Utilization of amino acids and dipeptides by Lactobacillus plantarum from orange in nutritionally stressed conditions

Autores
Saguir de Zucal, Fabiana Maria; Loto Campos, Iris Eleonora; Manca de Nadra, M. C.
Año de publicación
2008
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
To investigate amino acid and dipeptide utilization by Lactobacillus plantarum N4 isolated from orange peel, in a nutritionally depleted medium basedon MRS (Mann, Rogosa, Sharpe).Methods and Results: In MRS with 0 Æ1 g l)1 of meat extract and without peptone and yeast extract, growth increased when essential and stimulatory amino acids and nonessential amino acid were added to the medium. Replacement of the essential amino acid, leucine, and the nonessential amino acid, glycine, byleucyl-leucine (Leu-Leu) and ⁄or glycyl-glycine (Gly-Gly) significantly enhancedgrowth. Essential amino acids were mainly consumed and the dipeptides were almost completely used at the end of growth. Leucine and glycine accumulatedinternally from the peptides were higher than from the free amino acids.Glucose utilization increased in the media containing dipeptides comparedwith the medium containing free amino acids.Conclusions: In a N-depleted medium, Leu-Leu and ⁄or Gly-Gly were more effective than the respective amino acids in supporting growth of the microorganism. The more efficient internal accumulation of glycine and especially leucine from dipeptides confirmed the ability of the strain to assimilate mainly complex nitrogen molecules rather than simple ones.Significance and Impact of the Study: The ability of Lact. plantarum N4 to efficiently use dipeptides could contribute to spoilage development in the natural medium of the organism, orange juice
Fil: Saguir de Zucal, Fabiana Maria. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia. Instituto de Microbiología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán; Argentina
Fil: Loto Campos, Iris Eleonora. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia. Instituto de Microbiología; Argentina
Fil: Manca de Nadra, M. C.. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia. Instituto de Microbiología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina
Materia
Amino Acids And Peptides
Lactobacillus Plantarum
Metabolism
Nutrition
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/82129
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/82129
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Utilization of amino acids and dipeptides by Lactobacillus plantarum from orange in nutritionally stressed conditionsSaguir de Zucal, Fabiana MariaLoto Campos, Iris EleonoraManca de Nadra, M. C.Amino Acids And PeptidesLactobacillus PlantarumMetabolismNutritionhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1To investigate amino acid and dipeptide utilization by Lactobacillus plantarum N4 isolated from orange peel, in a nutritionally depleted medium basedon MRS (Mann, Rogosa, Sharpe).Methods and Results: In MRS with 0 Æ1 g l)1 of meat extract and without peptone and yeast extract, growth increased when essential and stimulatory amino acids and nonessential amino acid were added to the medium. Replacement of the essential amino acid, leucine, and the nonessential amino acid, glycine, byleucyl-leucine (Leu-Leu) and ⁄or glycyl-glycine (Gly-Gly) significantly enhancedgrowth. Essential amino acids were mainly consumed and the dipeptides were almost completely used at the end of growth. Leucine and glycine accumulatedinternally from the peptides were higher than from the free amino acids.Glucose utilization increased in the media containing dipeptides comparedwith the medium containing free amino acids.Conclusions: In a N-depleted medium, Leu-Leu and ⁄or Gly-Gly were more effective than the respective amino acids in supporting growth of the microorganism. The more efficient internal accumulation of glycine and especially leucine from dipeptides confirmed the ability of the strain to assimilate mainly complex nitrogen molecules rather than simple ones.Significance and Impact of the Study: The ability of Lact. plantarum N4 to efficiently use dipeptides could contribute to spoilage development in the natural medium of the organism, orange juiceFil: Saguir de Zucal, Fabiana Maria. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia. Instituto de Microbiología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán; ArgentinaFil: Loto Campos, Iris Eleonora. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia. Instituto de Microbiología; ArgentinaFil: Manca de Nadra, M. C.. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia. Instituto de Microbiología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; ArgentinaWiley Blackwell Publishing, Inc2008-12info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/82129Saguir de Zucal, Fabiana Maria; Loto Campos, Iris Eleonora; Manca de Nadra, M. C.; Utilization of amino acids and dipeptides by Lactobacillus plantarum from orange in nutritionally stressed conditions; Wiley Blackwell Publishing, Inc; Journal of Applied Microbiology; 104; 6; 12-2008; 1597-16041364-5072CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1111/j.1365-2672.2007.03708.xinfo:eu-repo/semantics/altIdentifier/url/https://onlinelibrary.wiley.com/doi/epdf/10.1111/j.1365-2672.2007.03708.xinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T09:43:23Zoai:ri.conicet.gov.ar:11336/82129instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 09:43:24.1CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Utilization of amino acids and dipeptides by Lactobacillus plantarum from orange in nutritionally stressed conditions
title Utilization of amino acids and dipeptides by Lactobacillus plantarum from orange in nutritionally stressed conditions
spellingShingle Utilization of amino acids and dipeptides by Lactobacillus plantarum from orange in nutritionally stressed conditions
Saguir de Zucal, Fabiana Maria
Amino Acids And Peptides
Lactobacillus Plantarum
Metabolism
Nutrition
title_short Utilization of amino acids and dipeptides by Lactobacillus plantarum from orange in nutritionally stressed conditions
title_full Utilization of amino acids and dipeptides by Lactobacillus plantarum from orange in nutritionally stressed conditions
title_fullStr Utilization of amino acids and dipeptides by Lactobacillus plantarum from orange in nutritionally stressed conditions
title_full_unstemmed Utilization of amino acids and dipeptides by Lactobacillus plantarum from orange in nutritionally stressed conditions
title_sort Utilization of amino acids and dipeptides by Lactobacillus plantarum from orange in nutritionally stressed conditions
dc.creator.none.fl_str_mv Saguir de Zucal, Fabiana Maria
Loto Campos, Iris Eleonora
Manca de Nadra, M. C.
author Saguir de Zucal, Fabiana Maria
author_facet Saguir de Zucal, Fabiana Maria
Loto Campos, Iris Eleonora
Manca de Nadra, M. C.
author_role author
author2 Loto Campos, Iris Eleonora
Manca de Nadra, M. C.
author2_role author
author
dc.subject.none.fl_str_mv Amino Acids And Peptides
Lactobacillus Plantarum
Metabolism
Nutrition
topic Amino Acids And Peptides
Lactobacillus Plantarum
Metabolism
Nutrition
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv To investigate amino acid and dipeptide utilization by Lactobacillus plantarum N4 isolated from orange peel, in a nutritionally depleted medium basedon MRS (Mann, Rogosa, Sharpe).Methods and Results: In MRS with 0 Æ1 g l)1 of meat extract and without peptone and yeast extract, growth increased when essential and stimulatory amino acids and nonessential amino acid were added to the medium. Replacement of the essential amino acid, leucine, and the nonessential amino acid, glycine, byleucyl-leucine (Leu-Leu) and ⁄or glycyl-glycine (Gly-Gly) significantly enhancedgrowth. Essential amino acids were mainly consumed and the dipeptides were almost completely used at the end of growth. Leucine and glycine accumulatedinternally from the peptides were higher than from the free amino acids.Glucose utilization increased in the media containing dipeptides comparedwith the medium containing free amino acids.Conclusions: In a N-depleted medium, Leu-Leu and ⁄or Gly-Gly were more effective than the respective amino acids in supporting growth of the microorganism. The more efficient internal accumulation of glycine and especially leucine from dipeptides confirmed the ability of the strain to assimilate mainly complex nitrogen molecules rather than simple ones.Significance and Impact of the Study: The ability of Lact. plantarum N4 to efficiently use dipeptides could contribute to spoilage development in the natural medium of the organism, orange juice
Fil: Saguir de Zucal, Fabiana Maria. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia. Instituto de Microbiología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán; Argentina
Fil: Loto Campos, Iris Eleonora. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia. Instituto de Microbiología; Argentina
Fil: Manca de Nadra, M. C.. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia. Instituto de Microbiología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina
description To investigate amino acid and dipeptide utilization by Lactobacillus plantarum N4 isolated from orange peel, in a nutritionally depleted medium basedon MRS (Mann, Rogosa, Sharpe).Methods and Results: In MRS with 0 Æ1 g l)1 of meat extract and without peptone and yeast extract, growth increased when essential and stimulatory amino acids and nonessential amino acid were added to the medium. Replacement of the essential amino acid, leucine, and the nonessential amino acid, glycine, byleucyl-leucine (Leu-Leu) and ⁄or glycyl-glycine (Gly-Gly) significantly enhancedgrowth. Essential amino acids were mainly consumed and the dipeptides were almost completely used at the end of growth. Leucine and glycine accumulatedinternally from the peptides were higher than from the free amino acids.Glucose utilization increased in the media containing dipeptides comparedwith the medium containing free amino acids.Conclusions: In a N-depleted medium, Leu-Leu and ⁄or Gly-Gly were more effective than the respective amino acids in supporting growth of the microorganism. The more efficient internal accumulation of glycine and especially leucine from dipeptides confirmed the ability of the strain to assimilate mainly complex nitrogen molecules rather than simple ones.Significance and Impact of the Study: The ability of Lact. plantarum N4 to efficiently use dipeptides could contribute to spoilage development in the natural medium of the organism, orange juice
publishDate 2008
dc.date.none.fl_str_mv 2008-12
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/82129
Saguir de Zucal, Fabiana Maria; Loto Campos, Iris Eleonora; Manca de Nadra, M. C.; Utilization of amino acids and dipeptides by Lactobacillus plantarum from orange in nutritionally stressed conditions; Wiley Blackwell Publishing, Inc; Journal of Applied Microbiology; 104; 6; 12-2008; 1597-1604
1364-5072
CONICET Digital
CONICET
url http://hdl.handle.net/11336/82129
identifier_str_mv Saguir de Zucal, Fabiana Maria; Loto Campos, Iris Eleonora; Manca de Nadra, M. C.; Utilization of amino acids and dipeptides by Lactobacillus plantarum from orange in nutritionally stressed conditions; Wiley Blackwell Publishing, Inc; Journal of Applied Microbiology; 104; 6; 12-2008; 1597-1604
1364-5072
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1111/j.1365-2672.2007.03708.x
info:eu-repo/semantics/altIdentifier/url/https://onlinelibrary.wiley.com/doi/epdf/10.1111/j.1365-2672.2007.03708.x
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Wiley Blackwell Publishing, Inc
publisher.none.fl_str_mv Wiley Blackwell Publishing, Inc
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.070432

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