetric reconstruction ofthe tissue through the use of the software Stereo Investigator. The thicknesses of the molecular and granular layers ofthe cerebellar cortex of lobules I-X were determined on digital images. We found a decrease in the number of CGn inthe CasCd group vs. CasCo group (p<0.05) and SoCd group (p<0.01). On the contrary, the number of Pkn remainedunchanged. In addition, there was a significant reduction in the internal granular layer of CasCd group vs. SoCdgroup, while no effect was observed in the thickness of the molecular layer. The cortical thicknesses of the differentregions of each folium base from cerebellar lobules I-X did not show significant differences between the groups.Overall, these results unmask an irreversible toxic effect of low dose-sub chronic Cd intoxication on cerebellum and identify a protective role of a soy-based diet with potential as a therapeutic strategy for those individuals exposed tothis dangerous environmental contaminant.
Fil: Martin Molinero, Glenda Daniela. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto Multidisciplinario de Investigaciones Biológicas de San Luis. Universidad Nacional de San Luis. Facultad de Ciencias Físico Matemáticas y Naturales. Instituto Multidisciplinario de Investigaciones Biológicas de San Luis; Argentina
Fil: Boldrini, Gabriel Giezi. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto Multidisciplinario de Investigaciones Biológicas de San Luis. Universidad Nacional de San Luis. Facultad de Ciencias Físico Matemáticas y Naturales. Instituto Multidisciplinario de Investigaciones Biológicas de San Luis; Argentina
Fil: López, Pablo Héctor Horacio. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Centro de Investigaciones en Química Biológica de Córdoba. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Centro de Investigaciones en Química Biológica de Córdoba; Argentina
Fil: Alvarez, Silvina Monica. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto Multidisciplinario de Investigaciones Biológicas de San Luis. Universidad Nacional de San Luis. Facultad de Ciencias Físico Matemáticas y Naturales. Instituto Multidisciplinario de Investigaciones Biológicas de San Luis; Argentina
LXVI Reunión anual de la Sociedad Argentina de Investigación Clínica (SAIC); LXIX Reunión anual de la Sociedad Argentina de Immunología (SAI); LIII Reunión anual de la Asociación Argentina de Farmacología Experimental (AAFE) y XI Reunión anual de la Asociación Argentina de Nanomedicinas (NANOMED-AR)
Argentina
Sociedad Argentina de Investigación Clínica
Sociedad Argentina de Inmunología
Asociación Argentina de Farmacología Experimental
Asociación Argentina de Nanomedicinas
description Cadmium (Cd) is an environmental contaminant. The aim of this study was to characterize its toxicity in cerebellumand the potential reversal by a soybean-based diet. The glial fibrillary acid protein (GFAP), indicator for neurotoxiceffects in mature brain, was evaluated. Besides, we performed a morphometric and stereological analysis. FemaleWistar rats (n=12) were fed with casein (Cas) and soybean diets (So) as protein source for 60 days. Simultaneously,half of the animals were administered either 15 ppm of Cd in water or regular tap water as control. The cerebellumswere fixed and processed for immunofluorescence. The samples were visualized by fluorescence microscopy and thepositive fluorescence area for GFAP was quantified by IMAGE J Software. Morphometric analysis includedquantifying the number of granule cell neurons (CGn) and Purkinje cells neurons (Pkn) in serial 20μm-thick sectionsstained with cresyl violet along the different lobules. We performed a three-dimensional volumetric reconstruction ofthe tissue through the use of the software Stereo Investigator. The thicknesses of the molecular and granular layers ofthe cerebellar cortex of lobules I-X were determined on digital images. We found a decrease in the number of CGn inthe CasCd group vs. CasCo group (p<0.05) and SoCd group (p<0.01). On the contrary, the number of Pkn remainedunchanged. In addition, there was a significant reduction in the internal granular layer of CasCd group vs. SoCdgroup, while no effect was observed in the thickness of the molecular layer. The cortical thicknesses of the differentregions of each folium base from cerebellar lobules I-X did not show significant differences between the groups.Overall, these results unmask an irreversible toxic effect of low dose-sub chronic Cd intoxication on cerebellum and identify a protective role of a soy-based diet with potential as a therapeutic strategy for those individuals exposed tothis dangerous environmental contaminant.
publishDate 2021
dc.date.none.fl_str_mv 2021
dc.type.none.fl_str_mv info:eu-repo/semantics/publishedVersion
info:eu-repo/semantics/conferenceObject
Congreso
Journal
http://purl.org/coar/resource_type/c_5794
info:ar-repo/semantics/documentoDeConferencia
status_str publishedVersion
format conferenceObject
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/247652
Subchronic intoxication with cadmium induces morphological alterations in rat cerebellum: the protective role of a soybean diet; LXVI Reunión anual de la Sociedad Argentina de Investigación Clínica (SAIC); LXIX Reunión anual de la Sociedad Argentina de Immunología (SAI); LIII Reunión anual de la Asociación Argentina de Farmacología Experimental (AAFE) y XI Reunión anual de la Asociación Argentina de Nanomedicinas (NANOMED-AR); Argentina; 2021; 255-256
0025-7680
CONICET Digital
CONICET
url http://hdl.handle.net/11336/247652
identifier_str_mv Subchronic intoxication with cadmium induces morphological alterations in rat cerebellum: the protective role of a soybean diet; LXVI Reunión anual de la Sociedad Argentina de Investigación Clínica (SAIC); LXIX Reunión anual de la Sociedad Argentina de Immunología (SAI); LIII Reunión anual de la Asociación Argentina de Farmacología Experimental (AAFE) y XI Reunión anual de la Asociación Argentina de Nanomedicinas (NANOMED-AR); Argentina; 2021; 255-256
0025-7680
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://www.reunionbiociencias.com.ar/wp-content/uploads/2021/11/Revista-Medicina-2021.pdf
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
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eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.coverage.none.fl_str_mv Nacional
dc.publisher.none.fl_str_mv Fundación Revista Medicina
publisher.none.fl_str_mv Fundación Revista Medicina
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
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reponame_str CONICET Digital (CONICET)
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repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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