Filamentous haemagglutinin-meadiated cell-substrate and cell-cell adhesions are critical for Bordetella pertussis´s biofilm formation on abiotic surfaces and in the mouse nose and...

Autores: Serra, Diego Omar; Conover, Matt S.; Arnal, Laura; Sloan, Gina Parise; Rodriguez, Maria Eugenia; Yantorno, Osvaldo Miguel; Deora, Rajendar
Año de publicación: 2011
Idioma: inglés
Tipo de recurso: artículo
Estado: versión publicada
Descripción: Bordetella spp. form biofilms in the mouse nasopharynx, thereby providing a potential mechanism for establishing chronic infections in humans and animals. Filamentous hemagglutinin (FHA) is a major virulence factor of B. pertussis, the causative agent of the highly transmissible and infectious disease, pertussis. In this study, we dissected the role of FHA in the distinct biofilm developmental stages of B. pertussis on abiotic substrates and in the respiratory tract by employing a murine model of respiratory biofilms. Our results show that the lack of FHA reduced attachment and decreased accumulation of biofilm biomass on artificial surfaces. FHA contributes to biofilm development by promoting the formation of microcolonies. Absence of FHA from B. pertussis or antibody-mediated blockade of surface-associated FHA impaired the attachment of bacteria to the biofilm community. Exogenous addition of FHA resulted in a dose-dependent inhibitory effect on bacterial association with the biofilms. Furthermore, we show that FHA is important for the structural integrity of biofilms formed on the mouse nose and trachea. Together, these results strongly support the hypothesis that FHA promotes the formation and maintenance of biofilms by mediating cell-substrate and inter-bacterial adhesions. These discoveries highlight FHA as a key factor in establishing structured biofilm communities in the respiratory tract.
Fil: Serra, Diego Omar. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Investigación y Desarrollo en Fermentaciones Industriales. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Centro de Investigación y Desarrollo en Fermentaciones Industriales; Argentina
Fil: Conover, Matt S.. Wake Forest University Health Sciences; Estados Unidos
Fil: Arnal, Laura. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Investigación y Desarrollo en Fermentaciones Industriales. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Centro de Investigación y Desarrollo en Fermentaciones Industriales; Argentina
Fil: Sloan, Gina Parise. Wake Forest University Health Sciences; Estados Unidos
Fil: Rodriguez, Maria Eugenia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Investigación y Desarrollo en Fermentaciones Industriales. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Centro de Investigación y Desarrollo en Fermentaciones Industriales; Argentina
Fil: Yantorno, Osvaldo Miguel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Investigación y Desarrollo en Fermentaciones Industriales. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Centro de Investigación y Desarrollo en Fermentaciones Industriales; Argentina
Fil: Deora, Rajendar. Wake Forest University Health Sciences; Estados Unidos
Materia: Bordetella
Biofilm
FHA
Nivel de accesibilidad: acceso abierto
Condiciones de uso: https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
Institución: Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador: oai:ri.conicet.gov.ar:11336/184573

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network_name_str	CONICET Digital (CONICET)
spelling	Filamentous haemagglutinin-meadiated cell-substrate and cell-cell adhesions are critical for Bordetella pertussis´s biofilm formation on abiotic surfaces and in the mouse nose and tracheaSerra, Diego OmarConover, Matt S.Arnal, LauraSloan, Gina PariseRodriguez, Maria EugeniaYantorno, Osvaldo MiguelDeora, RajendarBordetellaBiofilmFHAhttps://purl.org/becyt/ford/3.3https://purl.org/becyt/ford/3Bordetella spp. form biofilms in the mouse nasopharynx, thereby providing a potential mechanism for establishing chronic infections in humans and animals. Filamentous hemagglutinin (FHA) is a major virulence factor of B. pertussis, the causative agent of the highly transmissible and infectious disease, pertussis. In this study, we dissected the role of FHA in the distinct biofilm developmental stages of B. pertussis on abiotic substrates and in the respiratory tract by employing a murine model of respiratory biofilms. Our results show that the lack of FHA reduced attachment and decreased accumulation of biofilm biomass on artificial surfaces. FHA contributes to biofilm development by promoting the formation of microcolonies. Absence of FHA from B. pertussis or antibody-mediated blockade of surface-associated FHA impaired the attachment of bacteria to the biofilm community. Exogenous addition of FHA resulted in a dose-dependent inhibitory effect on bacterial association with the biofilms. Furthermore, we show that FHA is important for the structural integrity of biofilms formed on the mouse nose and trachea. Together, these results strongly support the hypothesis that FHA promotes the formation and maintenance of biofilms by mediating cell-substrate and inter-bacterial adhesions. These discoveries highlight FHA as a key factor in establishing structured biofilm communities in the respiratory tract.Fil: Serra, Diego Omar. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Investigación y Desarrollo en Fermentaciones Industriales. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Centro de Investigación y Desarrollo en Fermentaciones Industriales; ArgentinaFil: Conover, Matt S.. Wake Forest University Health Sciences; Estados UnidosFil: Arnal, Laura. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Investigación y Desarrollo en Fermentaciones Industriales. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Centro de Investigación y Desarrollo en Fermentaciones Industriales; ArgentinaFil: Sloan, Gina Parise. Wake Forest University Health Sciences; Estados UnidosFil: Rodriguez, Maria Eugenia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Investigación y Desarrollo en Fermentaciones Industriales. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Centro de Investigación y Desarrollo en Fermentaciones Industriales; ArgentinaFil: Yantorno, Osvaldo Miguel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Investigación y Desarrollo en Fermentaciones Industriales. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Centro de Investigación y Desarrollo en Fermentaciones Industriales; ArgentinaFil: Deora, Rajendar. Wake Forest University Health Sciences; Estados UnidosPublic Library of Science2011-12info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/184573Serra, Diego Omar; Conover, Matt S.; Arnal, Laura; Sloan, Gina Parise; Rodriguez, Maria Eugenia; et al.; Filamentous haemagglutinin-meadiated cell-substrate and cell-cell adhesions are critical for Bordetella pertussis´s biofilm formation on abiotic surfaces and in the mouse nose and trachea; Public Library of Science; Plos One; 6; 12; 12-2011; 1-151932-6203CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1371/journal.pone.0028811info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T09:41:54Zoai:ri.conicet.gov.ar:11336/184573instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 09:41:54.878CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv	Filamentous haemagglutinin-meadiated cell-substrate and cell-cell adhesions are critical for Bordetella pertussis´s biofilm formation on abiotic surfaces and in the mouse nose and trachea
title	Filamentous haemagglutinin-meadiated cell-substrate and cell-cell adhesions are critical for Bordetella pertussis´s biofilm formation on abiotic surfaces and in the mouse nose and trachea
spellingShingle	Filamentous haemagglutinin-meadiated cell-substrate and cell-cell adhesions are critical for Bordetella pertussis´s biofilm formation on abiotic surfaces and in the mouse nose and trachea Serra, Diego Omar Bordetella Biofilm FHA
title_short	Filamentous haemagglutinin-meadiated cell-substrate and cell-cell adhesions are critical for Bordetella pertussis´s biofilm formation on abiotic surfaces and in the mouse nose and trachea
title_full	Filamentous haemagglutinin-meadiated cell-substrate and cell-cell adhesions are critical for Bordetella pertussis´s biofilm formation on abiotic surfaces and in the mouse nose and trachea
title_fullStr	Filamentous haemagglutinin-meadiated cell-substrate and cell-cell adhesions are critical for Bordetella pertussis´s biofilm formation on abiotic surfaces and in the mouse nose and trachea
title_full_unstemmed	Filamentous haemagglutinin-meadiated cell-substrate and cell-cell adhesions are critical for Bordetella pertussis´s biofilm formation on abiotic surfaces and in the mouse nose and trachea
title_sort	Filamentous haemagglutinin-meadiated cell-substrate and cell-cell adhesions are critical for Bordetella pertussis´s biofilm formation on abiotic surfaces and in the mouse nose and trachea
dc.creator.none.fl_str_mv	Serra, Diego Omar Conover, Matt S. Arnal, Laura Sloan, Gina Parise Rodriguez, Maria Eugenia Yantorno, Osvaldo Miguel Deora, Rajendar
author	Serra, Diego Omar
author_facet	Serra, Diego Omar Conover, Matt S. Arnal, Laura Sloan, Gina Parise Rodriguez, Maria Eugenia Yantorno, Osvaldo Miguel Deora, Rajendar
author_role	author
author2	Conover, Matt S. Arnal, Laura Sloan, Gina Parise Rodriguez, Maria Eugenia Yantorno, Osvaldo Miguel Deora, Rajendar
author2_role	author author author author author author
dc.subject.none.fl_str_mv	Bordetella Biofilm FHA
topic	Bordetella Biofilm FHA
purl_subject.fl_str_mv	https://purl.org/becyt/ford/3.3 https://purl.org/becyt/ford/3
dc.description.none.fl_txt_mv	Bordetella spp. form biofilms in the mouse nasopharynx, thereby providing a potential mechanism for establishing chronic infections in humans and animals. Filamentous hemagglutinin (FHA) is a major virulence factor of B. pertussis, the causative agent of the highly transmissible and infectious disease, pertussis. In this study, we dissected the role of FHA in the distinct biofilm developmental stages of B. pertussis on abiotic substrates and in the respiratory tract by employing a murine model of respiratory biofilms. Our results show that the lack of FHA reduced attachment and decreased accumulation of biofilm biomass on artificial surfaces. FHA contributes to biofilm development by promoting the formation of microcolonies. Absence of FHA from B. pertussis or antibody-mediated blockade of surface-associated FHA impaired the attachment of bacteria to the biofilm community. Exogenous addition of FHA resulted in a dose-dependent inhibitory effect on bacterial association with the biofilms. Furthermore, we show that FHA is important for the structural integrity of biofilms formed on the mouse nose and trachea. Together, these results strongly support the hypothesis that FHA promotes the formation and maintenance of biofilms by mediating cell-substrate and inter-bacterial adhesions. These discoveries highlight FHA as a key factor in establishing structured biofilm communities in the respiratory tract. Fil: Serra, Diego Omar. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Investigación y Desarrollo en Fermentaciones Industriales. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Centro de Investigación y Desarrollo en Fermentaciones Industriales; Argentina Fil: Conover, Matt S.. Wake Forest University Health Sciences; Estados Unidos Fil: Arnal, Laura. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Investigación y Desarrollo en Fermentaciones Industriales. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Centro de Investigación y Desarrollo en Fermentaciones Industriales; Argentina Fil: Sloan, Gina Parise. Wake Forest University Health Sciences; Estados Unidos Fil: Rodriguez, Maria Eugenia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Investigación y Desarrollo en Fermentaciones Industriales. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Centro de Investigación y Desarrollo en Fermentaciones Industriales; Argentina Fil: Yantorno, Osvaldo Miguel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Investigación y Desarrollo en Fermentaciones Industriales. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Centro de Investigación y Desarrollo en Fermentaciones Industriales; Argentina Fil: Deora, Rajendar. Wake Forest University Health Sciences; Estados Unidos
description	Bordetella spp. form biofilms in the mouse nasopharynx, thereby providing a potential mechanism for establishing chronic infections in humans and animals. Filamentous hemagglutinin (FHA) is a major virulence factor of B. pertussis, the causative agent of the highly transmissible and infectious disease, pertussis. In this study, we dissected the role of FHA in the distinct biofilm developmental stages of B. pertussis on abiotic substrates and in the respiratory tract by employing a murine model of respiratory biofilms. Our results show that the lack of FHA reduced attachment and decreased accumulation of biofilm biomass on artificial surfaces. FHA contributes to biofilm development by promoting the formation of microcolonies. Absence of FHA from B. pertussis or antibody-mediated blockade of surface-associated FHA impaired the attachment of bacteria to the biofilm community. Exogenous addition of FHA resulted in a dose-dependent inhibitory effect on bacterial association with the biofilms. Furthermore, we show that FHA is important for the structural integrity of biofilms formed on the mouse nose and trachea. Together, these results strongly support the hypothesis that FHA promotes the formation and maintenance of biofilms by mediating cell-substrate and inter-bacterial adhesions. These discoveries highlight FHA as a key factor in establishing structured biofilm communities in the respiratory tract.
publishDate	2011
dc.date.none.fl_str_mv	2011-12
dc.type.none.fl_str_mv	info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://purl.org/coar/resource_type/c_6501 info:ar-repo/semantics/articulo
format	article
status_str	publishedVersion
dc.identifier.none.fl_str_mv	http://hdl.handle.net/11336/184573 Serra, Diego Omar; Conover, Matt S.; Arnal, Laura; Sloan, Gina Parise; Rodriguez, Maria Eugenia; et al.; Filamentous haemagglutinin-meadiated cell-substrate and cell-cell adhesions are critical for Bordetella pertussis´s biofilm formation on abiotic surfaces and in the mouse nose and trachea; Public Library of Science; Plos One; 6; 12; 12-2011; 1-15 1932-6203 CONICET Digital CONICET
url	http://hdl.handle.net/11336/184573
identifier_str_mv	Serra, Diego Omar; Conover, Matt S.; Arnal, Laura; Sloan, Gina Parise; Rodriguez, Maria Eugenia; et al.; Filamentous haemagglutinin-meadiated cell-substrate and cell-cell adhesions are critical for Bordetella pertussis´s biofilm formation on abiotic surfaces and in the mouse nose and trachea; Public Library of Science; Plos One; 6; 12; 12-2011; 1-15 1932-6203 CONICET Digital CONICET
dc.language.none.fl_str_mv	eng
language	eng
dc.relation.none.fl_str_mv	info:eu-repo/semantics/altIdentifier/doi/10.1371/journal.pone.0028811
dc.rights.none.fl_str_mv	info:eu-repo/semantics/openAccess https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv	openAccess
rights_invalid_str_mv	https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv	application/pdf application/pdf
dc.publisher.none.fl_str_mv	Public Library of Science
publisher.none.fl_str_mv	Public Library of Science
dc.source.none.fl_str_mv	reponame:CONICET Digital (CONICET) instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str	CONICET Digital (CONICET)
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repository.name.fl_str_mv	CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv	dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score	13.070432

Filamentous haemagglutinin-meadiated cell-substrate and cell-cell adhesions are critical for Bordetella pertussis´s biofilm formation on abiotic surfaces and in the mouse nose and...

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