Dynamics of GABABR and associated proteins in the postnatal rat cerebellum

Autores
Vásquez, Elena; Muñoz, Estela Maris
Año de publicación
2019
Idioma
inglés
Tipo de recurso
documento de conferencia
Estado
versión publicada
Descripción
Before glutamatergic synapses are formed, GABA-mediated signaling is considered to drive cell differentiation in the developing central nervous system (CNS). GABA, a classical inhibitory neurotransmitter, can also depolarize immature cells. Although this shift is mediated by the ionotropic GABA A receptor (GABAAR), recent evidence suggests that the electrical properties of GABAARs can be modulated by the metabotropic GABA B receptor (GABABR). GABABRs are macromolecular complexes, formed by a G protein-coupled receptor and a large number of constituents that interact together and ultimately influence cell identity and behavior. The composition of these complexes exhibits wide spatiotemporal variations; however, the implications of such dynamism during development of the CNS are far from being understood. We have determined total protein expression of some constituents of GABABRs (GABABR1a; GABABR1b, GABABR2; KCTD12) in the developing cerebellum of postnatal rats at 5, 15 and 90 days after birth, by performing Western Blots. Our findings suggest that the expression levels of the core and auxiliary subunits of GABABRs vary ontogenetically. This dynamism was also observed at the mRNA levels by RT-PCR. In addition, multiple immunolabeling followed by confocal microscopy of cerebellar sections showed Purkinje cells as the most dynamic cell type in terms of subcellular localization of the different molecules studied here. Our data support a cell lineage-dependent GABABR regulation.
Fil: Vásquez, Elena. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos. Universidad Nacional de Cuyo. Facultad de Ciencias Médicas. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos; Argentina
Fil: Muñoz, Estela Maris. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos. Universidad Nacional de Cuyo. Facultad de Ciencias Médicas. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos; Argentina
XXXIII Congress of the Argentine Society for Research in Neuroscience
Córdoba
Argentina
Sociedad Argentina de Investigación en Neurociencias
Materia
GABABR
POSNATAL DEVELOPMENT
RAT
CEREBELLUM
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/215396
Acceder
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network_name_str CONICET Digital (CONICET)
spelling Dynamics of GABABR and associated proteins in the postnatal rat cerebellumVásquez, ElenaMuñoz, Estela MarisGABABRPOSNATAL DEVELOPMENTRATCEREBELLUMhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Before glutamatergic synapses are formed, GABA-mediated signaling is considered to drive cell differentiation in the developing central nervous system (CNS). GABA, a classical inhibitory neurotransmitter, can also depolarize immature cells. Although this shift is mediated by the ionotropic GABA A receptor (GABAAR), recent evidence suggests that the electrical properties of GABAARs can be modulated by the metabotropic GABA B receptor (GABABR). GABABRs are macromolecular complexes, formed by a G protein-coupled receptor and a large number of constituents that interact together and ultimately influence cell identity and behavior. The composition of these complexes exhibits wide spatiotemporal variations; however, the implications of such dynamism during development of the CNS are far from being understood. We have determined total protein expression of some constituents of GABABRs (GABABR1a; GABABR1b, GABABR2; KCTD12) in the developing cerebellum of postnatal rats at 5, 15 and 90 days after birth, by performing Western Blots. Our findings suggest that the expression levels of the core and auxiliary subunits of GABABRs vary ontogenetically. This dynamism was also observed at the mRNA levels by RT-PCR. In addition, multiple immunolabeling followed by confocal microscopy of cerebellar sections showed Purkinje cells as the most dynamic cell type in terms of subcellular localization of the different molecules studied here. Our data support a cell lineage-dependent GABABR regulation.Fil: Vásquez, Elena. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos. Universidad Nacional de Cuyo. Facultad de Ciencias Médicas. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos; ArgentinaFil: Muñoz, Estela Maris. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos. Universidad Nacional de Cuyo. Facultad de Ciencias Médicas. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos; ArgentinaXXXIII Congress of the Argentine Society for Research in NeuroscienceCórdobaArgentinaSociedad Argentina de Investigación en NeurocienciasSAGE Publications2019info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/conferenceObjectCongresoJournalhttp://purl.org/coar/resource_type/c_5794info:ar-repo/semantics/documentoDeConferenciaapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/215396Dynamics of GABABR and associated proteins in the postnatal rat cerebellum; XXXIII Congress of the Argentine Society for Research in Neuroscience; Córdoba; Argentina; 2018; 76-761759-0914CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://journals.sagepub.com/doi/10.1177/1759091419834821info:eu-repo/semantics/altIdentifier/doi/10.1177/1759091419834821Internacionalinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T10:00:38Zoai:ri.conicet.gov.ar:11336/215396instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 10:00:39.104CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Dynamics of GABABR and associated proteins in the postnatal rat cerebellum
title Dynamics of GABABR and associated proteins in the postnatal rat cerebellum
spellingShingle Dynamics of GABABR and associated proteins in the postnatal rat cerebellum
Vásquez, Elena
GABABR
POSNATAL DEVELOPMENT
RAT
CEREBELLUM
title_short Dynamics of GABABR and associated proteins in the postnatal rat cerebellum
title_full Dynamics of GABABR and associated proteins in the postnatal rat cerebellum
title_fullStr Dynamics of GABABR and associated proteins in the postnatal rat cerebellum
title_full_unstemmed Dynamics of GABABR and associated proteins in the postnatal rat cerebellum
title_sort Dynamics of GABABR and associated proteins in the postnatal rat cerebellum
dc.creator.none.fl_str_mv Vásquez, Elena
Muñoz, Estela Maris
author Vásquez, Elena
author_facet Vásquez, Elena
Muñoz, Estela Maris
author_role author
author2 Muñoz, Estela Maris
author2_role author
dc.subject.none.fl_str_mv GABABR
POSNATAL DEVELOPMENT
RAT
CEREBELLUM
topic GABABR
POSNATAL DEVELOPMENT
RAT
CEREBELLUM
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Before glutamatergic synapses are formed, GABA-mediated signaling is considered to drive cell differentiation in the developing central nervous system (CNS). GABA, a classical inhibitory neurotransmitter, can also depolarize immature cells. Although this shift is mediated by the ionotropic GABA A receptor (GABAAR), recent evidence suggests that the electrical properties of GABAARs can be modulated by the metabotropic GABA B receptor (GABABR). GABABRs are macromolecular complexes, formed by a G protein-coupled receptor and a large number of constituents that interact together and ultimately influence cell identity and behavior. The composition of these complexes exhibits wide spatiotemporal variations; however, the implications of such dynamism during development of the CNS are far from being understood. We have determined total protein expression of some constituents of GABABRs (GABABR1a; GABABR1b, GABABR2; KCTD12) in the developing cerebellum of postnatal rats at 5, 15 and 90 days after birth, by performing Western Blots. Our findings suggest that the expression levels of the core and auxiliary subunits of GABABRs vary ontogenetically. This dynamism was also observed at the mRNA levels by RT-PCR. In addition, multiple immunolabeling followed by confocal microscopy of cerebellar sections showed Purkinje cells as the most dynamic cell type in terms of subcellular localization of the different molecules studied here. Our data support a cell lineage-dependent GABABR regulation.
Fil: Vásquez, Elena. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos. Universidad Nacional de Cuyo. Facultad de Ciencias Médicas. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos; Argentina
Fil: Muñoz, Estela Maris. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos. Universidad Nacional de Cuyo. Facultad de Ciencias Médicas. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos; Argentina
XXXIII Congress of the Argentine Society for Research in Neuroscience
Córdoba
Argentina
Sociedad Argentina de Investigación en Neurociencias
description Before glutamatergic synapses are formed, GABA-mediated signaling is considered to drive cell differentiation in the developing central nervous system (CNS). GABA, a classical inhibitory neurotransmitter, can also depolarize immature cells. Although this shift is mediated by the ionotropic GABA A receptor (GABAAR), recent evidence suggests that the electrical properties of GABAARs can be modulated by the metabotropic GABA B receptor (GABABR). GABABRs are macromolecular complexes, formed by a G protein-coupled receptor and a large number of constituents that interact together and ultimately influence cell identity and behavior. The composition of these complexes exhibits wide spatiotemporal variations; however, the implications of such dynamism during development of the CNS are far from being understood. We have determined total protein expression of some constituents of GABABRs (GABABR1a; GABABR1b, GABABR2; KCTD12) in the developing cerebellum of postnatal rats at 5, 15 and 90 days after birth, by performing Western Blots. Our findings suggest that the expression levels of the core and auxiliary subunits of GABABRs vary ontogenetically. This dynamism was also observed at the mRNA levels by RT-PCR. In addition, multiple immunolabeling followed by confocal microscopy of cerebellar sections showed Purkinje cells as the most dynamic cell type in terms of subcellular localization of the different molecules studied here. Our data support a cell lineage-dependent GABABR regulation.
publishDate 2019
dc.date.none.fl_str_mv 2019
dc.type.none.fl_str_mv info:eu-repo/semantics/publishedVersion
info:eu-repo/semantics/conferenceObject
Congreso
Journal
http://purl.org/coar/resource_type/c_5794
info:ar-repo/semantics/documentoDeConferencia
status_str publishedVersion
format conferenceObject
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/215396
Dynamics of GABABR and associated proteins in the postnatal rat cerebellum; XXXIII Congress of the Argentine Society for Research in Neuroscience; Córdoba; Argentina; 2018; 76-76
1759-0914
CONICET Digital
CONICET
url http://hdl.handle.net/11336/215396
identifier_str_mv Dynamics of GABABR and associated proteins in the postnatal rat cerebellum; XXXIII Congress of the Argentine Society for Research in Neuroscience; Córdoba; Argentina; 2018; 76-76
1759-0914
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://journals.sagepub.com/doi/10.1177/1759091419834821
info:eu-repo/semantics/altIdentifier/doi/10.1177/1759091419834821
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
dc.coverage.none.fl_str_mv Internacional
dc.publisher.none.fl_str_mv SAGE Publications
publisher.none.fl_str_mv SAGE Publications
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
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instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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