Direct analysis of nectar and floral volatile organic compounds in hybrid onions by HS-SPME/GC-MS: Relationship with pollination and seed production

Autores
Soto Vargas, Verónica Carolina; Maldonado, Irma B.; Jofre, Viviana Patricia; Galmarini, Claudio Romulo; Silva, María Fernanda
Año de publicación
2015
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
In this study, a simple and solvent-free method using headspace solid-phase microextraction (SPME) and gas chromatography-mass spectrometry (GC-MS) was developed for determination of the volatile compounds from fresh flowers and nectar of male sterile and fertile lines of Allium cepa L. The SPME parameters were studied; the optimum conditions of 85. μm carboxen/polydimethylsiloxane (CAR/PDMS), extraction temperature of 30. °C and extraction time of 30. min were obtained. The analytical method was applied to characterize different onion lines according to the chemical composition of the volatile fraction of nectar and flowers. On the other hand, it was determined which odor components contribute to the pollination of onions lines and relate the content of specific analytes with foraging behavior and seed production. More than 90 compounds were identified. The samples studied showed differences in the volatile profiles of flowers and nectar among the different lines. The results demonstrated that headspace SPME-GCMS is a simple, rapid and solvent-free method suitable for analysis of volatile compounds emitted from onion plants. Furthermore, a great difference was found for the number of bee visits and seed yield among the onion lines analyzed. This study demonstrates that the combination of chemical information and statistical analysis is able to differentiate onion male sterile lines from fertile lines, as well as among male sterile lines. Repellent compounds, such as dioxolanes, piperidines and organosulfurs may contribute negatively to pollination, since the higher content of these analytes, the less bee visits and seed production yield.
Fil: Soto Vargas, Verónica Carolina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Biología Agrícola de Mendoza. Universidad Nacional de Cuyo. Facultad de Ciencias Agrarias. Instituto de Biología Agrícola de Mendoza; Argentina
Fil: Maldonado, Irma B.. Instituto Nacional de Tecnología Agropecuaria; Argentina
Fil: Jofre, Viviana Patricia. Instituto Nacional de Tecnología Agropecuaria; Argentina
Fil: Galmarini, Claudio Romulo. Instituto Nacional de Tecnología Agropecuaria; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza; Argentina. Universidad Nacional de Cuyo. Facultad de Ciencias Agrarias; Argentina
Fil: Silva, María Fernanda. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Biología Agrícola de Mendoza. Universidad Nacional de Cuyo. Facultad de Ciencias Agrarias. Instituto de Biología Agrícola de Mendoza; Argentina
Materia
Allium Cepa L
Gas Chromatography-Mass Spectrometry (Gc-Ms)
Male-Sterility
Solid-Phase Microextraction (Spme)
Volatile Organic Compounds (Vocs)
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/40046
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/40046
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Direct analysis of nectar and floral volatile organic compounds in hybrid onions by HS-SPME/GC-MS: Relationship with pollination and seed productionSoto Vargas, Verónica CarolinaMaldonado, Irma B.Jofre, Viviana PatriciaGalmarini, Claudio RomuloSilva, María FernandaAllium Cepa LGas Chromatography-Mass Spectrometry (Gc-Ms)Male-SterilitySolid-Phase Microextraction (Spme)Volatile Organic Compounds (Vocs)https://purl.org/becyt/ford/4.1https://purl.org/becyt/ford/4In this study, a simple and solvent-free method using headspace solid-phase microextraction (SPME) and gas chromatography-mass spectrometry (GC-MS) was developed for determination of the volatile compounds from fresh flowers and nectar of male sterile and fertile lines of Allium cepa L. The SPME parameters were studied; the optimum conditions of 85. μm carboxen/polydimethylsiloxane (CAR/PDMS), extraction temperature of 30. °C and extraction time of 30. min were obtained. The analytical method was applied to characterize different onion lines according to the chemical composition of the volatile fraction of nectar and flowers. On the other hand, it was determined which odor components contribute to the pollination of onions lines and relate the content of specific analytes with foraging behavior and seed production. More than 90 compounds were identified. The samples studied showed differences in the volatile profiles of flowers and nectar among the different lines. The results demonstrated that headspace SPME-GCMS is a simple, rapid and solvent-free method suitable for analysis of volatile compounds emitted from onion plants. Furthermore, a great difference was found for the number of bee visits and seed yield among the onion lines analyzed. This study demonstrates that the combination of chemical information and statistical analysis is able to differentiate onion male sterile lines from fertile lines, as well as among male sterile lines. Repellent compounds, such as dioxolanes, piperidines and organosulfurs may contribute negatively to pollination, since the higher content of these analytes, the less bee visits and seed production yield.Fil: Soto Vargas, Verónica Carolina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Biología Agrícola de Mendoza. Universidad Nacional de Cuyo. Facultad de Ciencias Agrarias. Instituto de Biología Agrícola de Mendoza; ArgentinaFil: Maldonado, Irma B.. Instituto Nacional de Tecnología Agropecuaria; ArgentinaFil: Jofre, Viviana Patricia. Instituto Nacional de Tecnología Agropecuaria; ArgentinaFil: Galmarini, Claudio Romulo. Instituto Nacional de Tecnología Agropecuaria; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza; Argentina. Universidad Nacional de Cuyo. Facultad de Ciencias Agrarias; ArgentinaFil: Silva, María Fernanda. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Biología Agrícola de Mendoza. Universidad Nacional de Cuyo. Facultad de Ciencias Agrarias. Instituto de Biología Agrícola de Mendoza; ArgentinaElsevier Science2015-09info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/40046Soto Vargas, Verónica Carolina; Maldonado, Irma B.; Jofre, Viviana Patricia; Galmarini, Claudio Romulo; Silva, María Fernanda; Direct analysis of nectar and floral volatile organic compounds in hybrid onions by HS-SPME/GC-MS: Relationship with pollination and seed production; Elsevier Science; Microchemical Journal; 122; 9-2015; 110-1180026-265XCONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S0026265X15000788info:eu-repo/semantics/altIdentifier/doi/10.1016/j.microc.2015.04.017info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-15T15:28:56Zoai:ri.conicet.gov.ar:11336/40046instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-15 15:28:57.242CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Direct analysis of nectar and floral volatile organic compounds in hybrid onions by HS-SPME/GC-MS: Relationship with pollination and seed production
title Direct analysis of nectar and floral volatile organic compounds in hybrid onions by HS-SPME/GC-MS: Relationship with pollination and seed production
spellingShingle Direct analysis of nectar and floral volatile organic compounds in hybrid onions by HS-SPME/GC-MS: Relationship with pollination and seed production
Soto Vargas, Verónica Carolina
Allium Cepa L
Gas Chromatography-Mass Spectrometry (Gc-Ms)
Male-Sterility
Solid-Phase Microextraction (Spme)
Volatile Organic Compounds (Vocs)
title_short Direct analysis of nectar and floral volatile organic compounds in hybrid onions by HS-SPME/GC-MS: Relationship with pollination and seed production
title_full Direct analysis of nectar and floral volatile organic compounds in hybrid onions by HS-SPME/GC-MS: Relationship with pollination and seed production
title_fullStr Direct analysis of nectar and floral volatile organic compounds in hybrid onions by HS-SPME/GC-MS: Relationship with pollination and seed production
title_full_unstemmed Direct analysis of nectar and floral volatile organic compounds in hybrid onions by HS-SPME/GC-MS: Relationship with pollination and seed production
title_sort Direct analysis of nectar and floral volatile organic compounds in hybrid onions by HS-SPME/GC-MS: Relationship with pollination and seed production
dc.creator.none.fl_str_mv Soto Vargas, Verónica Carolina
Maldonado, Irma B.
Jofre, Viviana Patricia
Galmarini, Claudio Romulo
Silva, María Fernanda
author Soto Vargas, Verónica Carolina
author_facet Soto Vargas, Verónica Carolina
Maldonado, Irma B.
Jofre, Viviana Patricia
Galmarini, Claudio Romulo
Silva, María Fernanda
author_role author
author2 Maldonado, Irma B.
Jofre, Viviana Patricia
Galmarini, Claudio Romulo
Silva, María Fernanda
author2_role author
author
author
author
dc.subject.none.fl_str_mv Allium Cepa L
Gas Chromatography-Mass Spectrometry (Gc-Ms)
Male-Sterility
Solid-Phase Microextraction (Spme)
Volatile Organic Compounds (Vocs)
topic Allium Cepa L
Gas Chromatography-Mass Spectrometry (Gc-Ms)
Male-Sterility
Solid-Phase Microextraction (Spme)
Volatile Organic Compounds (Vocs)
purl_subject.fl_str_mv https://purl.org/becyt/ford/4.1
https://purl.org/becyt/ford/4
dc.description.none.fl_txt_mv In this study, a simple and solvent-free method using headspace solid-phase microextraction (SPME) and gas chromatography-mass spectrometry (GC-MS) was developed for determination of the volatile compounds from fresh flowers and nectar of male sterile and fertile lines of Allium cepa L. The SPME parameters were studied; the optimum conditions of 85. μm carboxen/polydimethylsiloxane (CAR/PDMS), extraction temperature of 30. °C and extraction time of 30. min were obtained. The analytical method was applied to characterize different onion lines according to the chemical composition of the volatile fraction of nectar and flowers. On the other hand, it was determined which odor components contribute to the pollination of onions lines and relate the content of specific analytes with foraging behavior and seed production. More than 90 compounds were identified. The samples studied showed differences in the volatile profiles of flowers and nectar among the different lines. The results demonstrated that headspace SPME-GCMS is a simple, rapid and solvent-free method suitable for analysis of volatile compounds emitted from onion plants. Furthermore, a great difference was found for the number of bee visits and seed yield among the onion lines analyzed. This study demonstrates that the combination of chemical information and statistical analysis is able to differentiate onion male sterile lines from fertile lines, as well as among male sterile lines. Repellent compounds, such as dioxolanes, piperidines and organosulfurs may contribute negatively to pollination, since the higher content of these analytes, the less bee visits and seed production yield.
Fil: Soto Vargas, Verónica Carolina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Biología Agrícola de Mendoza. Universidad Nacional de Cuyo. Facultad de Ciencias Agrarias. Instituto de Biología Agrícola de Mendoza; Argentina
Fil: Maldonado, Irma B.. Instituto Nacional de Tecnología Agropecuaria; Argentina
Fil: Jofre, Viviana Patricia. Instituto Nacional de Tecnología Agropecuaria; Argentina
Fil: Galmarini, Claudio Romulo. Instituto Nacional de Tecnología Agropecuaria; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza; Argentina. Universidad Nacional de Cuyo. Facultad de Ciencias Agrarias; Argentina
Fil: Silva, María Fernanda. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Biología Agrícola de Mendoza. Universidad Nacional de Cuyo. Facultad de Ciencias Agrarias. Instituto de Biología Agrícola de Mendoza; Argentina
description In this study, a simple and solvent-free method using headspace solid-phase microextraction (SPME) and gas chromatography-mass spectrometry (GC-MS) was developed for determination of the volatile compounds from fresh flowers and nectar of male sterile and fertile lines of Allium cepa L. The SPME parameters were studied; the optimum conditions of 85. μm carboxen/polydimethylsiloxane (CAR/PDMS), extraction temperature of 30. °C and extraction time of 30. min were obtained. The analytical method was applied to characterize different onion lines according to the chemical composition of the volatile fraction of nectar and flowers. On the other hand, it was determined which odor components contribute to the pollination of onions lines and relate the content of specific analytes with foraging behavior and seed production. More than 90 compounds were identified. The samples studied showed differences in the volatile profiles of flowers and nectar among the different lines. The results demonstrated that headspace SPME-GCMS is a simple, rapid and solvent-free method suitable for analysis of volatile compounds emitted from onion plants. Furthermore, a great difference was found for the number of bee visits and seed yield among the onion lines analyzed. This study demonstrates that the combination of chemical information and statistical analysis is able to differentiate onion male sterile lines from fertile lines, as well as among male sterile lines. Repellent compounds, such as dioxolanes, piperidines and organosulfurs may contribute negatively to pollination, since the higher content of these analytes, the less bee visits and seed production yield.
publishDate 2015
dc.date.none.fl_str_mv 2015-09
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/40046
Soto Vargas, Verónica Carolina; Maldonado, Irma B.; Jofre, Viviana Patricia; Galmarini, Claudio Romulo; Silva, María Fernanda; Direct analysis of nectar and floral volatile organic compounds in hybrid onions by HS-SPME/GC-MS: Relationship with pollination and seed production; Elsevier Science; Microchemical Journal; 122; 9-2015; 110-118
0026-265X
CONICET Digital
CONICET
url http://hdl.handle.net/11336/40046
identifier_str_mv Soto Vargas, Verónica Carolina; Maldonado, Irma B.; Jofre, Viviana Patricia; Galmarini, Claudio Romulo; Silva, María Fernanda; Direct analysis of nectar and floral volatile organic compounds in hybrid onions by HS-SPME/GC-MS: Relationship with pollination and seed production; Elsevier Science; Microchemical Journal; 122; 9-2015; 110-118
0026-265X
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S0026265X15000788
info:eu-repo/semantics/altIdentifier/doi/10.1016/j.microc.2015.04.017
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Elsevier Science
publisher.none.fl_str_mv Elsevier Science
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.22299

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