Role of E-cadherin in epithelial architecture maintenance

Autores
Izaguirre, María Fernanda; Larrea, D.; Adur, Javier Fernando; Diaz Zamboni, Javier Eduardo; Vicente, N. B.; Galetto, Carolina; Casco, Victor Hugo
Año de publicación
2010
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Morphogenesis and architecture of a developing epithelium is controlled by both cell shape and contacts, mediated by spatially and temporally regulated cell adhesion molecules. The authors study if E-cadherin functions as a key factor of epithelial adhesion and epidermal architecture in vivo. They apply whole-mount digital deconvolution microscopy to evaluate three-dimensional (3D) E-cadherin expression during skin morphogenesis of Rhinella arenarum and in a cell adhesion alteration model. Results show morphogenetic changes in the 3D E-cadherin spatiotemporal expression pattern correlated with the increase of E-cadherin and in the number of cells with hexagonal geometry. Alterations in junctionprotein phosphorylation showed drastic loss of E-cadherin and β-catenin in cell-cell contacts and the increase of cytoplasm and nuclear β-catenin in epidermis, suggesting the activation of the β-catenin signal pathway. Surprisingly, no changes in cell shape and skin architecture were registered, suggesting that epidermal E-cadherin appears to be involved in signaling rather than cell contact maintenance in vivo.
Fil: Izaguirre, María Fernanda. Universidad Nacional de Entre Ríos. Facultad de Ingeniería. Departamento de Biología. Laboratorio de Microscopía; Argentina
Fil: Larrea, D.. Universidad Nacional de Entre Ríos. Facultad de Ingeniería. Departamento de Biología. Laboratorio de Microscopía; Argentina
Fil: Adur, Javier Fernando. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de Entre Ríos. Facultad de Ingeniería. Departamento de Biología. Laboratorio de Microscopía; Argentina
Fil: Diaz Zamboni, Javier Eduardo. Universidad Nacional de Entre Ríos. Facultad de Ingeniería. Departamento de Biología. Laboratorio de Microscopía; Argentina
Fil: Vicente, N. B.. Universidad Nacional de Entre Ríos. Facultad de Ingeniería. Departamento de Biología. Laboratorio de Microscopía; Argentina
Fil: Galetto, Carolina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de Entre Ríos. Facultad de Ingeniería. Departamento de Biología. Laboratorio de Microscopía; Argentina
Fil: Casco, Victor Hugo. Universidad Nacional de Entre Ríos. Facultad de Ingeniería. Departamento de Biología. Laboratorio de Microscopía; Argentina
Materia
ANURAN
CADHERIN
CATENIN
OPTICAL SECTIONING MICROSCOPY
SKIN
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/186603
Acceder
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repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Role of E-cadherin in epithelial architecture maintenanceIzaguirre, María FernandaLarrea, D.Adur, Javier FernandoDiaz Zamboni, Javier EduardoVicente, N. B.Galetto, CarolinaCasco, Victor HugoANURANCADHERINCATENINOPTICAL SECTIONING MICROSCOPYSKINhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Morphogenesis and architecture of a developing epithelium is controlled by both cell shape and contacts, mediated by spatially and temporally regulated cell adhesion molecules. The authors study if E-cadherin functions as a key factor of epithelial adhesion and epidermal architecture in vivo. They apply whole-mount digital deconvolution microscopy to evaluate three-dimensional (3D) E-cadherin expression during skin morphogenesis of Rhinella arenarum and in a cell adhesion alteration model. Results show morphogenetic changes in the 3D E-cadherin spatiotemporal expression pattern correlated with the increase of E-cadherin and in the number of cells with hexagonal geometry. Alterations in junctionprotein phosphorylation showed drastic loss of E-cadherin and β-catenin in cell-cell contacts and the increase of cytoplasm and nuclear β-catenin in epidermis, suggesting the activation of the β-catenin signal pathway. Surprisingly, no changes in cell shape and skin architecture were registered, suggesting that epidermal E-cadherin appears to be involved in signaling rather than cell contact maintenance in vivo.Fil: Izaguirre, María Fernanda. Universidad Nacional de Entre Ríos. Facultad de Ingeniería. Departamento de Biología. Laboratorio de Microscopía; ArgentinaFil: Larrea, D.. Universidad Nacional de Entre Ríos. Facultad de Ingeniería. Departamento de Biología. Laboratorio de Microscopía; ArgentinaFil: Adur, Javier Fernando. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de Entre Ríos. Facultad de Ingeniería. Departamento de Biología. Laboratorio de Microscopía; ArgentinaFil: Diaz Zamboni, Javier Eduardo. Universidad Nacional de Entre Ríos. Facultad de Ingeniería. Departamento de Biología. Laboratorio de Microscopía; ArgentinaFil: Vicente, N. B.. Universidad Nacional de Entre Ríos. Facultad de Ingeniería. Departamento de Biología. Laboratorio de Microscopía; ArgentinaFil: Galetto, Carolina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de Entre Ríos. Facultad de Ingeniería. Departamento de Biología. Laboratorio de Microscopía; ArgentinaFil: Casco, Victor Hugo. Universidad Nacional de Entre Ríos. Facultad de Ingeniería. Departamento de Biología. Laboratorio de Microscopía; ArgentinaTaylor & Francis2010-03info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/186603Izaguirre, María Fernanda; Larrea, D.; Adur, Javier Fernando; Diaz Zamboni, Javier Eduardo; Vicente, N. B.; et al.; Role of E-cadherin in epithelial architecture maintenance; Taylor & Francis; Cell Communication And Adhesion; 17; 1; 3-2010; 1-121541-9061CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.tandfonline.com/doi/full/10.3109/15419061003686938info:eu-repo/semantics/altIdentifier/doi/10.3109/15419061003686938info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-10T13:22:01Zoai:ri.conicet.gov.ar:11336/186603instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-10 13:22:01.721CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Role of E-cadherin in epithelial architecture maintenance
title Role of E-cadherin in epithelial architecture maintenance
spellingShingle Role of E-cadherin in epithelial architecture maintenance
Izaguirre, María Fernanda
ANURAN
CADHERIN
CATENIN
OPTICAL SECTIONING MICROSCOPY
SKIN
title_short Role of E-cadherin in epithelial architecture maintenance
title_full Role of E-cadherin in epithelial architecture maintenance
title_fullStr Role of E-cadherin in epithelial architecture maintenance
title_full_unstemmed Role of E-cadherin in epithelial architecture maintenance
title_sort Role of E-cadherin in epithelial architecture maintenance
dc.creator.none.fl_str_mv Izaguirre, María Fernanda
Larrea, D.
Adur, Javier Fernando
Diaz Zamboni, Javier Eduardo
Vicente, N. B.
Galetto, Carolina
Casco, Victor Hugo
author Izaguirre, María Fernanda
author_facet Izaguirre, María Fernanda
Larrea, D.
Adur, Javier Fernando
Diaz Zamboni, Javier Eduardo
Vicente, N. B.
Galetto, Carolina
Casco, Victor Hugo
author_role author
author2 Larrea, D.
Adur, Javier Fernando
Diaz Zamboni, Javier Eduardo
Vicente, N. B.
Galetto, Carolina
Casco, Victor Hugo
author2_role author
author
author
author
author
author
dc.subject.none.fl_str_mv ANURAN
CADHERIN
CATENIN
OPTICAL SECTIONING MICROSCOPY
SKIN
topic ANURAN
CADHERIN
CATENIN
OPTICAL SECTIONING MICROSCOPY
SKIN
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Morphogenesis and architecture of a developing epithelium is controlled by both cell shape and contacts, mediated by spatially and temporally regulated cell adhesion molecules. The authors study if E-cadherin functions as a key factor of epithelial adhesion and epidermal architecture in vivo. They apply whole-mount digital deconvolution microscopy to evaluate three-dimensional (3D) E-cadherin expression during skin morphogenesis of Rhinella arenarum and in a cell adhesion alteration model. Results show morphogenetic changes in the 3D E-cadherin spatiotemporal expression pattern correlated with the increase of E-cadherin and in the number of cells with hexagonal geometry. Alterations in junctionprotein phosphorylation showed drastic loss of E-cadherin and β-catenin in cell-cell contacts and the increase of cytoplasm and nuclear β-catenin in epidermis, suggesting the activation of the β-catenin signal pathway. Surprisingly, no changes in cell shape and skin architecture were registered, suggesting that epidermal E-cadherin appears to be involved in signaling rather than cell contact maintenance in vivo.
Fil: Izaguirre, María Fernanda. Universidad Nacional de Entre Ríos. Facultad de Ingeniería. Departamento de Biología. Laboratorio de Microscopía; Argentina
Fil: Larrea, D.. Universidad Nacional de Entre Ríos. Facultad de Ingeniería. Departamento de Biología. Laboratorio de Microscopía; Argentina
Fil: Adur, Javier Fernando. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de Entre Ríos. Facultad de Ingeniería. Departamento de Biología. Laboratorio de Microscopía; Argentina
Fil: Diaz Zamboni, Javier Eduardo. Universidad Nacional de Entre Ríos. Facultad de Ingeniería. Departamento de Biología. Laboratorio de Microscopía; Argentina
Fil: Vicente, N. B.. Universidad Nacional de Entre Ríos. Facultad de Ingeniería. Departamento de Biología. Laboratorio de Microscopía; Argentina
Fil: Galetto, Carolina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de Entre Ríos. Facultad de Ingeniería. Departamento de Biología. Laboratorio de Microscopía; Argentina
Fil: Casco, Victor Hugo. Universidad Nacional de Entre Ríos. Facultad de Ingeniería. Departamento de Biología. Laboratorio de Microscopía; Argentina
description Morphogenesis and architecture of a developing epithelium is controlled by both cell shape and contacts, mediated by spatially and temporally regulated cell adhesion molecules. The authors study if E-cadherin functions as a key factor of epithelial adhesion and epidermal architecture in vivo. They apply whole-mount digital deconvolution microscopy to evaluate three-dimensional (3D) E-cadherin expression during skin morphogenesis of Rhinella arenarum and in a cell adhesion alteration model. Results show morphogenetic changes in the 3D E-cadherin spatiotemporal expression pattern correlated with the increase of E-cadherin and in the number of cells with hexagonal geometry. Alterations in junctionprotein phosphorylation showed drastic loss of E-cadherin and β-catenin in cell-cell contacts and the increase of cytoplasm and nuclear β-catenin in epidermis, suggesting the activation of the β-catenin signal pathway. Surprisingly, no changes in cell shape and skin architecture were registered, suggesting that epidermal E-cadherin appears to be involved in signaling rather than cell contact maintenance in vivo.
publishDate 2010
dc.date.none.fl_str_mv 2010-03
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/186603
Izaguirre, María Fernanda; Larrea, D.; Adur, Javier Fernando; Diaz Zamboni, Javier Eduardo; Vicente, N. B.; et al.; Role of E-cadherin in epithelial architecture maintenance; Taylor & Francis; Cell Communication And Adhesion; 17; 1; 3-2010; 1-12
1541-9061
CONICET Digital
CONICET
url http://hdl.handle.net/11336/186603
identifier_str_mv Izaguirre, María Fernanda; Larrea, D.; Adur, Javier Fernando; Diaz Zamboni, Javier Eduardo; Vicente, N. B.; et al.; Role of E-cadherin in epithelial architecture maintenance; Taylor & Francis; Cell Communication And Adhesion; 17; 1; 3-2010; 1-12
1541-9061
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://www.tandfonline.com/doi/full/10.3109/15419061003686938
info:eu-repo/semantics/altIdentifier/doi/10.3109/15419061003686938
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Taylor & Francis
publisher.none.fl_str_mv Taylor & Francis
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1842981212361064448
score 12.48226

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