Bioaugmentation of biomixtures with actinobacteria for atrazine removal: optimization of inoculum concentration

Autores
González, Samanta Katherina; Maldonado, Lourdes María; Benimeli, Claudia Susana; Polti, Marta Alejandra; Sáez, Juliana María
Año de publicación
2022
Idioma
inglés
Tipo de recurso
documento de conferencia
Estado
versión publicada
Descripción
Biopurification systems (BPS) or biobeds are bioprophylaxis systems to prevent pesticide point-source contamination, whose efficiency relies mainly on the pesticide removal capacity of the biomixture, the majority component of a BPS. The microbial metabolic abilities of the biomixture could be improved through bioaugmentation with microorganisms with specific degrading capacities, like actinobacteria. In this sense, Streptomyces sp. M7 is a previously selected actinobacterium with well-known pesticide-degrading abilities.The aim of this work was to optimize the concentration of Streptomyces sp. M7 (M7) inoculated in organic biomixtures for atrazine (ATZ) removal. For this purpose, the biomixtures B1 and B2 were formulated with soil, peat, and sugarcane bagasse and filter cake, respectively, inoculated with three concentrations of M7 (2, 4, and 8 g kg-1), and contaminated with atrazine (50 mg kg-1). The residual concentration of ATZ and different microbial groups were determined along a 28 d-assay.In general terms, at the end of the assay, an increasing trend was shown in the microbial developments of the different groups studied in both contaminated and bioaugmented biomixtures for the three concentrations of inoculum used. In B1, the microbial counts were significantly higher with 8 g kg-1 inoculum, with respect to lower inoculum concentrations: total heterotrophic microorganisms, total bacteria, fungi, and actinobacteria reached counts of 3.2 x 107; 2.7 x 107; 1.7 x 105, and 1.5 x 107 CFU g-1, respectively. In B2, total heterotrophs, total bacteria, and actinobacteria were significantly higher for the 4 g kg-1 inoculum concentration, reaching 7.9 x 107; 1.1 x 108, and 5.9 x 107 CFU g-1, respectively; fungal counts did not show significant differences. ATZ removal showed no significant differences in B1 and B2 between the three concentrations of M7 inoculum evaluated. The concentration of 2 g kg-1 of M7 was selected for further studies considering lower costs and optimum removal efficiency.
Fil: González, Samanta Katherina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina
Fil: Maldonado, Lourdes María. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina
Fil: Benimeli, Claudia Susana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina. Universidad Nacional de Catamarca; Argentina
Fil: Polti, Marta Alejandra. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina. Universidad Nacional de Tucumán. Facultad de Ciencias Naturales e Instituto Miguel Lillo; Argentina
Fil: Sáez, Juliana María. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina. Universidad Nacional de Tucumán. Facultad de Ciencias Naturales e Instituto Miguel Lillo; Argentina
XL Reunión Anual de la Sociedad de Biología de Cuyo
Mendoza
Argentina
Sociedad de Biología de Cuyo
Materia
Bioaugmentation
Biomixture
Actinobacteria
Atrazine
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/210387
Acceder
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network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Bioaugmentation of biomixtures with actinobacteria for atrazine removal: optimization of inoculum concentrationGonzález, Samanta KatherinaMaldonado, Lourdes MaríaBenimeli, Claudia SusanaPolti, Marta AlejandraSáez, Juliana MaríaBioaugmentationBiomixtureActinobacteriaAtrazinehttps://purl.org/becyt/ford/2.8https://purl.org/becyt/ford/2Biopurification systems (BPS) or biobeds are bioprophylaxis systems to prevent pesticide point-source contamination, whose efficiency relies mainly on the pesticide removal capacity of the biomixture, the majority component of a BPS. The microbial metabolic abilities of the biomixture could be improved through bioaugmentation with microorganisms with specific degrading capacities, like actinobacteria. In this sense, Streptomyces sp. M7 is a previously selected actinobacterium with well-known pesticide-degrading abilities.The aim of this work was to optimize the concentration of Streptomyces sp. M7 (M7) inoculated in organic biomixtures for atrazine (ATZ) removal. For this purpose, the biomixtures B1 and B2 were formulated with soil, peat, and sugarcane bagasse and filter cake, respectively, inoculated with three concentrations of M7 (2, 4, and 8 g kg-1), and contaminated with atrazine (50 mg kg-1). The residual concentration of ATZ and different microbial groups were determined along a 28 d-assay.In general terms, at the end of the assay, an increasing trend was shown in the microbial developments of the different groups studied in both contaminated and bioaugmented biomixtures for the three concentrations of inoculum used. In B1, the microbial counts were significantly higher with 8 g kg-1 inoculum, with respect to lower inoculum concentrations: total heterotrophic microorganisms, total bacteria, fungi, and actinobacteria reached counts of 3.2 x 107; 2.7 x 107; 1.7 x 105, and 1.5 x 107 CFU g-1, respectively. In B2, total heterotrophs, total bacteria, and actinobacteria were significantly higher for the 4 g kg-1 inoculum concentration, reaching 7.9 x 107; 1.1 x 108, and 5.9 x 107 CFU g-1, respectively; fungal counts did not show significant differences. ATZ removal showed no significant differences in B1 and B2 between the three concentrations of M7 inoculum evaluated. The concentration of 2 g kg-1 of M7 was selected for further studies considering lower costs and optimum removal efficiency.Fil: González, Samanta Katherina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; ArgentinaFil: Maldonado, Lourdes María. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; ArgentinaFil: Benimeli, Claudia Susana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina. Universidad Nacional de Catamarca; ArgentinaFil: Polti, Marta Alejandra. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina. Universidad Nacional de Tucumán. Facultad de Ciencias Naturales e Instituto Miguel Lillo; ArgentinaFil: Sáez, Juliana María. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina. Universidad Nacional de Tucumán. Facultad de Ciencias Naturales e Instituto Miguel Lillo; ArgentinaXL Reunión Anual de la Sociedad de Biología de CuyoMendozaArgentinaSociedad de Biología de CuyoSociedad de Biología de Cuyo2022info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/conferenceObjectReuniónBookhttp://purl.org/coar/resource_type/c_5794info:ar-repo/semantics/documentoDeConferenciaapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/210387Bioaugmentation of biomixtures with actinobacteria for atrazine removal: optimization of inoculum concentration; XL Reunión Anual de la Sociedad de Biología de Cuyo; Mendoza; Argentina; 2022; 87-87CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://sbcuyo.org.ar/reuniones-anuales-anteriores/Nacionalinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T10:08:31Zoai:ri.conicet.gov.ar:11336/210387instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 10:08:32.095CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Bioaugmentation of biomixtures with actinobacteria for atrazine removal: optimization of inoculum concentration
title Bioaugmentation of biomixtures with actinobacteria for atrazine removal: optimization of inoculum concentration
spellingShingle Bioaugmentation of biomixtures with actinobacteria for atrazine removal: optimization of inoculum concentration
González, Samanta Katherina
Bioaugmentation
Biomixture
Actinobacteria
Atrazine
title_short Bioaugmentation of biomixtures with actinobacteria for atrazine removal: optimization of inoculum concentration
title_full Bioaugmentation of biomixtures with actinobacteria for atrazine removal: optimization of inoculum concentration
title_fullStr Bioaugmentation of biomixtures with actinobacteria for atrazine removal: optimization of inoculum concentration
title_full_unstemmed Bioaugmentation of biomixtures with actinobacteria for atrazine removal: optimization of inoculum concentration
title_sort Bioaugmentation of biomixtures with actinobacteria for atrazine removal: optimization of inoculum concentration
dc.creator.none.fl_str_mv González, Samanta Katherina
Maldonado, Lourdes María
Benimeli, Claudia Susana
Polti, Marta Alejandra
Sáez, Juliana María
author González, Samanta Katherina
author_facet González, Samanta Katherina
Maldonado, Lourdes María
Benimeli, Claudia Susana
Polti, Marta Alejandra
Sáez, Juliana María
author_role author
author2 Maldonado, Lourdes María
Benimeli, Claudia Susana
Polti, Marta Alejandra
Sáez, Juliana María
author2_role author
author
author
author
dc.subject.none.fl_str_mv Bioaugmentation
Biomixture
Actinobacteria
Atrazine
topic Bioaugmentation
Biomixture
Actinobacteria
Atrazine
purl_subject.fl_str_mv https://purl.org/becyt/ford/2.8
https://purl.org/becyt/ford/2
dc.description.none.fl_txt_mv Biopurification systems (BPS) or biobeds are bioprophylaxis systems to prevent pesticide point-source contamination, whose efficiency relies mainly on the pesticide removal capacity of the biomixture, the majority component of a BPS. The microbial metabolic abilities of the biomixture could be improved through bioaugmentation with microorganisms with specific degrading capacities, like actinobacteria. In this sense, Streptomyces sp. M7 is a previously selected actinobacterium with well-known pesticide-degrading abilities.The aim of this work was to optimize the concentration of Streptomyces sp. M7 (M7) inoculated in organic biomixtures for atrazine (ATZ) removal. For this purpose, the biomixtures B1 and B2 were formulated with soil, peat, and sugarcane bagasse and filter cake, respectively, inoculated with three concentrations of M7 (2, 4, and 8 g kg-1), and contaminated with atrazine (50 mg kg-1). The residual concentration of ATZ and different microbial groups were determined along a 28 d-assay.In general terms, at the end of the assay, an increasing trend was shown in the microbial developments of the different groups studied in both contaminated and bioaugmented biomixtures for the three concentrations of inoculum used. In B1, the microbial counts were significantly higher with 8 g kg-1 inoculum, with respect to lower inoculum concentrations: total heterotrophic microorganisms, total bacteria, fungi, and actinobacteria reached counts of 3.2 x 107; 2.7 x 107; 1.7 x 105, and 1.5 x 107 CFU g-1, respectively. In B2, total heterotrophs, total bacteria, and actinobacteria were significantly higher for the 4 g kg-1 inoculum concentration, reaching 7.9 x 107; 1.1 x 108, and 5.9 x 107 CFU g-1, respectively; fungal counts did not show significant differences. ATZ removal showed no significant differences in B1 and B2 between the three concentrations of M7 inoculum evaluated. The concentration of 2 g kg-1 of M7 was selected for further studies considering lower costs and optimum removal efficiency.
Fil: González, Samanta Katherina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina
Fil: Maldonado, Lourdes María. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina
Fil: Benimeli, Claudia Susana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina. Universidad Nacional de Catamarca; Argentina
Fil: Polti, Marta Alejandra. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina. Universidad Nacional de Tucumán. Facultad de Ciencias Naturales e Instituto Miguel Lillo; Argentina
Fil: Sáez, Juliana María. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina. Universidad Nacional de Tucumán. Facultad de Ciencias Naturales e Instituto Miguel Lillo; Argentina
XL Reunión Anual de la Sociedad de Biología de Cuyo
Mendoza
Argentina
Sociedad de Biología de Cuyo
description Biopurification systems (BPS) or biobeds are bioprophylaxis systems to prevent pesticide point-source contamination, whose efficiency relies mainly on the pesticide removal capacity of the biomixture, the majority component of a BPS. The microbial metabolic abilities of the biomixture could be improved through bioaugmentation with microorganisms with specific degrading capacities, like actinobacteria. In this sense, Streptomyces sp. M7 is a previously selected actinobacterium with well-known pesticide-degrading abilities.The aim of this work was to optimize the concentration of Streptomyces sp. M7 (M7) inoculated in organic biomixtures for atrazine (ATZ) removal. For this purpose, the biomixtures B1 and B2 were formulated with soil, peat, and sugarcane bagasse and filter cake, respectively, inoculated with three concentrations of M7 (2, 4, and 8 g kg-1), and contaminated with atrazine (50 mg kg-1). The residual concentration of ATZ and different microbial groups were determined along a 28 d-assay.In general terms, at the end of the assay, an increasing trend was shown in the microbial developments of the different groups studied in both contaminated and bioaugmented biomixtures for the three concentrations of inoculum used. In B1, the microbial counts were significantly higher with 8 g kg-1 inoculum, with respect to lower inoculum concentrations: total heterotrophic microorganisms, total bacteria, fungi, and actinobacteria reached counts of 3.2 x 107; 2.7 x 107; 1.7 x 105, and 1.5 x 107 CFU g-1, respectively. In B2, total heterotrophs, total bacteria, and actinobacteria were significantly higher for the 4 g kg-1 inoculum concentration, reaching 7.9 x 107; 1.1 x 108, and 5.9 x 107 CFU g-1, respectively; fungal counts did not show significant differences. ATZ removal showed no significant differences in B1 and B2 between the three concentrations of M7 inoculum evaluated. The concentration of 2 g kg-1 of M7 was selected for further studies considering lower costs and optimum removal efficiency.
publishDate 2022
dc.date.none.fl_str_mv 2022
dc.type.none.fl_str_mv info:eu-repo/semantics/publishedVersion
info:eu-repo/semantics/conferenceObject
Reunión
Book
http://purl.org/coar/resource_type/c_5794
info:ar-repo/semantics/documentoDeConferencia
status_str publishedVersion
format conferenceObject
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/210387
Bioaugmentation of biomixtures with actinobacteria for atrazine removal: optimization of inoculum concentration; XL Reunión Anual de la Sociedad de Biología de Cuyo; Mendoza; Argentina; 2022; 87-87
CONICET Digital
CONICET
url http://hdl.handle.net/11336/210387
identifier_str_mv Bioaugmentation of biomixtures with actinobacteria for atrazine removal: optimization of inoculum concentration; XL Reunión Anual de la Sociedad de Biología de Cuyo; Mendoza; Argentina; 2022; 87-87
CONICET Digital
CONICET
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language eng
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https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
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dc.publisher.none.fl_str_mv Sociedad de Biología de Cuyo
publisher.none.fl_str_mv Sociedad de Biología de Cuyo
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
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