Phytostabilization of polluted military soil supported by bioaugmentation with PGP-trace element tolerant bacteria isolated from helianthus petiolaris

Autores
Saran, Anabel; Imperato, Valeria; Fernandez, Lucia; Gkorezis, Panos; d´Haen, Jan; Merini, Luciano Jose; Vangronsveld, Jaco; Thijs, Sofie
Año de publicación
2020
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Lead (Pb) and cadmium (Cd) are major environmental pollutants, and the accumulation of these elements in soils and plants is of great concern in agricultural production due to their toxic effects on crop growth. Also, these elements can enter into the food chain and severely affect human and animal health. Bioaugmentation with plant growth-promoting bacteria (PGPB) can contribute to an environmentally friendly and effective remediation approach by improving plant survival and promoting element phytostabilization or extraction under such harsh conditions. We isolated and characterised Pb and Cd-tolerant root-associated bacteria from Helianthus petiolaris growing on a Pb/Cd polluted soil in order to compose inoculants that can promote plant growth and also ameliorate the phytostabilization or phytoextraction efficiency. One hundred and five trace element-tolerant rhizospheric and endophytic bacterial strains belonging to eight different genera were isolated from the aromatic plant species Helianthus petiolaris. Most of the strains showed multiple PGP-capabilities, ability to immobilise trace elements on their cell wall, and promotion of seed germination. Bacillus paramycoides ST9, Bacillus wiedmannii ST29, Bacillus proteolyticus ST89, Brevibacterium frigoritolerans ST30, Cellulosimicrobium cellulans ST54 and Methylobacterium sp. ST85 were selected to perform bioaugmentation assays in greenhouse microcosms. After 2 months, seedlings of sunflower (H. annuus) grown on polluted soil and inoculated with B. proteolyticus ST89 produced 40% more biomass compared to the non-inoculated control plants and accumulated 20 % less Pb and 40% less Cd in the aboveground plant parts. In contrast, B. paramycoides ST9 increased the bioaccumulation factor (BAF) of Pb three times and of Cd six times without inhibiting plant growth. Our results indicate that, depending on the strain, bioaugmentation with specific beneficial bacteria can improve plant growth and either reduce trace element mobility or enhance plant trace element uptake.
Fil: Saran, Anabel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis; Argentina. Instituto Nacional de Tecnología Agropecuaria. Centro Regional La Pampa-San Luis. Estación Experimental Agropecuaria Anguil; Argentina. Hasselt University; Bélgica
Fil: Imperato, Valeria. Hasselt University; Bélgica
Fil: Fernandez, Lucia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis; Argentina. Instituto Nacional de Tecnología Agropecuaria. Centro Regional La Pampa-San Luis. Estación Experimental Agropecuaria Anguil; Argentina
Fil: Gkorezis, Panos. Hasselt University; Bélgica
Fil: d´Haen, Jan. Hasselt University; Bélgica
Fil: Merini, Luciano Jose. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis; Argentina. Instituto Nacional de Tecnología Agropecuaria. Centro Regional La Pampa-San Luis. Estación Experimental Agropecuaria Anguil; Argentina
Fil: Vangronsveld, Jaco. Hasselt University; Bélgica. Maria Curie Sklodowska University; Polonia
Fil: Thijs, Sofie. Hasselt University; Bélgica
Materia
BIOAUGMENTATION
PB AND CD
PHYTOSTABILIZATION
PLANT GROWTH PROMOTING BACTERIA (PGPB)
POLLUTED SOIL
TRACE ELEMENTS
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/168586
Acceder
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repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Phytostabilization of polluted military soil supported by bioaugmentation with PGP-trace element tolerant bacteria isolated from helianthus petiolarisSaran, AnabelImperato, ValeriaFernandez, LuciaGkorezis, Panosd´Haen, JanMerini, Luciano JoseVangronsveld, JacoThijs, SofieBIOAUGMENTATIONPB AND CDPHYTOSTABILIZATIONPLANT GROWTH PROMOTING BACTERIA (PGPB)POLLUTED SOILTRACE ELEMENTShttps://purl.org/becyt/ford/2.8https://purl.org/becyt/ford/2Lead (Pb) and cadmium (Cd) are major environmental pollutants, and the accumulation of these elements in soils and plants is of great concern in agricultural production due to their toxic effects on crop growth. Also, these elements can enter into the food chain and severely affect human and animal health. Bioaugmentation with plant growth-promoting bacteria (PGPB) can contribute to an environmentally friendly and effective remediation approach by improving plant survival and promoting element phytostabilization or extraction under such harsh conditions. We isolated and characterised Pb and Cd-tolerant root-associated bacteria from Helianthus petiolaris growing on a Pb/Cd polluted soil in order to compose inoculants that can promote plant growth and also ameliorate the phytostabilization or phytoextraction efficiency. One hundred and five trace element-tolerant rhizospheric and endophytic bacterial strains belonging to eight different genera were isolated from the aromatic plant species Helianthus petiolaris. Most of the strains showed multiple PGP-capabilities, ability to immobilise trace elements on their cell wall, and promotion of seed germination. Bacillus paramycoides ST9, Bacillus wiedmannii ST29, Bacillus proteolyticus ST89, Brevibacterium frigoritolerans ST30, Cellulosimicrobium cellulans ST54 and Methylobacterium sp. ST85 were selected to perform bioaugmentation assays in greenhouse microcosms. After 2 months, seedlings of sunflower (H. annuus) grown on polluted soil and inoculated with B. proteolyticus ST89 produced 40% more biomass compared to the non-inoculated control plants and accumulated 20 % less Pb and 40% less Cd in the aboveground plant parts. In contrast, B. paramycoides ST9 increased the bioaccumulation factor (BAF) of Pb three times and of Cd six times without inhibiting plant growth. Our results indicate that, depending on the strain, bioaugmentation with specific beneficial bacteria can improve plant growth and either reduce trace element mobility or enhance plant trace element uptake.Fil: Saran, Anabel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis; Argentina. Instituto Nacional de Tecnología Agropecuaria. Centro Regional La Pampa-San Luis. Estación Experimental Agropecuaria Anguil; Argentina. Hasselt University; BélgicaFil: Imperato, Valeria. Hasselt University; BélgicaFil: Fernandez, Lucia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis; Argentina. Instituto Nacional de Tecnología Agropecuaria. Centro Regional La Pampa-San Luis. Estación Experimental Agropecuaria Anguil; ArgentinaFil: Gkorezis, Panos. Hasselt University; BélgicaFil: d´Haen, Jan. Hasselt University; BélgicaFil: Merini, Luciano Jose. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis; Argentina. Instituto Nacional de Tecnología Agropecuaria. Centro Regional La Pampa-San Luis. Estación Experimental Agropecuaria Anguil; ArgentinaFil: Vangronsveld, Jaco. Hasselt University; Bélgica. Maria Curie Sklodowska University; PoloniaFil: Thijs, Sofie. Hasselt University; BélgicaMultidisciplinary Digital Publishing Institute2020-02info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/168586Saran, Anabel; Imperato, Valeria; Fernandez, Lucia; Gkorezis, Panos; d´Haen, Jan; et al.; Phytostabilization of polluted military soil supported by bioaugmentation with PGP-trace element tolerant bacteria isolated from helianthus petiolaris; Multidisciplinary Digital Publishing Institute; Agronomy; 10; 2; 2-2020; 1-162073-4395CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.mdpi.com/2073-4395/10/2/204info:eu-repo/semantics/altIdentifier/doi/10.3390/agronomy10020204info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T09:57:44Zoai:ri.conicet.gov.ar:11336/168586instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 09:57:44.712CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Phytostabilization of polluted military soil supported by bioaugmentation with PGP-trace element tolerant bacteria isolated from helianthus petiolaris
title Phytostabilization of polluted military soil supported by bioaugmentation with PGP-trace element tolerant bacteria isolated from helianthus petiolaris
spellingShingle Phytostabilization of polluted military soil supported by bioaugmentation with PGP-trace element tolerant bacteria isolated from helianthus petiolaris
Saran, Anabel
BIOAUGMENTATION
PB AND CD
PHYTOSTABILIZATION
PLANT GROWTH PROMOTING BACTERIA (PGPB)
POLLUTED SOIL
TRACE ELEMENTS
title_short Phytostabilization of polluted military soil supported by bioaugmentation with PGP-trace element tolerant bacteria isolated from helianthus petiolaris
title_full Phytostabilization of polluted military soil supported by bioaugmentation with PGP-trace element tolerant bacteria isolated from helianthus petiolaris
title_fullStr Phytostabilization of polluted military soil supported by bioaugmentation with PGP-trace element tolerant bacteria isolated from helianthus petiolaris
title_full_unstemmed Phytostabilization of polluted military soil supported by bioaugmentation with PGP-trace element tolerant bacteria isolated from helianthus petiolaris
title_sort Phytostabilization of polluted military soil supported by bioaugmentation with PGP-trace element tolerant bacteria isolated from helianthus petiolaris
dc.creator.none.fl_str_mv Saran, Anabel
Imperato, Valeria
Fernandez, Lucia
Gkorezis, Panos
d´Haen, Jan
Merini, Luciano Jose
Vangronsveld, Jaco
Thijs, Sofie
author Saran, Anabel
author_facet Saran, Anabel
Imperato, Valeria
Fernandez, Lucia
Gkorezis, Panos
d´Haen, Jan
Merini, Luciano Jose
Vangronsveld, Jaco
Thijs, Sofie
author_role author
author2 Imperato, Valeria
Fernandez, Lucia
Gkorezis, Panos
d´Haen, Jan
Merini, Luciano Jose
Vangronsveld, Jaco
Thijs, Sofie
author2_role author
author
author
author
author
author
author
dc.subject.none.fl_str_mv BIOAUGMENTATION
PB AND CD
PHYTOSTABILIZATION
PLANT GROWTH PROMOTING BACTERIA (PGPB)
POLLUTED SOIL
TRACE ELEMENTS
topic BIOAUGMENTATION
PB AND CD
PHYTOSTABILIZATION
PLANT GROWTH PROMOTING BACTERIA (PGPB)
POLLUTED SOIL
TRACE ELEMENTS
purl_subject.fl_str_mv https://purl.org/becyt/ford/2.8
https://purl.org/becyt/ford/2
dc.description.none.fl_txt_mv Lead (Pb) and cadmium (Cd) are major environmental pollutants, and the accumulation of these elements in soils and plants is of great concern in agricultural production due to their toxic effects on crop growth. Also, these elements can enter into the food chain and severely affect human and animal health. Bioaugmentation with plant growth-promoting bacteria (PGPB) can contribute to an environmentally friendly and effective remediation approach by improving plant survival and promoting element phytostabilization or extraction under such harsh conditions. We isolated and characterised Pb and Cd-tolerant root-associated bacteria from Helianthus petiolaris growing on a Pb/Cd polluted soil in order to compose inoculants that can promote plant growth and also ameliorate the phytostabilization or phytoextraction efficiency. One hundred and five trace element-tolerant rhizospheric and endophytic bacterial strains belonging to eight different genera were isolated from the aromatic plant species Helianthus petiolaris. Most of the strains showed multiple PGP-capabilities, ability to immobilise trace elements on their cell wall, and promotion of seed germination. Bacillus paramycoides ST9, Bacillus wiedmannii ST29, Bacillus proteolyticus ST89, Brevibacterium frigoritolerans ST30, Cellulosimicrobium cellulans ST54 and Methylobacterium sp. ST85 were selected to perform bioaugmentation assays in greenhouse microcosms. After 2 months, seedlings of sunflower (H. annuus) grown on polluted soil and inoculated with B. proteolyticus ST89 produced 40% more biomass compared to the non-inoculated control plants and accumulated 20 % less Pb and 40% less Cd in the aboveground plant parts. In contrast, B. paramycoides ST9 increased the bioaccumulation factor (BAF) of Pb three times and of Cd six times without inhibiting plant growth. Our results indicate that, depending on the strain, bioaugmentation with specific beneficial bacteria can improve plant growth and either reduce trace element mobility or enhance plant trace element uptake.
Fil: Saran, Anabel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis; Argentina. Instituto Nacional de Tecnología Agropecuaria. Centro Regional La Pampa-San Luis. Estación Experimental Agropecuaria Anguil; Argentina. Hasselt University; Bélgica
Fil: Imperato, Valeria. Hasselt University; Bélgica
Fil: Fernandez, Lucia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis; Argentina. Instituto Nacional de Tecnología Agropecuaria. Centro Regional La Pampa-San Luis. Estación Experimental Agropecuaria Anguil; Argentina
Fil: Gkorezis, Panos. Hasselt University; Bélgica
Fil: d´Haen, Jan. Hasselt University; Bélgica
Fil: Merini, Luciano Jose. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis; Argentina. Instituto Nacional de Tecnología Agropecuaria. Centro Regional La Pampa-San Luis. Estación Experimental Agropecuaria Anguil; Argentina
Fil: Vangronsveld, Jaco. Hasselt University; Bélgica. Maria Curie Sklodowska University; Polonia
Fil: Thijs, Sofie. Hasselt University; Bélgica
description Lead (Pb) and cadmium (Cd) are major environmental pollutants, and the accumulation of these elements in soils and plants is of great concern in agricultural production due to their toxic effects on crop growth. Also, these elements can enter into the food chain and severely affect human and animal health. Bioaugmentation with plant growth-promoting bacteria (PGPB) can contribute to an environmentally friendly and effective remediation approach by improving plant survival and promoting element phytostabilization or extraction under such harsh conditions. We isolated and characterised Pb and Cd-tolerant root-associated bacteria from Helianthus petiolaris growing on a Pb/Cd polluted soil in order to compose inoculants that can promote plant growth and also ameliorate the phytostabilization or phytoextraction efficiency. One hundred and five trace element-tolerant rhizospheric and endophytic bacterial strains belonging to eight different genera were isolated from the aromatic plant species Helianthus petiolaris. Most of the strains showed multiple PGP-capabilities, ability to immobilise trace elements on their cell wall, and promotion of seed germination. Bacillus paramycoides ST9, Bacillus wiedmannii ST29, Bacillus proteolyticus ST89, Brevibacterium frigoritolerans ST30, Cellulosimicrobium cellulans ST54 and Methylobacterium sp. ST85 were selected to perform bioaugmentation assays in greenhouse microcosms. After 2 months, seedlings of sunflower (H. annuus) grown on polluted soil and inoculated with B. proteolyticus ST89 produced 40% more biomass compared to the non-inoculated control plants and accumulated 20 % less Pb and 40% less Cd in the aboveground plant parts. In contrast, B. paramycoides ST9 increased the bioaccumulation factor (BAF) of Pb three times and of Cd six times without inhibiting plant growth. Our results indicate that, depending on the strain, bioaugmentation with specific beneficial bacteria can improve plant growth and either reduce trace element mobility or enhance plant trace element uptake.
publishDate 2020
dc.date.none.fl_str_mv 2020-02
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/168586
Saran, Anabel; Imperato, Valeria; Fernandez, Lucia; Gkorezis, Panos; d´Haen, Jan; et al.; Phytostabilization of polluted military soil supported by bioaugmentation with PGP-trace element tolerant bacteria isolated from helianthus petiolaris; Multidisciplinary Digital Publishing Institute; Agronomy; 10; 2; 2-2020; 1-16
2073-4395
CONICET Digital
CONICET
url http://hdl.handle.net/11336/168586
identifier_str_mv Saran, Anabel; Imperato, Valeria; Fernandez, Lucia; Gkorezis, Panos; d´Haen, Jan; et al.; Phytostabilization of polluted military soil supported by bioaugmentation with PGP-trace element tolerant bacteria isolated from helianthus petiolaris; Multidisciplinary Digital Publishing Institute; Agronomy; 10; 2; 2-2020; 1-16
2073-4395
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://www.mdpi.com/2073-4395/10/2/204
info:eu-repo/semantics/altIdentifier/doi/10.3390/agronomy10020204
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Multidisciplinary Digital Publishing Institute
publisher.none.fl_str_mv Multidisciplinary Digital Publishing Institute
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.070432

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