Direct analysis of néctar and floral volatile organic compounds in hybrid onions by HS-SPME/GC-MS: Relationship with pollination and seed production

Autores
Soto Vargas, Verónica Carolina; Maldonado, Irma Beatriz; Jofre, Viviana Patricia; Galmarini, Claudio Romulo; Silva, Maria Fernanda
Año de publicación
2016
Idioma
inglés
Tipo de recurso
artículo
Estado
versión aceptada
Descripción
In this study, a simple and solvent-free method using headspace solid-phase microextraction (SPME) and gas chromatography–mass spectrometry (GC–MS) was developed for determination of the volatile compounds from fresh flowers and nectar of male sterile and fertile lines of Allium cepa L. The SPME parameters were studied; the optimum conditions of 85 μm carboxen/polydimethylsiloxane (CAR/PDMS), extraction temperature of 30 °C and extraction time of 30 min were obtained. The analytical method was applied to characterize different onion lines according to the chemical composition of the volatile fraction of nectar and flowers. On the other hand, it was determined which odor components contribute to the pollination of onions lines and relate the content of specific analytes with foraging behavior and seed production. More than 90 compounds were identified. The samples studied showed differences in the volatile profiles of flowers and nectar among the different lines. The results demonstrated that headspace SPME–GCMS is a simple, rapid and solvent-free method suitable for analysis of volatile compounds emitted from onion plants. Furthermore, a great difference was found for the number of bee visits and seed yield among the onion lines analyzed. This study demonstrates that the combination of chemical information and statistical analysis is able to differentiate onion male sterile lines from fertile lines, as well as among male sterile lines. Repellent compounds, such as dioxolanes, piperidines and organosulfurs may contribute negatively to pollination, since the higher content of these analytes, the less bee visits and seed production yield.
EEA Mendoza
Fil: Soto, Veronica Carolina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Biología Agrícola de Mendoza. Universidad Nacional de Cuyo. Facultad de Ciencias Agrarias. Instituto de Biología Agrícola de Mendoza; Argentina
Fil: Maldonado, Irma Beatriz. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria El Colorado. Agencia de Extensión Rural Formosa; Argentina
Fil: Jofre, Viviana Patricia. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Mendoza; Argentina
Fil: Galmarini, Claudio Romulo. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria La Consulta; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de Cuyo. Facultad de Ciencia Agrarias: Argentina
Fil: Silva, Maria Fernanda. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Biología Agrícola de Mendoza. Universidad Nacional de Cuyo. Facultad de Ciencias Agrarias. Instituto de Biología Agrícola de Mendoza; Argentina
Fuente
Microchemical journal 122 : 110-118. (September 2015)
Materia
Cebolla
Allium Cepa
Néctar
Compuestos Ogánicos Volátiles
Polinización
Producción de Semillas
Onions
Oganic Vlatile Compounds
Pollination
Seed Production
Gas Chromatography
SPME
GC–MS
Nivel de accesibilidad
acceso restringido
Condiciones de uso
Repositorio
INTA Digital (INTA)
Institución
Instituto Nacional de Tecnología Agropecuaria
OAI Identificador
oai:localhost:20.500.12123/1375
Acceder
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oai_identifier_str oai:localhost:20.500.12123/1375
network_acronym_str INTADig
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network_name_str INTA Digital (INTA)
spelling Direct analysis of néctar and floral volatile organic compounds in hybrid onions by HS-SPME/GC-MS: Relationship with pollination and seed productionSoto Vargas, Verónica CarolinaMaldonado, Irma BeatrizJofre, Viviana PatriciaGalmarini, Claudio RomuloSilva, Maria FernandaCebollaAllium CepaNéctarCompuestos Ogánicos VolátilesPolinizaciónProducción de SemillasOnionsOganic Vlatile CompoundsPollinationSeed ProductionGas ChromatographySPMEGC–MSIn this study, a simple and solvent-free method using headspace solid-phase microextraction (SPME) and gas chromatography–mass spectrometry (GC–MS) was developed for determination of the volatile compounds from fresh flowers and nectar of male sterile and fertile lines of Allium cepa L. The SPME parameters were studied; the optimum conditions of 85 μm carboxen/polydimethylsiloxane (CAR/PDMS), extraction temperature of 30 °C and extraction time of 30 min were obtained. The analytical method was applied to characterize different onion lines according to the chemical composition of the volatile fraction of nectar and flowers. On the other hand, it was determined which odor components contribute to the pollination of onions lines and relate the content of specific analytes with foraging behavior and seed production. More than 90 compounds were identified. The samples studied showed differences in the volatile profiles of flowers and nectar among the different lines. The results demonstrated that headspace SPME–GCMS is a simple, rapid and solvent-free method suitable for analysis of volatile compounds emitted from onion plants. Furthermore, a great difference was found for the number of bee visits and seed yield among the onion lines analyzed. This study demonstrates that the combination of chemical information and statistical analysis is able to differentiate onion male sterile lines from fertile lines, as well as among male sterile lines. Repellent compounds, such as dioxolanes, piperidines and organosulfurs may contribute negatively to pollination, since the higher content of these analytes, the less bee visits and seed production yield.EEA MendozaFil: Soto, Veronica Carolina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Biología Agrícola de Mendoza. Universidad Nacional de Cuyo. Facultad de Ciencias Agrarias. Instituto de Biología Agrícola de Mendoza; ArgentinaFil: Maldonado, Irma Beatriz. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria El Colorado. Agencia de Extensión Rural Formosa; ArgentinaFil: Jofre, Viviana Patricia. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Mendoza; ArgentinaFil: Galmarini, Claudio Romulo. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria La Consulta; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de Cuyo. Facultad de Ciencia Agrarias: ArgentinaFil: Silva, Maria Fernanda. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Biología Agrícola de Mendoza. Universidad Nacional de Cuyo. Facultad de Ciencias Agrarias. Instituto de Biología Agrícola de Mendoza; Argentina2017-10-02T14:16:06Z2017-10-02T14:16:06Z2016info:eu-repo/semantics/articleinfo:eu-repo/semantics/acceptedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttp://hdl.handle.net/20.500.12123/1375http://www.sciencedirect.com/science/article/pii/S0026265X15000788https://doi.org/10.1016/j.microc.2015.04.017Microchemical journal 122 : 110-118. (September 2015)reponame:INTA Digital (INTA)instname:Instituto Nacional de Tecnología Agropecuariaenginfo:eu-repo/semantics/restrictedAccess2025-09-04T09:47:04Zoai:localhost:20.500.12123/1375instacron:INTAInstitucionalhttp://repositorio.inta.gob.ar/Organismo científico-tecnológicoNo correspondehttp://repositorio.inta.gob.ar/oai/requesttripaldi.nicolas@inta.gob.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:l2025-09-04 09:47:05.487INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuariafalse
dc.title.none.fl_str_mv Direct analysis of néctar and floral volatile organic compounds in hybrid onions by HS-SPME/GC-MS: Relationship with pollination and seed production
title Direct analysis of néctar and floral volatile organic compounds in hybrid onions by HS-SPME/GC-MS: Relationship with pollination and seed production
spellingShingle Direct analysis of néctar and floral volatile organic compounds in hybrid onions by HS-SPME/GC-MS: Relationship with pollination and seed production
Soto Vargas, Verónica Carolina
Cebolla
Allium Cepa
Néctar
Compuestos Ogánicos Volátiles
Polinización
Producción de Semillas
Onions
Oganic Vlatile Compounds
Pollination
Seed Production
Gas Chromatography
SPME
GC–MS
title_short Direct analysis of néctar and floral volatile organic compounds in hybrid onions by HS-SPME/GC-MS: Relationship with pollination and seed production
title_full Direct analysis of néctar and floral volatile organic compounds in hybrid onions by HS-SPME/GC-MS: Relationship with pollination and seed production
title_fullStr Direct analysis of néctar and floral volatile organic compounds in hybrid onions by HS-SPME/GC-MS: Relationship with pollination and seed production
title_full_unstemmed Direct analysis of néctar and floral volatile organic compounds in hybrid onions by HS-SPME/GC-MS: Relationship with pollination and seed production
title_sort Direct analysis of néctar and floral volatile organic compounds in hybrid onions by HS-SPME/GC-MS: Relationship with pollination and seed production
dc.creator.none.fl_str_mv Soto Vargas, Verónica Carolina
Maldonado, Irma Beatriz
Jofre, Viviana Patricia
Galmarini, Claudio Romulo
Silva, Maria Fernanda
author Soto Vargas, Verónica Carolina
author_facet Soto Vargas, Verónica Carolina
Maldonado, Irma Beatriz
Jofre, Viviana Patricia
Galmarini, Claudio Romulo
Silva, Maria Fernanda
author_role author
author2 Maldonado, Irma Beatriz
Jofre, Viviana Patricia
Galmarini, Claudio Romulo
Silva, Maria Fernanda
author2_role author
author
author
author
dc.subject.none.fl_str_mv Cebolla
Allium Cepa
Néctar
Compuestos Ogánicos Volátiles
Polinización
Producción de Semillas
Onions
Oganic Vlatile Compounds
Pollination
Seed Production
Gas Chromatography
SPME
GC–MS
topic Cebolla
Allium Cepa
Néctar
Compuestos Ogánicos Volátiles
Polinización
Producción de Semillas
Onions
Oganic Vlatile Compounds
Pollination
Seed Production
Gas Chromatography
SPME
GC–MS
dc.description.none.fl_txt_mv In this study, a simple and solvent-free method using headspace solid-phase microextraction (SPME) and gas chromatography–mass spectrometry (GC–MS) was developed for determination of the volatile compounds from fresh flowers and nectar of male sterile and fertile lines of Allium cepa L. The SPME parameters were studied; the optimum conditions of 85 μm carboxen/polydimethylsiloxane (CAR/PDMS), extraction temperature of 30 °C and extraction time of 30 min were obtained. The analytical method was applied to characterize different onion lines according to the chemical composition of the volatile fraction of nectar and flowers. On the other hand, it was determined which odor components contribute to the pollination of onions lines and relate the content of specific analytes with foraging behavior and seed production. More than 90 compounds were identified. The samples studied showed differences in the volatile profiles of flowers and nectar among the different lines. The results demonstrated that headspace SPME–GCMS is a simple, rapid and solvent-free method suitable for analysis of volatile compounds emitted from onion plants. Furthermore, a great difference was found for the number of bee visits and seed yield among the onion lines analyzed. This study demonstrates that the combination of chemical information and statistical analysis is able to differentiate onion male sterile lines from fertile lines, as well as among male sterile lines. Repellent compounds, such as dioxolanes, piperidines and organosulfurs may contribute negatively to pollination, since the higher content of these analytes, the less bee visits and seed production yield.
EEA Mendoza
Fil: Soto, Veronica Carolina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Biología Agrícola de Mendoza. Universidad Nacional de Cuyo. Facultad de Ciencias Agrarias. Instituto de Biología Agrícola de Mendoza; Argentina
Fil: Maldonado, Irma Beatriz. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria El Colorado. Agencia de Extensión Rural Formosa; Argentina
Fil: Jofre, Viviana Patricia. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Mendoza; Argentina
Fil: Galmarini, Claudio Romulo. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria La Consulta; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de Cuyo. Facultad de Ciencia Agrarias: Argentina
Fil: Silva, Maria Fernanda. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Biología Agrícola de Mendoza. Universidad Nacional de Cuyo. Facultad de Ciencias Agrarias. Instituto de Biología Agrícola de Mendoza; Argentina
description In this study, a simple and solvent-free method using headspace solid-phase microextraction (SPME) and gas chromatography–mass spectrometry (GC–MS) was developed for determination of the volatile compounds from fresh flowers and nectar of male sterile and fertile lines of Allium cepa L. The SPME parameters were studied; the optimum conditions of 85 μm carboxen/polydimethylsiloxane (CAR/PDMS), extraction temperature of 30 °C and extraction time of 30 min were obtained. The analytical method was applied to characterize different onion lines according to the chemical composition of the volatile fraction of nectar and flowers. On the other hand, it was determined which odor components contribute to the pollination of onions lines and relate the content of specific analytes with foraging behavior and seed production. More than 90 compounds were identified. The samples studied showed differences in the volatile profiles of flowers and nectar among the different lines. The results demonstrated that headspace SPME–GCMS is a simple, rapid and solvent-free method suitable for analysis of volatile compounds emitted from onion plants. Furthermore, a great difference was found for the number of bee visits and seed yield among the onion lines analyzed. This study demonstrates that the combination of chemical information and statistical analysis is able to differentiate onion male sterile lines from fertile lines, as well as among male sterile lines. Repellent compounds, such as dioxolanes, piperidines and organosulfurs may contribute negatively to pollination, since the higher content of these analytes, the less bee visits and seed production yield.
publishDate 2016
dc.date.none.fl_str_mv 2016
2017-10-02T14:16:06Z
2017-10-02T14:16:06Z
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/acceptedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str acceptedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/20.500.12123/1375
http://www.sciencedirect.com/science/article/pii/S0026265X15000788
https://doi.org/10.1016/j.microc.2015.04.017
url http://hdl.handle.net/20.500.12123/1375
http://www.sciencedirect.com/science/article/pii/S0026265X15000788
https://doi.org/10.1016/j.microc.2015.04.017
dc.language.none.fl_str_mv eng
language eng
dc.rights.none.fl_str_mv info:eu-repo/semantics/restrictedAccess
eu_rights_str_mv restrictedAccess
dc.format.none.fl_str_mv application/pdf
dc.source.none.fl_str_mv Microchemical journal 122 : 110-118. (September 2015)
reponame:INTA Digital (INTA)
instname:Instituto Nacional de Tecnología Agropecuaria
reponame_str INTA Digital (INTA)
collection INTA Digital (INTA)
instname_str Instituto Nacional de Tecnología Agropecuaria
repository.name.fl_str_mv INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuaria
repository.mail.fl_str_mv tripaldi.nicolas@inta.gob.ar
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score 12.623145

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