Highest diploid number among Gymnotiformes: first cytogenetic insights into Rhabdolichops (Sternopygidae)

Autores
Suarez, Pablo; Pinto Barroso, Inaê Cristina Guerreiro; Silva, Danilo dos Santos; Milhomem, Susana S. R.; Cabral de Mello, Diogo Cavalcanti; Martins, Cesar; Pieczarka, Julio Cesar; Nagamachi, Cleusa Yoshiko
Año de publicación
2017
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
We report the first comparative cytogenetic analysis of two species from electrogenic fish of genus Rhabdolichops (Sternopygidae, Gymnotiformes): Rhabdolichops troscheli and Rhabdolichops cf eastwardi. R. troscheli has 2n = 54 (fundamental number [FN] = 66), whereas R. cf. eastwardi has 2n = 74 (FN = 78). C-banding revealed centromeric constitutive heterochromatin in both species. Ag-NORs mapped on pair 6 in R. troscheli and pair 30 in R. cf eastwardi. Fluorescense in situ hybridization with 18S rDNA probes confirmed the Ag-NOR staining results and revealed additional (presumably silent) ribosomal genes on pairs 12, 13, 21, 23, 26, and 27 in R. cf eastwardi. 5S rDNA was found on the centromeres of pair 7 in both species. Telomeric probes showed only distal locations. Dispersed signal patterns were obtained using probes for retrotransposons Rex1 and Rex3. Histone H1 and H3 genes were found together on pair 6 in R. cf eastwardi. The high diploid number found in Rhabdolichops suggests that chromosome fission may have contributed to its chromosomal evolution, phylogenetic relationship of the Sternopygidae suggests that this increase in diploid number could be a synapomorphic characteristic of genus Rhabdolichops. Although both species are phylogenetically close related, their karyotype structure has undergone divergent evolutionary directions. All in all, our results strongly suggest that R. cf eastwardi experencied recent intense genome reorganization.
Fil: Suarez, Pablo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste. Instituto de Biología Subtropical. Instituto de Biología Subtropical - Nodo Puerto Iguazú | Universidad Nacional de Misiones. Instituto de Biología Subtropical. Instituto de Biología Subtropical - Nodo Puerto Iguazú; Argentina. Universidade Federal do Pará; Brasil
Fil: Pinto Barroso, Inaê Cristina Guerreiro. Universidade Federal do Pará; Brasil
Fil: Silva, Danilo dos Santos. Universidade do Estado de Pará; Brasil
Fil: Milhomem, Susana S. R.. Instituto Federal de Educação, Ciência e Tecnologia de Góias; Brasil
Fil: Cabral de Mello, Diogo Cavalcanti. Universidade Estadual Paulista Julio de Mesquita Filho; Brasil
Fil: Martins, Cesar. Universidade Estadual Paulista Julio de Mesquita Filho; Brasil
Fil: Pieczarka, Julio Cesar. Universidade Federal do Pará; Brasil
Fil: Nagamachi, Cleusa Yoshiko. Universidade Federal do Pará; Brasil
Materia
Electric Fish
Chromosomes
Biodiversity
Amazon Rainforest
Kariyotypic Diversity
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/46291
Acceder
id CONICETDig_eb46ab2a90bbddef8ff89fe50949eb66
oai_identifier_str oai:ri.conicet.gov.ar:11336/46291
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Highest diploid number among Gymnotiformes: first cytogenetic insights into Rhabdolichops (Sternopygidae)Suarez, PabloPinto Barroso, Inaê Cristina GuerreiroSilva, Danilo dos SantosMilhomem, Susana S. R.Cabral de Mello, Diogo CavalcantiMartins, CesarPieczarka, Julio CesarNagamachi, Cleusa YoshikoElectric FishChromosomesBiodiversityAmazon RainforestKariyotypic Diversityhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1We report the first comparative cytogenetic analysis of two species from electrogenic fish of genus Rhabdolichops (Sternopygidae, Gymnotiformes): Rhabdolichops troscheli and Rhabdolichops cf eastwardi. R. troscheli has 2n = 54 (fundamental number [FN] = 66), whereas R. cf. eastwardi has 2n = 74 (FN = 78). C-banding revealed centromeric constitutive heterochromatin in both species. Ag-NORs mapped on pair 6 in R. troscheli and pair 30 in R. cf eastwardi. Fluorescense in situ hybridization with 18S rDNA probes confirmed the Ag-NOR staining results and revealed additional (presumably silent) ribosomal genes on pairs 12, 13, 21, 23, 26, and 27 in R. cf eastwardi. 5S rDNA was found on the centromeres of pair 7 in both species. Telomeric probes showed only distal locations. Dispersed signal patterns were obtained using probes for retrotransposons Rex1 and Rex3. Histone H1 and H3 genes were found together on pair 6 in R. cf eastwardi. The high diploid number found in Rhabdolichops suggests that chromosome fission may have contributed to its chromosomal evolution, phylogenetic relationship of the Sternopygidae suggests that this increase in diploid number could be a synapomorphic characteristic of genus Rhabdolichops. Although both species are phylogenetically close related, their karyotype structure has undergone divergent evolutionary directions. All in all, our results strongly suggest that R. cf eastwardi experencied recent intense genome reorganization.Fil: Suarez, Pablo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste. Instituto de Biología Subtropical. Instituto de Biología Subtropical - Nodo Puerto Iguazú | Universidad Nacional de Misiones. Instituto de Biología Subtropical. Instituto de Biología Subtropical - Nodo Puerto Iguazú; Argentina. Universidade Federal do Pará; BrasilFil: Pinto Barroso, Inaê Cristina Guerreiro. Universidade Federal do Pará; BrasilFil: Silva, Danilo dos Santos. Universidade do Estado de Pará; BrasilFil: Milhomem, Susana S. R.. Instituto Federal de Educação, Ciência e Tecnologia de Góias; BrasilFil: Cabral de Mello, Diogo Cavalcanti. Universidade Estadual Paulista Julio de Mesquita Filho; BrasilFil: Martins, Cesar. Universidade Estadual Paulista Julio de Mesquita Filho; BrasilFil: Pieczarka, Julio Cesar. Universidade Federal do Pará; BrasilFil: Nagamachi, Cleusa Yoshiko. Universidade Federal do Pará; BrasilMary Ann Liebert2017-06info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/46291Suarez, Pablo; Pinto Barroso, Inaê Cristina Guerreiro; Silva, Danilo dos Santos; Milhomem, Susana S. R.; Cabral de Mello, Diogo Cavalcanti; et al.; Highest diploid number among Gymnotiformes: first cytogenetic insights into Rhabdolichops (Sternopygidae); Mary Ann Liebert; Zebrafish; 14; 3; 6-2017; 272-2791545-85471557-8542CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/http://online.liebertpub.com/doi/full/10.1089/zeb.2016.1405info:eu-repo/semantics/altIdentifier/doi/10.1089/zeb.2016.1405info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T09:44:34Zoai:ri.conicet.gov.ar:11336/46291instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 09:44:35.015CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Highest diploid number among Gymnotiformes: first cytogenetic insights into Rhabdolichops (Sternopygidae)
title Highest diploid number among Gymnotiformes: first cytogenetic insights into Rhabdolichops (Sternopygidae)
spellingShingle Highest diploid number among Gymnotiformes: first cytogenetic insights into Rhabdolichops (Sternopygidae)
Suarez, Pablo
Electric Fish
Chromosomes
Biodiversity
Amazon Rainforest
Kariyotypic Diversity
title_short Highest diploid number among Gymnotiformes: first cytogenetic insights into Rhabdolichops (Sternopygidae)
title_full Highest diploid number among Gymnotiformes: first cytogenetic insights into Rhabdolichops (Sternopygidae)
title_fullStr Highest diploid number among Gymnotiformes: first cytogenetic insights into Rhabdolichops (Sternopygidae)
title_full_unstemmed Highest diploid number among Gymnotiformes: first cytogenetic insights into Rhabdolichops (Sternopygidae)
title_sort Highest diploid number among Gymnotiformes: first cytogenetic insights into Rhabdolichops (Sternopygidae)
dc.creator.none.fl_str_mv Suarez, Pablo
Pinto Barroso, Inaê Cristina Guerreiro
Silva, Danilo dos Santos
Milhomem, Susana S. R.
Cabral de Mello, Diogo Cavalcanti
Martins, Cesar
Pieczarka, Julio Cesar
Nagamachi, Cleusa Yoshiko
author Suarez, Pablo
author_facet Suarez, Pablo
Pinto Barroso, Inaê Cristina Guerreiro
Silva, Danilo dos Santos
Milhomem, Susana S. R.
Cabral de Mello, Diogo Cavalcanti
Martins, Cesar
Pieczarka, Julio Cesar
Nagamachi, Cleusa Yoshiko
author_role author
author2 Pinto Barroso, Inaê Cristina Guerreiro
Silva, Danilo dos Santos
Milhomem, Susana S. R.
Cabral de Mello, Diogo Cavalcanti
Martins, Cesar
Pieczarka, Julio Cesar
Nagamachi, Cleusa Yoshiko
author2_role author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Electric Fish
Chromosomes
Biodiversity
Amazon Rainforest
Kariyotypic Diversity
topic Electric Fish
Chromosomes
Biodiversity
Amazon Rainforest
Kariyotypic Diversity
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv We report the first comparative cytogenetic analysis of two species from electrogenic fish of genus Rhabdolichops (Sternopygidae, Gymnotiformes): Rhabdolichops troscheli and Rhabdolichops cf eastwardi. R. troscheli has 2n = 54 (fundamental number [FN] = 66), whereas R. cf. eastwardi has 2n = 74 (FN = 78). C-banding revealed centromeric constitutive heterochromatin in both species. Ag-NORs mapped on pair 6 in R. troscheli and pair 30 in R. cf eastwardi. Fluorescense in situ hybridization with 18S rDNA probes confirmed the Ag-NOR staining results and revealed additional (presumably silent) ribosomal genes on pairs 12, 13, 21, 23, 26, and 27 in R. cf eastwardi. 5S rDNA was found on the centromeres of pair 7 in both species. Telomeric probes showed only distal locations. Dispersed signal patterns were obtained using probes for retrotransposons Rex1 and Rex3. Histone H1 and H3 genes were found together on pair 6 in R. cf eastwardi. The high diploid number found in Rhabdolichops suggests that chromosome fission may have contributed to its chromosomal evolution, phylogenetic relationship of the Sternopygidae suggests that this increase in diploid number could be a synapomorphic characteristic of genus Rhabdolichops. Although both species are phylogenetically close related, their karyotype structure has undergone divergent evolutionary directions. All in all, our results strongly suggest that R. cf eastwardi experencied recent intense genome reorganization.
Fil: Suarez, Pablo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste. Instituto de Biología Subtropical. Instituto de Biología Subtropical - Nodo Puerto Iguazú | Universidad Nacional de Misiones. Instituto de Biología Subtropical. Instituto de Biología Subtropical - Nodo Puerto Iguazú; Argentina. Universidade Federal do Pará; Brasil
Fil: Pinto Barroso, Inaê Cristina Guerreiro. Universidade Federal do Pará; Brasil
Fil: Silva, Danilo dos Santos. Universidade do Estado de Pará; Brasil
Fil: Milhomem, Susana S. R.. Instituto Federal de Educação, Ciência e Tecnologia de Góias; Brasil
Fil: Cabral de Mello, Diogo Cavalcanti. Universidade Estadual Paulista Julio de Mesquita Filho; Brasil
Fil: Martins, Cesar. Universidade Estadual Paulista Julio de Mesquita Filho; Brasil
Fil: Pieczarka, Julio Cesar. Universidade Federal do Pará; Brasil
Fil: Nagamachi, Cleusa Yoshiko. Universidade Federal do Pará; Brasil
description We report the first comparative cytogenetic analysis of two species from electrogenic fish of genus Rhabdolichops (Sternopygidae, Gymnotiformes): Rhabdolichops troscheli and Rhabdolichops cf eastwardi. R. troscheli has 2n = 54 (fundamental number [FN] = 66), whereas R. cf. eastwardi has 2n = 74 (FN = 78). C-banding revealed centromeric constitutive heterochromatin in both species. Ag-NORs mapped on pair 6 in R. troscheli and pair 30 in R. cf eastwardi. Fluorescense in situ hybridization with 18S rDNA probes confirmed the Ag-NOR staining results and revealed additional (presumably silent) ribosomal genes on pairs 12, 13, 21, 23, 26, and 27 in R. cf eastwardi. 5S rDNA was found on the centromeres of pair 7 in both species. Telomeric probes showed only distal locations. Dispersed signal patterns were obtained using probes for retrotransposons Rex1 and Rex3. Histone H1 and H3 genes were found together on pair 6 in R. cf eastwardi. The high diploid number found in Rhabdolichops suggests that chromosome fission may have contributed to its chromosomal evolution, phylogenetic relationship of the Sternopygidae suggests that this increase in diploid number could be a synapomorphic characteristic of genus Rhabdolichops. Although both species are phylogenetically close related, their karyotype structure has undergone divergent evolutionary directions. All in all, our results strongly suggest that R. cf eastwardi experencied recent intense genome reorganization.
publishDate 2017
dc.date.none.fl_str_mv 2017-06
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/46291
Suarez, Pablo; Pinto Barroso, Inaê Cristina Guerreiro; Silva, Danilo dos Santos; Milhomem, Susana S. R.; Cabral de Mello, Diogo Cavalcanti; et al.; Highest diploid number among Gymnotiformes: first cytogenetic insights into Rhabdolichops (Sternopygidae); Mary Ann Liebert; Zebrafish; 14; 3; 6-2017; 272-279
1545-8547
1557-8542
CONICET Digital
CONICET
url http://hdl.handle.net/11336/46291
identifier_str_mv Suarez, Pablo; Pinto Barroso, Inaê Cristina Guerreiro; Silva, Danilo dos Santos; Milhomem, Susana S. R.; Cabral de Mello, Diogo Cavalcanti; et al.; Highest diploid number among Gymnotiformes: first cytogenetic insights into Rhabdolichops (Sternopygidae); Mary Ann Liebert; Zebrafish; 14; 3; 6-2017; 272-279
1545-8547
1557-8542
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/http://online.liebertpub.com/doi/full/10.1089/zeb.2016.1405
info:eu-repo/semantics/altIdentifier/doi/10.1089/zeb.2016.1405
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Mary Ann Liebert
publisher.none.fl_str_mv Mary Ann Liebert
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1842268675793485824
score 13.13397

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