Structural features of ultradeformable archaeosomes for topical delivery of ovalbumin

Autores
Carrer, Dolores Catalina; Higa, Leticia Herminia; Defain Tesoriero, Maria Victoria; Morilla, María José; Roncaglia, Diana Inés; Romero, Eder Lilia
Año de publicación
2014
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The ultradeformable archaeosomes (UDA, made of total polar archaeolipids (TPA) extracted from the extreme halophile archaea Halorubrum tebenquichense:soybean phosphatidylcholine (SPC):sodium cholate (NaChol), 3:3:1 w:w), are promising topical adjuvants showing high deformability, an essential property for intact skin penetration up to the viable epidermis/dermis. To gain insights on UDA structure, the interactions between TPA, SPC and the edge activator NaChol, were assessed by electrospray ionization mass spectroscopy (ESI-MS) and confocal fluorescence microscopy of giant unilamellar vesicles (GUV). The non covalent heterodimers NaChol–SPC, NaChol–phosphatidylglycerophosphate methyl ether (PGPMe), NaChol–sulfated diglycosyl diphytanyl–glycerol diether (SDGD5) and SPC–PGPMe detected in the gas phase by ESI-MS after direct infusion of UDA, together with the homogeneous partition of FASTDiO and DiIC18 in GUV suggested that in these proportions, lipids and NaChol were miscible. We propose therefore, a model where in UDA the SPC diluted sufficient enough in the rich PGPMe TPA, so as to the low lateral mobility of molecules (typical of rich in PGPMe bilayers) was no longer experienced. We also found that 50 μm deep within in vitro human skin canyons, the fluorescence of Alexa fluor 647-ovalbumin in UDL was ∼1.5 folds higher than in UDA, indicating a potential steric hindrance of the voluminous structure of PGPMe UDA bilayer, to the penetration of a particulate cargo such as the 7 nm diameter ovalbumin. According to these observations, a further reduction in PGPMe – a lipid playing no immune role – content could help to improve the performance of UDA as topical adjuvants.
Fil: Carrer, Dolores Catalina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigación Médica Mercedes y Martín Ferreyra. Universidad Nacional de Córdoba. Instituto de Investigación Médica Mercedes y Martín Ferreyra; Argentina
Fil: Higa, Leticia Herminia. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de Quilmes. Departamento de Ciencia y Tecnología; Argentina
Fil: Defain Tesoriero, Maria Victoria. Universidad Nacional de Quilmes. Departamento de Ciencia y Tecnología; Argentina. Instituto Nacional de Tecnología Industrial; Argentina
Fil: Morilla, María José. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de Quilmes. Departamento de Ciencia y Tecnología; Argentina
Fil: Roncaglia, Diana Inés. Universidad Nacional de Quilmes. Departamento de Ciencia y Tecnología; Argentina
Fil: Romero, Eder Lilia. Universidad Nacional de Quilmes. Departamento de Ciencia y Tecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Materia
Penetration
Skin
Ultradeformable Archaeosomes
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/31637
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/31637
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Structural features of ultradeformable archaeosomes for topical delivery of ovalbuminCarrer, Dolores CatalinaHiga, Leticia HerminiaDefain Tesoriero, Maria VictoriaMorilla, María JoséRoncaglia, Diana InésRomero, Eder LiliaPenetrationSkinUltradeformable Archaeosomeshttps://purl.org/becyt/ford/2.10https://purl.org/becyt/ford/2The ultradeformable archaeosomes (UDA, made of total polar archaeolipids (TPA) extracted from the extreme halophile archaea Halorubrum tebenquichense:soybean phosphatidylcholine (SPC):sodium cholate (NaChol), 3:3:1 w:w), are promising topical adjuvants showing high deformability, an essential property for intact skin penetration up to the viable epidermis/dermis. To gain insights on UDA structure, the interactions between TPA, SPC and the edge activator NaChol, were assessed by electrospray ionization mass spectroscopy (ESI-MS) and confocal fluorescence microscopy of giant unilamellar vesicles (GUV). The non covalent heterodimers NaChol–SPC, NaChol–phosphatidylglycerophosphate methyl ether (PGPMe), NaChol–sulfated diglycosyl diphytanyl–glycerol diether (SDGD5) and SPC–PGPMe detected in the gas phase by ESI-MS after direct infusion of UDA, together with the homogeneous partition of FASTDiO and DiIC18 in GUV suggested that in these proportions, lipids and NaChol were miscible. We propose therefore, a model where in UDA the SPC diluted sufficient enough in the rich PGPMe TPA, so as to the low lateral mobility of molecules (typical of rich in PGPMe bilayers) was no longer experienced. We also found that 50 μm deep within in vitro human skin canyons, the fluorescence of Alexa fluor 647-ovalbumin in UDL was ∼1.5 folds higher than in UDA, indicating a potential steric hindrance of the voluminous structure of PGPMe UDA bilayer, to the penetration of a particulate cargo such as the 7 nm diameter ovalbumin. According to these observations, a further reduction in PGPMe – a lipid playing no immune role – content could help to improve the performance of UDA as topical adjuvants.Fil: Carrer, Dolores Catalina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigación Médica Mercedes y Martín Ferreyra. Universidad Nacional de Córdoba. Instituto de Investigación Médica Mercedes y Martín Ferreyra; ArgentinaFil: Higa, Leticia Herminia. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de Quilmes. Departamento de Ciencia y Tecnología; ArgentinaFil: Defain Tesoriero, Maria Victoria. Universidad Nacional de Quilmes. Departamento de Ciencia y Tecnología; Argentina. Instituto Nacional de Tecnología Industrial; ArgentinaFil: Morilla, María José. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de Quilmes. Departamento de Ciencia y Tecnología; ArgentinaFil: Roncaglia, Diana Inés. Universidad Nacional de Quilmes. Departamento de Ciencia y Tecnología; ArgentinaFil: Romero, Eder Lilia. Universidad Nacional de Quilmes. Departamento de Ciencia y Tecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaElsevier Science2014-05info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/31637Romero, Eder Lilia; Roncaglia, Diana Inés; Morilla, María José; Defain Tesoriero, Maria Victoria; Higa, Leticia Herminia; Carrer, Dolores Catalina; et al.; Structural features of ultradeformable archaeosomes for topical delivery of ovalbumin; Elsevier Science; Colloids and Surfaces B: Biointerfaces; 121; 5-2014; 281-2890927-7765CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1016/j.colsurfb.2014.05.015info:eu-repo/semantics/altIdentifier/url/http://www.sciencedirect.com/science/article/pii/S0927776514002501info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T09:56:24Zoai:ri.conicet.gov.ar:11336/31637instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 09:56:24.972CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Structural features of ultradeformable archaeosomes for topical delivery of ovalbumin
title Structural features of ultradeformable archaeosomes for topical delivery of ovalbumin
spellingShingle Structural features of ultradeformable archaeosomes for topical delivery of ovalbumin
Carrer, Dolores Catalina
Penetration
Skin
Ultradeformable Archaeosomes
title_short Structural features of ultradeformable archaeosomes for topical delivery of ovalbumin
title_full Structural features of ultradeformable archaeosomes for topical delivery of ovalbumin
title_fullStr Structural features of ultradeformable archaeosomes for topical delivery of ovalbumin
title_full_unstemmed Structural features of ultradeformable archaeosomes for topical delivery of ovalbumin
title_sort Structural features of ultradeformable archaeosomes for topical delivery of ovalbumin
dc.creator.none.fl_str_mv Carrer, Dolores Catalina
Higa, Leticia Herminia
Defain Tesoriero, Maria Victoria
Morilla, María José
Roncaglia, Diana Inés
Romero, Eder Lilia
author Carrer, Dolores Catalina
author_facet Carrer, Dolores Catalina
Higa, Leticia Herminia
Defain Tesoriero, Maria Victoria
Morilla, María José
Roncaglia, Diana Inés
Romero, Eder Lilia
author_role author
author2 Higa, Leticia Herminia
Defain Tesoriero, Maria Victoria
Morilla, María José
Roncaglia, Diana Inés
Romero, Eder Lilia
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv Penetration
Skin
Ultradeformable Archaeosomes
topic Penetration
Skin
Ultradeformable Archaeosomes
purl_subject.fl_str_mv https://purl.org/becyt/ford/2.10
https://purl.org/becyt/ford/2
dc.description.none.fl_txt_mv The ultradeformable archaeosomes (UDA, made of total polar archaeolipids (TPA) extracted from the extreme halophile archaea Halorubrum tebenquichense:soybean phosphatidylcholine (SPC):sodium cholate (NaChol), 3:3:1 w:w), are promising topical adjuvants showing high deformability, an essential property for intact skin penetration up to the viable epidermis/dermis. To gain insights on UDA structure, the interactions between TPA, SPC and the edge activator NaChol, were assessed by electrospray ionization mass spectroscopy (ESI-MS) and confocal fluorescence microscopy of giant unilamellar vesicles (GUV). The non covalent heterodimers NaChol–SPC, NaChol–phosphatidylglycerophosphate methyl ether (PGPMe), NaChol–sulfated diglycosyl diphytanyl–glycerol diether (SDGD5) and SPC–PGPMe detected in the gas phase by ESI-MS after direct infusion of UDA, together with the homogeneous partition of FASTDiO and DiIC18 in GUV suggested that in these proportions, lipids and NaChol were miscible. We propose therefore, a model where in UDA the SPC diluted sufficient enough in the rich PGPMe TPA, so as to the low lateral mobility of molecules (typical of rich in PGPMe bilayers) was no longer experienced. We also found that 50 μm deep within in vitro human skin canyons, the fluorescence of Alexa fluor 647-ovalbumin in UDL was ∼1.5 folds higher than in UDA, indicating a potential steric hindrance of the voluminous structure of PGPMe UDA bilayer, to the penetration of a particulate cargo such as the 7 nm diameter ovalbumin. According to these observations, a further reduction in PGPMe – a lipid playing no immune role – content could help to improve the performance of UDA as topical adjuvants.
Fil: Carrer, Dolores Catalina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigación Médica Mercedes y Martín Ferreyra. Universidad Nacional de Córdoba. Instituto de Investigación Médica Mercedes y Martín Ferreyra; Argentina
Fil: Higa, Leticia Herminia. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de Quilmes. Departamento de Ciencia y Tecnología; Argentina
Fil: Defain Tesoriero, Maria Victoria. Universidad Nacional de Quilmes. Departamento de Ciencia y Tecnología; Argentina. Instituto Nacional de Tecnología Industrial; Argentina
Fil: Morilla, María José. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de Quilmes. Departamento de Ciencia y Tecnología; Argentina
Fil: Roncaglia, Diana Inés. Universidad Nacional de Quilmes. Departamento de Ciencia y Tecnología; Argentina
Fil: Romero, Eder Lilia. Universidad Nacional de Quilmes. Departamento de Ciencia y Tecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
description The ultradeformable archaeosomes (UDA, made of total polar archaeolipids (TPA) extracted from the extreme halophile archaea Halorubrum tebenquichense:soybean phosphatidylcholine (SPC):sodium cholate (NaChol), 3:3:1 w:w), are promising topical adjuvants showing high deformability, an essential property for intact skin penetration up to the viable epidermis/dermis. To gain insights on UDA structure, the interactions between TPA, SPC and the edge activator NaChol, were assessed by electrospray ionization mass spectroscopy (ESI-MS) and confocal fluorescence microscopy of giant unilamellar vesicles (GUV). The non covalent heterodimers NaChol–SPC, NaChol–phosphatidylglycerophosphate methyl ether (PGPMe), NaChol–sulfated diglycosyl diphytanyl–glycerol diether (SDGD5) and SPC–PGPMe detected in the gas phase by ESI-MS after direct infusion of UDA, together with the homogeneous partition of FASTDiO and DiIC18 in GUV suggested that in these proportions, lipids and NaChol were miscible. We propose therefore, a model where in UDA the SPC diluted sufficient enough in the rich PGPMe TPA, so as to the low lateral mobility of molecules (typical of rich in PGPMe bilayers) was no longer experienced. We also found that 50 μm deep within in vitro human skin canyons, the fluorescence of Alexa fluor 647-ovalbumin in UDL was ∼1.5 folds higher than in UDA, indicating a potential steric hindrance of the voluminous structure of PGPMe UDA bilayer, to the penetration of a particulate cargo such as the 7 nm diameter ovalbumin. According to these observations, a further reduction in PGPMe – a lipid playing no immune role – content could help to improve the performance of UDA as topical adjuvants.
publishDate 2014
dc.date.none.fl_str_mv 2014-05
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/31637
Romero, Eder Lilia; Roncaglia, Diana Inés; Morilla, María José; Defain Tesoriero, Maria Victoria; Higa, Leticia Herminia; Carrer, Dolores Catalina; et al.; Structural features of ultradeformable archaeosomes for topical delivery of ovalbumin; Elsevier Science; Colloids and Surfaces B: Biointerfaces; 121; 5-2014; 281-289
0927-7765
CONICET Digital
CONICET
url http://hdl.handle.net/11336/31637
identifier_str_mv Romero, Eder Lilia; Roncaglia, Diana Inés; Morilla, María José; Defain Tesoriero, Maria Victoria; Higa, Leticia Herminia; Carrer, Dolores Catalina; et al.; Structural features of ultradeformable archaeosomes for topical delivery of ovalbumin; Elsevier Science; Colloids and Surfaces B: Biointerfaces; 121; 5-2014; 281-289
0927-7765
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1016/j.colsurfb.2014.05.015
info:eu-repo/semantics/altIdentifier/url/http://www.sciencedirect.com/science/article/pii/S0927776514002501
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Elsevier Science
publisher.none.fl_str_mv Elsevier Science
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.13397

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