Low-dose estrogen is as effective as high-dose treatment in rats with postmenopausal hypertension

Autores
Campos, Cristina; Sartorio, Carmen L.; Casali, Karina R.; Fernandes, Rafael O.; Llesuy, Susana Francisca; Rosa Araujo, Alex S. da; Belló Klein, Adriane; Rigatto, Katya Vianna
Año de publicación
2014
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
This study was conducted to test the hypothesis that 17β-estradiol therapy improves redox balance by decreasing reactive oxygen species production and increasing nitric oxide (NO) bioavailability, favoring Akt pathway activation and resulting in a better autonomic vascular control. Ovariectomized female Wistar rats were divided into 4 groups: (1) vehicle (VL) and animals treated with a pellet of 17β-estradiol for 21 days; (2) low dose (LE; 0.05 mg); (3) medium dose (ME; 0.2 mg); and (4) high dose (HE; 0.5 mg). Arterial pressure and its sympathetic nervous system modulation were evaluated by spectral analysis. Nitric oxide synthase and NADPH oxidase (Nox) activities, H2O2 concentration, redox status (GSH/GSSG), protein expression of Trx-1 and p-Akt/Akt were evaluated in the aorta, whereas NO metabolites were measured in the serum. Estrogen-treated groups showed a significant decrease in arterial pressure and sympathetic vascular drive. Redox status was significantly improved and NADPH oxidase and H2O2 were decreased in all estrogen-treated groups. Estrogen also induced an enhancement in NO metabolites, nitric oxide synthase activity, and Akt phosphorylation. This study demonstrated that estrogen treatment to ovariectomized rats induced cardioprotection, which was evidenced by reduced blood pressure variability and vascular sympathetic drive. These effects were associated with an improved redox balance and Akt activation, resulting in an enhanced NO bioavailability.
Fil: Campos, Cristina. Universidade Federal do Rio Grande do Sul; Brasil
Fil: Sartorio, Carmen L.. Universidade Federal do Rio Grande do Sul; Brasil
Fil: Casali, Karina R.. Universidade Federal do Rio Grande do Sul; Brasil
Fil: Fernandes, Rafael O.. Universidade Federal do Rio Grande do Sul; Brasil
Fil: Llesuy, Susana Francisca. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Bioquímica y Medicina Molecular. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Bioquímica y Medicina Molecular; Argentina
Fil: Rosa Araujo, Alex S. da. Universidade Federal do Rio Grande do Sul; Brasil
Fil: Belló Klein, Adriane. Universidade Federal do Rio Grande do Sul; Brasil
Fil: Rigatto, Katya Vianna. Universidade Federal de Ciências da Saúde de Porto Alegre; Brasil
Materia
Estrogen Therapy,
Ovariectomized Rats
Blood Pressure Variability
Hydrogen Peroxide
Thioredoxin
Nadph Oxidase
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/30441
Acceder
id CONICETDig_e96de4ebef443aaf1bb6794c6d26a480
oai_identifier_str oai:ri.conicet.gov.ar:11336/30441
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Low-dose estrogen is as effective as high-dose treatment in rats with postmenopausal hypertensionCampos, CristinaSartorio, Carmen L.Casali, Karina R.Fernandes, Rafael O.Llesuy, Susana FranciscaRosa Araujo, Alex S. daBelló Klein, AdrianeRigatto, Katya ViannaEstrogen Therapy,Ovariectomized RatsBlood Pressure VariabilityHydrogen PeroxideThioredoxinNadph Oxidasehttps://purl.org/becyt/ford/3.3https://purl.org/becyt/ford/3This study was conducted to test the hypothesis that 17β-estradiol therapy improves redox balance by decreasing reactive oxygen species production and increasing nitric oxide (NO) bioavailability, favoring Akt pathway activation and resulting in a better autonomic vascular control. Ovariectomized female Wistar rats were divided into 4 groups: (1) vehicle (VL) and animals treated with a pellet of 17β-estradiol for 21 days; (2) low dose (LE; 0.05 mg); (3) medium dose (ME; 0.2 mg); and (4) high dose (HE; 0.5 mg). Arterial pressure and its sympathetic nervous system modulation were evaluated by spectral analysis. Nitric oxide synthase and NADPH oxidase (Nox) activities, H2O2 concentration, redox status (GSH/GSSG), protein expression of Trx-1 and p-Akt/Akt were evaluated in the aorta, whereas NO metabolites were measured in the serum. Estrogen-treated groups showed a significant decrease in arterial pressure and sympathetic vascular drive. Redox status was significantly improved and NADPH oxidase and H2O2 were decreased in all estrogen-treated groups. Estrogen also induced an enhancement in NO metabolites, nitric oxide synthase activity, and Akt phosphorylation. This study demonstrated that estrogen treatment to ovariectomized rats induced cardioprotection, which was evidenced by reduced blood pressure variability and vascular sympathetic drive. These effects were associated with an improved redox balance and Akt activation, resulting in an enhanced NO bioavailability.Fil: Campos, Cristina. Universidade Federal do Rio Grande do Sul; BrasilFil: Sartorio, Carmen L.. Universidade Federal do Rio Grande do Sul; BrasilFil: Casali, Karina R.. Universidade Federal do Rio Grande do Sul; BrasilFil: Fernandes, Rafael O.. Universidade Federal do Rio Grande do Sul; BrasilFil: Llesuy, Susana Francisca. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Bioquímica y Medicina Molecular. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Bioquímica y Medicina Molecular; ArgentinaFil: Rosa Araujo, Alex S. da. Universidade Federal do Rio Grande do Sul; BrasilFil: Belló Klein, Adriane. Universidade Federal do Rio Grande do Sul; BrasilFil: Rigatto, Katya Vianna. Universidade Federal de Ciências da Saúde de Porto Alegre; BrasilLippincott Williams2014-02info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/30441Campos, Cristina; Sartorio, Carmen L.; Casali, Karina R.; Fernandes, Rafael O.; Llesuy, Susana Francisca; et al.; Low-dose estrogen is as effective as high-dose treatment in rats with postmenopausal hypertension; Lippincott Williams; Journal Of Cardiovascular Pharmacology; 63; 2; 2-2014; 144-1510160-2446CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1097/FJC.0000000000000034info:eu-repo/semantics/altIdentifier/url/https://insights.ovid.com/pubmed?pmid=24157955info:eu-repo/semantics/altIdentifier/url/http://journals.lww.com/cardiovascularpharm/pages/articleviewer.aspx?year=2014&issue=02000&article=00008&type=abstractinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T09:45:09Zoai:ri.conicet.gov.ar:11336/30441instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 09:45:09.458CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Low-dose estrogen is as effective as high-dose treatment in rats with postmenopausal hypertension
title Low-dose estrogen is as effective as high-dose treatment in rats with postmenopausal hypertension
spellingShingle Low-dose estrogen is as effective as high-dose treatment in rats with postmenopausal hypertension
Campos, Cristina
Estrogen Therapy,
Ovariectomized Rats
Blood Pressure Variability
Hydrogen Peroxide
Thioredoxin
Nadph Oxidase
title_short Low-dose estrogen is as effective as high-dose treatment in rats with postmenopausal hypertension
title_full Low-dose estrogen is as effective as high-dose treatment in rats with postmenopausal hypertension
title_fullStr Low-dose estrogen is as effective as high-dose treatment in rats with postmenopausal hypertension
title_full_unstemmed Low-dose estrogen is as effective as high-dose treatment in rats with postmenopausal hypertension
title_sort Low-dose estrogen is as effective as high-dose treatment in rats with postmenopausal hypertension
dc.creator.none.fl_str_mv Campos, Cristina
Sartorio, Carmen L.
Casali, Karina R.
Fernandes, Rafael O.
Llesuy, Susana Francisca
Rosa Araujo, Alex S. da
Belló Klein, Adriane
Rigatto, Katya Vianna
author Campos, Cristina
author_facet Campos, Cristina
Sartorio, Carmen L.
Casali, Karina R.
Fernandes, Rafael O.
Llesuy, Susana Francisca
Rosa Araujo, Alex S. da
Belló Klein, Adriane
Rigatto, Katya Vianna
author_role author
author2 Sartorio, Carmen L.
Casali, Karina R.
Fernandes, Rafael O.
Llesuy, Susana Francisca
Rosa Araujo, Alex S. da
Belló Klein, Adriane
Rigatto, Katya Vianna
author2_role author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Estrogen Therapy,
Ovariectomized Rats
Blood Pressure Variability
Hydrogen Peroxide
Thioredoxin
Nadph Oxidase
topic Estrogen Therapy,
Ovariectomized Rats
Blood Pressure Variability
Hydrogen Peroxide
Thioredoxin
Nadph Oxidase
purl_subject.fl_str_mv https://purl.org/becyt/ford/3.3
https://purl.org/becyt/ford/3
dc.description.none.fl_txt_mv This study was conducted to test the hypothesis that 17β-estradiol therapy improves redox balance by decreasing reactive oxygen species production and increasing nitric oxide (NO) bioavailability, favoring Akt pathway activation and resulting in a better autonomic vascular control. Ovariectomized female Wistar rats were divided into 4 groups: (1) vehicle (VL) and animals treated with a pellet of 17β-estradiol for 21 days; (2) low dose (LE; 0.05 mg); (3) medium dose (ME; 0.2 mg); and (4) high dose (HE; 0.5 mg). Arterial pressure and its sympathetic nervous system modulation were evaluated by spectral analysis. Nitric oxide synthase and NADPH oxidase (Nox) activities, H2O2 concentration, redox status (GSH/GSSG), protein expression of Trx-1 and p-Akt/Akt were evaluated in the aorta, whereas NO metabolites were measured in the serum. Estrogen-treated groups showed a significant decrease in arterial pressure and sympathetic vascular drive. Redox status was significantly improved and NADPH oxidase and H2O2 were decreased in all estrogen-treated groups. Estrogen also induced an enhancement in NO metabolites, nitric oxide synthase activity, and Akt phosphorylation. This study demonstrated that estrogen treatment to ovariectomized rats induced cardioprotection, which was evidenced by reduced blood pressure variability and vascular sympathetic drive. These effects were associated with an improved redox balance and Akt activation, resulting in an enhanced NO bioavailability.
Fil: Campos, Cristina. Universidade Federal do Rio Grande do Sul; Brasil
Fil: Sartorio, Carmen L.. Universidade Federal do Rio Grande do Sul; Brasil
Fil: Casali, Karina R.. Universidade Federal do Rio Grande do Sul; Brasil
Fil: Fernandes, Rafael O.. Universidade Federal do Rio Grande do Sul; Brasil
Fil: Llesuy, Susana Francisca. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Bioquímica y Medicina Molecular. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Bioquímica y Medicina Molecular; Argentina
Fil: Rosa Araujo, Alex S. da. Universidade Federal do Rio Grande do Sul; Brasil
Fil: Belló Klein, Adriane. Universidade Federal do Rio Grande do Sul; Brasil
Fil: Rigatto, Katya Vianna. Universidade Federal de Ciências da Saúde de Porto Alegre; Brasil
description This study was conducted to test the hypothesis that 17β-estradiol therapy improves redox balance by decreasing reactive oxygen species production and increasing nitric oxide (NO) bioavailability, favoring Akt pathway activation and resulting in a better autonomic vascular control. Ovariectomized female Wistar rats were divided into 4 groups: (1) vehicle (VL) and animals treated with a pellet of 17β-estradiol for 21 days; (2) low dose (LE; 0.05 mg); (3) medium dose (ME; 0.2 mg); and (4) high dose (HE; 0.5 mg). Arterial pressure and its sympathetic nervous system modulation were evaluated by spectral analysis. Nitric oxide synthase and NADPH oxidase (Nox) activities, H2O2 concentration, redox status (GSH/GSSG), protein expression of Trx-1 and p-Akt/Akt were evaluated in the aorta, whereas NO metabolites were measured in the serum. Estrogen-treated groups showed a significant decrease in arterial pressure and sympathetic vascular drive. Redox status was significantly improved and NADPH oxidase and H2O2 were decreased in all estrogen-treated groups. Estrogen also induced an enhancement in NO metabolites, nitric oxide synthase activity, and Akt phosphorylation. This study demonstrated that estrogen treatment to ovariectomized rats induced cardioprotection, which was evidenced by reduced blood pressure variability and vascular sympathetic drive. These effects were associated with an improved redox balance and Akt activation, resulting in an enhanced NO bioavailability.
publishDate 2014
dc.date.none.fl_str_mv 2014-02
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/30441
Campos, Cristina; Sartorio, Carmen L.; Casali, Karina R.; Fernandes, Rafael O.; Llesuy, Susana Francisca; et al.; Low-dose estrogen is as effective as high-dose treatment in rats with postmenopausal hypertension; Lippincott Williams; Journal Of Cardiovascular Pharmacology; 63; 2; 2-2014; 144-151
0160-2446
CONICET Digital
CONICET
url http://hdl.handle.net/11336/30441
identifier_str_mv Campos, Cristina; Sartorio, Carmen L.; Casali, Karina R.; Fernandes, Rafael O.; Llesuy, Susana Francisca; et al.; Low-dose estrogen is as effective as high-dose treatment in rats with postmenopausal hypertension; Lippincott Williams; Journal Of Cardiovascular Pharmacology; 63; 2; 2-2014; 144-151
0160-2446
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1097/FJC.0000000000000034
info:eu-repo/semantics/altIdentifier/url/https://insights.ovid.com/pubmed?pmid=24157955
info:eu-repo/semantics/altIdentifier/url/http://journals.lww.com/cardiovascularpharm/pages/articleviewer.aspx?year=2014&issue=02000&article=00008&type=abstract
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Lippincott Williams
publisher.none.fl_str_mv Lippincott Williams
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1844613419555094528
score 13.070432

Publicaciones similares