Loss of Sertoli-Germ Cell Adhesion Determines the Rapid Germ Cell Elimination During the Seasonal Regression of the Seminiferous Epithelium of the Long Hairy Armadillo Chaetophract...

Autores
Luaces, Juan Pablo; Rossi, Luis Francisco; Sciurano, Roberta Beatriz; Rebuzzini, Paola; Merico, Valeria; Zuccotti, Maurizio; Merani, Maria Susana; Garagna, Silvia
Año de publicación
2014
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The armadillo Chaetophractus villosus is a seasonal breeder whose seminiferous epithelium undergoes rapid regression with massive germ cell loss, leaving the tubules with only Sertoli cells and spermatogonia. Here, we addressed the question of whether this regression entails 1) the disassembly of cell junctions (immunolocalization of nectin-3, Cadm1, N-cadherin, and beta-catenin, and transmission electron microscopy [TEM]); 2) apoptosis (immunolocalization of cytochrome c and caspase 3; TUNEL assay); and 3) the involvement of Sertoli cells in germ cell phagocytosis (TEM). We showed a dramatic reduction in the extension of vimentin filaments associated with desmosomelike junctions at the interface between Sertoli and germ cells, and an increased diffusion of the immunosignals of nectin-3, Cadm1, N-cadherin, and beta-catenin. Together, these results suggest loss of Sertoli-germ cell adhesion, which in turn might determine postmeiotic cell sloughing at the beginning of epithelium regression. Then, loss of Sertoli-germ cell adhesion triggers cell death. Cytochrome c is released from mitochondria, but although postmeiotic cells were negative for late apoptotic markers, at advanced regression spermatocytes were positive for all apoptotic markers. Transmission electron microscopy analysis showed cytoplasmic engulfment of cell debris and lipid droplets within Sertoli cells, a sign of their phagocytic activity, which contributes to the elimination of the residual meiocytes still present in the latest regression phases. These findings are novel and add new players to the mechanisms of seminiferous epithelium regression occurring in seasonal breeders, and they introduce the armadillo as an interesting model for studying seasonal spermatogenesis.
Fil: Luaces, Juan Pablo. Universidad de Buenos Aires. Facultad de Medicina. Laboratorio de Biología Cromosómica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Rossi, Luis Francisco. Universidad de Buenos Aires. Facultad de Medicina. Laboratorio de Biología Cromosómica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Sciurano, Roberta Beatriz. Universidad de Buenos Aires. Facultad de Medicina. Laboratorio de Biología Cromosómica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Rebuzzini, Paola. Università degli Studi di Pavia; Italia
Fil: Merico, Valeria. Università degli Studi di Pavia; Italia
Fil: Zuccotti, Maurizio. Università di Parma; Italia
Fil: Merani, Maria Susana. Universidad de Buenos Aires. Facultad de Medicina. Laboratorio de Biología Cromosómica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Garagna, Silvia. Università degli Studi di Pavia; Italia
Materia
Adhesion Molecules
Anoikis
Apoptosis
Cadm1
Intermediate Filaments
N-Cadherin
Β-Catenin
Nectin-3
Phagocytosis
Seasonal Reproduction
Spermatogenesis
Testosterone
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/16985
Acceder
id CONICETDig_eca2586d9ad624839fc395ebe661ca53
oai_identifier_str oai:ri.conicet.gov.ar:11336/16985
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Loss of Sertoli-Germ Cell Adhesion Determines the Rapid Germ Cell Elimination During the Seasonal Regression of the Seminiferous Epithelium of the Long Hairy Armadillo Chaetophractus villosusLuaces, Juan PabloRossi, Luis FranciscoSciurano, Roberta BeatrizRebuzzini, PaolaMerico, ValeriaZuccotti, MaurizioMerani, Maria SusanaGaragna, SilviaAdhesion MoleculesAnoikisApoptosisCadm1Intermediate FilamentsN-CadherinΒ-CateninNectin-3PhagocytosisSeasonal ReproductionSpermatogenesisTestosteronehttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1https://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1The armadillo Chaetophractus villosus is a seasonal breeder whose seminiferous epithelium undergoes rapid regression with massive germ cell loss, leaving the tubules with only Sertoli cells and spermatogonia. Here, we addressed the question of whether this regression entails 1) the disassembly of cell junctions (immunolocalization of nectin-3, Cadm1, N-cadherin, and beta-catenin, and transmission electron microscopy [TEM]); 2) apoptosis (immunolocalization of cytochrome c and caspase 3; TUNEL assay); and 3) the involvement of Sertoli cells in germ cell phagocytosis (TEM). We showed a dramatic reduction in the extension of vimentin filaments associated with desmosomelike junctions at the interface between Sertoli and germ cells, and an increased diffusion of the immunosignals of nectin-3, Cadm1, N-cadherin, and beta-catenin. Together, these results suggest loss of Sertoli-germ cell adhesion, which in turn might determine postmeiotic cell sloughing at the beginning of epithelium regression. Then, loss of Sertoli-germ cell adhesion triggers cell death. Cytochrome c is released from mitochondria, but although postmeiotic cells were negative for late apoptotic markers, at advanced regression spermatocytes were positive for all apoptotic markers. Transmission electron microscopy analysis showed cytoplasmic engulfment of cell debris and lipid droplets within Sertoli cells, a sign of their phagocytic activity, which contributes to the elimination of the residual meiocytes still present in the latest regression phases. These findings are novel and add new players to the mechanisms of seminiferous epithelium regression occurring in seasonal breeders, and they introduce the armadillo as an interesting model for studying seasonal spermatogenesis.Fil: Luaces, Juan Pablo. Universidad de Buenos Aires. Facultad de Medicina. Laboratorio de Biología Cromosómica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Rossi, Luis Francisco. Universidad de Buenos Aires. Facultad de Medicina. Laboratorio de Biología Cromosómica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Sciurano, Roberta Beatriz. Universidad de Buenos Aires. Facultad de Medicina. Laboratorio de Biología Cromosómica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Rebuzzini, Paola. Università degli Studi di Pavia; ItaliaFil: Merico, Valeria. Università degli Studi di Pavia; ItaliaFil: Zuccotti, Maurizio. Università di Parma; ItaliaFil: Merani, Maria Susana. Universidad de Buenos Aires. Facultad de Medicina. Laboratorio de Biología Cromosómica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Garagna, Silvia. Università degli Studi di Pavia; ItaliaOxford University Press2014-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/16985Luaces, Juan Pablo; Rossi, Luis Francisco; Sciurano, Roberta Beatriz; Rebuzzini, Paola; Merico, Valeria; et al.; Loss of Sertoli-Germ Cell Adhesion Determines the Rapid Germ Cell Elimination During the Seasonal Regression of the Seminiferous Epithelium of the Long Hairy Armadillo Chaetophractus villosus; Oxford University Press; Biology of Reproduction; 90; 48; 1-2014; 1-110006-33631529-7268enginfo:eu-repo/semantics/altIdentifier/url/https://academic.oup.com/biolreprod/article-lookup/doi/10.1095/biolreprod.113.113118info:eu-repo/semantics/altIdentifier/doi/10.1095/biolreprod.113.113118info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-11-05T09:39:45Zoai:ri.conicet.gov.ar:11336/16985instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-11-05 09:39:45.318CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Loss of Sertoli-Germ Cell Adhesion Determines the Rapid Germ Cell Elimination During the Seasonal Regression of the Seminiferous Epithelium of the Long Hairy Armadillo Chaetophractus villosus
title Loss of Sertoli-Germ Cell Adhesion Determines the Rapid Germ Cell Elimination During the Seasonal Regression of the Seminiferous Epithelium of the Long Hairy Armadillo Chaetophractus villosus
spellingShingle Loss of Sertoli-Germ Cell Adhesion Determines the Rapid Germ Cell Elimination During the Seasonal Regression of the Seminiferous Epithelium of the Long Hairy Armadillo Chaetophractus villosus
Luaces, Juan Pablo
Adhesion Molecules
Anoikis
Apoptosis
Cadm1
Intermediate Filaments
N-Cadherin
Β-Catenin
Nectin-3
Phagocytosis
Seasonal Reproduction
Spermatogenesis
Testosterone
title_short Loss of Sertoli-Germ Cell Adhesion Determines the Rapid Germ Cell Elimination During the Seasonal Regression of the Seminiferous Epithelium of the Long Hairy Armadillo Chaetophractus villosus
title_full Loss of Sertoli-Germ Cell Adhesion Determines the Rapid Germ Cell Elimination During the Seasonal Regression of the Seminiferous Epithelium of the Long Hairy Armadillo Chaetophractus villosus
title_fullStr Loss of Sertoli-Germ Cell Adhesion Determines the Rapid Germ Cell Elimination During the Seasonal Regression of the Seminiferous Epithelium of the Long Hairy Armadillo Chaetophractus villosus
title_full_unstemmed Loss of Sertoli-Germ Cell Adhesion Determines the Rapid Germ Cell Elimination During the Seasonal Regression of the Seminiferous Epithelium of the Long Hairy Armadillo Chaetophractus villosus
title_sort Loss of Sertoli-Germ Cell Adhesion Determines the Rapid Germ Cell Elimination During the Seasonal Regression of the Seminiferous Epithelium of the Long Hairy Armadillo Chaetophractus villosus
dc.creator.none.fl_str_mv Luaces, Juan Pablo
Rossi, Luis Francisco
Sciurano, Roberta Beatriz
Rebuzzini, Paola
Merico, Valeria
Zuccotti, Maurizio
Merani, Maria Susana
Garagna, Silvia
author Luaces, Juan Pablo
author_facet Luaces, Juan Pablo
Rossi, Luis Francisco
Sciurano, Roberta Beatriz
Rebuzzini, Paola
Merico, Valeria
Zuccotti, Maurizio
Merani, Maria Susana
Garagna, Silvia
author_role author
author2 Rossi, Luis Francisco
Sciurano, Roberta Beatriz
Rebuzzini, Paola
Merico, Valeria
Zuccotti, Maurizio
Merani, Maria Susana
Garagna, Silvia
author2_role author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Adhesion Molecules
Anoikis
Apoptosis
Cadm1
Intermediate Filaments
N-Cadherin
Β-Catenin
Nectin-3
Phagocytosis
Seasonal Reproduction
Spermatogenesis
Testosterone
topic Adhesion Molecules
Anoikis
Apoptosis
Cadm1
Intermediate Filaments
N-Cadherin
Β-Catenin
Nectin-3
Phagocytosis
Seasonal Reproduction
Spermatogenesis
Testosterone
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv The armadillo Chaetophractus villosus is a seasonal breeder whose seminiferous epithelium undergoes rapid regression with massive germ cell loss, leaving the tubules with only Sertoli cells and spermatogonia. Here, we addressed the question of whether this regression entails 1) the disassembly of cell junctions (immunolocalization of nectin-3, Cadm1, N-cadherin, and beta-catenin, and transmission electron microscopy [TEM]); 2) apoptosis (immunolocalization of cytochrome c and caspase 3; TUNEL assay); and 3) the involvement of Sertoli cells in germ cell phagocytosis (TEM). We showed a dramatic reduction in the extension of vimentin filaments associated with desmosomelike junctions at the interface between Sertoli and germ cells, and an increased diffusion of the immunosignals of nectin-3, Cadm1, N-cadherin, and beta-catenin. Together, these results suggest loss of Sertoli-germ cell adhesion, which in turn might determine postmeiotic cell sloughing at the beginning of epithelium regression. Then, loss of Sertoli-germ cell adhesion triggers cell death. Cytochrome c is released from mitochondria, but although postmeiotic cells were negative for late apoptotic markers, at advanced regression spermatocytes were positive for all apoptotic markers. Transmission electron microscopy analysis showed cytoplasmic engulfment of cell debris and lipid droplets within Sertoli cells, a sign of their phagocytic activity, which contributes to the elimination of the residual meiocytes still present in the latest regression phases. These findings are novel and add new players to the mechanisms of seminiferous epithelium regression occurring in seasonal breeders, and they introduce the armadillo as an interesting model for studying seasonal spermatogenesis.
Fil: Luaces, Juan Pablo. Universidad de Buenos Aires. Facultad de Medicina. Laboratorio de Biología Cromosómica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Rossi, Luis Francisco. Universidad de Buenos Aires. Facultad de Medicina. Laboratorio de Biología Cromosómica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Sciurano, Roberta Beatriz. Universidad de Buenos Aires. Facultad de Medicina. Laboratorio de Biología Cromosómica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Rebuzzini, Paola. Università degli Studi di Pavia; Italia
Fil: Merico, Valeria. Università degli Studi di Pavia; Italia
Fil: Zuccotti, Maurizio. Università di Parma; Italia
Fil: Merani, Maria Susana. Universidad de Buenos Aires. Facultad de Medicina. Laboratorio de Biología Cromosómica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Garagna, Silvia. Università degli Studi di Pavia; Italia
description The armadillo Chaetophractus villosus is a seasonal breeder whose seminiferous epithelium undergoes rapid regression with massive germ cell loss, leaving the tubules with only Sertoli cells and spermatogonia. Here, we addressed the question of whether this regression entails 1) the disassembly of cell junctions (immunolocalization of nectin-3, Cadm1, N-cadherin, and beta-catenin, and transmission electron microscopy [TEM]); 2) apoptosis (immunolocalization of cytochrome c and caspase 3; TUNEL assay); and 3) the involvement of Sertoli cells in germ cell phagocytosis (TEM). We showed a dramatic reduction in the extension of vimentin filaments associated with desmosomelike junctions at the interface between Sertoli and germ cells, and an increased diffusion of the immunosignals of nectin-3, Cadm1, N-cadherin, and beta-catenin. Together, these results suggest loss of Sertoli-germ cell adhesion, which in turn might determine postmeiotic cell sloughing at the beginning of epithelium regression. Then, loss of Sertoli-germ cell adhesion triggers cell death. Cytochrome c is released from mitochondria, but although postmeiotic cells were negative for late apoptotic markers, at advanced regression spermatocytes were positive for all apoptotic markers. Transmission electron microscopy analysis showed cytoplasmic engulfment of cell debris and lipid droplets within Sertoli cells, a sign of their phagocytic activity, which contributes to the elimination of the residual meiocytes still present in the latest regression phases. These findings are novel and add new players to the mechanisms of seminiferous epithelium regression occurring in seasonal breeders, and they introduce the armadillo as an interesting model for studying seasonal spermatogenesis.
publishDate 2014
dc.date.none.fl_str_mv 2014-01
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/16985
Luaces, Juan Pablo; Rossi, Luis Francisco; Sciurano, Roberta Beatriz; Rebuzzini, Paola; Merico, Valeria; et al.; Loss of Sertoli-Germ Cell Adhesion Determines the Rapid Germ Cell Elimination During the Seasonal Regression of the Seminiferous Epithelium of the Long Hairy Armadillo Chaetophractus villosus; Oxford University Press; Biology of Reproduction; 90; 48; 1-2014; 1-11
0006-3363
1529-7268
url http://hdl.handle.net/11336/16985
identifier_str_mv Luaces, Juan Pablo; Rossi, Luis Francisco; Sciurano, Roberta Beatriz; Rebuzzini, Paola; Merico, Valeria; et al.; Loss of Sertoli-Germ Cell Adhesion Determines the Rapid Germ Cell Elimination During the Seasonal Regression of the Seminiferous Epithelium of the Long Hairy Armadillo Chaetophractus villosus; Oxford University Press; Biology of Reproduction; 90; 48; 1-2014; 1-11
0006-3363
1529-7268
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://academic.oup.com/biolreprod/article-lookup/doi/10.1095/biolreprod.113.113118
info:eu-repo/semantics/altIdentifier/doi/10.1095/biolreprod.113.113118
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
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dc.publisher.none.fl_str_mv Oxford University Press
publisher.none.fl_str_mv Oxford University Press
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.082534

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