Paneth cell identification in the small intestine of guinea pig offsprings (Cavia porcellus)

Autores
Vásquez Cachay, María Elith; Pebe, Erika; Rodríguez Gutiérrez, José Luis; Mejía, Boris Lira; Falcón Pérez, Néstor; Zanuzzi, Carolina Natalia; Barbeito, Claudio Gustavo
Año de publicación
2014
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The aim of this study was to determine the presence, number, and morphometrical characteristics of Paneth cells (PC) in the small intestine of guinea pigs during lactation. We used 48 pups from 0 to 15 days old. Samples from small intestine were fixed in 10% buffered formaldehyde (pH 7.4) and processed for histological and morphometrical studies using hematoxylin and eosin (HE), Phloxine tartrazine or Masson's Trichome staining, or immunohistochemistry for lysozyme. PC were morphologically identified at day 2 using Masson's Trichome or Phloxine tartrazine stainings, and at day 4 using HE, whereas using immunohistochemistry they were recognized from birth. Morphometrical differences were found between the intestinal sections at each age studied, and within each section during the first weeks of life. In all developmental stage, the highest number of PC was observed in the duodenum of 13 days old guinea pigs. Our results confirm the presence of PC in the small intestine of guinea pigs from birth.
Fil: Vásquez Cachay, María Elith. Universidad Nacional Mayor de San Marcos; Perú
Fil: Pebe, Erika. Universidad Nacional Mayor de San Marcos; Perú
Fil: Rodríguez Gutiérrez, José Luis. Universidad Nacional Mayor de San Marcos; Perú
Fil: Mejía, Boris Lira. Universidad Nacional Mayor de San Marcos; Perú
Fil: Falcón Pérez, Néstor. Universidad Cayetano Heredia; Perú
Fil: Zanuzzi, Carolina Natalia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata; Argentina. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Departamento de Ciencias Básicas. Cátedra de Histología y Embriología; Argentina
Fil: Barbeito, Claudio Gustavo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata; Argentina. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Departamento de Ciencias Básicas. Cátedra de Histología y Embriología; Argentina
Materia
Guinea Pig
Immunohistochemistry
Lactation
Lysozyme
Paneth Cells
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/79183
Acceder
id CONICETDig_f782a50993f5801f7830fc4685aac105
oai_identifier_str oai:ri.conicet.gov.ar:11336/79183
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Paneth cell identification in the small intestine of guinea pig offsprings (Cavia porcellus)Vásquez Cachay, María ElithPebe, ErikaRodríguez Gutiérrez, José LuisMejía, Boris LiraFalcón Pérez, NéstorZanuzzi, Carolina NataliaBarbeito, Claudio GustavoGuinea PigImmunohistochemistryLactationLysozymePaneth Cellshttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1https://purl.org/becyt/ford/3.1https://purl.org/becyt/ford/3https://purl.org/becyt/ford/4.3https://purl.org/becyt/ford/4The aim of this study was to determine the presence, number, and morphometrical characteristics of Paneth cells (PC) in the small intestine of guinea pigs during lactation. We used 48 pups from 0 to 15 days old. Samples from small intestine were fixed in 10% buffered formaldehyde (pH 7.4) and processed for histological and morphometrical studies using hematoxylin and eosin (HE), Phloxine tartrazine or Masson's Trichome staining, or immunohistochemistry for lysozyme. PC were morphologically identified at day 2 using Masson's Trichome or Phloxine tartrazine stainings, and at day 4 using HE, whereas using immunohistochemistry they were recognized from birth. Morphometrical differences were found between the intestinal sections at each age studied, and within each section during the first weeks of life. In all developmental stage, the highest number of PC was observed in the duodenum of 13 days old guinea pigs. Our results confirm the presence of PC in the small intestine of guinea pigs from birth.Fil: Vásquez Cachay, María Elith. Universidad Nacional Mayor de San Marcos; PerúFil: Pebe, Erika. Universidad Nacional Mayor de San Marcos; PerúFil: Rodríguez Gutiérrez, José Luis. Universidad Nacional Mayor de San Marcos; PerúFil: Mejía, Boris Lira. Universidad Nacional Mayor de San Marcos; PerúFil: Falcón Pérez, Néstor. Universidad Cayetano Heredia; PerúFil: Zanuzzi, Carolina Natalia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata; Argentina. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Departamento de Ciencias Básicas. Cátedra de Histología y Embriología; ArgentinaFil: Barbeito, Claudio Gustavo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata; Argentina. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Departamento de Ciencias Básicas. Cátedra de Histología y Embriología; ArgentinaWiley-liss, Div John Wiley & Sons Inc2014-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/79183Vásquez Cachay, María Elith; Pebe, Erika; Rodríguez Gutiérrez, José Luis; Mejía, Boris Lira; Falcón Pérez, Néstor; et al.; Paneth cell identification in the small intestine of guinea pig offsprings (Cavia porcellus); Wiley-liss, Div John Wiley & Sons Inc; Anatomical Record-Advances in Integrative Anatomy and Evolutionary Biology; 297; 5; 1-2014; 856-8631932-8486CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1002/ar.22890info:eu-repo/semantics/altIdentifier/url/https://onlinelibrary.wiley.com/doi/abs/10.1002/ar.22890info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-29T12:55:31Zoai:ri.conicet.gov.ar:11336/79183instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-29 12:55:32.266CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Paneth cell identification in the small intestine of guinea pig offsprings (Cavia porcellus)
title Paneth cell identification in the small intestine of guinea pig offsprings (Cavia porcellus)
spellingShingle Paneth cell identification in the small intestine of guinea pig offsprings (Cavia porcellus)
Vásquez Cachay, María Elith
Guinea Pig
Immunohistochemistry
Lactation
Lysozyme
Paneth Cells
title_short Paneth cell identification in the small intestine of guinea pig offsprings (Cavia porcellus)
title_full Paneth cell identification in the small intestine of guinea pig offsprings (Cavia porcellus)
title_fullStr Paneth cell identification in the small intestine of guinea pig offsprings (Cavia porcellus)
title_full_unstemmed Paneth cell identification in the small intestine of guinea pig offsprings (Cavia porcellus)
title_sort Paneth cell identification in the small intestine of guinea pig offsprings (Cavia porcellus)
dc.creator.none.fl_str_mv Vásquez Cachay, María Elith
Pebe, Erika
Rodríguez Gutiérrez, José Luis
Mejía, Boris Lira
Falcón Pérez, Néstor
Zanuzzi, Carolina Natalia
Barbeito, Claudio Gustavo
author Vásquez Cachay, María Elith
author_facet Vásquez Cachay, María Elith
Pebe, Erika
Rodríguez Gutiérrez, José Luis
Mejía, Boris Lira
Falcón Pérez, Néstor
Zanuzzi, Carolina Natalia
Barbeito, Claudio Gustavo
author_role author
author2 Pebe, Erika
Rodríguez Gutiérrez, José Luis
Mejía, Boris Lira
Falcón Pérez, Néstor
Zanuzzi, Carolina Natalia
Barbeito, Claudio Gustavo
author2_role author
author
author
author
author
author
dc.subject.none.fl_str_mv Guinea Pig
Immunohistochemistry
Lactation
Lysozyme
Paneth Cells
topic Guinea Pig
Immunohistochemistry
Lactation
Lysozyme
Paneth Cells
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
https://purl.org/becyt/ford/3.1
https://purl.org/becyt/ford/3
https://purl.org/becyt/ford/4.3
https://purl.org/becyt/ford/4
dc.description.none.fl_txt_mv The aim of this study was to determine the presence, number, and morphometrical characteristics of Paneth cells (PC) in the small intestine of guinea pigs during lactation. We used 48 pups from 0 to 15 days old. Samples from small intestine were fixed in 10% buffered formaldehyde (pH 7.4) and processed for histological and morphometrical studies using hematoxylin and eosin (HE), Phloxine tartrazine or Masson's Trichome staining, or immunohistochemistry for lysozyme. PC were morphologically identified at day 2 using Masson's Trichome or Phloxine tartrazine stainings, and at day 4 using HE, whereas using immunohistochemistry they were recognized from birth. Morphometrical differences were found between the intestinal sections at each age studied, and within each section during the first weeks of life. In all developmental stage, the highest number of PC was observed in the duodenum of 13 days old guinea pigs. Our results confirm the presence of PC in the small intestine of guinea pigs from birth.
Fil: Vásquez Cachay, María Elith. Universidad Nacional Mayor de San Marcos; Perú
Fil: Pebe, Erika. Universidad Nacional Mayor de San Marcos; Perú
Fil: Rodríguez Gutiérrez, José Luis. Universidad Nacional Mayor de San Marcos; Perú
Fil: Mejía, Boris Lira. Universidad Nacional Mayor de San Marcos; Perú
Fil: Falcón Pérez, Néstor. Universidad Cayetano Heredia; Perú
Fil: Zanuzzi, Carolina Natalia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata; Argentina. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Departamento de Ciencias Básicas. Cátedra de Histología y Embriología; Argentina
Fil: Barbeito, Claudio Gustavo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata; Argentina. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Departamento de Ciencias Básicas. Cátedra de Histología y Embriología; Argentina
description The aim of this study was to determine the presence, number, and morphometrical characteristics of Paneth cells (PC) in the small intestine of guinea pigs during lactation. We used 48 pups from 0 to 15 days old. Samples from small intestine were fixed in 10% buffered formaldehyde (pH 7.4) and processed for histological and morphometrical studies using hematoxylin and eosin (HE), Phloxine tartrazine or Masson's Trichome staining, or immunohistochemistry for lysozyme. PC were morphologically identified at day 2 using Masson's Trichome or Phloxine tartrazine stainings, and at day 4 using HE, whereas using immunohistochemistry they were recognized from birth. Morphometrical differences were found between the intestinal sections at each age studied, and within each section during the first weeks of life. In all developmental stage, the highest number of PC was observed in the duodenum of 13 days old guinea pigs. Our results confirm the presence of PC in the small intestine of guinea pigs from birth.
publishDate 2014
dc.date.none.fl_str_mv 2014-01
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/79183
Vásquez Cachay, María Elith; Pebe, Erika; Rodríguez Gutiérrez, José Luis; Mejía, Boris Lira; Falcón Pérez, Néstor; et al.; Paneth cell identification in the small intestine of guinea pig offsprings (Cavia porcellus); Wiley-liss, Div John Wiley & Sons Inc; Anatomical Record-Advances in Integrative Anatomy and Evolutionary Biology; 297; 5; 1-2014; 856-863
1932-8486
CONICET Digital
CONICET
url http://hdl.handle.net/11336/79183
identifier_str_mv Vásquez Cachay, María Elith; Pebe, Erika; Rodríguez Gutiérrez, José Luis; Mejía, Boris Lira; Falcón Pérez, Néstor; et al.; Paneth cell identification in the small intestine of guinea pig offsprings (Cavia porcellus); Wiley-liss, Div John Wiley & Sons Inc; Anatomical Record-Advances in Integrative Anatomy and Evolutionary Biology; 297; 5; 1-2014; 856-863
1932-8486
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1002/ar.22890
info:eu-repo/semantics/altIdentifier/url/https://onlinelibrary.wiley.com/doi/abs/10.1002/ar.22890
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Wiley-liss, Div John Wiley & Sons Inc
publisher.none.fl_str_mv Wiley-liss, Div John Wiley & Sons Inc
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.10058

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