Reorganization of Hydration Water of DPPC Multilamellar Vesicles Induced by l -Cysteine Interaction

Autores
Arias, Juan Marcelo; Tuttolomondo, María Eugenia; Díaz, Sonia Beatriz; Ben Altabef, Aída
Año de publicación
2018
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The aim of this study is to analyze the consequences of water redistribution on the structure and stability of phospholipid bilayers induced by cysteine (Cys). This interaction is studied with 1,2-dipalmitoyl-sn-glycero-3-phosphatidylcholine (DPPC) multilamellar vesicles in gel (30 °C) and liquid crystalline (50 °C) state; experimental studies were performed by means of Fourier transform infrared (FTIR) spectroscopy, Raman spectroscopy, and differential scanning calorimetry (DSC). The polar head sites of the lipid molecules to which water can bind are identified by competition with compounds that form hydrogen bonds, such as Cys. FTIR spectroscopy results revealed that there is a Cys interaction with the phospholipid head groups in the gel and liquid crystalline phases. Raman spectra were measured in the gel state. They were dominated by vibrations of the fatty acyl chains, with superposition of a few bands from the head group, and clearly showed that the S-H stretching band of Cys shifted to lower frequencies with a decrease in its force constant. DSC disclosed an overview of the behavior of the multilamellar vesicles in the working temperature range (30-50 °C) and showed how the increase of the molar ratios modified the environment of the polar head and the hydrocarbon chains. A loss of the pretransition (TP) and an increase in the temperature of main transition (Tm) with increasing Cys/DPPC molar ratio were observed.
Fil: Arias, Juan Marcelo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto de Química del Noroeste. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia. Instituto de Química del Noroeste; Argentina
Fil: Tuttolomondo, María Eugenia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto de Química del Noroeste. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia. Instituto de Química del Noroeste; Argentina
Fil: Díaz, Sonia Beatriz. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto de Química del Noroeste. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia. Instituto de Química del Noroeste; Argentina
Fil: Ben Altabef, Aída. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto de Química del Noroeste. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia. Instituto de Química del Noroeste; Argentina
Materia
LIPOSOME
INTERACTION
HYDRATION
FTIR
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/90997
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/90997
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Reorganization of Hydration Water of DPPC Multilamellar Vesicles Induced by l -Cysteine InteractionArias, Juan MarceloTuttolomondo, María EugeniaDíaz, Sonia BeatrizBen Altabef, AídaLIPOSOMEINTERACTIONHYDRATIONFTIRhttps://purl.org/becyt/ford/1.4https://purl.org/becyt/ford/1The aim of this study is to analyze the consequences of water redistribution on the structure and stability of phospholipid bilayers induced by cysteine (Cys). This interaction is studied with 1,2-dipalmitoyl-sn-glycero-3-phosphatidylcholine (DPPC) multilamellar vesicles in gel (30 °C) and liquid crystalline (50 °C) state; experimental studies were performed by means of Fourier transform infrared (FTIR) spectroscopy, Raman spectroscopy, and differential scanning calorimetry (DSC). The polar head sites of the lipid molecules to which water can bind are identified by competition with compounds that form hydrogen bonds, such as Cys. FTIR spectroscopy results revealed that there is a Cys interaction with the phospholipid head groups in the gel and liquid crystalline phases. Raman spectra were measured in the gel state. They were dominated by vibrations of the fatty acyl chains, with superposition of a few bands from the head group, and clearly showed that the S-H stretching band of Cys shifted to lower frequencies with a decrease in its force constant. DSC disclosed an overview of the behavior of the multilamellar vesicles in the working temperature range (30-50 °C) and showed how the increase of the molar ratios modified the environment of the polar head and the hydrocarbon chains. A loss of the pretransition (TP) and an increase in the temperature of main transition (Tm) with increasing Cys/DPPC molar ratio were observed.Fil: Arias, Juan Marcelo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto de Química del Noroeste. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia. Instituto de Química del Noroeste; ArgentinaFil: Tuttolomondo, María Eugenia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto de Química del Noroeste. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia. Instituto de Química del Noroeste; ArgentinaFil: Díaz, Sonia Beatriz. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto de Química del Noroeste. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia. Instituto de Química del Noroeste; ArgentinaFil: Ben Altabef, Aída. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto de Química del Noroeste. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia. Instituto de Química del Noroeste; ArgentinaAmerican Chemical Society2018-05info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/90997Arias, Juan Marcelo; Tuttolomondo, María Eugenia; Díaz, Sonia Beatriz; Ben Altabef, Aída; Reorganization of Hydration Water of DPPC Multilamellar Vesicles Induced by l -Cysteine Interaction; American Chemical Society; Journal of Physical Chemistry B; 122; 20; 5-2018; 5193-52041520-6106CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/http://pubs.acs.org/doi/10.1021/acs.jpcb.8b01721info:eu-repo/semantics/altIdentifier/doi/10.1021/acs.jpcb.8b01721info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-10T13:02:27Zoai:ri.conicet.gov.ar:11336/90997instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-10 13:02:27.785CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Reorganization of Hydration Water of DPPC Multilamellar Vesicles Induced by l -Cysteine Interaction
title Reorganization of Hydration Water of DPPC Multilamellar Vesicles Induced by l -Cysteine Interaction
spellingShingle Reorganization of Hydration Water of DPPC Multilamellar Vesicles Induced by l -Cysteine Interaction
Arias, Juan Marcelo
LIPOSOME
INTERACTION
HYDRATION
FTIR
title_short Reorganization of Hydration Water of DPPC Multilamellar Vesicles Induced by l -Cysteine Interaction
title_full Reorganization of Hydration Water of DPPC Multilamellar Vesicles Induced by l -Cysteine Interaction
title_fullStr Reorganization of Hydration Water of DPPC Multilamellar Vesicles Induced by l -Cysteine Interaction
title_full_unstemmed Reorganization of Hydration Water of DPPC Multilamellar Vesicles Induced by l -Cysteine Interaction
title_sort Reorganization of Hydration Water of DPPC Multilamellar Vesicles Induced by l -Cysteine Interaction
dc.creator.none.fl_str_mv Arias, Juan Marcelo
Tuttolomondo, María Eugenia
Díaz, Sonia Beatriz
Ben Altabef, Aída
author Arias, Juan Marcelo
author_facet Arias, Juan Marcelo
Tuttolomondo, María Eugenia
Díaz, Sonia Beatriz
Ben Altabef, Aída
author_role author
author2 Tuttolomondo, María Eugenia
Díaz, Sonia Beatriz
Ben Altabef, Aída
author2_role author
author
author
dc.subject.none.fl_str_mv LIPOSOME
INTERACTION
HYDRATION
FTIR
topic LIPOSOME
INTERACTION
HYDRATION
FTIR
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.4
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv The aim of this study is to analyze the consequences of water redistribution on the structure and stability of phospholipid bilayers induced by cysteine (Cys). This interaction is studied with 1,2-dipalmitoyl-sn-glycero-3-phosphatidylcholine (DPPC) multilamellar vesicles in gel (30 °C) and liquid crystalline (50 °C) state; experimental studies were performed by means of Fourier transform infrared (FTIR) spectroscopy, Raman spectroscopy, and differential scanning calorimetry (DSC). The polar head sites of the lipid molecules to which water can bind are identified by competition with compounds that form hydrogen bonds, such as Cys. FTIR spectroscopy results revealed that there is a Cys interaction with the phospholipid head groups in the gel and liquid crystalline phases. Raman spectra were measured in the gel state. They were dominated by vibrations of the fatty acyl chains, with superposition of a few bands from the head group, and clearly showed that the S-H stretching band of Cys shifted to lower frequencies with a decrease in its force constant. DSC disclosed an overview of the behavior of the multilamellar vesicles in the working temperature range (30-50 °C) and showed how the increase of the molar ratios modified the environment of the polar head and the hydrocarbon chains. A loss of the pretransition (TP) and an increase in the temperature of main transition (Tm) with increasing Cys/DPPC molar ratio were observed.
Fil: Arias, Juan Marcelo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto de Química del Noroeste. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia. Instituto de Química del Noroeste; Argentina
Fil: Tuttolomondo, María Eugenia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto de Química del Noroeste. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia. Instituto de Química del Noroeste; Argentina
Fil: Díaz, Sonia Beatriz. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto de Química del Noroeste. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia. Instituto de Química del Noroeste; Argentina
Fil: Ben Altabef, Aída. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto de Química del Noroeste. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia. Instituto de Química del Noroeste; Argentina
description The aim of this study is to analyze the consequences of water redistribution on the structure and stability of phospholipid bilayers induced by cysteine (Cys). This interaction is studied with 1,2-dipalmitoyl-sn-glycero-3-phosphatidylcholine (DPPC) multilamellar vesicles in gel (30 °C) and liquid crystalline (50 °C) state; experimental studies were performed by means of Fourier transform infrared (FTIR) spectroscopy, Raman spectroscopy, and differential scanning calorimetry (DSC). The polar head sites of the lipid molecules to which water can bind are identified by competition with compounds that form hydrogen bonds, such as Cys. FTIR spectroscopy results revealed that there is a Cys interaction with the phospholipid head groups in the gel and liquid crystalline phases. Raman spectra were measured in the gel state. They were dominated by vibrations of the fatty acyl chains, with superposition of a few bands from the head group, and clearly showed that the S-H stretching band of Cys shifted to lower frequencies with a decrease in its force constant. DSC disclosed an overview of the behavior of the multilamellar vesicles in the working temperature range (30-50 °C) and showed how the increase of the molar ratios modified the environment of the polar head and the hydrocarbon chains. A loss of the pretransition (TP) and an increase in the temperature of main transition (Tm) with increasing Cys/DPPC molar ratio were observed.
publishDate 2018
dc.date.none.fl_str_mv 2018-05
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/90997
Arias, Juan Marcelo; Tuttolomondo, María Eugenia; Díaz, Sonia Beatriz; Ben Altabef, Aída; Reorganization of Hydration Water of DPPC Multilamellar Vesicles Induced by l -Cysteine Interaction; American Chemical Society; Journal of Physical Chemistry B; 122; 20; 5-2018; 5193-5204
1520-6106
CONICET Digital
CONICET
url http://hdl.handle.net/11336/90997
identifier_str_mv Arias, Juan Marcelo; Tuttolomondo, María Eugenia; Díaz, Sonia Beatriz; Ben Altabef, Aída; Reorganization of Hydration Water of DPPC Multilamellar Vesicles Induced by l -Cysteine Interaction; American Chemical Society; Journal of Physical Chemistry B; 122; 20; 5-2018; 5193-5204
1520-6106
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/http://pubs.acs.org/doi/10.1021/acs.jpcb.8b01721
info:eu-repo/semantics/altIdentifier/doi/10.1021/acs.jpcb.8b01721
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv American Chemical Society
publisher.none.fl_str_mv American Chemical Society
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 12.993085

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