2-iodohexadecanal Inhibits thyroid cell growth in part through the induction of let-7f microRNA

Autores
Salvarredi, Leonardo Andres; Thomasz, Lisa; Rossich, Luciano Esteban; Saiselet, Manuel; Pisarev, Mario Alberto; Fusco, Alfredo; Juvenal, Guillermo Juan
Año de publicación
2015
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
It is well known that pituitary TSH exerts the major task in the regulation of thyroid function. However, this gland is capable of certain degree of autonomy, independently of TSH control. Iodine plays an important role in thyroid physiology and biochemistry. The thyroid is capable of producing different iodolipids such as 2-iodohexadecanal (2-IHDA). It was shown that this iodolipid mimic some of the inhibitory effects of excess iodide on several thyroid parameters. Objectives: To identify the miRNAs regulated by 2-IHDA in rat thyroid cells and likely characterize their role in thyroid cell proliferation and function. Results: FRTL-5 cells were grown in the presence of TSH and treated with 2-IHDA. Among the miRNAs up-regulated by 2-IHDA we focused on miR-let-7f and miR-138. When we transfected the miRNAs, miR-let-7f but not miR-138 overexpression inhibited proliferation of FRTL 5 cells, while miR-let-7f inhibition restored cell growth in 2-IHDA treated cultures. Analysis of cell cycle by flow cytometric DNA analysis revealed that miR-let-7f inhibition reduced the percentage of 2-IHDA treated cells in G1 phase and an increased of the percentage of cells in S phase was observed upon anti-let-7f transfection. The expresion of Cyclin D1 and Cyclin D3 were reduced after the transfection of miR-let-7f and miR-138, respectively. In in vivo studies we observed that miR-let-7f and miR-138 were up regulated by 2-IHDA during goiter involution. Conclusion: These results suggest that the inhibitory effects of 2-IHDA on FRTL-5 thyroid cell proliferation are mediated in part through the induction of let-7f microRNA.
Fil: Salvarredi, Leonardo Andres. Comisión Nacional de Energía Atómica. Gerencia de Área de Aplicaciones de la Tecnología Nuclear. División Bioquímica Nuclear; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Thomasz, Lisa. Comisión Nacional de Energía Atómica. Gerencia de Área de Aplicaciones de la Tecnología Nuclear. División Bioquímica Nuclear; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Rossich, Luciano Esteban. Comisión Nacional de Energía Atómica. Gerencia de Área de Aplicaciones de la Tecnología Nuclear. División Bioquímica Nuclear; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Saiselet, Manuel. Université Libre de Bruxelles; Bélgica
Fil: Pisarev, Mario Alberto. Comisión Nacional de Energía Atómica. Gerencia de Área de Aplicaciones de la Tecnología Nuclear. División Bioquímica Nuclear; Argentina. Universidad de Buenos Aires. Facultad de Medicina. Departamento de Bioquímica Humana; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Fusco, Alfredo. Università degli Studi di Napoli Federico II; Italia
Fil: Juvenal, Guillermo Juan. Comisión Nacional de Energía Atómica. Gerencia de Área de Aplicaciones de la Tecnología Nuclear. División Bioquímica Nuclear; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Materia
Tiroides
Micrornas
Iodolípidos
Cáncer
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/42259
Acceder
id CONICETDig_ef4cb154cce880a5b046a5efa9801927
oai_identifier_str oai:ri.conicet.gov.ar:11336/42259
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling 2-iodohexadecanal Inhibits thyroid cell growth in part through the induction of let-7f microRNASalvarredi, Leonardo AndresThomasz, LisaRossich, Luciano EstebanSaiselet, ManuelPisarev, Mario AlbertoFusco, AlfredoJuvenal, Guillermo JuanTiroidesMicrornasIodolípidosCáncerhttps://purl.org/becyt/ford/3.1https://purl.org/becyt/ford/3It is well known that pituitary TSH exerts the major task in the regulation of thyroid function. However, this gland is capable of certain degree of autonomy, independently of TSH control. Iodine plays an important role in thyroid physiology and biochemistry. The thyroid is capable of producing different iodolipids such as 2-iodohexadecanal (2-IHDA). It was shown that this iodolipid mimic some of the inhibitory effects of excess iodide on several thyroid parameters. Objectives: To identify the miRNAs regulated by 2-IHDA in rat thyroid cells and likely characterize their role in thyroid cell proliferation and function. Results: FRTL-5 cells were grown in the presence of TSH and treated with 2-IHDA. Among the miRNAs up-regulated by 2-IHDA we focused on miR-let-7f and miR-138. When we transfected the miRNAs, miR-let-7f but not miR-138 overexpression inhibited proliferation of FRTL 5 cells, while miR-let-7f inhibition restored cell growth in 2-IHDA treated cultures. Analysis of cell cycle by flow cytometric DNA analysis revealed that miR-let-7f inhibition reduced the percentage of 2-IHDA treated cells in G1 phase and an increased of the percentage of cells in S phase was observed upon anti-let-7f transfection. The expresion of Cyclin D1 and Cyclin D3 were reduced after the transfection of miR-let-7f and miR-138, respectively. In in vivo studies we observed that miR-let-7f and miR-138 were up regulated by 2-IHDA during goiter involution. Conclusion: These results suggest that the inhibitory effects of 2-IHDA on FRTL-5 thyroid cell proliferation are mediated in part through the induction of let-7f microRNA.Fil: Salvarredi, Leonardo Andres. Comisión Nacional de Energía Atómica. Gerencia de Área de Aplicaciones de la Tecnología Nuclear. División Bioquímica Nuclear; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Thomasz, Lisa. Comisión Nacional de Energía Atómica. Gerencia de Área de Aplicaciones de la Tecnología Nuclear. División Bioquímica Nuclear; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Rossich, Luciano Esteban. Comisión Nacional de Energía Atómica. Gerencia de Área de Aplicaciones de la Tecnología Nuclear. División Bioquímica Nuclear; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Saiselet, Manuel. Université Libre de Bruxelles; BélgicaFil: Pisarev, Mario Alberto. Comisión Nacional de Energía Atómica. Gerencia de Área de Aplicaciones de la Tecnología Nuclear. División Bioquímica Nuclear; Argentina. Universidad de Buenos Aires. Facultad de Medicina. Departamento de Bioquímica Humana; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Fusco, Alfredo. Università degli Studi di Napoli Federico II; ItaliaFil: Juvenal, Guillermo Juan. Comisión Nacional de Energía Atómica. Gerencia de Área de Aplicaciones de la Tecnología Nuclear. División Bioquímica Nuclear; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaElsevier Ireland2015-10info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/42259Salvarredi, Leonardo Andres; Thomasz, Lisa; Rossich, Luciano Esteban; Saiselet, Manuel; Pisarev, Mario Alberto; et al.; 2-iodohexadecanal Inhibits thyroid cell growth in part through the induction of let-7f microRNA; Elsevier Ireland; Molecular and Cellular Endocrinology; 414; 10-2015; 224-2320303-7207CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1016/j.mce.2015.07.007info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S0303720715300162info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-nd/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-22T11:00:20Zoai:ri.conicet.gov.ar:11336/42259instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-22 11:00:21.167CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv 2-iodohexadecanal Inhibits thyroid cell growth in part through the induction of let-7f microRNA
title 2-iodohexadecanal Inhibits thyroid cell growth in part through the induction of let-7f microRNA
spellingShingle 2-iodohexadecanal Inhibits thyroid cell growth in part through the induction of let-7f microRNA
Salvarredi, Leonardo Andres
Tiroides
Micrornas
Iodolípidos
Cáncer
title_short 2-iodohexadecanal Inhibits thyroid cell growth in part through the induction of let-7f microRNA
title_full 2-iodohexadecanal Inhibits thyroid cell growth in part through the induction of let-7f microRNA
title_fullStr 2-iodohexadecanal Inhibits thyroid cell growth in part through the induction of let-7f microRNA
title_full_unstemmed 2-iodohexadecanal Inhibits thyroid cell growth in part through the induction of let-7f microRNA
title_sort 2-iodohexadecanal Inhibits thyroid cell growth in part through the induction of let-7f microRNA
dc.creator.none.fl_str_mv Salvarredi, Leonardo Andres
Thomasz, Lisa
Rossich, Luciano Esteban
Saiselet, Manuel
Pisarev, Mario Alberto
Fusco, Alfredo
Juvenal, Guillermo Juan
author Salvarredi, Leonardo Andres
author_facet Salvarredi, Leonardo Andres
Thomasz, Lisa
Rossich, Luciano Esteban
Saiselet, Manuel
Pisarev, Mario Alberto
Fusco, Alfredo
Juvenal, Guillermo Juan
author_role author
author2 Thomasz, Lisa
Rossich, Luciano Esteban
Saiselet, Manuel
Pisarev, Mario Alberto
Fusco, Alfredo
Juvenal, Guillermo Juan
author2_role author
author
author
author
author
author
dc.subject.none.fl_str_mv Tiroides
Micrornas
Iodolípidos
Cáncer
topic Tiroides
Micrornas
Iodolípidos
Cáncer
purl_subject.fl_str_mv https://purl.org/becyt/ford/3.1
https://purl.org/becyt/ford/3
dc.description.none.fl_txt_mv It is well known that pituitary TSH exerts the major task in the regulation of thyroid function. However, this gland is capable of certain degree of autonomy, independently of TSH control. Iodine plays an important role in thyroid physiology and biochemistry. The thyroid is capable of producing different iodolipids such as 2-iodohexadecanal (2-IHDA). It was shown that this iodolipid mimic some of the inhibitory effects of excess iodide on several thyroid parameters. Objectives: To identify the miRNAs regulated by 2-IHDA in rat thyroid cells and likely characterize their role in thyroid cell proliferation and function. Results: FRTL-5 cells were grown in the presence of TSH and treated with 2-IHDA. Among the miRNAs up-regulated by 2-IHDA we focused on miR-let-7f and miR-138. When we transfected the miRNAs, miR-let-7f but not miR-138 overexpression inhibited proliferation of FRTL 5 cells, while miR-let-7f inhibition restored cell growth in 2-IHDA treated cultures. Analysis of cell cycle by flow cytometric DNA analysis revealed that miR-let-7f inhibition reduced the percentage of 2-IHDA treated cells in G1 phase and an increased of the percentage of cells in S phase was observed upon anti-let-7f transfection. The expresion of Cyclin D1 and Cyclin D3 were reduced after the transfection of miR-let-7f and miR-138, respectively. In in vivo studies we observed that miR-let-7f and miR-138 were up regulated by 2-IHDA during goiter involution. Conclusion: These results suggest that the inhibitory effects of 2-IHDA on FRTL-5 thyroid cell proliferation are mediated in part through the induction of let-7f microRNA.
Fil: Salvarredi, Leonardo Andres. Comisión Nacional de Energía Atómica. Gerencia de Área de Aplicaciones de la Tecnología Nuclear. División Bioquímica Nuclear; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Thomasz, Lisa. Comisión Nacional de Energía Atómica. Gerencia de Área de Aplicaciones de la Tecnología Nuclear. División Bioquímica Nuclear; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Rossich, Luciano Esteban. Comisión Nacional de Energía Atómica. Gerencia de Área de Aplicaciones de la Tecnología Nuclear. División Bioquímica Nuclear; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Saiselet, Manuel. Université Libre de Bruxelles; Bélgica
Fil: Pisarev, Mario Alberto. Comisión Nacional de Energía Atómica. Gerencia de Área de Aplicaciones de la Tecnología Nuclear. División Bioquímica Nuclear; Argentina. Universidad de Buenos Aires. Facultad de Medicina. Departamento de Bioquímica Humana; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Fusco, Alfredo. Università degli Studi di Napoli Federico II; Italia
Fil: Juvenal, Guillermo Juan. Comisión Nacional de Energía Atómica. Gerencia de Área de Aplicaciones de la Tecnología Nuclear. División Bioquímica Nuclear; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
description It is well known that pituitary TSH exerts the major task in the regulation of thyroid function. However, this gland is capable of certain degree of autonomy, independently of TSH control. Iodine plays an important role in thyroid physiology and biochemistry. The thyroid is capable of producing different iodolipids such as 2-iodohexadecanal (2-IHDA). It was shown that this iodolipid mimic some of the inhibitory effects of excess iodide on several thyroid parameters. Objectives: To identify the miRNAs regulated by 2-IHDA in rat thyroid cells and likely characterize their role in thyroid cell proliferation and function. Results: FRTL-5 cells were grown in the presence of TSH and treated with 2-IHDA. Among the miRNAs up-regulated by 2-IHDA we focused on miR-let-7f and miR-138. When we transfected the miRNAs, miR-let-7f but not miR-138 overexpression inhibited proliferation of FRTL 5 cells, while miR-let-7f inhibition restored cell growth in 2-IHDA treated cultures. Analysis of cell cycle by flow cytometric DNA analysis revealed that miR-let-7f inhibition reduced the percentage of 2-IHDA treated cells in G1 phase and an increased of the percentage of cells in S phase was observed upon anti-let-7f transfection. The expresion of Cyclin D1 and Cyclin D3 were reduced after the transfection of miR-let-7f and miR-138, respectively. In in vivo studies we observed that miR-let-7f and miR-138 were up regulated by 2-IHDA during goiter involution. Conclusion: These results suggest that the inhibitory effects of 2-IHDA on FRTL-5 thyroid cell proliferation are mediated in part through the induction of let-7f microRNA.
publishDate 2015
dc.date.none.fl_str_mv 2015-10
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/42259
Salvarredi, Leonardo Andres; Thomasz, Lisa; Rossich, Luciano Esteban; Saiselet, Manuel; Pisarev, Mario Alberto; et al.; 2-iodohexadecanal Inhibits thyroid cell growth in part through the induction of let-7f microRNA; Elsevier Ireland; Molecular and Cellular Endocrinology; 414; 10-2015; 224-232
0303-7207
CONICET Digital
CONICET
url http://hdl.handle.net/11336/42259
identifier_str_mv Salvarredi, Leonardo Andres; Thomasz, Lisa; Rossich, Luciano Esteban; Saiselet, Manuel; Pisarev, Mario Alberto; et al.; 2-iodohexadecanal Inhibits thyroid cell growth in part through the induction of let-7f microRNA; Elsevier Ireland; Molecular and Cellular Endocrinology; 414; 10-2015; 224-232
0303-7207
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1016/j.mce.2015.07.007
info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S0303720715300162
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Elsevier Ireland
publisher.none.fl_str_mv Elsevier Ireland
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.144186

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