The optimization of the culture medium to design Streptomyces sp. M7 based impedimetric biosensors

Autores
López Rodriguez, Maria Lorena; Madrid, Rossana Elena; Giacomelli, Carla Eugenia
Año de publicación
2013
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Electrochemical impedance has been widely used in microbiology in detection, quantifying and identifying microorganisms. Streptomyces strain M7 (SM7) is capable to grow in the presence of organochlorine pesticides (OCPs) as carbon source, using the amino acid asparagine as nitrogen source. This bacterial growth can be valuable and hence used in an impedimetric biosensor to detect and, eventually, quantify these pesticides. Since amino acids frequently used in culture media interfere in impedance determinations, this work presents an optimized design regarding the culture medium capabilities on the impedance response of SM7 activity, as a first stage toward the development of an impedimetric biosensor to detect OCPs. With such a purpose, SM7 growth in liquid or adhered to a solid media was measured by impedance in the presence of asparagine or (NH4)2SO4 as nitrogen source and glucose as a model carbon source. Impedimetric SM7 measurements were performed in the Quantibac instrument that can be employed to evaluate the metabolic behavior of microorganisms in different culture media using two fixed frequencies. We found that SM7 used asparagine as carbon source in the absence of glucose. Since this consumption strongly contributed to the impedance signal, this is not a suitable medium to be used with an impedimetric biosensor. On the other hand, (NH4)2SO4 appears as an ideal medium because it supports SM7 growth in liquid and solid media without interfering the impedimetric detection. This simple and cost-effective technique combined with a spore-based platform is a promising strategy to design OCPs bacteria-based biosensors.
Fil: López Rodriguez, Maria Lorena. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones en Físico-química de Córdoba. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Instituto de Investigaciones en Físico-química de Córdoba; Argentina
Fil: Madrid, Rossana Elena. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto Superior de Investigaciones Biológicas. Universidad Nacional de Tucumán. Instituto Superior de Investigaciones Biológicas; Argentina
Fil: Giacomelli, Carla Eugenia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones en Físico-química de Córdoba. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Instituto de Investigaciones en Físico-química de Córdoba; Argentina
Materia
Streptomyces
Carbon Source
Nitrogen Source
Impedance
Spore-Based Platform
Organochlorine Pesticides
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/30312
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/30312
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling The optimization of the culture medium to design Streptomyces sp. M7 based impedimetric biosensorsLópez Rodriguez, Maria LorenaMadrid, Rossana ElenaGiacomelli, Carla EugeniaStreptomycesCarbon SourceNitrogen SourceImpedanceSpore-Based PlatformOrganochlorine Pesticideshttps://purl.org/becyt/ford/2.8https://purl.org/becyt/ford/2Electrochemical impedance has been widely used in microbiology in detection, quantifying and identifying microorganisms. Streptomyces strain M7 (SM7) is capable to grow in the presence of organochlorine pesticides (OCPs) as carbon source, using the amino acid asparagine as nitrogen source. This bacterial growth can be valuable and hence used in an impedimetric biosensor to detect and, eventually, quantify these pesticides. Since amino acids frequently used in culture media interfere in impedance determinations, this work presents an optimized design regarding the culture medium capabilities on the impedance response of SM7 activity, as a first stage toward the development of an impedimetric biosensor to detect OCPs. With such a purpose, SM7 growth in liquid or adhered to a solid media was measured by impedance in the presence of asparagine or (NH4)2SO4 as nitrogen source and glucose as a model carbon source. Impedimetric SM7 measurements were performed in the Quantibac instrument that can be employed to evaluate the metabolic behavior of microorganisms in different culture media using two fixed frequencies. We found that SM7 used asparagine as carbon source in the absence of glucose. Since this consumption strongly contributed to the impedance signal, this is not a suitable medium to be used with an impedimetric biosensor. On the other hand, (NH4)2SO4 appears as an ideal medium because it supports SM7 growth in liquid and solid media without interfering the impedimetric detection. This simple and cost-effective technique combined with a spore-based platform is a promising strategy to design OCPs bacteria-based biosensors.Fil: López Rodriguez, Maria Lorena. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones en Físico-química de Córdoba. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Instituto de Investigaciones en Físico-química de Córdoba; ArgentinaFil: Madrid, Rossana Elena. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto Superior de Investigaciones Biológicas. Universidad Nacional de Tucumán. Instituto Superior de Investigaciones Biológicas; ArgentinaFil: Giacomelli, Carla Eugenia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones en Físico-química de Córdoba. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Instituto de Investigaciones en Físico-química de Córdoba; ArgentinaElsevier Science2013-12info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/30312López Rodriguez, Maria Lorena; Madrid, Rossana Elena; Giacomelli, Carla Eugenia; The optimization of the culture medium to design Streptomyces sp. M7 based impedimetric biosensors; Elsevier Science; Sensors and Actuators B: Chemical; 193; 12-2013; 230-2370925-4005CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1016/j.snb.2013.11.066info:eu-repo/semantics/altIdentifier/url/http://www.sciencedirect.com/science/article/pii/S0925400513014160info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-nd/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:02:12Zoai:ri.conicet.gov.ar:11336/30312instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:02:13.216CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv The optimization of the culture medium to design Streptomyces sp. M7 based impedimetric biosensors
title The optimization of the culture medium to design Streptomyces sp. M7 based impedimetric biosensors
spellingShingle The optimization of the culture medium to design Streptomyces sp. M7 based impedimetric biosensors
López Rodriguez, Maria Lorena
Streptomyces
Carbon Source
Nitrogen Source
Impedance
Spore-Based Platform
Organochlorine Pesticides
title_short The optimization of the culture medium to design Streptomyces sp. M7 based impedimetric biosensors
title_full The optimization of the culture medium to design Streptomyces sp. M7 based impedimetric biosensors
title_fullStr The optimization of the culture medium to design Streptomyces sp. M7 based impedimetric biosensors
title_full_unstemmed The optimization of the culture medium to design Streptomyces sp. M7 based impedimetric biosensors
title_sort The optimization of the culture medium to design Streptomyces sp. M7 based impedimetric biosensors
dc.creator.none.fl_str_mv López Rodriguez, Maria Lorena
Madrid, Rossana Elena
Giacomelli, Carla Eugenia
author López Rodriguez, Maria Lorena
author_facet López Rodriguez, Maria Lorena
Madrid, Rossana Elena
Giacomelli, Carla Eugenia
author_role author
author2 Madrid, Rossana Elena
Giacomelli, Carla Eugenia
author2_role author
author
dc.subject.none.fl_str_mv Streptomyces
Carbon Source
Nitrogen Source
Impedance
Spore-Based Platform
Organochlorine Pesticides
topic Streptomyces
Carbon Source
Nitrogen Source
Impedance
Spore-Based Platform
Organochlorine Pesticides
purl_subject.fl_str_mv https://purl.org/becyt/ford/2.8
https://purl.org/becyt/ford/2
dc.description.none.fl_txt_mv Electrochemical impedance has been widely used in microbiology in detection, quantifying and identifying microorganisms. Streptomyces strain M7 (SM7) is capable to grow in the presence of organochlorine pesticides (OCPs) as carbon source, using the amino acid asparagine as nitrogen source. This bacterial growth can be valuable and hence used in an impedimetric biosensor to detect and, eventually, quantify these pesticides. Since amino acids frequently used in culture media interfere in impedance determinations, this work presents an optimized design regarding the culture medium capabilities on the impedance response of SM7 activity, as a first stage toward the development of an impedimetric biosensor to detect OCPs. With such a purpose, SM7 growth in liquid or adhered to a solid media was measured by impedance in the presence of asparagine or (NH4)2SO4 as nitrogen source and glucose as a model carbon source. Impedimetric SM7 measurements were performed in the Quantibac instrument that can be employed to evaluate the metabolic behavior of microorganisms in different culture media using two fixed frequencies. We found that SM7 used asparagine as carbon source in the absence of glucose. Since this consumption strongly contributed to the impedance signal, this is not a suitable medium to be used with an impedimetric biosensor. On the other hand, (NH4)2SO4 appears as an ideal medium because it supports SM7 growth in liquid and solid media without interfering the impedimetric detection. This simple and cost-effective technique combined with a spore-based platform is a promising strategy to design OCPs bacteria-based biosensors.
Fil: López Rodriguez, Maria Lorena. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones en Físico-química de Córdoba. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Instituto de Investigaciones en Físico-química de Córdoba; Argentina
Fil: Madrid, Rossana Elena. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto Superior de Investigaciones Biológicas. Universidad Nacional de Tucumán. Instituto Superior de Investigaciones Biológicas; Argentina
Fil: Giacomelli, Carla Eugenia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones en Físico-química de Córdoba. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Instituto de Investigaciones en Físico-química de Córdoba; Argentina
description Electrochemical impedance has been widely used in microbiology in detection, quantifying and identifying microorganisms. Streptomyces strain M7 (SM7) is capable to grow in the presence of organochlorine pesticides (OCPs) as carbon source, using the amino acid asparagine as nitrogen source. This bacterial growth can be valuable and hence used in an impedimetric biosensor to detect and, eventually, quantify these pesticides. Since amino acids frequently used in culture media interfere in impedance determinations, this work presents an optimized design regarding the culture medium capabilities on the impedance response of SM7 activity, as a first stage toward the development of an impedimetric biosensor to detect OCPs. With such a purpose, SM7 growth in liquid or adhered to a solid media was measured by impedance in the presence of asparagine or (NH4)2SO4 as nitrogen source and glucose as a model carbon source. Impedimetric SM7 measurements were performed in the Quantibac instrument that can be employed to evaluate the metabolic behavior of microorganisms in different culture media using two fixed frequencies. We found that SM7 used asparagine as carbon source in the absence of glucose. Since this consumption strongly contributed to the impedance signal, this is not a suitable medium to be used with an impedimetric biosensor. On the other hand, (NH4)2SO4 appears as an ideal medium because it supports SM7 growth in liquid and solid media without interfering the impedimetric detection. This simple and cost-effective technique combined with a spore-based platform is a promising strategy to design OCPs bacteria-based biosensors.
publishDate 2013
dc.date.none.fl_str_mv 2013-12
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/30312
López Rodriguez, Maria Lorena; Madrid, Rossana Elena; Giacomelli, Carla Eugenia; The optimization of the culture medium to design Streptomyces sp. M7 based impedimetric biosensors; Elsevier Science; Sensors and Actuators B: Chemical; 193; 12-2013; 230-237
0925-4005
CONICET Digital
CONICET
url http://hdl.handle.net/11336/30312
identifier_str_mv López Rodriguez, Maria Lorena; Madrid, Rossana Elena; Giacomelli, Carla Eugenia; The optimization of the culture medium to design Streptomyces sp. M7 based impedimetric biosensors; Elsevier Science; Sensors and Actuators B: Chemical; 193; 12-2013; 230-237
0925-4005
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1016/j.snb.2013.11.066
info:eu-repo/semantics/altIdentifier/url/http://www.sciencedirect.com/science/article/pii/S0925400513014160
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Elsevier Science
publisher.none.fl_str_mv Elsevier Science
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.070432

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