Subchronic intoxication with cadmium induces morphological alterations in rat cerebellum: the protective role of a soybean diet
- Autores
- Martin Molinero, Glenda Daniela; Boldrini, Gabriel Giezi; López, Pablo Héctor Horacio; Alvarez, Silvina Monica
- Año de publicación
- 2021
- Idioma
- inglés
- Tipo de recurso
- documento de conferencia
- Estado
- versión publicada
- Descripción
- Cadmium (Cd) is an environmental contaminant. The aim of this study was to characterize its toxicity in cerebellumand the potential reversal by a soybean-based diet. The glial fibrillary acid protein (GFAP), indicator for neurotoxiceffects in mature brain, was evaluated. Besides, we performed a morphometric and stereological analysis. FemaleWistar rats (n=12) were fed with casein (Cas) and soybean diets (So) as protein source for 60 days. Simultaneously,half of the animals were administered either 15 ppm of Cd in water or regular tap water as control. The cerebellumswere fixed and processed for immunofluorescence. The samples were visualized by fluorescence microscopy and thepositive fluorescence area for GFAP was quantified by IMAGE J Software. Morphometric analysis includedquantifying the number of granule cell neurons (CGn) and Purkinje cells neurons (Pkn) in serial 20μm-thick sectionsstained with cresyl violet along the different lobules. We performed a three-dimensional volumetric reconstruction ofthe tissue through the use of the software Stereo Investigator. The thicknesses of the molecular and granular layers ofthe cerebellar cortex of lobules I-X were determined on digital images. We found a decrease in the number of CGn inthe CasCd group vs. CasCo group (p<0.05) and SoCd group (p<0.01). On the contrary, the number of Pkn remainedunchanged. In addition, there was a significant reduction in the internal granular layer of CasCd group vs. SoCdgroup, while no effect was observed in the thickness of the molecular layer. The cortical thicknesses of the differentregions of each folium base from cerebellar lobules I-X did not show significant differences between the groups.Overall, these results unmask an irreversible toxic effect of low dose-sub chronic Cd intoxication on cerebellum and identify a protective role of a soy-based diet with potential as a therapeutic strategy for those individuals exposed tothis dangerous environmental contaminant.
Fil: Martin Molinero, Glenda Daniela. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto Multidisciplinario de Investigaciones Biológicas de San Luis. Universidad Nacional de San Luis. Facultad de Ciencias Físico Matemáticas y Naturales. Instituto Multidisciplinario de Investigaciones Biológicas de San Luis; Argentina
Fil: Boldrini, Gabriel Giezi. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto Multidisciplinario de Investigaciones Biológicas de San Luis. Universidad Nacional de San Luis. Facultad de Ciencias Físico Matemáticas y Naturales. Instituto Multidisciplinario de Investigaciones Biológicas de San Luis; Argentina
Fil: López, Pablo Héctor Horacio. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Centro de Investigaciones en Química Biológica de Córdoba. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Centro de Investigaciones en Química Biológica de Córdoba; Argentina
Fil: Alvarez, Silvina Monica. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto Multidisciplinario de Investigaciones Biológicas de San Luis. Universidad Nacional de San Luis. Facultad de Ciencias Físico Matemáticas y Naturales. Instituto Multidisciplinario de Investigaciones Biológicas de San Luis; Argentina
LXVI Reunión anual de la Sociedad Argentina de Investigación Clínica (SAIC); LXIX Reunión anual de la Sociedad Argentina de Immunología (SAI); LIII Reunión anual de la Asociación Argentina de Farmacología Experimental (AAFE) y XI Reunión anual de la Asociación Argentina de Nanomedicinas (NANOMED-AR)
Argentina
Sociedad Argentina de Investigación Clínica
Sociedad Argentina de Inmunología
Asociación Argentina de Farmacología Experimental
Asociación Argentina de Nanomedicinas - Materia
-
CADMIUM
CEREBELLUM
SOYBEAN
GFAP - Nivel de accesibilidad
- acceso abierto
- Condiciones de uso
- https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
- Repositorio
- Institución
- Consejo Nacional de Investigaciones Científicas y Técnicas
- OAI Identificador
- oai:ri.conicet.gov.ar:11336/247652
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Subchronic intoxication with cadmium induces morphological alterations in rat cerebellum: the protective role of a soybean dietMartin Molinero, Glenda DanielaBoldrini, Gabriel GieziLópez, Pablo Héctor HoracioAlvarez, Silvina MonicaCADMIUMCEREBELLUMSOYBEANGFAPhttps://purl.org/becyt/ford/3.3https://purl.org/becyt/ford/3Cadmium (Cd) is an environmental contaminant. The aim of this study was to characterize its toxicity in cerebellumand the potential reversal by a soybean-based diet. The glial fibrillary acid protein (GFAP), indicator for neurotoxiceffects in mature brain, was evaluated. Besides, we performed a morphometric and stereological analysis. FemaleWistar rats (n=12) were fed with casein (Cas) and soybean diets (So) as protein source for 60 days. Simultaneously,half of the animals were administered either 15 ppm of Cd in water or regular tap water as control. The cerebellumswere fixed and processed for immunofluorescence. The samples were visualized by fluorescence microscopy and thepositive fluorescence area for GFAP was quantified by IMAGE J Software. Morphometric analysis includedquantifying the number of granule cell neurons (CGn) and Purkinje cells neurons (Pkn) in serial 20μm-thick sectionsstained with cresyl violet along the different lobules. We performed a three-dimensional volumetric reconstruction ofthe tissue through the use of the software Stereo Investigator. The thicknesses of the molecular and granular layers ofthe cerebellar cortex of lobules I-X were determined on digital images. We found a decrease in the number of CGn inthe CasCd group vs. CasCo group (p<0.05) and SoCd group (p<0.01). On the contrary, the number of Pkn remainedunchanged. In addition, there was a significant reduction in the internal granular layer of CasCd group vs. SoCdgroup, while no effect was observed in the thickness of the molecular layer. The cortical thicknesses of the differentregions of each folium base from cerebellar lobules I-X did not show significant differences between the groups.Overall, these results unmask an irreversible toxic effect of low dose-sub chronic Cd intoxication on cerebellum and identify a protective role of a soy-based diet with potential as a therapeutic strategy for those individuals exposed tothis dangerous environmental contaminant.Fil: Martin Molinero, Glenda Daniela. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto Multidisciplinario de Investigaciones Biológicas de San Luis. Universidad Nacional de San Luis. Facultad de Ciencias Físico Matemáticas y Naturales. Instituto Multidisciplinario de Investigaciones Biológicas de San Luis; ArgentinaFil: Boldrini, Gabriel Giezi. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto Multidisciplinario de Investigaciones Biológicas de San Luis. Universidad Nacional de San Luis. Facultad de Ciencias Físico Matemáticas y Naturales. Instituto Multidisciplinario de Investigaciones Biológicas de San Luis; ArgentinaFil: López, Pablo Héctor Horacio. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Centro de Investigaciones en Química Biológica de Córdoba. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Centro de Investigaciones en Química Biológica de Córdoba; ArgentinaFil: Alvarez, Silvina Monica. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto Multidisciplinario de Investigaciones Biológicas de San Luis. Universidad Nacional de San Luis. Facultad de Ciencias Físico Matemáticas y Naturales. Instituto Multidisciplinario de Investigaciones Biológicas de San Luis; ArgentinaLXVI Reunión anual de la Sociedad Argentina de Investigación Clínica (SAIC); LXIX Reunión anual de la Sociedad Argentina de Immunología (SAI); LIII Reunión anual de la Asociación Argentina de Farmacología Experimental (AAFE) y XI Reunión anual de la Asociación Argentina de Nanomedicinas (NANOMED-AR)ArgentinaSociedad Argentina de Investigación ClínicaSociedad Argentina de InmunologíaAsociación Argentina de Farmacología ExperimentalAsociación Argentina de NanomedicinasFundación Revista Medicina2021info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/conferenceObjectCongresoJournalhttp://purl.org/coar/resource_type/c_5794info:ar-repo/semantics/documentoDeConferenciaapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/247652Subchronic intoxication with cadmium induces morphological alterations in rat cerebellum: the protective role of a soybean diet; LXVI Reunión anual de la Sociedad Argentina de Investigación Clínica (SAIC); LXIX Reunión anual de la Sociedad Argentina de Immunología (SAI); LIII Reunión anual de la Asociación Argentina de Farmacología Experimental (AAFE) y XI Reunión anual de la Asociación Argentina de Nanomedicinas (NANOMED-AR); Argentina; 2021; 255-2560025-7680CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.reunionbiociencias.com.ar/wp-content/uploads/2021/11/Revista-Medicina-2021.pdfNacionalinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T09:47:12Zoai:ri.conicet.gov.ar:11336/247652instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 09:47:12.391CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse |
dc.title.none.fl_str_mv |
Subchronic intoxication with cadmium induces morphological alterations in rat cerebellum: the protective role of a soybean diet |
title |
Subchronic intoxication with cadmium induces morphological alterations in rat cerebellum: the protective role of a soybean diet |
spellingShingle |
Subchronic intoxication with cadmium induces morphological alterations in rat cerebellum: the protective role of a soybean diet Martin Molinero, Glenda Daniela CADMIUM CEREBELLUM SOYBEAN GFAP |
title_short |
Subchronic intoxication with cadmium induces morphological alterations in rat cerebellum: the protective role of a soybean diet |
title_full |
Subchronic intoxication with cadmium induces morphological alterations in rat cerebellum: the protective role of a soybean diet |
title_fullStr |
Subchronic intoxication with cadmium induces morphological alterations in rat cerebellum: the protective role of a soybean diet |
title_full_unstemmed |
Subchronic intoxication with cadmium induces morphological alterations in rat cerebellum: the protective role of a soybean diet |
title_sort |
Subchronic intoxication with cadmium induces morphological alterations in rat cerebellum: the protective role of a soybean diet |
dc.creator.none.fl_str_mv |
Martin Molinero, Glenda Daniela Boldrini, Gabriel Giezi López, Pablo Héctor Horacio Alvarez, Silvina Monica |
author |
Martin Molinero, Glenda Daniela |
author_facet |
Martin Molinero, Glenda Daniela Boldrini, Gabriel Giezi López, Pablo Héctor Horacio Alvarez, Silvina Monica |
author_role |
author |
author2 |
Boldrini, Gabriel Giezi López, Pablo Héctor Horacio Alvarez, Silvina Monica |
author2_role |
author author author |
dc.subject.none.fl_str_mv |
CADMIUM CEREBELLUM SOYBEAN GFAP |
topic |
CADMIUM CEREBELLUM SOYBEAN GFAP |
purl_subject.fl_str_mv |
https://purl.org/becyt/ford/3.3 https://purl.org/becyt/ford/3 |
dc.description.none.fl_txt_mv |
Cadmium (Cd) is an environmental contaminant. The aim of this study was to characterize its toxicity in cerebellumand the potential reversal by a soybean-based diet. The glial fibrillary acid protein (GFAP), indicator for neurotoxiceffects in mature brain, was evaluated. Besides, we performed a morphometric and stereological analysis. FemaleWistar rats (n=12) were fed with casein (Cas) and soybean diets (So) as protein source for 60 days. Simultaneously,half of the animals were administered either 15 ppm of Cd in water or regular tap water as control. The cerebellumswere fixed and processed for immunofluorescence. The samples were visualized by fluorescence microscopy and thepositive fluorescence area for GFAP was quantified by IMAGE J Software. Morphometric analysis includedquantifying the number of granule cell neurons (CGn) and Purkinje cells neurons (Pkn) in serial 20μm-thick sectionsstained with cresyl violet along the different lobules. We performed a three-dimensional volumetric reconstruction ofthe tissue through the use of the software Stereo Investigator. The thicknesses of the molecular and granular layers ofthe cerebellar cortex of lobules I-X were determined on digital images. We found a decrease in the number of CGn inthe CasCd group vs. CasCo group (p<0.05) and SoCd group (p<0.01). On the contrary, the number of Pkn remainedunchanged. In addition, there was a significant reduction in the internal granular layer of CasCd group vs. SoCdgroup, while no effect was observed in the thickness of the molecular layer. The cortical thicknesses of the differentregions of each folium base from cerebellar lobules I-X did not show significant differences between the groups.Overall, these results unmask an irreversible toxic effect of low dose-sub chronic Cd intoxication on cerebellum and identify a protective role of a soy-based diet with potential as a therapeutic strategy for those individuals exposed tothis dangerous environmental contaminant. Fil: Martin Molinero, Glenda Daniela. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto Multidisciplinario de Investigaciones Biológicas de San Luis. Universidad Nacional de San Luis. Facultad de Ciencias Físico Matemáticas y Naturales. Instituto Multidisciplinario de Investigaciones Biológicas de San Luis; Argentina Fil: Boldrini, Gabriel Giezi. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto Multidisciplinario de Investigaciones Biológicas de San Luis. Universidad Nacional de San Luis. Facultad de Ciencias Físico Matemáticas y Naturales. Instituto Multidisciplinario de Investigaciones Biológicas de San Luis; Argentina Fil: López, Pablo Héctor Horacio. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Centro de Investigaciones en Química Biológica de Córdoba. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Centro de Investigaciones en Química Biológica de Córdoba; Argentina Fil: Alvarez, Silvina Monica. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto Multidisciplinario de Investigaciones Biológicas de San Luis. Universidad Nacional de San Luis. Facultad de Ciencias Físico Matemáticas y Naturales. Instituto Multidisciplinario de Investigaciones Biológicas de San Luis; Argentina LXVI Reunión anual de la Sociedad Argentina de Investigación Clínica (SAIC); LXIX Reunión anual de la Sociedad Argentina de Immunología (SAI); LIII Reunión anual de la Asociación Argentina de Farmacología Experimental (AAFE) y XI Reunión anual de la Asociación Argentina de Nanomedicinas (NANOMED-AR) Argentina Sociedad Argentina de Investigación Clínica Sociedad Argentina de Inmunología Asociación Argentina de Farmacología Experimental Asociación Argentina de Nanomedicinas |
description |
Cadmium (Cd) is an environmental contaminant. The aim of this study was to characterize its toxicity in cerebellumand the potential reversal by a soybean-based diet. The glial fibrillary acid protein (GFAP), indicator for neurotoxiceffects in mature brain, was evaluated. Besides, we performed a morphometric and stereological analysis. FemaleWistar rats (n=12) were fed with casein (Cas) and soybean diets (So) as protein source for 60 days. Simultaneously,half of the animals were administered either 15 ppm of Cd in water or regular tap water as control. The cerebellumswere fixed and processed for immunofluorescence. The samples were visualized by fluorescence microscopy and thepositive fluorescence area for GFAP was quantified by IMAGE J Software. Morphometric analysis includedquantifying the number of granule cell neurons (CGn) and Purkinje cells neurons (Pkn) in serial 20μm-thick sectionsstained with cresyl violet along the different lobules. We performed a three-dimensional volumetric reconstruction ofthe tissue through the use of the software Stereo Investigator. The thicknesses of the molecular and granular layers ofthe cerebellar cortex of lobules I-X were determined on digital images. We found a decrease in the number of CGn inthe CasCd group vs. CasCo group (p<0.05) and SoCd group (p<0.01). On the contrary, the number of Pkn remainedunchanged. In addition, there was a significant reduction in the internal granular layer of CasCd group vs. SoCdgroup, while no effect was observed in the thickness of the molecular layer. The cortical thicknesses of the differentregions of each folium base from cerebellar lobules I-X did not show significant differences between the groups.Overall, these results unmask an irreversible toxic effect of low dose-sub chronic Cd intoxication on cerebellum and identify a protective role of a soy-based diet with potential as a therapeutic strategy for those individuals exposed tothis dangerous environmental contaminant. |
publishDate |
2021 |
dc.date.none.fl_str_mv |
2021 |
dc.type.none.fl_str_mv |
info:eu-repo/semantics/publishedVersion info:eu-repo/semantics/conferenceObject Congreso Journal http://purl.org/coar/resource_type/c_5794 info:ar-repo/semantics/documentoDeConferencia |
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publishedVersion |
format |
conferenceObject |
dc.identifier.none.fl_str_mv |
http://hdl.handle.net/11336/247652 Subchronic intoxication with cadmium induces morphological alterations in rat cerebellum: the protective role of a soybean diet; LXVI Reunión anual de la Sociedad Argentina de Investigación Clínica (SAIC); LXIX Reunión anual de la Sociedad Argentina de Immunología (SAI); LIII Reunión anual de la Asociación Argentina de Farmacología Experimental (AAFE) y XI Reunión anual de la Asociación Argentina de Nanomedicinas (NANOMED-AR); Argentina; 2021; 255-256 0025-7680 CONICET Digital CONICET |
url |
http://hdl.handle.net/11336/247652 |
identifier_str_mv |
Subchronic intoxication with cadmium induces morphological alterations in rat cerebellum: the protective role of a soybean diet; LXVI Reunión anual de la Sociedad Argentina de Investigación Clínica (SAIC); LXIX Reunión anual de la Sociedad Argentina de Immunología (SAI); LIII Reunión anual de la Asociación Argentina de Farmacología Experimental (AAFE) y XI Reunión anual de la Asociación Argentina de Nanomedicinas (NANOMED-AR); Argentina; 2021; 255-256 0025-7680 CONICET Digital CONICET |
dc.language.none.fl_str_mv |
eng |
language |
eng |
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info:eu-repo/semantics/altIdentifier/url/https://www.reunionbiociencias.com.ar/wp-content/uploads/2021/11/Revista-Medicina-2021.pdf |
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info:eu-repo/semantics/openAccess https://creativecommons.org/licenses/by-nc-sa/2.5/ar/ |
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openAccess |
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https://creativecommons.org/licenses/by-nc-sa/2.5/ar/ |
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Fundación Revista Medicina |
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CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas |
repository.mail.fl_str_mv |
dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar |
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