Natural and long-lasting cellular immune responses against influenza in the M2e-immune host

Autores
Schotsaert, M.; Ysenbaert, T.; Neyt, K.; Ibañez, Lorena Itatí; Bogaert, P.; Schepens, B.; Lambrecht, B. N.; Fiers, W.; Saelens, X.
Año de publicación
2013
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Influenza is a global health concern. Licensed influenza vaccines induce strain-specific virus-neutralizing antibodies but hamper the induction of possibly cross-protective T-cell responses upon subsequent infection. 1 In this study, we compared protection induced by a vaccine based on the conserved extracellular domain of matrix 2 protein (M2e) with that of a conventional whole inactivated virus (WIV) vaccine using single as well as consecutive homo- and heterosubtypic challenges. Both vaccines protected against a primary homologous (with respect to hemagglutinin and neuraminidase in WIV) challenge. Functional T-cell responses were induced after primary challenge of M2e-immune mice but were absent in WIV-vaccinated mice. M2e-immune mice displayed limited inducible bronchus-associated lymphoid tissue, which was absent in WIV-immune animals. Importantly, M2e- but not WIV-immune mice were protected from a primary as well as a secondary, severe heterosubtypic challenge, including challenge with pandemic H1N1 2009 virus. Our findings advocate the use of infection-permissive influenza vaccines, such as those based on M2e, in immunologically naive individuals. The combined immune response induced by M2e-vaccine and by clinically controlled influenza virus replication results in strong and broad protection against pandemic influenza. We conclude that the challenge of the M2e-immune host induces strong and broadly reactive immunity against influenza virus infection.
Fil: Schotsaert, M.. Flanders Interuniversity Institute For Biotechnology; Bélgica. University of Ghent; Bélgica
Fil: Ysenbaert, T.. Flanders Interuniversity Institute For Biotechnology; Bélgica. University of Ghent; Bélgica
Fil: Neyt, K.. Flanders Interuniversity Institute For Biotechnology; Bélgica. University of Ghent; Bélgica
Fil: Ibañez, Lorena Itatí. Flanders Interuniversity Institute For Biotechnology; Bélgica. University of Ghent; Bélgica. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Bogaert, P.. University of Ghent; Bélgica. Flanders Interuniversity Institute For Biotechnology; Bélgica
Fil: Schepens, B.. Flanders Interuniversity Institute For Biotechnology; Bélgica. University of Ghent; Bélgica
Fil: Lambrecht, B. N.. University of Ghent; Bélgica. Flanders Interuniversity Institute For Biotechnology; Bélgica
Fil: Fiers, W.. University of Ghent; Bélgica. Flanders Interuniversity Institute For Biotechnology; Bélgica
Fil: Saelens, X.. Flanders Interuniversity Institute For Biotechnology; Bélgica. University of Ghent; Bélgica
Materia
UNIVERSAL VACCINE
CELULLAR IMMUNOLOGY
INFLUENZA
VACCINATION
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/96180
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/96180
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Natural and long-lasting cellular immune responses against influenza in the M2e-immune hostSchotsaert, M.Ysenbaert, T.Neyt, K.Ibañez, Lorena ItatíBogaert, P.Schepens, B.Lambrecht, B. N.Fiers, W.Saelens, X.UNIVERSAL VACCINECELULLAR IMMUNOLOGYINFLUENZAVACCINATIONhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1https://purl.org/becyt/ford/3.4https://purl.org/becyt/ford/3Influenza is a global health concern. Licensed influenza vaccines induce strain-specific virus-neutralizing antibodies but hamper the induction of possibly cross-protective T-cell responses upon subsequent infection. 1 In this study, we compared protection induced by a vaccine based on the conserved extracellular domain of matrix 2 protein (M2e) with that of a conventional whole inactivated virus (WIV) vaccine using single as well as consecutive homo- and heterosubtypic challenges. Both vaccines protected against a primary homologous (with respect to hemagglutinin and neuraminidase in WIV) challenge. Functional T-cell responses were induced after primary challenge of M2e-immune mice but were absent in WIV-vaccinated mice. M2e-immune mice displayed limited inducible bronchus-associated lymphoid tissue, which was absent in WIV-immune animals. Importantly, M2e- but not WIV-immune mice were protected from a primary as well as a secondary, severe heterosubtypic challenge, including challenge with pandemic H1N1 2009 virus. Our findings advocate the use of infection-permissive influenza vaccines, such as those based on M2e, in immunologically naive individuals. The combined immune response induced by M2e-vaccine and by clinically controlled influenza virus replication results in strong and broad protection against pandemic influenza. We conclude that the challenge of the M2e-immune host induces strong and broadly reactive immunity against influenza virus infection.Fil: Schotsaert, M.. Flanders Interuniversity Institute For Biotechnology; Bélgica. University of Ghent; BélgicaFil: Ysenbaert, T.. Flanders Interuniversity Institute For Biotechnology; Bélgica. University of Ghent; BélgicaFil: Neyt, K.. Flanders Interuniversity Institute For Biotechnology; Bélgica. University of Ghent; BélgicaFil: Ibañez, Lorena Itatí. Flanders Interuniversity Institute For Biotechnology; Bélgica. University of Ghent; Bélgica. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Bogaert, P.. University of Ghent; Bélgica. Flanders Interuniversity Institute For Biotechnology; BélgicaFil: Schepens, B.. Flanders Interuniversity Institute For Biotechnology; Bélgica. University of Ghent; BélgicaFil: Lambrecht, B. N.. University of Ghent; Bélgica. Flanders Interuniversity Institute For Biotechnology; BélgicaFil: Fiers, W.. University of Ghent; Bélgica. Flanders Interuniversity Institute For Biotechnology; BélgicaFil: Saelens, X.. Flanders Interuniversity Institute For Biotechnology; Bélgica. University of Ghent; BélgicaNature Publishing Group2013-03info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/96180Schotsaert, M.; Ysenbaert, T.; Neyt, K.; Ibañez, Lorena Itatí; Bogaert, P.; et al.; Natural and long-lasting cellular immune responses against influenza in the M2e-immune host; Nature Publishing Group; Mucosal Immunology; 6; 2; 3-2013; 276-2871933-0219CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.nature.com/articles/mi201269info:eu-repo/semantics/altIdentifier/doi/10.1038/mi.2012.69info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T09:44:16Zoai:ri.conicet.gov.ar:11336/96180instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 09:44:16.525CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Natural and long-lasting cellular immune responses against influenza in the M2e-immune host
title Natural and long-lasting cellular immune responses against influenza in the M2e-immune host
spellingShingle Natural and long-lasting cellular immune responses against influenza in the M2e-immune host
Schotsaert, M.
UNIVERSAL VACCINE
CELULLAR IMMUNOLOGY
INFLUENZA
VACCINATION
title_short Natural and long-lasting cellular immune responses against influenza in the M2e-immune host
title_full Natural and long-lasting cellular immune responses against influenza in the M2e-immune host
title_fullStr Natural and long-lasting cellular immune responses against influenza in the M2e-immune host
title_full_unstemmed Natural and long-lasting cellular immune responses against influenza in the M2e-immune host
title_sort Natural and long-lasting cellular immune responses against influenza in the M2e-immune host
dc.creator.none.fl_str_mv Schotsaert, M.
Ysenbaert, T.
Neyt, K.
Ibañez, Lorena Itatí
Bogaert, P.
Schepens, B.
Lambrecht, B. N.
Fiers, W.
Saelens, X.
author Schotsaert, M.
author_facet Schotsaert, M.
Ysenbaert, T.
Neyt, K.
Ibañez, Lorena Itatí
Bogaert, P.
Schepens, B.
Lambrecht, B. N.
Fiers, W.
Saelens, X.
author_role author
author2 Ysenbaert, T.
Neyt, K.
Ibañez, Lorena Itatí
Bogaert, P.
Schepens, B.
Lambrecht, B. N.
Fiers, W.
Saelens, X.
author2_role author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv UNIVERSAL VACCINE
CELULLAR IMMUNOLOGY
INFLUENZA
VACCINATION
topic UNIVERSAL VACCINE
CELULLAR IMMUNOLOGY
INFLUENZA
VACCINATION
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
https://purl.org/becyt/ford/3.4
https://purl.org/becyt/ford/3
dc.description.none.fl_txt_mv Influenza is a global health concern. Licensed influenza vaccines induce strain-specific virus-neutralizing antibodies but hamper the induction of possibly cross-protective T-cell responses upon subsequent infection. 1 In this study, we compared protection induced by a vaccine based on the conserved extracellular domain of matrix 2 protein (M2e) with that of a conventional whole inactivated virus (WIV) vaccine using single as well as consecutive homo- and heterosubtypic challenges. Both vaccines protected against a primary homologous (with respect to hemagglutinin and neuraminidase in WIV) challenge. Functional T-cell responses were induced after primary challenge of M2e-immune mice but were absent in WIV-vaccinated mice. M2e-immune mice displayed limited inducible bronchus-associated lymphoid tissue, which was absent in WIV-immune animals. Importantly, M2e- but not WIV-immune mice were protected from a primary as well as a secondary, severe heterosubtypic challenge, including challenge with pandemic H1N1 2009 virus. Our findings advocate the use of infection-permissive influenza vaccines, such as those based on M2e, in immunologically naive individuals. The combined immune response induced by M2e-vaccine and by clinically controlled influenza virus replication results in strong and broad protection against pandemic influenza. We conclude that the challenge of the M2e-immune host induces strong and broadly reactive immunity against influenza virus infection.
Fil: Schotsaert, M.. Flanders Interuniversity Institute For Biotechnology; Bélgica. University of Ghent; Bélgica
Fil: Ysenbaert, T.. Flanders Interuniversity Institute For Biotechnology; Bélgica. University of Ghent; Bélgica
Fil: Neyt, K.. Flanders Interuniversity Institute For Biotechnology; Bélgica. University of Ghent; Bélgica
Fil: Ibañez, Lorena Itatí. Flanders Interuniversity Institute For Biotechnology; Bélgica. University of Ghent; Bélgica. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Bogaert, P.. University of Ghent; Bélgica. Flanders Interuniversity Institute For Biotechnology; Bélgica
Fil: Schepens, B.. Flanders Interuniversity Institute For Biotechnology; Bélgica. University of Ghent; Bélgica
Fil: Lambrecht, B. N.. University of Ghent; Bélgica. Flanders Interuniversity Institute For Biotechnology; Bélgica
Fil: Fiers, W.. University of Ghent; Bélgica. Flanders Interuniversity Institute For Biotechnology; Bélgica
Fil: Saelens, X.. Flanders Interuniversity Institute For Biotechnology; Bélgica. University of Ghent; Bélgica
description Influenza is a global health concern. Licensed influenza vaccines induce strain-specific virus-neutralizing antibodies but hamper the induction of possibly cross-protective T-cell responses upon subsequent infection. 1 In this study, we compared protection induced by a vaccine based on the conserved extracellular domain of matrix 2 protein (M2e) with that of a conventional whole inactivated virus (WIV) vaccine using single as well as consecutive homo- and heterosubtypic challenges. Both vaccines protected against a primary homologous (with respect to hemagglutinin and neuraminidase in WIV) challenge. Functional T-cell responses were induced after primary challenge of M2e-immune mice but were absent in WIV-vaccinated mice. M2e-immune mice displayed limited inducible bronchus-associated lymphoid tissue, which was absent in WIV-immune animals. Importantly, M2e- but not WIV-immune mice were protected from a primary as well as a secondary, severe heterosubtypic challenge, including challenge with pandemic H1N1 2009 virus. Our findings advocate the use of infection-permissive influenza vaccines, such as those based on M2e, in immunologically naive individuals. The combined immune response induced by M2e-vaccine and by clinically controlled influenza virus replication results in strong and broad protection against pandemic influenza. We conclude that the challenge of the M2e-immune host induces strong and broadly reactive immunity against influenza virus infection.
publishDate 2013
dc.date.none.fl_str_mv 2013-03
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/96180
Schotsaert, M.; Ysenbaert, T.; Neyt, K.; Ibañez, Lorena Itatí; Bogaert, P.; et al.; Natural and long-lasting cellular immune responses against influenza in the M2e-immune host; Nature Publishing Group; Mucosal Immunology; 6; 2; 3-2013; 276-287
1933-0219
CONICET Digital
CONICET
url http://hdl.handle.net/11336/96180
identifier_str_mv Schotsaert, M.; Ysenbaert, T.; Neyt, K.; Ibañez, Lorena Itatí; Bogaert, P.; et al.; Natural and long-lasting cellular immune responses against influenza in the M2e-immune host; Nature Publishing Group; Mucosal Immunology; 6; 2; 3-2013; 276-287
1933-0219
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://www.nature.com/articles/mi201269
info:eu-repo/semantics/altIdentifier/doi/10.1038/mi.2012.69
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Nature Publishing Group
publisher.none.fl_str_mv Nature Publishing Group
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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