Vaccination of guinea pigs using mce operon mutants of Mycobacterium tuberculosis

Autores
Obregón Henao, Andrés; Shanley, Crystal; Bianco, María Verónica; Cataldi, Ángel Adrián; Basaraba, Randall J.; Orme, Ian M.; Bigi, Fabiana
Año de publicación
2011
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The limited efficacy of the BCG vaccine for tuberculosis, coupled with emerging information suggesting that it is poorly protective against newly emerging strains of Mycobacterium tuberculosis such as the W-Beijing isolates, makes it paramount to search for more potent alternatives. One such class of candidates is attenuated mutants derived from M. tuberculosis itself. We demonstrate here, in an initial short term assay, that mutants derived from disruption of the mce genes of the bacillus were highly protective in guinea pigs exposed by low dose aerosol infection with the virulent W-Beijing isolate SA161. This protection was demonstrated by a significant reduction in the numbers of bacilli harvested from the lungs, and dramatic improvements in lung histopathology.
Fil: Obregón Henao, Andrés. State University of Colorado - Fort Collins; Estados Unidos
Fil: Shanley, Crystal. State University of Colorado - Fort Collins; Estados Unidos
Fil: Bianco, María Verónica. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Biotecnología; Argentina
Fil: Cataldi, Ángel Adrián. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Basaraba, Randall J.. State University of Colorado - Fort Collins; Estados Unidos
Fil: Orme, Ian M.. State University of Colorado - Fort Collins; Estados Unidos
Fil: Bigi, Fabiana. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Biotecnología; Argentina
Materia
ATTENUATED MUTANTS
GUINEA PIG
TUBERCULOSIS
VACCINATION
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/190048
Acceder
id CONICETDig_e983c56f88031f23efec7b23aa819588
oai_identifier_str oai:ri.conicet.gov.ar:11336/190048
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Vaccination of guinea pigs using mce operon mutants of Mycobacterium tuberculosisObregón Henao, AndrésShanley, CrystalBianco, María VerónicaCataldi, Ángel AdriánBasaraba, Randall J.Orme, Ian M.Bigi, FabianaATTENUATED MUTANTSGUINEA PIGTUBERCULOSISVACCINATIONhttps://purl.org/becyt/ford/1.7https://purl.org/becyt/ford/1The limited efficacy of the BCG vaccine for tuberculosis, coupled with emerging information suggesting that it is poorly protective against newly emerging strains of Mycobacterium tuberculosis such as the W-Beijing isolates, makes it paramount to search for more potent alternatives. One such class of candidates is attenuated mutants derived from M. tuberculosis itself. We demonstrate here, in an initial short term assay, that mutants derived from disruption of the mce genes of the bacillus were highly protective in guinea pigs exposed by low dose aerosol infection with the virulent W-Beijing isolate SA161. This protection was demonstrated by a significant reduction in the numbers of bacilli harvested from the lungs, and dramatic improvements in lung histopathology.Fil: Obregón Henao, Andrés. State University of Colorado - Fort Collins; Estados UnidosFil: Shanley, Crystal. State University of Colorado - Fort Collins; Estados UnidosFil: Bianco, María Verónica. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Biotecnología; ArgentinaFil: Cataldi, Ángel Adrián. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Basaraba, Randall J.. State University of Colorado - Fort Collins; Estados UnidosFil: Orme, Ian M.. State University of Colorado - Fort Collins; Estados UnidosFil: Bigi, Fabiana. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Biotecnología; ArgentinaElsevier2011-08info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/190048Obregón Henao, Andrés; Shanley, Crystal; Bianco, María Verónica; Cataldi, Ángel Adrián; Basaraba, Randall J.; et al.; Vaccination of guinea pigs using mce operon mutants of Mycobacterium tuberculosis; Elsevier; Vaccine; 29; 26; 8-2011; 4302-43070264-410XCONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S0264410X11005627info:eu-repo/semantics/altIdentifier/doi/10.1016/j.vaccine.2011.04.027info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-15T15:08:52Zoai:ri.conicet.gov.ar:11336/190048instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-15 15:08:53.289CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Vaccination of guinea pigs using mce operon mutants of Mycobacterium tuberculosis
title Vaccination of guinea pigs using mce operon mutants of Mycobacterium tuberculosis
spellingShingle Vaccination of guinea pigs using mce operon mutants of Mycobacterium tuberculosis
Obregón Henao, Andrés
ATTENUATED MUTANTS
GUINEA PIG
TUBERCULOSIS
VACCINATION
title_short Vaccination of guinea pigs using mce operon mutants of Mycobacterium tuberculosis
title_full Vaccination of guinea pigs using mce operon mutants of Mycobacterium tuberculosis
title_fullStr Vaccination of guinea pigs using mce operon mutants of Mycobacterium tuberculosis
title_full_unstemmed Vaccination of guinea pigs using mce operon mutants of Mycobacterium tuberculosis
title_sort Vaccination of guinea pigs using mce operon mutants of Mycobacterium tuberculosis
dc.creator.none.fl_str_mv Obregón Henao, Andrés
Shanley, Crystal
Bianco, María Verónica
Cataldi, Ángel Adrián
Basaraba, Randall J.
Orme, Ian M.
Bigi, Fabiana
author Obregón Henao, Andrés
author_facet Obregón Henao, Andrés
Shanley, Crystal
Bianco, María Verónica
Cataldi, Ángel Adrián
Basaraba, Randall J.
Orme, Ian M.
Bigi, Fabiana
author_role author
author2 Shanley, Crystal
Bianco, María Verónica
Cataldi, Ángel Adrián
Basaraba, Randall J.
Orme, Ian M.
Bigi, Fabiana
author2_role author
author
author
author
author
author
dc.subject.none.fl_str_mv ATTENUATED MUTANTS
GUINEA PIG
TUBERCULOSIS
VACCINATION
topic ATTENUATED MUTANTS
GUINEA PIG
TUBERCULOSIS
VACCINATION
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.7
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv The limited efficacy of the BCG vaccine for tuberculosis, coupled with emerging information suggesting that it is poorly protective against newly emerging strains of Mycobacterium tuberculosis such as the W-Beijing isolates, makes it paramount to search for more potent alternatives. One such class of candidates is attenuated mutants derived from M. tuberculosis itself. We demonstrate here, in an initial short term assay, that mutants derived from disruption of the mce genes of the bacillus were highly protective in guinea pigs exposed by low dose aerosol infection with the virulent W-Beijing isolate SA161. This protection was demonstrated by a significant reduction in the numbers of bacilli harvested from the lungs, and dramatic improvements in lung histopathology.
Fil: Obregón Henao, Andrés. State University of Colorado - Fort Collins; Estados Unidos
Fil: Shanley, Crystal. State University of Colorado - Fort Collins; Estados Unidos
Fil: Bianco, María Verónica. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Biotecnología; Argentina
Fil: Cataldi, Ángel Adrián. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Basaraba, Randall J.. State University of Colorado - Fort Collins; Estados Unidos
Fil: Orme, Ian M.. State University of Colorado - Fort Collins; Estados Unidos
Fil: Bigi, Fabiana. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Biotecnología; Argentina
description The limited efficacy of the BCG vaccine for tuberculosis, coupled with emerging information suggesting that it is poorly protective against newly emerging strains of Mycobacterium tuberculosis such as the W-Beijing isolates, makes it paramount to search for more potent alternatives. One such class of candidates is attenuated mutants derived from M. tuberculosis itself. We demonstrate here, in an initial short term assay, that mutants derived from disruption of the mce genes of the bacillus were highly protective in guinea pigs exposed by low dose aerosol infection with the virulent W-Beijing isolate SA161. This protection was demonstrated by a significant reduction in the numbers of bacilli harvested from the lungs, and dramatic improvements in lung histopathology.
publishDate 2011
dc.date.none.fl_str_mv 2011-08
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/190048
Obregón Henao, Andrés; Shanley, Crystal; Bianco, María Verónica; Cataldi, Ángel Adrián; Basaraba, Randall J.; et al.; Vaccination of guinea pigs using mce operon mutants of Mycobacterium tuberculosis; Elsevier; Vaccine; 29; 26; 8-2011; 4302-4307
0264-410X
CONICET Digital
CONICET
url http://hdl.handle.net/11336/190048
identifier_str_mv Obregón Henao, Andrés; Shanley, Crystal; Bianco, María Verónica; Cataldi, Ángel Adrián; Basaraba, Randall J.; et al.; Vaccination of guinea pigs using mce operon mutants of Mycobacterium tuberculosis; Elsevier; Vaccine; 29; 26; 8-2011; 4302-4307
0264-410X
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S0264410X11005627
info:eu-repo/semantics/altIdentifier/doi/10.1016/j.vaccine.2011.04.027
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1846083235827679232
score 13.22299

Publicaciones similares