Effect of hydromechanical forces on the production of filamentous haemagglutinin and pertussis toxin of Bordetella pertussis

Autores
Rodríguez, María Eugenia; Samo, Analia L.; Hozbor, Daniela Flavia; Yantorno, Osvaldo Miguel
Año de publicación
1993
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The production of Bordetella pertussis extracytoplasmic filamentous haemagglutinin (FHA) and pertussis toxin (PT) in a bioreactor under stirring conditions was studied in order to investigate the effect of hydromechanical forces on yields of both antigens. It was shown that FHA loses its haemagglutinin activity when the power transmitted by the agitator and the aerator per unit volume increases, whereas PT production is not affected. The loss of FHA activity can be explained by the action of shear forces on the filamentous structure of this antigen.
Centro de Investigación y Desarrollo en Fermentaciones Industriales
Materia
Química
Física
Bordetella pertussis
Filamentous haemagglutinin
Pertussis toxin
Production
Shear stress
Bioreactor
Nivel de accesibilidad
acceso abierto
Condiciones de uso
http://creativecommons.org/licenses/by/4.0/
Repositorio
SEDICI (UNLP)
Institución
Universidad Nacional de La Plata
OAI Identificador
oai:sedici.unlp.edu.ar:10915/139310
Acceder
id SEDICI_c2c77c26ce5bd160bb8ba2ce5506090a
oai_identifier_str oai:sedici.unlp.edu.ar:10915/139310
network_acronym_str SEDICI
repository_id_str 1329
network_name_str SEDICI (UNLP)
spelling Effect of hydromechanical forces on the production of filamentous haemagglutinin and pertussis toxin of Bordetella pertussisRodríguez, María EugeniaSamo, Analia L.Hozbor, Daniela FlaviaYantorno, Osvaldo MiguelQuímicaFísicaBordetella pertussisFilamentous haemagglutininPertussis toxinProductionShear stressBioreactorThe production of Bordetella pertussis extracytoplasmic filamentous haemagglutinin (FHA) and pertussis toxin (PT) in a bioreactor under stirring conditions was studied in order to investigate the effect of hydromechanical forces on yields of both antigens. It was shown that FHA loses its haemagglutinin activity when the power transmitted by the agitator and the aerator per unit volume increases, whereas PT production is not affected. The loss of FHA activity can be explained by the action of shear forces on the filamentous structure of this antigen.Centro de Investigación y Desarrollo en Fermentaciones Industriales1993info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionArticulohttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdf103-108http://sedici.unlp.edu.ar/handle/10915/139310enginfo:eu-repo/semantics/altIdentifier/issn/0169-4146info:eu-repo/semantics/altIdentifier/issn/1476-5535info:eu-repo/semantics/altIdentifier/issn/1367-5435info:eu-repo/semantics/altIdentifier/doi/10.1007/bf01569909info:eu-repo/semantics/altIdentifier/pmid/7764157info:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by/4.0/Creative Commons Attribution 4.0 International (CC BY 4.0)reponame:SEDICI (UNLP)instname:Universidad Nacional de La Platainstacron:UNLP2025-10-22T17:12:56Zoai:sedici.unlp.edu.ar:10915/139310Institucionalhttp://sedici.unlp.edu.ar/Universidad públicaNo correspondehttp://sedici.unlp.edu.ar/oai/snrdalira@sedici.unlp.edu.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:13292025-10-22 17:12:56.651SEDICI (UNLP) - Universidad Nacional de La Platafalse
dc.title.none.fl_str_mv Effect of hydromechanical forces on the production of filamentous haemagglutinin and pertussis toxin of Bordetella pertussis
title Effect of hydromechanical forces on the production of filamentous haemagglutinin and pertussis toxin of Bordetella pertussis
spellingShingle Effect of hydromechanical forces on the production of filamentous haemagglutinin and pertussis toxin of Bordetella pertussis
Rodríguez, María Eugenia
Química
Física
Bordetella pertussis
Filamentous haemagglutinin
Pertussis toxin
Production
Shear stress
Bioreactor
title_short Effect of hydromechanical forces on the production of filamentous haemagglutinin and pertussis toxin of Bordetella pertussis
title_full Effect of hydromechanical forces on the production of filamentous haemagglutinin and pertussis toxin of Bordetella pertussis
title_fullStr Effect of hydromechanical forces on the production of filamentous haemagglutinin and pertussis toxin of Bordetella pertussis
title_full_unstemmed Effect of hydromechanical forces on the production of filamentous haemagglutinin and pertussis toxin of Bordetella pertussis
title_sort Effect of hydromechanical forces on the production of filamentous haemagglutinin and pertussis toxin of Bordetella pertussis
dc.creator.none.fl_str_mv Rodríguez, María Eugenia
Samo, Analia L.
Hozbor, Daniela Flavia
Yantorno, Osvaldo Miguel
author Rodríguez, María Eugenia
author_facet Rodríguez, María Eugenia
Samo, Analia L.
Hozbor, Daniela Flavia
Yantorno, Osvaldo Miguel
author_role author
author2 Samo, Analia L.
Hozbor, Daniela Flavia
Yantorno, Osvaldo Miguel
author2_role author
author
author
dc.subject.none.fl_str_mv Química
Física
Bordetella pertussis
Filamentous haemagglutinin
Pertussis toxin
Production
Shear stress
Bioreactor
topic Química
Física
Bordetella pertussis
Filamentous haemagglutinin
Pertussis toxin
Production
Shear stress
Bioreactor
dc.description.none.fl_txt_mv The production of Bordetella pertussis extracytoplasmic filamentous haemagglutinin (FHA) and pertussis toxin (PT) in a bioreactor under stirring conditions was studied in order to investigate the effect of hydromechanical forces on yields of both antigens. It was shown that FHA loses its haemagglutinin activity when the power transmitted by the agitator and the aerator per unit volume increases, whereas PT production is not affected. The loss of FHA activity can be explained by the action of shear forces on the filamentous structure of this antigen.
Centro de Investigación y Desarrollo en Fermentaciones Industriales
description The production of Bordetella pertussis extracytoplasmic filamentous haemagglutinin (FHA) and pertussis toxin (PT) in a bioreactor under stirring conditions was studied in order to investigate the effect of hydromechanical forces on yields of both antigens. It was shown that FHA loses its haemagglutinin activity when the power transmitted by the agitator and the aerator per unit volume increases, whereas PT production is not affected. The loss of FHA activity can be explained by the action of shear forces on the filamentous structure of this antigen.
publishDate 1993
dc.date.none.fl_str_mv 1993
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Articulo
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://sedici.unlp.edu.ar/handle/10915/139310
url http://sedici.unlp.edu.ar/handle/10915/139310
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/issn/0169-4146
info:eu-repo/semantics/altIdentifier/issn/1476-5535
info:eu-repo/semantics/altIdentifier/issn/1367-5435
info:eu-repo/semantics/altIdentifier/doi/10.1007/bf01569909
info:eu-repo/semantics/altIdentifier/pmid/7764157
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
http://creativecommons.org/licenses/by/4.0/
Creative Commons Attribution 4.0 International (CC BY 4.0)
eu_rights_str_mv openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by/4.0/
Creative Commons Attribution 4.0 International (CC BY 4.0)
dc.format.none.fl_str_mv application/pdf
103-108
dc.source.none.fl_str_mv reponame:SEDICI (UNLP)
instname:Universidad Nacional de La Plata
instacron:UNLP
reponame_str SEDICI (UNLP)
collection SEDICI (UNLP)
instname_str Universidad Nacional de La Plata
instacron_str UNLP
institution UNLP
repository.name.fl_str_mv SEDICI (UNLP) - Universidad Nacional de La Plata
repository.mail.fl_str_mv alira@sedici.unlp.edu.ar
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score 12.982451

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