Mitochondrial metabolism and respiration adjustments following temperature acclimation in Daphnia magna
- Autores
- Hoffschröer, Nadine; Laspoumaderes, Cecilia; Zeis, Bettina; Tremblay, Nelly
- Año de publicación
- 2024
- Idioma
- inglés
- Tipo de recurso
- artículo
- Estado
- versión publicada
- Descripción
- Seasonal temperature changes and local variations in the water column challenge lentic zooplankton in their habitat. At the cellular level, exposure to varying temperatures affects the mitochondrial functional properties of poikilothermic organisms. Metabolic enzymes that supply reduced substrates to the electron transport chain and elements of the oxidative phosphorylation system must therefore adjust their activity and flux rates to the altered temperature conditions. In the present study, Daphnia magna respiration was analyzed in response to acute and chronic changes in ambient temperature. Oxygen consumption as well as substrate and electron flux rates were measured at the animals´ acclimation temperature and at two additional acute temperatures. High activity of citrate synthase (CS) in cold-acclimated animals (10 °C) may have resulted from mitochondrial quantitative adjustments. However, thermal sensitivity of the functional properties of mitochondrial enzymes was greater in warm-acclimated animals (30 °C). In whole animals, temperature-induced changes were partly compensated by acclimation, but these changes were promoted by acclimation in the case of the mitochondrial electron transport chain. Thus, respiration realised in whole animals was limited by the provision of reduced substrates in the tricarboxylic acid cycle rather than by restrictions of the respiratory chain complexes. This may minimize production of reactive oxygen species and resulting damage and reduce waste of substrates from the animals’ energy reserves. Still, the integrated biomarker response indicated increased defense against oxidative stress at elevated temperatures.
Fil: Hoffschröer, Nadine. Institut Für Integrative Zellbiologie Und Physiologie; Alemania
Fil: Laspoumaderes, Cecilia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Patagonia Norte. Instituto de Investigaciones en Biodiversidad y Medioambiente. Universidad Nacional del Comahue. Centro Regional Universidad Bariloche. Instituto de Investigaciones en Biodiversidad y Medioambiente; Argentina
Fil: Zeis, Bettina. Institut Für Integrative Zellbiologie Und Physiologie; Alemania
Fil: Tremblay, Nelly. Alfred-Wegener-Institut, Helmholtz-Zentrum für Polar- und Meeresforschung; Alemania - Materia
-
MITHOCONDRIA
TEMPERATURE ACCLIMATION
OROBOROS
OXPHOS
RESOIRATION
METABOLISM - Nivel de accesibilidad
- acceso abierto
- Condiciones de uso
- https://creativecommons.org/licenses/by-nc/2.5/ar/
- Repositorio
- Institución
- Consejo Nacional de Investigaciones Científicas y Técnicas
- OAI Identificador
- oai:ri.conicet.gov.ar:11336/265219
Ver los metadatos del registro completo
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Mitochondrial metabolism and respiration adjustments following temperature acclimation in Daphnia magnaHoffschröer, NadineLaspoumaderes, CeciliaZeis, BettinaTremblay, NellyMITHOCONDRIATEMPERATURE ACCLIMATIONOROBOROSOXPHOSRESOIRATIONMETABOLISMhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Seasonal temperature changes and local variations in the water column challenge lentic zooplankton in their habitat. At the cellular level, exposure to varying temperatures affects the mitochondrial functional properties of poikilothermic organisms. Metabolic enzymes that supply reduced substrates to the electron transport chain and elements of the oxidative phosphorylation system must therefore adjust their activity and flux rates to the altered temperature conditions. In the present study, Daphnia magna respiration was analyzed in response to acute and chronic changes in ambient temperature. Oxygen consumption as well as substrate and electron flux rates were measured at the animals´ acclimation temperature and at two additional acute temperatures. High activity of citrate synthase (CS) in cold-acclimated animals (10 °C) may have resulted from mitochondrial quantitative adjustments. However, thermal sensitivity of the functional properties of mitochondrial enzymes was greater in warm-acclimated animals (30 °C). In whole animals, temperature-induced changes were partly compensated by acclimation, but these changes were promoted by acclimation in the case of the mitochondrial electron transport chain. Thus, respiration realised in whole animals was limited by the provision of reduced substrates in the tricarboxylic acid cycle rather than by restrictions of the respiratory chain complexes. This may minimize production of reactive oxygen species and resulting damage and reduce waste of substrates from the animals’ energy reserves. Still, the integrated biomarker response indicated increased defense against oxidative stress at elevated temperatures.Fil: Hoffschröer, Nadine. Institut Für Integrative Zellbiologie Und Physiologie; AlemaniaFil: Laspoumaderes, Cecilia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Patagonia Norte. Instituto de Investigaciones en Biodiversidad y Medioambiente. Universidad Nacional del Comahue. Centro Regional Universidad Bariloche. Instituto de Investigaciones en Biodiversidad y Medioambiente; ArgentinaFil: Zeis, Bettina. Institut Für Integrative Zellbiologie Und Physiologie; AlemaniaFil: Tremblay, Nelly. Alfred-Wegener-Institut, Helmholtz-Zentrum für Polar- und Meeresforschung; AlemaniaPergamon-Elsevier Science Ltd2024-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/265219Hoffschröer, Nadine; Laspoumaderes, Cecilia; Zeis, Bettina; Tremblay, Nelly; Mitochondrial metabolism and respiration adjustments following temperature acclimation in Daphnia magna; Pergamon-Elsevier Science Ltd; Journal of Thermal Biology; 119; 1-2024; 1-120306-4565CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1016/j.jtherbio.2023.103761info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:21:25Zoai:ri.conicet.gov.ar:11336/265219instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:21:25.805CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse |
dc.title.none.fl_str_mv |
Mitochondrial metabolism and respiration adjustments following temperature acclimation in Daphnia magna |
title |
Mitochondrial metabolism and respiration adjustments following temperature acclimation in Daphnia magna |
spellingShingle |
Mitochondrial metabolism and respiration adjustments following temperature acclimation in Daphnia magna Hoffschröer, Nadine MITHOCONDRIA TEMPERATURE ACCLIMATION OROBOROS OXPHOS RESOIRATION METABOLISM |
title_short |
Mitochondrial metabolism and respiration adjustments following temperature acclimation in Daphnia magna |
title_full |
Mitochondrial metabolism and respiration adjustments following temperature acclimation in Daphnia magna |
title_fullStr |
Mitochondrial metabolism and respiration adjustments following temperature acclimation in Daphnia magna |
title_full_unstemmed |
Mitochondrial metabolism and respiration adjustments following temperature acclimation in Daphnia magna |
title_sort |
Mitochondrial metabolism and respiration adjustments following temperature acclimation in Daphnia magna |
dc.creator.none.fl_str_mv |
Hoffschröer, Nadine Laspoumaderes, Cecilia Zeis, Bettina Tremblay, Nelly |
author |
Hoffschröer, Nadine |
author_facet |
Hoffschröer, Nadine Laspoumaderes, Cecilia Zeis, Bettina Tremblay, Nelly |
author_role |
author |
author2 |
Laspoumaderes, Cecilia Zeis, Bettina Tremblay, Nelly |
author2_role |
author author author |
dc.subject.none.fl_str_mv |
MITHOCONDRIA TEMPERATURE ACCLIMATION OROBOROS OXPHOS RESOIRATION METABOLISM |
topic |
MITHOCONDRIA TEMPERATURE ACCLIMATION OROBOROS OXPHOS RESOIRATION METABOLISM |
purl_subject.fl_str_mv |
https://purl.org/becyt/ford/1.6 https://purl.org/becyt/ford/1 |
dc.description.none.fl_txt_mv |
Seasonal temperature changes and local variations in the water column challenge lentic zooplankton in their habitat. At the cellular level, exposure to varying temperatures affects the mitochondrial functional properties of poikilothermic organisms. Metabolic enzymes that supply reduced substrates to the electron transport chain and elements of the oxidative phosphorylation system must therefore adjust their activity and flux rates to the altered temperature conditions. In the present study, Daphnia magna respiration was analyzed in response to acute and chronic changes in ambient temperature. Oxygen consumption as well as substrate and electron flux rates were measured at the animals´ acclimation temperature and at two additional acute temperatures. High activity of citrate synthase (CS) in cold-acclimated animals (10 °C) may have resulted from mitochondrial quantitative adjustments. However, thermal sensitivity of the functional properties of mitochondrial enzymes was greater in warm-acclimated animals (30 °C). In whole animals, temperature-induced changes were partly compensated by acclimation, but these changes were promoted by acclimation in the case of the mitochondrial electron transport chain. Thus, respiration realised in whole animals was limited by the provision of reduced substrates in the tricarboxylic acid cycle rather than by restrictions of the respiratory chain complexes. This may minimize production of reactive oxygen species and resulting damage and reduce waste of substrates from the animals’ energy reserves. Still, the integrated biomarker response indicated increased defense against oxidative stress at elevated temperatures. Fil: Hoffschröer, Nadine. Institut Für Integrative Zellbiologie Und Physiologie; Alemania Fil: Laspoumaderes, Cecilia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Patagonia Norte. Instituto de Investigaciones en Biodiversidad y Medioambiente. Universidad Nacional del Comahue. Centro Regional Universidad Bariloche. Instituto de Investigaciones en Biodiversidad y Medioambiente; Argentina Fil: Zeis, Bettina. Institut Für Integrative Zellbiologie Und Physiologie; Alemania Fil: Tremblay, Nelly. Alfred-Wegener-Institut, Helmholtz-Zentrum für Polar- und Meeresforschung; Alemania |
description |
Seasonal temperature changes and local variations in the water column challenge lentic zooplankton in their habitat. At the cellular level, exposure to varying temperatures affects the mitochondrial functional properties of poikilothermic organisms. Metabolic enzymes that supply reduced substrates to the electron transport chain and elements of the oxidative phosphorylation system must therefore adjust their activity and flux rates to the altered temperature conditions. In the present study, Daphnia magna respiration was analyzed in response to acute and chronic changes in ambient temperature. Oxygen consumption as well as substrate and electron flux rates were measured at the animals´ acclimation temperature and at two additional acute temperatures. High activity of citrate synthase (CS) in cold-acclimated animals (10 °C) may have resulted from mitochondrial quantitative adjustments. However, thermal sensitivity of the functional properties of mitochondrial enzymes was greater in warm-acclimated animals (30 °C). In whole animals, temperature-induced changes were partly compensated by acclimation, but these changes were promoted by acclimation in the case of the mitochondrial electron transport chain. Thus, respiration realised in whole animals was limited by the provision of reduced substrates in the tricarboxylic acid cycle rather than by restrictions of the respiratory chain complexes. This may minimize production of reactive oxygen species and resulting damage and reduce waste of substrates from the animals’ energy reserves. Still, the integrated biomarker response indicated increased defense against oxidative stress at elevated temperatures. |
publishDate |
2024 |
dc.date.none.fl_str_mv |
2024-01 |
dc.type.none.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://purl.org/coar/resource_type/c_6501 info:ar-repo/semantics/articulo |
format |
article |
status_str |
publishedVersion |
dc.identifier.none.fl_str_mv |
http://hdl.handle.net/11336/265219 Hoffschröer, Nadine; Laspoumaderes, Cecilia; Zeis, Bettina; Tremblay, Nelly; Mitochondrial metabolism and respiration adjustments following temperature acclimation in Daphnia magna; Pergamon-Elsevier Science Ltd; Journal of Thermal Biology; 119; 1-2024; 1-12 0306-4565 CONICET Digital CONICET |
url |
http://hdl.handle.net/11336/265219 |
identifier_str_mv |
Hoffschröer, Nadine; Laspoumaderes, Cecilia; Zeis, Bettina; Tremblay, Nelly; Mitochondrial metabolism and respiration adjustments following temperature acclimation in Daphnia magna; Pergamon-Elsevier Science Ltd; Journal of Thermal Biology; 119; 1-2024; 1-12 0306-4565 CONICET Digital CONICET |
dc.language.none.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
info:eu-repo/semantics/altIdentifier/doi/10.1016/j.jtherbio.2023.103761 |
dc.rights.none.fl_str_mv |
info:eu-repo/semantics/openAccess https://creativecommons.org/licenses/by-nc/2.5/ar/ |
eu_rights_str_mv |
openAccess |
rights_invalid_str_mv |
https://creativecommons.org/licenses/by-nc/2.5/ar/ |
dc.format.none.fl_str_mv |
application/pdf application/pdf |
dc.publisher.none.fl_str_mv |
Pergamon-Elsevier Science Ltd |
publisher.none.fl_str_mv |
Pergamon-Elsevier Science Ltd |
dc.source.none.fl_str_mv |
reponame:CONICET Digital (CONICET) instname:Consejo Nacional de Investigaciones Científicas y Técnicas |
reponame_str |
CONICET Digital (CONICET) |
collection |
CONICET Digital (CONICET) |
instname_str |
Consejo Nacional de Investigaciones Científicas y Técnicas |
repository.name.fl_str_mv |
CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas |
repository.mail.fl_str_mv |
dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar |
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1844614202599145472 |
score |
13.070432 |