Comparative genomic mapping reveals mechanisms of chromosome diversification in Rhipidomys species (Rodentia, Thomasomyini) and syntenic relationship between species of Sigmodontin...

Autores
Dos Santos Paixão, Vergiana; Suarez, Pablo; Oliveira da Silva, Willam; Geise, Lena; Ferguson Smith, Malcolm Andrew; Mary O’Brien, Patricia Caroline; Mendes Oliveira, Ana Cristina; Vieira Rossi, Rogério; Pieczarka, Julio Cesar; Nagamachi, Cleusa Yoshiko
Año de publicación
2021
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Rhipidomys (Sigmodontinae, Thomasomyini) has 25 recognized species, with a wide distribution ranging from eastern Panama to northern Argentina. Cytogenetic data has been described for 13 species with 12 of them having 2n = 44 with a high level of autosomal fundamental number (FN) variation, ranging from 46 to 80, assigned to pericentric inversions. The species are grouped in groups with low FN (46-52) and high FN (72-80). In this work the karyotypes of Rhipidomys emiliae (2n = 44, FN = 50) and Rhipidomys mastacalis (2n = 44, FN = 74), were studied by classical cytogenetics and by fluorescence in situ hybridization using telomeric and whole chromosome probes (chromosome painting) of Hylaeamys megacephalus (HME). Chromosome painting revealed homology between 36 segments of REM and 37 of RMA. We tested the hypothesis that pericentric inversions are the predominant chromosomal rearrangements responsible for karyotypic divergence between these species, as proposed in literature. Our results show that the genomic diversification between the karyotypes of the two species resulted from translocations, centromeric repositioning and pericentric inversions. The chromosomal evolution in Rhipidomys was associatedwith karyotypical orthoselection. The HME probes revealed that seven syntenic probably ancestral blocks for Sigmodontinae are present in Rhipidomys. An additional syntenic block described here is suggested as part of the subfamily ancestral karyotype. We also define five synapomorphies that can be used as chromosomal signatures for Rhipidomys.
Fil: Dos Santos Paixão, Vergiana. Universidade Federal do Pará; Brasil
Fil: Suarez, Pablo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste. Instituto de Biología Subtropical. Instituto de Biología Subtropical - Nodo Puerto Iguazú | Universidad Nacional de Misiones. Instituto de Biología Subtropical. Instituto de Biología Subtropical - Nodo Puerto Iguazú; Argentina
Fil: Oliveira da Silva, Willam. Universidade Federal do Pará; Brasil
Fil: Geise, Lena. Universidade do Estado de Rio do Janeiro; Brasil
Fil: Ferguson Smith, Malcolm Andrew. Cambridge Resource Centre For Comparative Genomics; Reino Unido
Fil: Mary O’Brien, Patricia Caroline. University of Cambridge; Reino Unido
Fil: Mendes Oliveira, Ana Cristina. Universidade Federal do Pará; Brasil
Fil: Vieira Rossi, Rogério. Universidade Federal do Mato Grosso do Sul; Brasil
Fil: Pieczarka, Julio Cesar. Universidade Federal do Pará; Brasil
Fil: Nagamachi, Cleusa Yoshiko. Universidade Federal do Pará; Brasil
Materia
SMALL TREE RATS
AMAZON RAINFOREST
ATLANTIC FOREST
CHROMOSOME PAINTING
CHROMOSOMAL SYNAPOMORPHIES
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/155540
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/155540
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Comparative genomic mapping reveals mechanisms of chromosome diversification in Rhipidomys species (Rodentia, Thomasomyini) and syntenic relationship between species of SigmodontinaeDos Santos Paixão, VergianaSuarez, PabloOliveira da Silva, WillamGeise, LenaFerguson Smith, Malcolm AndrewMary O’Brien, Patricia CarolineMendes Oliveira, Ana CristinaVieira Rossi, RogérioPieczarka, Julio CesarNagamachi, Cleusa YoshikoSMALL TREE RATSAMAZON RAINFORESTATLANTIC FORESTCHROMOSOME PAINTINGCHROMOSOMAL SYNAPOMORPHIEShttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1https://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Rhipidomys (Sigmodontinae, Thomasomyini) has 25 recognized species, with a wide distribution ranging from eastern Panama to northern Argentina. Cytogenetic data has been described for 13 species with 12 of them having 2n = 44 with a high level of autosomal fundamental number (FN) variation, ranging from 46 to 80, assigned to pericentric inversions. The species are grouped in groups with low FN (46-52) and high FN (72-80). In this work the karyotypes of Rhipidomys emiliae (2n = 44, FN = 50) and Rhipidomys mastacalis (2n = 44, FN = 74), were studied by classical cytogenetics and by fluorescence in situ hybridization using telomeric and whole chromosome probes (chromosome painting) of Hylaeamys megacephalus (HME). Chromosome painting revealed homology between 36 segments of REM and 37 of RMA. We tested the hypothesis that pericentric inversions are the predominant chromosomal rearrangements responsible for karyotypic divergence between these species, as proposed in literature. Our results show that the genomic diversification between the karyotypes of the two species resulted from translocations, centromeric repositioning and pericentric inversions. The chromosomal evolution in Rhipidomys was associatedwith karyotypical orthoselection. The HME probes revealed that seven syntenic probably ancestral blocks for Sigmodontinae are present in Rhipidomys. An additional syntenic block described here is suggested as part of the subfamily ancestral karyotype. We also define five synapomorphies that can be used as chromosomal signatures for Rhipidomys.Fil: Dos Santos Paixão, Vergiana. Universidade Federal do Pará; BrasilFil: Suarez, Pablo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste. Instituto de Biología Subtropical. Instituto de Biología Subtropical - Nodo Puerto Iguazú | Universidad Nacional de Misiones. Instituto de Biología Subtropical. Instituto de Biología Subtropical - Nodo Puerto Iguazú; ArgentinaFil: Oliveira da Silva, Willam. Universidade Federal do Pará; BrasilFil: Geise, Lena. Universidade do Estado de Rio do Janeiro; BrasilFil: Ferguson Smith, Malcolm Andrew. Cambridge Resource Centre For Comparative Genomics; Reino UnidoFil: Mary O’Brien, Patricia Caroline. University of Cambridge; Reino UnidoFil: Mendes Oliveira, Ana Cristina. Universidade Federal do Pará; BrasilFil: Vieira Rossi, Rogério. Universidade Federal do Mato Grosso do Sul; BrasilFil: Pieczarka, Julio Cesar. Universidade Federal do Pará; BrasilFil: Nagamachi, Cleusa Yoshiko. Universidade Federal do Pará; BrasilPublic Library of Science2021-10info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/155540Dos Santos Paixão, Vergiana; Suarez, Pablo; Oliveira da Silva, Willam; Geise, Lena ; Ferguson Smith, Malcolm Andrew; et al.; Comparative genomic mapping reveals mechanisms of chromosome diversification in Rhipidomys species (Rodentia, Thomasomyini) and syntenic relationship between species of Sigmodontinae; Public Library of Science; Plos One; 10-2021; 1-211932-6203CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0258474info:eu-repo/semantics/altIdentifier/doi/10.1371/journal.pone.0258474info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T10:01:21Zoai:ri.conicet.gov.ar:11336/155540instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 10:01:21.506CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Comparative genomic mapping reveals mechanisms of chromosome diversification in Rhipidomys species (Rodentia, Thomasomyini) and syntenic relationship between species of Sigmodontinae
title Comparative genomic mapping reveals mechanisms of chromosome diversification in Rhipidomys species (Rodentia, Thomasomyini) and syntenic relationship between species of Sigmodontinae
spellingShingle Comparative genomic mapping reveals mechanisms of chromosome diversification in Rhipidomys species (Rodentia, Thomasomyini) and syntenic relationship between species of Sigmodontinae
Dos Santos Paixão, Vergiana
SMALL TREE RATS
AMAZON RAINFOREST
ATLANTIC FOREST
CHROMOSOME PAINTING
CHROMOSOMAL SYNAPOMORPHIES
title_short Comparative genomic mapping reveals mechanisms of chromosome diversification in Rhipidomys species (Rodentia, Thomasomyini) and syntenic relationship between species of Sigmodontinae
title_full Comparative genomic mapping reveals mechanisms of chromosome diversification in Rhipidomys species (Rodentia, Thomasomyini) and syntenic relationship between species of Sigmodontinae
title_fullStr Comparative genomic mapping reveals mechanisms of chromosome diversification in Rhipidomys species (Rodentia, Thomasomyini) and syntenic relationship between species of Sigmodontinae
title_full_unstemmed Comparative genomic mapping reveals mechanisms of chromosome diversification in Rhipidomys species (Rodentia, Thomasomyini) and syntenic relationship between species of Sigmodontinae
title_sort Comparative genomic mapping reveals mechanisms of chromosome diversification in Rhipidomys species (Rodentia, Thomasomyini) and syntenic relationship between species of Sigmodontinae
dc.creator.none.fl_str_mv Dos Santos Paixão, Vergiana
Suarez, Pablo
Oliveira da Silva, Willam
Geise, Lena
Ferguson Smith, Malcolm Andrew
Mary O’Brien, Patricia Caroline
Mendes Oliveira, Ana Cristina
Vieira Rossi, Rogério
Pieczarka, Julio Cesar
Nagamachi, Cleusa Yoshiko
author Dos Santos Paixão, Vergiana
author_facet Dos Santos Paixão, Vergiana
Suarez, Pablo
Oliveira da Silva, Willam
Geise, Lena
Ferguson Smith, Malcolm Andrew
Mary O’Brien, Patricia Caroline
Mendes Oliveira, Ana Cristina
Vieira Rossi, Rogério
Pieczarka, Julio Cesar
Nagamachi, Cleusa Yoshiko
author_role author
author2 Suarez, Pablo
Oliveira da Silva, Willam
Geise, Lena
Ferguson Smith, Malcolm Andrew
Mary O’Brien, Patricia Caroline
Mendes Oliveira, Ana Cristina
Vieira Rossi, Rogério
Pieczarka, Julio Cesar
Nagamachi, Cleusa Yoshiko
author2_role author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv SMALL TREE RATS
AMAZON RAINFOREST
ATLANTIC FOREST
CHROMOSOME PAINTING
CHROMOSOMAL SYNAPOMORPHIES
topic SMALL TREE RATS
AMAZON RAINFOREST
ATLANTIC FOREST
CHROMOSOME PAINTING
CHROMOSOMAL SYNAPOMORPHIES
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Rhipidomys (Sigmodontinae, Thomasomyini) has 25 recognized species, with a wide distribution ranging from eastern Panama to northern Argentina. Cytogenetic data has been described for 13 species with 12 of them having 2n = 44 with a high level of autosomal fundamental number (FN) variation, ranging from 46 to 80, assigned to pericentric inversions. The species are grouped in groups with low FN (46-52) and high FN (72-80). In this work the karyotypes of Rhipidomys emiliae (2n = 44, FN = 50) and Rhipidomys mastacalis (2n = 44, FN = 74), were studied by classical cytogenetics and by fluorescence in situ hybridization using telomeric and whole chromosome probes (chromosome painting) of Hylaeamys megacephalus (HME). Chromosome painting revealed homology between 36 segments of REM and 37 of RMA. We tested the hypothesis that pericentric inversions are the predominant chromosomal rearrangements responsible for karyotypic divergence between these species, as proposed in literature. Our results show that the genomic diversification between the karyotypes of the two species resulted from translocations, centromeric repositioning and pericentric inversions. The chromosomal evolution in Rhipidomys was associatedwith karyotypical orthoselection. The HME probes revealed that seven syntenic probably ancestral blocks for Sigmodontinae are present in Rhipidomys. An additional syntenic block described here is suggested as part of the subfamily ancestral karyotype. We also define five synapomorphies that can be used as chromosomal signatures for Rhipidomys.
Fil: Dos Santos Paixão, Vergiana. Universidade Federal do Pará; Brasil
Fil: Suarez, Pablo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste. Instituto de Biología Subtropical. Instituto de Biología Subtropical - Nodo Puerto Iguazú | Universidad Nacional de Misiones. Instituto de Biología Subtropical. Instituto de Biología Subtropical - Nodo Puerto Iguazú; Argentina
Fil: Oliveira da Silva, Willam. Universidade Federal do Pará; Brasil
Fil: Geise, Lena. Universidade do Estado de Rio do Janeiro; Brasil
Fil: Ferguson Smith, Malcolm Andrew. Cambridge Resource Centre For Comparative Genomics; Reino Unido
Fil: Mary O’Brien, Patricia Caroline. University of Cambridge; Reino Unido
Fil: Mendes Oliveira, Ana Cristina. Universidade Federal do Pará; Brasil
Fil: Vieira Rossi, Rogério. Universidade Federal do Mato Grosso do Sul; Brasil
Fil: Pieczarka, Julio Cesar. Universidade Federal do Pará; Brasil
Fil: Nagamachi, Cleusa Yoshiko. Universidade Federal do Pará; Brasil
description Rhipidomys (Sigmodontinae, Thomasomyini) has 25 recognized species, with a wide distribution ranging from eastern Panama to northern Argentina. Cytogenetic data has been described for 13 species with 12 of them having 2n = 44 with a high level of autosomal fundamental number (FN) variation, ranging from 46 to 80, assigned to pericentric inversions. The species are grouped in groups with low FN (46-52) and high FN (72-80). In this work the karyotypes of Rhipidomys emiliae (2n = 44, FN = 50) and Rhipidomys mastacalis (2n = 44, FN = 74), were studied by classical cytogenetics and by fluorescence in situ hybridization using telomeric and whole chromosome probes (chromosome painting) of Hylaeamys megacephalus (HME). Chromosome painting revealed homology between 36 segments of REM and 37 of RMA. We tested the hypothesis that pericentric inversions are the predominant chromosomal rearrangements responsible for karyotypic divergence between these species, as proposed in literature. Our results show that the genomic diversification between the karyotypes of the two species resulted from translocations, centromeric repositioning and pericentric inversions. The chromosomal evolution in Rhipidomys was associatedwith karyotypical orthoselection. The HME probes revealed that seven syntenic probably ancestral blocks for Sigmodontinae are present in Rhipidomys. An additional syntenic block described here is suggested as part of the subfamily ancestral karyotype. We also define five synapomorphies that can be used as chromosomal signatures for Rhipidomys.
publishDate 2021
dc.date.none.fl_str_mv 2021-10
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/155540
Dos Santos Paixão, Vergiana; Suarez, Pablo; Oliveira da Silva, Willam; Geise, Lena ; Ferguson Smith, Malcolm Andrew; et al.; Comparative genomic mapping reveals mechanisms of chromosome diversification in Rhipidomys species (Rodentia, Thomasomyini) and syntenic relationship between species of Sigmodontinae; Public Library of Science; Plos One; 10-2021; 1-21
1932-6203
CONICET Digital
CONICET
url http://hdl.handle.net/11336/155540
identifier_str_mv Dos Santos Paixão, Vergiana; Suarez, Pablo; Oliveira da Silva, Willam; Geise, Lena ; Ferguson Smith, Malcolm Andrew; et al.; Comparative genomic mapping reveals mechanisms of chromosome diversification in Rhipidomys species (Rodentia, Thomasomyini) and syntenic relationship between species of Sigmodontinae; Public Library of Science; Plos One; 10-2021; 1-21
1932-6203
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0258474
info:eu-repo/semantics/altIdentifier/doi/10.1371/journal.pone.0258474
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Public Library of Science
publisher.none.fl_str_mv Public Library of Science
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.13397

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