Effects of a Subchronic Intoxication with Cadmium in the Hippocampus of Rats Fed a Soybean-Based Diet

Autores
Plateo Pignatari, Maria Gabriela; Della Vedova, Maria Cecilia; Perez Diaz MF; Navigatore Fonzo, Lorena Silvina; Sanchez, S.E.; Gimenez, Maria Sofia; Ramirez, Dario
Año de publicación
2018
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Experimental and population studies have shown that sub chronic exposure tocadmium (Cd) causes severe toxic effects in several organs including in the brain,but information on the effects in the hippocampus (Hp) is rare. Soybean is animportant source of protein and isoflavones. Herein we hypothesized that a subchronic cadmium-intoxication throughout the drinking water can cause oxidative/inflammation changes and apoptosis in the Hp; and that a soybean-based diet(SBD) can modulate these effects. To accomplish this goal, we fed 4 groups offemale Wistar-rats for 60 days as follows: casein-based diet + tap water (CBD);CBD + tap water with 15 ppm Cd (CBD + Cd); soybean-based diet + tap water(SBD); and SBD + tap water with 15 ppm Cd (SBD + Cd). After the treatment,molecular markers of oxidative stress, inflammation, and apoptosis were measuredin serum and Hp tissue. Compared to the Hp of CBD fed rats, the Hp of CBDCd showed an apoptotic profle (high p53 and high Bax / Bcl-2 ratio). However,an SBD caused a pro-apoptosis pattern, i.e, increased p53 and Bax expression,reduced Bcl-2 expression and increased Bax / Bcl-2 ratio. Regardless increasedantioxidant enzymes, Cd exposure in SBD fed rats had a synergistic effect in lipidperoxidation and protein oxidation in the Hp. Taking together our data suggest thatan SBD and Cd-exposure have a synergistic effect on oxidative stress/inflammationand apoptosis in Hp. Caution may be taken on using soybean as a source of proteinin patients exposed to Cd.
Fil: Plateo Pignatari, Maria Gabriela. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto Multidisciplinario de Investigaciones Biológicas de San Luis. Universidad Nacional de San Luis. Facultad de Ciencias Físico Matemáticas y Naturales. Instituto Multidisciplinario de Investigaciones Biológicas de San Luis; Argentina
Fil: Della Vedova, Maria Cecilia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto de Química de San Luis. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia; Argentina; Argentina
Fil: Perez Diaz MF. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto de Química de San Luis. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia; Argentina; Argentina
Fil: Navigatore Fonzo, Lorena Silvina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto Multidisciplinario de Investigaciones Biológicas de San Luis. Universidad Nacional de San Luis. Facultad de Ciencias Físico Matemáticas y Naturales. Instituto Multidisciplinario de Investigaciones Biológicas de San Luis; Argentina
Fil: Sanchez, S.E.. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto Multidisciplinario de Investigaciones Biológicas de San Luis. Universidad Nacional de San Luis. Facultad de Ciencias Físico Matemáticas y Naturales. Instituto Multidisciplinario de Investigaciones Biológicas de San Luis; Argentina
Fil: Gimenez, Maria Sofia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto Multidisciplinario de Investigaciones Biológicas de San Luis. Universidad Nacional de San Luis. Facultad de Ciencias Físico Matemáticas y Naturales. Instituto Multidisciplinario de Investigaciones Biológicas de San Luis; Argentina
Fil: Ramirez, Dario. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto Multidisciplinario de Investigaciones Biológicas de San Luis. Universidad Nacional de San Luis. Facultad de Ciencias Físico Matemáticas y Naturales. Instituto Multidisciplinario de Investigaciones Biológicas de San Luis; Argentina
Materia
SOY-BASED DIET
OXIDATIVE STRESS
HIPPOCAMPUS
INFLAMMATION
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/87601
Acceder
id CONICETDig_c866983203484242bd12ffcec569b204
oai_identifier_str oai:ri.conicet.gov.ar:11336/87601
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Effects of a Subchronic Intoxication with Cadmium in the Hippocampus of Rats Fed a Soybean-Based DietPlateo Pignatari, Maria GabrielaDella Vedova, Maria CeciliaPerez Diaz MFNavigatore Fonzo, Lorena SilvinaSanchez, S.E.Gimenez, Maria SofiaRamirez, DarioSOY-BASED DIETOXIDATIVE STRESSHIPPOCAMPUSINFLAMMATIONhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Experimental and population studies have shown that sub chronic exposure tocadmium (Cd) causes severe toxic effects in several organs including in the brain,but information on the effects in the hippocampus (Hp) is rare. Soybean is animportant source of protein and isoflavones. Herein we hypothesized that a subchronic cadmium-intoxication throughout the drinking water can cause oxidative/inflammation changes and apoptosis in the Hp; and that a soybean-based diet(SBD) can modulate these effects. To accomplish this goal, we fed 4 groups offemale Wistar-rats for 60 days as follows: casein-based diet + tap water (CBD);CBD + tap water with 15 ppm Cd (CBD + Cd); soybean-based diet + tap water(SBD); and SBD + tap water with 15 ppm Cd (SBD + Cd). After the treatment,molecular markers of oxidative stress, inflammation, and apoptosis were measuredin serum and Hp tissue. Compared to the Hp of CBD fed rats, the Hp of CBDCd showed an apoptotic profle (high p53 and high Bax / Bcl-2 ratio). However,an SBD caused a pro-apoptosis pattern, i.e, increased p53 and Bax expression,reduced Bcl-2 expression and increased Bax / Bcl-2 ratio. Regardless increasedantioxidant enzymes, Cd exposure in SBD fed rats had a synergistic effect in lipidperoxidation and protein oxidation in the Hp. Taking together our data suggest thatan SBD and Cd-exposure have a synergistic effect on oxidative stress/inflammationand apoptosis in Hp. Caution may be taken on using soybean as a source of proteinin patients exposed to Cd.Fil: Plateo Pignatari, Maria Gabriela. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto Multidisciplinario de Investigaciones Biológicas de San Luis. Universidad Nacional de San Luis. Facultad de Ciencias Físico Matemáticas y Naturales. Instituto Multidisciplinario de Investigaciones Biológicas de San Luis; ArgentinaFil: Della Vedova, Maria Cecilia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto de Química de San Luis. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia; Argentina; ArgentinaFil: Perez Diaz MF. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto de Química de San Luis. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia; Argentina; ArgentinaFil: Navigatore Fonzo, Lorena Silvina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto Multidisciplinario de Investigaciones Biológicas de San Luis. Universidad Nacional de San Luis. Facultad de Ciencias Físico Matemáticas y Naturales. Instituto Multidisciplinario de Investigaciones Biológicas de San Luis; ArgentinaFil: Sanchez, S.E.. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto Multidisciplinario de Investigaciones Biológicas de San Luis. Universidad Nacional de San Luis. Facultad de Ciencias Físico Matemáticas y Naturales. Instituto Multidisciplinario de Investigaciones Biológicas de San Luis; ArgentinaFil: Gimenez, Maria Sofia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto Multidisciplinario de Investigaciones Biológicas de San Luis. Universidad Nacional de San Luis. Facultad de Ciencias Físico Matemáticas y Naturales. Instituto Multidisciplinario de Investigaciones Biológicas de San Luis; ArgentinaFil: Ramirez, Dario. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto Multidisciplinario de Investigaciones Biológicas de San Luis. Universidad Nacional de San Luis. Facultad de Ciencias Físico Matemáticas y Naturales. Instituto Multidisciplinario de Investigaciones Biológicas de San Luis; ArgentinaApplied Cell Biology2018-12info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/87601Plateo Pignatari, Maria Gabriela; Della Vedova, Maria Cecilia; Perez Diaz MF; Navigatore Fonzo, Lorena Silvina; Sanchez, S.E.; et al.; Effects of a Subchronic Intoxication with Cadmium in the Hippocampus of Rats Fed a Soybean-Based Diet; Applied Cell Biology; Applied Cell Biology; 6; 2; 12-2018; 18-332320-19832320-1991CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/http://www.appliedcellbiology.com/articles/123/effects-of-a-subchronic-intoxication-with-cadmium-in-the-hippocampus-of-rats-fed-a-soybean-based-dietinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-15T15:22:46Zoai:ri.conicet.gov.ar:11336/87601instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-15 15:22:46.532CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Effects of a Subchronic Intoxication with Cadmium in the Hippocampus of Rats Fed a Soybean-Based Diet
title Effects of a Subchronic Intoxication with Cadmium in the Hippocampus of Rats Fed a Soybean-Based Diet
spellingShingle Effects of a Subchronic Intoxication with Cadmium in the Hippocampus of Rats Fed a Soybean-Based Diet
Plateo Pignatari, Maria Gabriela
SOY-BASED DIET
OXIDATIVE STRESS
HIPPOCAMPUS
INFLAMMATION
title_short Effects of a Subchronic Intoxication with Cadmium in the Hippocampus of Rats Fed a Soybean-Based Diet
title_full Effects of a Subchronic Intoxication with Cadmium in the Hippocampus of Rats Fed a Soybean-Based Diet
title_fullStr Effects of a Subchronic Intoxication with Cadmium in the Hippocampus of Rats Fed a Soybean-Based Diet
title_full_unstemmed Effects of a Subchronic Intoxication with Cadmium in the Hippocampus of Rats Fed a Soybean-Based Diet
title_sort Effects of a Subchronic Intoxication with Cadmium in the Hippocampus of Rats Fed a Soybean-Based Diet
dc.creator.none.fl_str_mv Plateo Pignatari, Maria Gabriela
Della Vedova, Maria Cecilia
Perez Diaz MF
Navigatore Fonzo, Lorena Silvina
Sanchez, S.E.
Gimenez, Maria Sofia
Ramirez, Dario
author Plateo Pignatari, Maria Gabriela
author_facet Plateo Pignatari, Maria Gabriela
Della Vedova, Maria Cecilia
Perez Diaz MF
Navigatore Fonzo, Lorena Silvina
Sanchez, S.E.
Gimenez, Maria Sofia
Ramirez, Dario
author_role author
author2 Della Vedova, Maria Cecilia
Perez Diaz MF
Navigatore Fonzo, Lorena Silvina
Sanchez, S.E.
Gimenez, Maria Sofia
Ramirez, Dario
author2_role author
author
author
author
author
author
dc.subject.none.fl_str_mv SOY-BASED DIET
OXIDATIVE STRESS
HIPPOCAMPUS
INFLAMMATION
topic SOY-BASED DIET
OXIDATIVE STRESS
HIPPOCAMPUS
INFLAMMATION
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Experimental and population studies have shown that sub chronic exposure tocadmium (Cd) causes severe toxic effects in several organs including in the brain,but information on the effects in the hippocampus (Hp) is rare. Soybean is animportant source of protein and isoflavones. Herein we hypothesized that a subchronic cadmium-intoxication throughout the drinking water can cause oxidative/inflammation changes and apoptosis in the Hp; and that a soybean-based diet(SBD) can modulate these effects. To accomplish this goal, we fed 4 groups offemale Wistar-rats for 60 days as follows: casein-based diet + tap water (CBD);CBD + tap water with 15 ppm Cd (CBD + Cd); soybean-based diet + tap water(SBD); and SBD + tap water with 15 ppm Cd (SBD + Cd). After the treatment,molecular markers of oxidative stress, inflammation, and apoptosis were measuredin serum and Hp tissue. Compared to the Hp of CBD fed rats, the Hp of CBDCd showed an apoptotic profle (high p53 and high Bax / Bcl-2 ratio). However,an SBD caused a pro-apoptosis pattern, i.e, increased p53 and Bax expression,reduced Bcl-2 expression and increased Bax / Bcl-2 ratio. Regardless increasedantioxidant enzymes, Cd exposure in SBD fed rats had a synergistic effect in lipidperoxidation and protein oxidation in the Hp. Taking together our data suggest thatan SBD and Cd-exposure have a synergistic effect on oxidative stress/inflammationand apoptosis in Hp. Caution may be taken on using soybean as a source of proteinin patients exposed to Cd.
Fil: Plateo Pignatari, Maria Gabriela. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto Multidisciplinario de Investigaciones Biológicas de San Luis. Universidad Nacional de San Luis. Facultad de Ciencias Físico Matemáticas y Naturales. Instituto Multidisciplinario de Investigaciones Biológicas de San Luis; Argentina
Fil: Della Vedova, Maria Cecilia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto de Química de San Luis. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia; Argentina; Argentina
Fil: Perez Diaz MF. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto de Química de San Luis. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia; Argentina; Argentina
Fil: Navigatore Fonzo, Lorena Silvina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto Multidisciplinario de Investigaciones Biológicas de San Luis. Universidad Nacional de San Luis. Facultad de Ciencias Físico Matemáticas y Naturales. Instituto Multidisciplinario de Investigaciones Biológicas de San Luis; Argentina
Fil: Sanchez, S.E.. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto Multidisciplinario de Investigaciones Biológicas de San Luis. Universidad Nacional de San Luis. Facultad de Ciencias Físico Matemáticas y Naturales. Instituto Multidisciplinario de Investigaciones Biológicas de San Luis; Argentina
Fil: Gimenez, Maria Sofia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto Multidisciplinario de Investigaciones Biológicas de San Luis. Universidad Nacional de San Luis. Facultad de Ciencias Físico Matemáticas y Naturales. Instituto Multidisciplinario de Investigaciones Biológicas de San Luis; Argentina
Fil: Ramirez, Dario. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto Multidisciplinario de Investigaciones Biológicas de San Luis. Universidad Nacional de San Luis. Facultad de Ciencias Físico Matemáticas y Naturales. Instituto Multidisciplinario de Investigaciones Biológicas de San Luis; Argentina
description Experimental and population studies have shown that sub chronic exposure tocadmium (Cd) causes severe toxic effects in several organs including in the brain,but information on the effects in the hippocampus (Hp) is rare. Soybean is animportant source of protein and isoflavones. Herein we hypothesized that a subchronic cadmium-intoxication throughout the drinking water can cause oxidative/inflammation changes and apoptosis in the Hp; and that a soybean-based diet(SBD) can modulate these effects. To accomplish this goal, we fed 4 groups offemale Wistar-rats for 60 days as follows: casein-based diet + tap water (CBD);CBD + tap water with 15 ppm Cd (CBD + Cd); soybean-based diet + tap water(SBD); and SBD + tap water with 15 ppm Cd (SBD + Cd). After the treatment,molecular markers of oxidative stress, inflammation, and apoptosis were measuredin serum and Hp tissue. Compared to the Hp of CBD fed rats, the Hp of CBDCd showed an apoptotic profle (high p53 and high Bax / Bcl-2 ratio). However,an SBD caused a pro-apoptosis pattern, i.e, increased p53 and Bax expression,reduced Bcl-2 expression and increased Bax / Bcl-2 ratio. Regardless increasedantioxidant enzymes, Cd exposure in SBD fed rats had a synergistic effect in lipidperoxidation and protein oxidation in the Hp. Taking together our data suggest thatan SBD and Cd-exposure have a synergistic effect on oxidative stress/inflammationand apoptosis in Hp. Caution may be taken on using soybean as a source of proteinin patients exposed to Cd.
publishDate 2018
dc.date.none.fl_str_mv 2018-12
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/87601
Plateo Pignatari, Maria Gabriela; Della Vedova, Maria Cecilia; Perez Diaz MF; Navigatore Fonzo, Lorena Silvina; Sanchez, S.E.; et al.; Effects of a Subchronic Intoxication with Cadmium in the Hippocampus of Rats Fed a Soybean-Based Diet; Applied Cell Biology; Applied Cell Biology; 6; 2; 12-2018; 18-33
2320-1983
2320-1991
CONICET Digital
CONICET
url http://hdl.handle.net/11336/87601
identifier_str_mv Plateo Pignatari, Maria Gabriela; Della Vedova, Maria Cecilia; Perez Diaz MF; Navigatore Fonzo, Lorena Silvina; Sanchez, S.E.; et al.; Effects of a Subchronic Intoxication with Cadmium in the Hippocampus of Rats Fed a Soybean-Based Diet; Applied Cell Biology; Applied Cell Biology; 6; 2; 12-2018; 18-33
2320-1983
2320-1991
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/http://www.appliedcellbiology.com/articles/123/effects-of-a-subchronic-intoxication-with-cadmium-in-the-hippocampus-of-rats-fed-a-soybean-based-diet
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Applied Cell Biology
publisher.none.fl_str_mv Applied Cell Biology
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.22299

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