Multiple RNA recognition patterns during microRNA biogenesis in plants

Autores
Bologna, Nicolas Gerardo; Schapire, Arnaldo Luis; Zhai, Jixian; Chorostecki, Uciel Pablo; Boisbouvier, Jerome; Meyers, Blake C.; Palatnik, Javier Fernando
Año de publicación
2013
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
MicroRNAs (miRNAs) derive from longer precursors with fold-back structures. While animal miRNA precursors have homogenous structures, plant precursors comprise a collection of fold-backs with variable size and shape. Here, we design an approach to systematically analyze miRNA processing intermediates and characterize the biogenesis of most of the evolutionarily conserved miRNAs present in Arabidopsis thaliana. We found that plant miRNAs are processed by four mechanisms, depending on the sequential direction of the processing machinery and the number of cuts required to release the miRNA. Classification of the precursors according to their processing mechanism revealed specific structural determinants for each group. We found that the complexity of the miRNA processing pathways occurs in both ancient and evolutionarily young sequences and that members of the same family can be processed in different ways. We observed that different structural determinants compete for the processing machinery and that alternative miRNAs can be generated from a single precursor. The results provide an explanation for the structural diversity of miRNA precursors in plants and new insights toward the understanding of the biogenesis of small RNAs.
Fil: Bologna, Nicolas Gerardo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Rosario. Instituto de Biología Molecular y Celular de Rosario; Argentina
Fil: Schapire, Arnaldo Luis. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Rosario. Instituto de Biología Molecular y Celular de Rosario; Argentina
Fil: Zhai, Jixian. University of Delaware. Department of Plant & Soil Sciences; Estados Unidos. Delaware Biotechnology Institute; Estados Unidos
Fil: Chorostecki, Uciel Pablo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Rosario. Instituto de Biología Molecular y Celular de Rosario; Argentina
Fil: Boisbouvier, Jerome. Institut de Biologie Structurale Jean-Pierre Ebel; Francia
Fil: Meyers, Blake C.. University of Delaware. Department of Plant & Soil Sciences; Estados Unidos. Delaware Biotechnology Institute; Estados Unidos
Fil: Palatnik, Javier Fernando. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Rosario. Instituto de Biología Molecular y Celular de Rosario; Argentina
Materia
BIOGENESIS
MicroRNAs
Plants
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/4838
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/4838
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Multiple RNA recognition patterns during microRNA biogenesis in plantsBologna, Nicolas GerardoSchapire, Arnaldo LuisZhai, JixianChorostecki, Uciel PabloBoisbouvier, JeromeMeyers, Blake C.Palatnik, Javier FernandoBIOGENESISMicroRNAsPlantshttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1MicroRNAs (miRNAs) derive from longer precursors with fold-back structures. While animal miRNA precursors have homogenous structures, plant precursors comprise a collection of fold-backs with variable size and shape. Here, we design an approach to systematically analyze miRNA processing intermediates and characterize the biogenesis of most of the evolutionarily conserved miRNAs present in Arabidopsis thaliana. We found that plant miRNAs are processed by four mechanisms, depending on the sequential direction of the processing machinery and the number of cuts required to release the miRNA. Classification of the precursors according to their processing mechanism revealed specific structural determinants for each group. We found that the complexity of the miRNA processing pathways occurs in both ancient and evolutionarily young sequences and that members of the same family can be processed in different ways. We observed that different structural determinants compete for the processing machinery and that alternative miRNAs can be generated from a single precursor. The results provide an explanation for the structural diversity of miRNA precursors in plants and new insights toward the understanding of the biogenesis of small RNAs.Fil: Bologna, Nicolas Gerardo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Rosario. Instituto de Biología Molecular y Celular de Rosario; ArgentinaFil: Schapire, Arnaldo Luis. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Rosario. Instituto de Biología Molecular y Celular de Rosario; ArgentinaFil: Zhai, Jixian. University of Delaware. Department of Plant & Soil Sciences; Estados Unidos. Delaware Biotechnology Institute; Estados UnidosFil: Chorostecki, Uciel Pablo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Rosario. Instituto de Biología Molecular y Celular de Rosario; ArgentinaFil: Boisbouvier, Jerome. Institut de Biologie Structurale Jean-Pierre Ebel; FranciaFil: Meyers, Blake C.. University of Delaware. Department of Plant & Soil Sciences; Estados Unidos. Delaware Biotechnology Institute; Estados UnidosFil: Palatnik, Javier Fernando. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Rosario. Instituto de Biología Molecular y Celular de Rosario; ArgentinaCold Spring Harbor Lab Press2013-10info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/4838Bologna, Nicolas Gerardo; Schapire, Arnaldo Luis; Zhai, Jixian; Chorostecki, Uciel Pablo; Boisbouvier, Jerome; et al.; Multiple RNA recognition patterns during microRNA biogenesis in plants; Cold Spring Harbor Lab Press; Genome Research; 23; 10-2013; 1675-16891088-9051enginfo:eu-repo/semantics/altIdentifier/url/http://genome.cshlp.org/content/23/10/1675.longinfo:eu-repo/semantics/altIdentifier/doi/10.1101/gr.153387.112info:eu-repo/semantics/altIdentifier/doi/info:eu-repo/semantics/altIdentifier/url/http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3787264/info:eu-repo/semantics/altIdentifier/pmid/PMC3787264info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T09:52:31Zoai:ri.conicet.gov.ar:11336/4838instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 09:52:31.815CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Multiple RNA recognition patterns during microRNA biogenesis in plants
title Multiple RNA recognition patterns during microRNA biogenesis in plants
spellingShingle Multiple RNA recognition patterns during microRNA biogenesis in plants
Bologna, Nicolas Gerardo
BIOGENESIS
MicroRNAs
Plants
title_short Multiple RNA recognition patterns during microRNA biogenesis in plants
title_full Multiple RNA recognition patterns during microRNA biogenesis in plants
title_fullStr Multiple RNA recognition patterns during microRNA biogenesis in plants
title_full_unstemmed Multiple RNA recognition patterns during microRNA biogenesis in plants
title_sort Multiple RNA recognition patterns during microRNA biogenesis in plants
dc.creator.none.fl_str_mv Bologna, Nicolas Gerardo
Schapire, Arnaldo Luis
Zhai, Jixian
Chorostecki, Uciel Pablo
Boisbouvier, Jerome
Meyers, Blake C.
Palatnik, Javier Fernando
author Bologna, Nicolas Gerardo
author_facet Bologna, Nicolas Gerardo
Schapire, Arnaldo Luis
Zhai, Jixian
Chorostecki, Uciel Pablo
Boisbouvier, Jerome
Meyers, Blake C.
Palatnik, Javier Fernando
author_role author
author2 Schapire, Arnaldo Luis
Zhai, Jixian
Chorostecki, Uciel Pablo
Boisbouvier, Jerome
Meyers, Blake C.
Palatnik, Javier Fernando
author2_role author
author
author
author
author
author
dc.subject.none.fl_str_mv BIOGENESIS
MicroRNAs
Plants
topic BIOGENESIS
MicroRNAs
Plants
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv MicroRNAs (miRNAs) derive from longer precursors with fold-back structures. While animal miRNA precursors have homogenous structures, plant precursors comprise a collection of fold-backs with variable size and shape. Here, we design an approach to systematically analyze miRNA processing intermediates and characterize the biogenesis of most of the evolutionarily conserved miRNAs present in Arabidopsis thaliana. We found that plant miRNAs are processed by four mechanisms, depending on the sequential direction of the processing machinery and the number of cuts required to release the miRNA. Classification of the precursors according to their processing mechanism revealed specific structural determinants for each group. We found that the complexity of the miRNA processing pathways occurs in both ancient and evolutionarily young sequences and that members of the same family can be processed in different ways. We observed that different structural determinants compete for the processing machinery and that alternative miRNAs can be generated from a single precursor. The results provide an explanation for the structural diversity of miRNA precursors in plants and new insights toward the understanding of the biogenesis of small RNAs.
Fil: Bologna, Nicolas Gerardo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Rosario. Instituto de Biología Molecular y Celular de Rosario; Argentina
Fil: Schapire, Arnaldo Luis. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Rosario. Instituto de Biología Molecular y Celular de Rosario; Argentina
Fil: Zhai, Jixian. University of Delaware. Department of Plant & Soil Sciences; Estados Unidos. Delaware Biotechnology Institute; Estados Unidos
Fil: Chorostecki, Uciel Pablo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Rosario. Instituto de Biología Molecular y Celular de Rosario; Argentina
Fil: Boisbouvier, Jerome. Institut de Biologie Structurale Jean-Pierre Ebel; Francia
Fil: Meyers, Blake C.. University of Delaware. Department of Plant & Soil Sciences; Estados Unidos. Delaware Biotechnology Institute; Estados Unidos
Fil: Palatnik, Javier Fernando. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Rosario. Instituto de Biología Molecular y Celular de Rosario; Argentina
description MicroRNAs (miRNAs) derive from longer precursors with fold-back structures. While animal miRNA precursors have homogenous structures, plant precursors comprise a collection of fold-backs with variable size and shape. Here, we design an approach to systematically analyze miRNA processing intermediates and characterize the biogenesis of most of the evolutionarily conserved miRNAs present in Arabidopsis thaliana. We found that plant miRNAs are processed by four mechanisms, depending on the sequential direction of the processing machinery and the number of cuts required to release the miRNA. Classification of the precursors according to their processing mechanism revealed specific structural determinants for each group. We found that the complexity of the miRNA processing pathways occurs in both ancient and evolutionarily young sequences and that members of the same family can be processed in different ways. We observed that different structural determinants compete for the processing machinery and that alternative miRNAs can be generated from a single precursor. The results provide an explanation for the structural diversity of miRNA precursors in plants and new insights toward the understanding of the biogenesis of small RNAs.
publishDate 2013
dc.date.none.fl_str_mv 2013-10
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/4838
Bologna, Nicolas Gerardo; Schapire, Arnaldo Luis; Zhai, Jixian; Chorostecki, Uciel Pablo; Boisbouvier, Jerome; et al.; Multiple RNA recognition patterns during microRNA biogenesis in plants; Cold Spring Harbor Lab Press; Genome Research; 23; 10-2013; 1675-1689
1088-9051
url http://hdl.handle.net/11336/4838
identifier_str_mv Bologna, Nicolas Gerardo; Schapire, Arnaldo Luis; Zhai, Jixian; Chorostecki, Uciel Pablo; Boisbouvier, Jerome; et al.; Multiple RNA recognition patterns during microRNA biogenesis in plants; Cold Spring Harbor Lab Press; Genome Research; 23; 10-2013; 1675-1689
1088-9051
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/http://genome.cshlp.org/content/23/10/1675.long
info:eu-repo/semantics/altIdentifier/doi/10.1101/gr.153387.112
info:eu-repo/semantics/altIdentifier/doi/
info:eu-repo/semantics/altIdentifier/url/http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3787264/
info:eu-repo/semantics/altIdentifier/pmid/PMC3787264
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Cold Spring Harbor Lab Press
publisher.none.fl_str_mv Cold Spring Harbor Lab Press
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1842269165055901696
score 13.13397

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