M2e-Displaying Virus-Like Particles with Associated RNA Promote T Helper 1 Type Adaptive Immunity against Influenza A

Autores
Ibañez, Lorena Itatí; Roose, Kenny; De Filette, Marina; Schotsaert, Michael; De Sloovere, Jessica; Roels, Stefan; Pollard, Charlotte; Schepens, Bert; Grooten, Johan; Fiers, Walter; Saelens, Xavier
Año de publicación
2013
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The ectodomain of influenza A matrix protein 2 (M2e) is a candidate for a universal influenza A vaccine. We used recombinant Hepatitis B core antigen to produce virus-like particles presenting M2e (M2e-VLPs). We produced the VLPs with and without entrapped nucleic acids and compared their immunogenicity and protective efficacy. Immunization of BALB/c mice with M2e-VLPs containing nucleic acids induced a stronger, Th1-biased antibody response compared to particles lacking nucleic acids. The former also induced a stronger M2e-specific CD4+ T cell response, as determined by ELISPOT. Mice vaccinated with alum-adjuvanted M2e-VLPs containing the nucleic acid-binding domain were better protected against influenza A virus challenge than mice vaccinated with similar particles lacking this domain, as deduced from the loss in body weight following challenge with X47 (H3N2) or PR/8 virus. Challenge of mice that had been immunized with M2e-VLPs with or without nucleic acids displayed significantly lower mortality, morbidity and lung virus titers than control-immunized groups. We conclude that nucleic acids present in M2e-VLPs correlate with improved immune protection.
Fil: Ibañez, Lorena Itatí. University of Ghent; Bélgica. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Roose, Kenny. University of Ghent; Bélgica
Fil: De Filette, Marina. University of Ghent; Bélgica
Fil: Schotsaert, Michael. University of Ghent; Bélgica
Fil: De Sloovere, Jessica. Veterinary and Agrochemical Research Centre; Bélgica
Fil: Roels, Stefan. Veterinary and Agrochemical Research Centre; Bélgica
Fil: Pollard, Charlotte. University of Ghent; Bélgica. Institute of Tropical Medicine; Bélgica
Fil: Schepens, Bert. University of Ghent; Bélgica
Fil: Grooten, Johan. University of Ghent; Bélgica
Fil: Fiers, Walter. University of Ghent; Bélgica
Fil: Saelens, Xavier. University of Ghent; Bélgica
Materia
INFLUENZA
UNIVERSAL VACCINE
M2e
VIRUS-LIKE PARTICLE
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/19004
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/19004
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling M2e-Displaying Virus-Like Particles with Associated RNA Promote T Helper 1 Type Adaptive Immunity against Influenza AIbañez, Lorena ItatíRoose, KennyDe Filette, MarinaSchotsaert, MichaelDe Sloovere, JessicaRoels, StefanPollard, CharlotteSchepens, BertGrooten, JohanFiers, WalterSaelens, XavierINFLUENZAUNIVERSAL VACCINEM2eVIRUS-LIKE PARTICLEhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1The ectodomain of influenza A matrix protein 2 (M2e) is a candidate for a universal influenza A vaccine. We used recombinant Hepatitis B core antigen to produce virus-like particles presenting M2e (M2e-VLPs). We produced the VLPs with and without entrapped nucleic acids and compared their immunogenicity and protective efficacy. Immunization of BALB/c mice with M2e-VLPs containing nucleic acids induced a stronger, Th1-biased antibody response compared to particles lacking nucleic acids. The former also induced a stronger M2e-specific CD4+ T cell response, as determined by ELISPOT. Mice vaccinated with alum-adjuvanted M2e-VLPs containing the nucleic acid-binding domain were better protected against influenza A virus challenge than mice vaccinated with similar particles lacking this domain, as deduced from the loss in body weight following challenge with X47 (H3N2) or PR/8 virus. Challenge of mice that had been immunized with M2e-VLPs with or without nucleic acids displayed significantly lower mortality, morbidity and lung virus titers than control-immunized groups. We conclude that nucleic acids present in M2e-VLPs correlate with improved immune protection.Fil: Ibañez, Lorena Itatí. University of Ghent; Bélgica. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Roose, Kenny. University of Ghent; BélgicaFil: De Filette, Marina. University of Ghent; BélgicaFil: Schotsaert, Michael. University of Ghent; BélgicaFil: De Sloovere, Jessica. Veterinary and Agrochemical Research Centre; BélgicaFil: Roels, Stefan. Veterinary and Agrochemical Research Centre; BélgicaFil: Pollard, Charlotte. University of Ghent; Bélgica. Institute of Tropical Medicine; BélgicaFil: Schepens, Bert. University of Ghent; BélgicaFil: Grooten, Johan. University of Ghent; BélgicaFil: Fiers, Walter. University of Ghent; BélgicaFil: Saelens, Xavier. University of Ghent; BélgicaPublic Library of Science2013-03info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/19004Ibañez, Lorena Itatí; Roose, Kenny ; De Filette, Marina; Schotsaert, Michael; De Sloovere, Jessica; et al.; M2e-Displaying Virus-Like Particles with Associated RNA Promote T Helper 1 Type Adaptive Immunity against Influenza A; Public Library of Science; Plos One; 8; 3; 3-2013; 1-12; e590811932-6203CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1371/journal.pone.0059081info:eu-repo/semantics/altIdentifier/url/http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0059081info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-10T13:21:03Zoai:ri.conicet.gov.ar:11336/19004instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-10 13:21:03.789CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv M2e-Displaying Virus-Like Particles with Associated RNA Promote T Helper 1 Type Adaptive Immunity against Influenza A
title M2e-Displaying Virus-Like Particles with Associated RNA Promote T Helper 1 Type Adaptive Immunity against Influenza A
spellingShingle M2e-Displaying Virus-Like Particles with Associated RNA Promote T Helper 1 Type Adaptive Immunity against Influenza A
Ibañez, Lorena Itatí
INFLUENZA
UNIVERSAL VACCINE
M2e
VIRUS-LIKE PARTICLE
title_short M2e-Displaying Virus-Like Particles with Associated RNA Promote T Helper 1 Type Adaptive Immunity against Influenza A
title_full M2e-Displaying Virus-Like Particles with Associated RNA Promote T Helper 1 Type Adaptive Immunity against Influenza A
title_fullStr M2e-Displaying Virus-Like Particles with Associated RNA Promote T Helper 1 Type Adaptive Immunity against Influenza A
title_full_unstemmed M2e-Displaying Virus-Like Particles with Associated RNA Promote T Helper 1 Type Adaptive Immunity against Influenza A
title_sort M2e-Displaying Virus-Like Particles with Associated RNA Promote T Helper 1 Type Adaptive Immunity against Influenza A
dc.creator.none.fl_str_mv Ibañez, Lorena Itatí
Roose, Kenny
De Filette, Marina
Schotsaert, Michael
De Sloovere, Jessica
Roels, Stefan
Pollard, Charlotte
Schepens, Bert
Grooten, Johan
Fiers, Walter
Saelens, Xavier
author Ibañez, Lorena Itatí
author_facet Ibañez, Lorena Itatí
Roose, Kenny
De Filette, Marina
Schotsaert, Michael
De Sloovere, Jessica
Roels, Stefan
Pollard, Charlotte
Schepens, Bert
Grooten, Johan
Fiers, Walter
Saelens, Xavier
author_role author
author2 Roose, Kenny
De Filette, Marina
Schotsaert, Michael
De Sloovere, Jessica
Roels, Stefan
Pollard, Charlotte
Schepens, Bert
Grooten, Johan
Fiers, Walter
Saelens, Xavier
author2_role author
author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv INFLUENZA
UNIVERSAL VACCINE
M2e
VIRUS-LIKE PARTICLE
topic INFLUENZA
UNIVERSAL VACCINE
M2e
VIRUS-LIKE PARTICLE
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv The ectodomain of influenza A matrix protein 2 (M2e) is a candidate for a universal influenza A vaccine. We used recombinant Hepatitis B core antigen to produce virus-like particles presenting M2e (M2e-VLPs). We produced the VLPs with and without entrapped nucleic acids and compared their immunogenicity and protective efficacy. Immunization of BALB/c mice with M2e-VLPs containing nucleic acids induced a stronger, Th1-biased antibody response compared to particles lacking nucleic acids. The former also induced a stronger M2e-specific CD4+ T cell response, as determined by ELISPOT. Mice vaccinated with alum-adjuvanted M2e-VLPs containing the nucleic acid-binding domain were better protected against influenza A virus challenge than mice vaccinated with similar particles lacking this domain, as deduced from the loss in body weight following challenge with X47 (H3N2) or PR/8 virus. Challenge of mice that had been immunized with M2e-VLPs with or without nucleic acids displayed significantly lower mortality, morbidity and lung virus titers than control-immunized groups. We conclude that nucleic acids present in M2e-VLPs correlate with improved immune protection.
Fil: Ibañez, Lorena Itatí. University of Ghent; Bélgica. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Roose, Kenny. University of Ghent; Bélgica
Fil: De Filette, Marina. University of Ghent; Bélgica
Fil: Schotsaert, Michael. University of Ghent; Bélgica
Fil: De Sloovere, Jessica. Veterinary and Agrochemical Research Centre; Bélgica
Fil: Roels, Stefan. Veterinary and Agrochemical Research Centre; Bélgica
Fil: Pollard, Charlotte. University of Ghent; Bélgica. Institute of Tropical Medicine; Bélgica
Fil: Schepens, Bert. University of Ghent; Bélgica
Fil: Grooten, Johan. University of Ghent; Bélgica
Fil: Fiers, Walter. University of Ghent; Bélgica
Fil: Saelens, Xavier. University of Ghent; Bélgica
description The ectodomain of influenza A matrix protein 2 (M2e) is a candidate for a universal influenza A vaccine. We used recombinant Hepatitis B core antigen to produce virus-like particles presenting M2e (M2e-VLPs). We produced the VLPs with and without entrapped nucleic acids and compared their immunogenicity and protective efficacy. Immunization of BALB/c mice with M2e-VLPs containing nucleic acids induced a stronger, Th1-biased antibody response compared to particles lacking nucleic acids. The former also induced a stronger M2e-specific CD4+ T cell response, as determined by ELISPOT. Mice vaccinated with alum-adjuvanted M2e-VLPs containing the nucleic acid-binding domain were better protected against influenza A virus challenge than mice vaccinated with similar particles lacking this domain, as deduced from the loss in body weight following challenge with X47 (H3N2) or PR/8 virus. Challenge of mice that had been immunized with M2e-VLPs with or without nucleic acids displayed significantly lower mortality, morbidity and lung virus titers than control-immunized groups. We conclude that nucleic acids present in M2e-VLPs correlate with improved immune protection.
publishDate 2013
dc.date.none.fl_str_mv 2013-03
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/19004
Ibañez, Lorena Itatí; Roose, Kenny ; De Filette, Marina; Schotsaert, Michael; De Sloovere, Jessica; et al.; M2e-Displaying Virus-Like Particles with Associated RNA Promote T Helper 1 Type Adaptive Immunity against Influenza A; Public Library of Science; Plos One; 8; 3; 3-2013; 1-12; e59081
1932-6203
CONICET Digital
CONICET
url http://hdl.handle.net/11336/19004
identifier_str_mv Ibañez, Lorena Itatí; Roose, Kenny ; De Filette, Marina; Schotsaert, Michael; De Sloovere, Jessica; et al.; M2e-Displaying Virus-Like Particles with Associated RNA Promote T Helper 1 Type Adaptive Immunity against Influenza A; Public Library of Science; Plos One; 8; 3; 3-2013; 1-12; e59081
1932-6203
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1371/journal.pone.0059081
info:eu-repo/semantics/altIdentifier/url/http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0059081
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Public Library of Science
publisher.none.fl_str_mv Public Library of Science
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1842981154239545344
score 12.493442

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