Cytogenetic studies in herbertia sw. (iridaceae)

Autores
Moreno, Natalia Cecilia; Las Peñas, Maria Laura; Bernardello, Gabriel Luis Mario; Roitman, Germán
Año de publicación
2009
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Conventional Feulgen staining, CMA/DAPI banding and FISH with 18-5.8-26S rDNA probe were used to determine chromosome numbers, karyotypes and physical position of GC or AT rich heterochromatin and rDNA sites in three South American species of Herbertia: H. lahue subsp. amoena, H. quareimana, and H. darwinii. The basic number of the genus is x=7, but the analyzed taxa have different ploidy levels. The chromosome number was 2n=14 for H. darwinii and 2n=28 for H. quareimana. The two populations studied of H. lahue subsp. amoena did result in: 2n=42 (Gualeguaychu) and 56 (Palma de Castillo). Chromosome size varied between 1.60 and 5 µm. The karyotypes did give with a total of 8 metacentric (m) + 6 submetacentric (sm) chromosomes for H. darwinii and 20 m + 8 sm chromosomes for H. quareimana. The two populations of H. lahue subsp. amoena showed with 26 m + 16 sm chromosomes and 36 m + 20 sm chromosomes. H. darwinii had a bimodal karyotype. H. darwinii showed one satellited pair whereas the remaining species two (always in short m chromosome arms). Moreover, the 2n=56 population of H. lahue subsp. amoena one satellite on the long arms. No CMA- /DAPI+ bands were detected in any species, but CMA+/DAPI– bands associated with nucleolar organizing regions (NOR) were found in all cases. The locations of the 18-5.8-26S rDNA sites in all species coincided with CMA+/DAPI- bands. Our data suggest that karyotypic diversification in Herbertia included changes in chromosome number, symmetry, and number and position of NORs regions.
Fil: Moreno, Natalia Cecilia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto Multidisciplinario de Biología Vegetal. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas Físicas y Naturales. Instituto Multidisciplinario de Biología Vegetal; Argentina
Fil: Las Peñas, Maria Laura. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto Multidisciplinario de Biología Vegetal. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas Físicas y Naturales. Instituto Multidisciplinario de Biología Vegetal; Argentina
Fil: Bernardello, Gabriel Luis Mario. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto Multidisciplinario de Biología Vegetal. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas Físicas y Naturales. Instituto Multidisciplinario de Biología Vegetal; Argentina
Fil: Roitman, Germán. Universidad de Buenos Aires. Facultad de Agronomia; Argentina
Materia
CMADAPI BANDING
FISH
HERBERTIA
KARYOTYPE
POLYPLOIDY
SOMATIC CHROMOSOMES
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/20607
Acceder
id CONICETDig_8ac8cebcf86c5b86119deda639eb124d
oai_identifier_str oai:ri.conicet.gov.ar:11336/20607
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Cytogenetic studies in herbertia sw. (iridaceae)Moreno, Natalia CeciliaLas Peñas, Maria LauraBernardello, Gabriel Luis MarioRoitman, GermánCMADAPI BANDINGFISHHERBERTIAKARYOTYPEPOLYPLOIDYSOMATIC CHROMOSOMEShttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Conventional Feulgen staining, CMA/DAPI banding and FISH with 18-5.8-26S rDNA probe were used to determine chromosome numbers, karyotypes and physical position of GC or AT rich heterochromatin and rDNA sites in three South American species of Herbertia: H. lahue subsp. amoena, H. quareimana, and H. darwinii. The basic number of the genus is x=7, but the analyzed taxa have different ploidy levels. The chromosome number was 2n=14 for H. darwinii and 2n=28 for H. quareimana. The two populations studied of H. lahue subsp. amoena did result in: 2n=42 (Gualeguaychu) and 56 (Palma de Castillo). Chromosome size varied between 1.60 and 5 µm. The karyotypes did give with a total of 8 metacentric (m) + 6 submetacentric (sm) chromosomes for H. darwinii and 20 m + 8 sm chromosomes for H. quareimana. The two populations of H. lahue subsp. amoena showed with 26 m + 16 sm chromosomes and 36 m + 20 sm chromosomes. H. darwinii had a bimodal karyotype. H. darwinii showed one satellited pair whereas the remaining species two (always in short m chromosome arms). Moreover, the 2n=56 population of H. lahue subsp. amoena one satellite on the long arms. No CMA- /DAPI+ bands were detected in any species, but CMA+/DAPI– bands associated with nucleolar organizing regions (NOR) were found in all cases. The locations of the 18-5.8-26S rDNA sites in all species coincided with CMA+/DAPI- bands. Our data suggest that karyotypic diversification in Herbertia included changes in chromosome number, symmetry, and number and position of NORs regions.Fil: Moreno, Natalia Cecilia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto Multidisciplinario de Biología Vegetal. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas Físicas y Naturales. Instituto Multidisciplinario de Biología Vegetal; ArgentinaFil: Las Peñas, Maria Laura. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto Multidisciplinario de Biología Vegetal. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas Físicas y Naturales. Instituto Multidisciplinario de Biología Vegetal; ArgentinaFil: Bernardello, Gabriel Luis Mario. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto Multidisciplinario de Biología Vegetal. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas Físicas y Naturales. Instituto Multidisciplinario de Biología Vegetal; ArgentinaFil: Roitman, Germán. Universidad de Buenos Aires. Facultad de Agronomia; ArgentinaUniversity Florence Botany Institute2009-12info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/20607Moreno, Natalia Cecilia; Las Peñas, Maria Laura; Bernardello, Gabriel Luis Mario; Roitman, Germán; Cytogenetic studies in herbertia sw. (iridaceae); University Florence Botany Institute; Caryologia; 62; 1; 12-2009; 37-420008-71142165-5391CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/http://www.tandfonline.com/doi/pdf/10.1080/00087114.2004.10589664info:eu-repo/semantics/altIdentifier/doi/10.1080/00087114.2004.10589664info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T10:03:37Zoai:ri.conicet.gov.ar:11336/20607instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 10:03:37.742CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Cytogenetic studies in herbertia sw. (iridaceae)
title Cytogenetic studies in herbertia sw. (iridaceae)
spellingShingle Cytogenetic studies in herbertia sw. (iridaceae)
Moreno, Natalia Cecilia
CMADAPI BANDING
FISH
HERBERTIA
KARYOTYPE
POLYPLOIDY
SOMATIC CHROMOSOMES
title_short Cytogenetic studies in herbertia sw. (iridaceae)
title_full Cytogenetic studies in herbertia sw. (iridaceae)
title_fullStr Cytogenetic studies in herbertia sw. (iridaceae)
title_full_unstemmed Cytogenetic studies in herbertia sw. (iridaceae)
title_sort Cytogenetic studies in herbertia sw. (iridaceae)
dc.creator.none.fl_str_mv Moreno, Natalia Cecilia
Las Peñas, Maria Laura
Bernardello, Gabriel Luis Mario
Roitman, Germán
author Moreno, Natalia Cecilia
author_facet Moreno, Natalia Cecilia
Las Peñas, Maria Laura
Bernardello, Gabriel Luis Mario
Roitman, Germán
author_role author
author2 Las Peñas, Maria Laura
Bernardello, Gabriel Luis Mario
Roitman, Germán
author2_role author
author
author
dc.subject.none.fl_str_mv CMADAPI BANDING
FISH
HERBERTIA
KARYOTYPE
POLYPLOIDY
SOMATIC CHROMOSOMES
topic CMADAPI BANDING
FISH
HERBERTIA
KARYOTYPE
POLYPLOIDY
SOMATIC CHROMOSOMES
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Conventional Feulgen staining, CMA/DAPI banding and FISH with 18-5.8-26S rDNA probe were used to determine chromosome numbers, karyotypes and physical position of GC or AT rich heterochromatin and rDNA sites in three South American species of Herbertia: H. lahue subsp. amoena, H. quareimana, and H. darwinii. The basic number of the genus is x=7, but the analyzed taxa have different ploidy levels. The chromosome number was 2n=14 for H. darwinii and 2n=28 for H. quareimana. The two populations studied of H. lahue subsp. amoena did result in: 2n=42 (Gualeguaychu) and 56 (Palma de Castillo). Chromosome size varied between 1.60 and 5 µm. The karyotypes did give with a total of 8 metacentric (m) + 6 submetacentric (sm) chromosomes for H. darwinii and 20 m + 8 sm chromosomes for H. quareimana. The two populations of H. lahue subsp. amoena showed with 26 m + 16 sm chromosomes and 36 m + 20 sm chromosomes. H. darwinii had a bimodal karyotype. H. darwinii showed one satellited pair whereas the remaining species two (always in short m chromosome arms). Moreover, the 2n=56 population of H. lahue subsp. amoena one satellite on the long arms. No CMA- /DAPI+ bands were detected in any species, but CMA+/DAPI– bands associated with nucleolar organizing regions (NOR) were found in all cases. The locations of the 18-5.8-26S rDNA sites in all species coincided with CMA+/DAPI- bands. Our data suggest that karyotypic diversification in Herbertia included changes in chromosome number, symmetry, and number and position of NORs regions.
Fil: Moreno, Natalia Cecilia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto Multidisciplinario de Biología Vegetal. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas Físicas y Naturales. Instituto Multidisciplinario de Biología Vegetal; Argentina
Fil: Las Peñas, Maria Laura. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto Multidisciplinario de Biología Vegetal. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas Físicas y Naturales. Instituto Multidisciplinario de Biología Vegetal; Argentina
Fil: Bernardello, Gabriel Luis Mario. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto Multidisciplinario de Biología Vegetal. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas Físicas y Naturales. Instituto Multidisciplinario de Biología Vegetal; Argentina
Fil: Roitman, Germán. Universidad de Buenos Aires. Facultad de Agronomia; Argentina
description Conventional Feulgen staining, CMA/DAPI banding and FISH with 18-5.8-26S rDNA probe were used to determine chromosome numbers, karyotypes and physical position of GC or AT rich heterochromatin and rDNA sites in three South American species of Herbertia: H. lahue subsp. amoena, H. quareimana, and H. darwinii. The basic number of the genus is x=7, but the analyzed taxa have different ploidy levels. The chromosome number was 2n=14 for H. darwinii and 2n=28 for H. quareimana. The two populations studied of H. lahue subsp. amoena did result in: 2n=42 (Gualeguaychu) and 56 (Palma de Castillo). Chromosome size varied between 1.60 and 5 µm. The karyotypes did give with a total of 8 metacentric (m) + 6 submetacentric (sm) chromosomes for H. darwinii and 20 m + 8 sm chromosomes for H. quareimana. The two populations of H. lahue subsp. amoena showed with 26 m + 16 sm chromosomes and 36 m + 20 sm chromosomes. H. darwinii had a bimodal karyotype. H. darwinii showed one satellited pair whereas the remaining species two (always in short m chromosome arms). Moreover, the 2n=56 population of H. lahue subsp. amoena one satellite on the long arms. No CMA- /DAPI+ bands were detected in any species, but CMA+/DAPI– bands associated with nucleolar organizing regions (NOR) were found in all cases. The locations of the 18-5.8-26S rDNA sites in all species coincided with CMA+/DAPI- bands. Our data suggest that karyotypic diversification in Herbertia included changes in chromosome number, symmetry, and number and position of NORs regions.
publishDate 2009
dc.date.none.fl_str_mv 2009-12
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/20607
Moreno, Natalia Cecilia; Las Peñas, Maria Laura; Bernardello, Gabriel Luis Mario; Roitman, Germán; Cytogenetic studies in herbertia sw. (iridaceae); University Florence Botany Institute; Caryologia; 62; 1; 12-2009; 37-42
0008-7114
2165-5391
CONICET Digital
CONICET
url http://hdl.handle.net/11336/20607
identifier_str_mv Moreno, Natalia Cecilia; Las Peñas, Maria Laura; Bernardello, Gabriel Luis Mario; Roitman, Germán; Cytogenetic studies in herbertia sw. (iridaceae); University Florence Botany Institute; Caryologia; 62; 1; 12-2009; 37-42
0008-7114
2165-5391
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/http://www.tandfonline.com/doi/pdf/10.1080/00087114.2004.10589664
info:eu-repo/semantics/altIdentifier/doi/10.1080/00087114.2004.10589664
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv University Florence Botany Institute
publisher.none.fl_str_mv University Florence Botany Institute
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.13397

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