Effect of Trehalose and Sucrose on the Hydration and Dipole Potential of Lipid Bilayers

Autores
Luzardo Lorenzo, María del Carmen; Amalfa, F.; Nuñez, A. M.; Díaz, Sonia Beatriz; Biondi de Lopez, A. C.; Disalvo, Edgardo Anibal
Año de publicación
2000
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The water activity in dimyristoylphosphatidylcholine (DMPC) decreases by 60% when the lipid is dehydrated in the presence of trehalose concentrations higher than 0.02 M. In contrast, sucrose in concentrations 10 times higher produced only a 20% decrease in the water activity in the sample. Titrations of a DMPC solution in chloroform yielded 14 water molecules per lipid when pure water was added and seven water molecules per lipid when the titration was done with 0.025 M trehalose. The same concentrations of sucrose produced a turbid solution, which made it impossible to quantify the number of water molecules per lipid. Lipid monolayers spread on an air/water interface showed a decrease from 480 mV in pure water to 425 mV in 0.1 M trehalose. However, the same concentrations of sucrose produced an increase of less than 100 mV. Results obtained with Fourier transform infrared spectroscopy (FTIR) under the same conditions denoted that trehalose binds to the carbonyl groups, while sucrose showed no specific binding. It is concluded that per lipid molecule, 11 of 14 water molecules can be replaced by three trehalose molecules. About four are displaced by changes in the water activity of the bulk solution, and seven by specific interactions with the phospholipids. In this last case, at least two of them are linked to the carbonyls, and this appears to be the cause of the decrease in the dipole potential of the membrane. In contrast, four sucrose molecules displace only three water molecules per lipid, with no effect on the dipole potential or the carbonyl groups.
Fil: Luzardo Lorenzo, María del Carmen. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Química Analítica y Fisicoquímica. Cátedra de Química General e Inorgánica; Argentina
Fil: Amalfa, F.. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Química Analítica y Fisicoquímica. Cátedra de Química General e Inorgánica; Argentina
Fil: Nuñez, A. M.. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Química Analítica y Fisicoquímica. Cátedra de Química General e Inorgánica; Argentina
Fil: Díaz, Sonia Beatriz. Universidad Nacional de Tucumán; Argentina
Fil: Biondi de Lopez, A. C.. Universidad Nacional de Tucumán; Argentina
Fil: Disalvo, Edgardo Anibal. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Química Analítica y Fisicoquímica. Cátedra de Química General e Inorgánica; Argentina
Materia
Interfacial Hydration
Langmuir Monolayers
Liposomes
Ftir
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/71777
Acceder
id CONICETDig_e95c770395253b9015e9882493d9b3a0
oai_identifier_str oai:ri.conicet.gov.ar:11336/71777
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Effect of Trehalose and Sucrose on the Hydration and Dipole Potential of Lipid BilayersLuzardo Lorenzo, María del CarmenAmalfa, F.Nuñez, A. M.Díaz, Sonia BeatrizBiondi de Lopez, A. C.Disalvo, Edgardo AnibalInterfacial HydrationLangmuir MonolayersLiposomesFtirhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1The water activity in dimyristoylphosphatidylcholine (DMPC) decreases by 60% when the lipid is dehydrated in the presence of trehalose concentrations higher than 0.02 M. In contrast, sucrose in concentrations 10 times higher produced only a 20% decrease in the water activity in the sample. Titrations of a DMPC solution in chloroform yielded 14 water molecules per lipid when pure water was added and seven water molecules per lipid when the titration was done with 0.025 M trehalose. The same concentrations of sucrose produced a turbid solution, which made it impossible to quantify the number of water molecules per lipid. Lipid monolayers spread on an air/water interface showed a decrease from 480 mV in pure water to 425 mV in 0.1 M trehalose. However, the same concentrations of sucrose produced an increase of less than 100 mV. Results obtained with Fourier transform infrared spectroscopy (FTIR) under the same conditions denoted that trehalose binds to the carbonyl groups, while sucrose showed no specific binding. It is concluded that per lipid molecule, 11 of 14 water molecules can be replaced by three trehalose molecules. About four are displaced by changes in the water activity of the bulk solution, and seven by specific interactions with the phospholipids. In this last case, at least two of them are linked to the carbonyls, and this appears to be the cause of the decrease in the dipole potential of the membrane. In contrast, four sucrose molecules displace only three water molecules per lipid, with no effect on the dipole potential or the carbonyl groups.Fil: Luzardo Lorenzo, María del Carmen. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Química Analítica y Fisicoquímica. Cátedra de Química General e Inorgánica; ArgentinaFil: Amalfa, F.. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Química Analítica y Fisicoquímica. Cátedra de Química General e Inorgánica; ArgentinaFil: Nuñez, A. M.. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Química Analítica y Fisicoquímica. Cátedra de Química General e Inorgánica; ArgentinaFil: Díaz, Sonia Beatriz. Universidad Nacional de Tucumán; ArgentinaFil: Biondi de Lopez, A. C.. Universidad Nacional de Tucumán; ArgentinaFil: Disalvo, Edgardo Anibal. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Química Analítica y Fisicoquímica. Cátedra de Química General e Inorgánica; ArgentinaCell Press2000-05info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/71777Luzardo Lorenzo, María del Carmen; Amalfa, F.; Nuñez, A. M.; Díaz, Sonia Beatriz; Biondi de Lopez, A. C.; et al.; Effect of Trehalose and Sucrose on the Hydration and Dipole Potential of Lipid Bilayers; Cell Press; Biophysical Journal; 78; 5; 5-2000; 2452-24580006-3495CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1300834/info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S0006349500767890info:eu-repo/semantics/altIdentifier/doi/10.1016/S0006-3495(00)76789-0info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T09:38:32Zoai:ri.conicet.gov.ar:11336/71777instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 09:38:32.613CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Effect of Trehalose and Sucrose on the Hydration and Dipole Potential of Lipid Bilayers
title Effect of Trehalose and Sucrose on the Hydration and Dipole Potential of Lipid Bilayers
spellingShingle Effect of Trehalose and Sucrose on the Hydration and Dipole Potential of Lipid Bilayers
Luzardo Lorenzo, María del Carmen
Interfacial Hydration
Langmuir Monolayers
Liposomes
Ftir
title_short Effect of Trehalose and Sucrose on the Hydration and Dipole Potential of Lipid Bilayers
title_full Effect of Trehalose and Sucrose on the Hydration and Dipole Potential of Lipid Bilayers
title_fullStr Effect of Trehalose and Sucrose on the Hydration and Dipole Potential of Lipid Bilayers
title_full_unstemmed Effect of Trehalose and Sucrose on the Hydration and Dipole Potential of Lipid Bilayers
title_sort Effect of Trehalose and Sucrose on the Hydration and Dipole Potential of Lipid Bilayers
dc.creator.none.fl_str_mv Luzardo Lorenzo, María del Carmen
Amalfa, F.
Nuñez, A. M.
Díaz, Sonia Beatriz
Biondi de Lopez, A. C.
Disalvo, Edgardo Anibal
author Luzardo Lorenzo, María del Carmen
author_facet Luzardo Lorenzo, María del Carmen
Amalfa, F.
Nuñez, A. M.
Díaz, Sonia Beatriz
Biondi de Lopez, A. C.
Disalvo, Edgardo Anibal
author_role author
author2 Amalfa, F.
Nuñez, A. M.
Díaz, Sonia Beatriz
Biondi de Lopez, A. C.
Disalvo, Edgardo Anibal
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv Interfacial Hydration
Langmuir Monolayers
Liposomes
Ftir
topic Interfacial Hydration
Langmuir Monolayers
Liposomes
Ftir
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv The water activity in dimyristoylphosphatidylcholine (DMPC) decreases by 60% when the lipid is dehydrated in the presence of trehalose concentrations higher than 0.02 M. In contrast, sucrose in concentrations 10 times higher produced only a 20% decrease in the water activity in the sample. Titrations of a DMPC solution in chloroform yielded 14 water molecules per lipid when pure water was added and seven water molecules per lipid when the titration was done with 0.025 M trehalose. The same concentrations of sucrose produced a turbid solution, which made it impossible to quantify the number of water molecules per lipid. Lipid monolayers spread on an air/water interface showed a decrease from 480 mV in pure water to 425 mV in 0.1 M trehalose. However, the same concentrations of sucrose produced an increase of less than 100 mV. Results obtained with Fourier transform infrared spectroscopy (FTIR) under the same conditions denoted that trehalose binds to the carbonyl groups, while sucrose showed no specific binding. It is concluded that per lipid molecule, 11 of 14 water molecules can be replaced by three trehalose molecules. About four are displaced by changes in the water activity of the bulk solution, and seven by specific interactions with the phospholipids. In this last case, at least two of them are linked to the carbonyls, and this appears to be the cause of the decrease in the dipole potential of the membrane. In contrast, four sucrose molecules displace only three water molecules per lipid, with no effect on the dipole potential or the carbonyl groups.
Fil: Luzardo Lorenzo, María del Carmen. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Química Analítica y Fisicoquímica. Cátedra de Química General e Inorgánica; Argentina
Fil: Amalfa, F.. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Química Analítica y Fisicoquímica. Cátedra de Química General e Inorgánica; Argentina
Fil: Nuñez, A. M.. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Química Analítica y Fisicoquímica. Cátedra de Química General e Inorgánica; Argentina
Fil: Díaz, Sonia Beatriz. Universidad Nacional de Tucumán; Argentina
Fil: Biondi de Lopez, A. C.. Universidad Nacional de Tucumán; Argentina
Fil: Disalvo, Edgardo Anibal. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Química Analítica y Fisicoquímica. Cátedra de Química General e Inorgánica; Argentina
description The water activity in dimyristoylphosphatidylcholine (DMPC) decreases by 60% when the lipid is dehydrated in the presence of trehalose concentrations higher than 0.02 M. In contrast, sucrose in concentrations 10 times higher produced only a 20% decrease in the water activity in the sample. Titrations of a DMPC solution in chloroform yielded 14 water molecules per lipid when pure water was added and seven water molecules per lipid when the titration was done with 0.025 M trehalose. The same concentrations of sucrose produced a turbid solution, which made it impossible to quantify the number of water molecules per lipid. Lipid monolayers spread on an air/water interface showed a decrease from 480 mV in pure water to 425 mV in 0.1 M trehalose. However, the same concentrations of sucrose produced an increase of less than 100 mV. Results obtained with Fourier transform infrared spectroscopy (FTIR) under the same conditions denoted that trehalose binds to the carbonyl groups, while sucrose showed no specific binding. It is concluded that per lipid molecule, 11 of 14 water molecules can be replaced by three trehalose molecules. About four are displaced by changes in the water activity of the bulk solution, and seven by specific interactions with the phospholipids. In this last case, at least two of them are linked to the carbonyls, and this appears to be the cause of the decrease in the dipole potential of the membrane. In contrast, four sucrose molecules displace only three water molecules per lipid, with no effect on the dipole potential or the carbonyl groups.
publishDate 2000
dc.date.none.fl_str_mv 2000-05
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/71777
Luzardo Lorenzo, María del Carmen; Amalfa, F.; Nuñez, A. M.; Díaz, Sonia Beatriz; Biondi de Lopez, A. C.; et al.; Effect of Trehalose and Sucrose on the Hydration and Dipole Potential of Lipid Bilayers; Cell Press; Biophysical Journal; 78; 5; 5-2000; 2452-2458
0006-3495
CONICET Digital
CONICET
url http://hdl.handle.net/11336/71777
identifier_str_mv Luzardo Lorenzo, María del Carmen; Amalfa, F.; Nuñez, A. M.; Díaz, Sonia Beatriz; Biondi de Lopez, A. C.; et al.; Effect of Trehalose and Sucrose on the Hydration and Dipole Potential of Lipid Bilayers; Cell Press; Biophysical Journal; 78; 5; 5-2000; 2452-2458
0006-3495
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1300834/
info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S0006349500767890
info:eu-repo/semantics/altIdentifier/doi/10.1016/S0006-3495(00)76789-0
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Cell Press
publisher.none.fl_str_mv Cell Press
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.070432

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