A simple Streptomyces spore-based impedimetric biosensor to detect indane pesticide

Autores
López Rodriguez, Maria Lorena; Benimeli, Claudia Susana; Madrid, Rossana Elena; Giacomelli, Carla Eugenia
Año de publicación
2015
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Lindane is a pesticide potentially harmful to environment and human health and in general, its detection involves mostly tedious, expensive and time-consuming techniques. A new strategy to detect lindane can be focused on the activity of Streptomyces strain M7 (SM7), which is capable to grow in the presence of organochlorine pesticides (OCPs) as carbon source. On this regard, the aim of this work is to present an optimized design of a microbial biosensor prototype based on a SM7 spore platform to detect and quantify lindane by electrochemical impedance spectroscopy (EIS) as the transduction method. By means of this technique, the metabolic bacterial activity was evaluated by analyzing a set of electrical parameters calculated by fitting the measured impedance spectra through an equivalent electrical circuit. Activity studies were performed by incubating the SM7 spore platform with (optimized incubation conditions) and without (to be used in a practical biosensor) shaking in the presence of lindane. In all cases, bacterial activity was measured by the impedance changes produced in the cells due to lindane removal from the culture medium. This proposed label-free impedimetric biosensor is simple, cost-effective and feasible to detect changes in the lindane concentration as low as 120 μg L−1 in less than 2 days without sample pretreatment. In addition, the surface of electrodes can be reused for several different tests. This sensor prototype represents a valuable alternative for OCPs detection.
Fil: López Rodriguez, Maria Lorena. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Córdoba. Instituto de Investigaciones en Físicoquímica de Córdoba; Argentina. Universidad Nacional de Córdoba; Argentina
Fil: Benimeli, Claudia Susana. Universidad del Norte Santo Tomás de Aquino; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Tucumán. Planta Piloto de Procesos Industriales Microbiológicos (i); Argentina
Fil: Madrid, Rossana Elena. Universidad Nacional de Tucuman. Facultad de Cs.exactas y Tecnologia. Departamento de Bioingenieria; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Tucumán. Instituto Superior de Investigaciones Biológicas; Argentina
Fil: Giacomelli, Carla Eugenia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Córdoba. Instituto de Investigaciones en Físicoquímica de Córdoba; Argentina. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Departamento de Fisicoquímica; Argentina
Materia
Streptomyces
Impedimetric Biosensor
Spore-Based Platform
Lindane Pesticide
Impedance Spectroscopy
Electrochemical Detection
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/12468
Acceder
id CONICETDig_e966633b98ec63c06beee1627b711096
oai_identifier_str oai:ri.conicet.gov.ar:11336/12468
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling A simple Streptomyces spore-based impedimetric biosensor to detect indane pesticideLópez Rodriguez, Maria LorenaBenimeli, Claudia SusanaMadrid, Rossana ElenaGiacomelli, Carla EugeniaStreptomycesImpedimetric BiosensorSpore-Based PlatformLindane PesticideImpedance SpectroscopyElectrochemical Detectionhttps://purl.org/becyt/ford/1.4https://purl.org/becyt/ford/1Lindane is a pesticide potentially harmful to environment and human health and in general, its detection involves mostly tedious, expensive and time-consuming techniques. A new strategy to detect lindane can be focused on the activity of Streptomyces strain M7 (SM7), which is capable to grow in the presence of organochlorine pesticides (OCPs) as carbon source. On this regard, the aim of this work is to present an optimized design of a microbial biosensor prototype based on a SM7 spore platform to detect and quantify lindane by electrochemical impedance spectroscopy (EIS) as the transduction method. By means of this technique, the metabolic bacterial activity was evaluated by analyzing a set of electrical parameters calculated by fitting the measured impedance spectra through an equivalent electrical circuit. Activity studies were performed by incubating the SM7 spore platform with (optimized incubation conditions) and without (to be used in a practical biosensor) shaking in the presence of lindane. In all cases, bacterial activity was measured by the impedance changes produced in the cells due to lindane removal from the culture medium. This proposed label-free impedimetric biosensor is simple, cost-effective and feasible to detect changes in the lindane concentration as low as 120 μg L−1 in less than 2 days without sample pretreatment. In addition, the surface of electrodes can be reused for several different tests. This sensor prototype represents a valuable alternative for OCPs detection.Fil: López Rodriguez, Maria Lorena. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Córdoba. Instituto de Investigaciones en Físicoquímica de Córdoba; Argentina. Universidad Nacional de Córdoba; ArgentinaFil: Benimeli, Claudia Susana. Universidad del Norte Santo Tomás de Aquino; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Tucumán. Planta Piloto de Procesos Industriales Microbiológicos (i); ArgentinaFil: Madrid, Rossana Elena. Universidad Nacional de Tucuman. Facultad de Cs.exactas y Tecnologia. Departamento de Bioingenieria; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Tucumán. Instituto Superior de Investigaciones Biológicas; ArgentinaFil: Giacomelli, Carla Eugenia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Córdoba. Instituto de Investigaciones en Físicoquímica de Córdoba; Argentina. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Departamento de Fisicoquímica; ArgentinaElsevier Science2015-02info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/12468López Rodriguez, Maria Lorena; Benimeli, Claudia Susana; Madrid, Rossana Elena; Giacomelli, Carla Eugenia; A simple Streptomyces spore-based impedimetric biosensor to detect indane pesticide; Elsevier Science; Sensors And Actuators B: Chemical; 207; Part A; 2-2015; 447-4540925-4005enginfo:eu-repo/semantics/altIdentifier/doi/10.1016/j.snb.2014.10.030info:eu-repo/semantics/altIdentifier/url/http://www.sciencedirect.com/science/article/pii/S0925400514012404info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-nd/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:13:15Zoai:ri.conicet.gov.ar:11336/12468instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:13:15.499CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv A simple Streptomyces spore-based impedimetric biosensor to detect indane pesticide
title A simple Streptomyces spore-based impedimetric biosensor to detect indane pesticide
spellingShingle A simple Streptomyces spore-based impedimetric biosensor to detect indane pesticide
López Rodriguez, Maria Lorena
Streptomyces
Impedimetric Biosensor
Spore-Based Platform
Lindane Pesticide
Impedance Spectroscopy
Electrochemical Detection
title_short A simple Streptomyces spore-based impedimetric biosensor to detect indane pesticide
title_full A simple Streptomyces spore-based impedimetric biosensor to detect indane pesticide
title_fullStr A simple Streptomyces spore-based impedimetric biosensor to detect indane pesticide
title_full_unstemmed A simple Streptomyces spore-based impedimetric biosensor to detect indane pesticide
title_sort A simple Streptomyces spore-based impedimetric biosensor to detect indane pesticide
dc.creator.none.fl_str_mv López Rodriguez, Maria Lorena
Benimeli, Claudia Susana
Madrid, Rossana Elena
Giacomelli, Carla Eugenia
author López Rodriguez, Maria Lorena
author_facet López Rodriguez, Maria Lorena
Benimeli, Claudia Susana
Madrid, Rossana Elena
Giacomelli, Carla Eugenia
author_role author
author2 Benimeli, Claudia Susana
Madrid, Rossana Elena
Giacomelli, Carla Eugenia
author2_role author
author
author
dc.subject.none.fl_str_mv Streptomyces
Impedimetric Biosensor
Spore-Based Platform
Lindane Pesticide
Impedance Spectroscopy
Electrochemical Detection
topic Streptomyces
Impedimetric Biosensor
Spore-Based Platform
Lindane Pesticide
Impedance Spectroscopy
Electrochemical Detection
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.4
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Lindane is a pesticide potentially harmful to environment and human health and in general, its detection involves mostly tedious, expensive and time-consuming techniques. A new strategy to detect lindane can be focused on the activity of Streptomyces strain M7 (SM7), which is capable to grow in the presence of organochlorine pesticides (OCPs) as carbon source. On this regard, the aim of this work is to present an optimized design of a microbial biosensor prototype based on a SM7 spore platform to detect and quantify lindane by electrochemical impedance spectroscopy (EIS) as the transduction method. By means of this technique, the metabolic bacterial activity was evaluated by analyzing a set of electrical parameters calculated by fitting the measured impedance spectra through an equivalent electrical circuit. Activity studies were performed by incubating the SM7 spore platform with (optimized incubation conditions) and without (to be used in a practical biosensor) shaking in the presence of lindane. In all cases, bacterial activity was measured by the impedance changes produced in the cells due to lindane removal from the culture medium. This proposed label-free impedimetric biosensor is simple, cost-effective and feasible to detect changes in the lindane concentration as low as 120 μg L−1 in less than 2 days without sample pretreatment. In addition, the surface of electrodes can be reused for several different tests. This sensor prototype represents a valuable alternative for OCPs detection.
Fil: López Rodriguez, Maria Lorena. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Córdoba. Instituto de Investigaciones en Físicoquímica de Córdoba; Argentina. Universidad Nacional de Córdoba; Argentina
Fil: Benimeli, Claudia Susana. Universidad del Norte Santo Tomás de Aquino; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Tucumán. Planta Piloto de Procesos Industriales Microbiológicos (i); Argentina
Fil: Madrid, Rossana Elena. Universidad Nacional de Tucuman. Facultad de Cs.exactas y Tecnologia. Departamento de Bioingenieria; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Tucumán. Instituto Superior de Investigaciones Biológicas; Argentina
Fil: Giacomelli, Carla Eugenia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Córdoba. Instituto de Investigaciones en Físicoquímica de Córdoba; Argentina. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Departamento de Fisicoquímica; Argentina
description Lindane is a pesticide potentially harmful to environment and human health and in general, its detection involves mostly tedious, expensive and time-consuming techniques. A new strategy to detect lindane can be focused on the activity of Streptomyces strain M7 (SM7), which is capable to grow in the presence of organochlorine pesticides (OCPs) as carbon source. On this regard, the aim of this work is to present an optimized design of a microbial biosensor prototype based on a SM7 spore platform to detect and quantify lindane by electrochemical impedance spectroscopy (EIS) as the transduction method. By means of this technique, the metabolic bacterial activity was evaluated by analyzing a set of electrical parameters calculated by fitting the measured impedance spectra through an equivalent electrical circuit. Activity studies were performed by incubating the SM7 spore platform with (optimized incubation conditions) and without (to be used in a practical biosensor) shaking in the presence of lindane. In all cases, bacterial activity was measured by the impedance changes produced in the cells due to lindane removal from the culture medium. This proposed label-free impedimetric biosensor is simple, cost-effective and feasible to detect changes in the lindane concentration as low as 120 μg L−1 in less than 2 days without sample pretreatment. In addition, the surface of electrodes can be reused for several different tests. This sensor prototype represents a valuable alternative for OCPs detection.
publishDate 2015
dc.date.none.fl_str_mv 2015-02
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/12468
López Rodriguez, Maria Lorena; Benimeli, Claudia Susana; Madrid, Rossana Elena; Giacomelli, Carla Eugenia; A simple Streptomyces spore-based impedimetric biosensor to detect indane pesticide; Elsevier Science; Sensors And Actuators B: Chemical; 207; Part A; 2-2015; 447-454
0925-4005
url http://hdl.handle.net/11336/12468
identifier_str_mv López Rodriguez, Maria Lorena; Benimeli, Claudia Susana; Madrid, Rossana Elena; Giacomelli, Carla Eugenia; A simple Streptomyces spore-based impedimetric biosensor to detect indane pesticide; Elsevier Science; Sensors And Actuators B: Chemical; 207; Part A; 2-2015; 447-454
0925-4005
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1016/j.snb.2014.10.030
info:eu-repo/semantics/altIdentifier/url/http://www.sciencedirect.com/science/article/pii/S0925400514012404
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Elsevier Science
publisher.none.fl_str_mv Elsevier Science
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1844614047364808704
score 13.070432

Publicaciones similares