Mitochondrial metabolism and respiration adjustments following temperature acclimation in Daphnia magna

Autores
Hoffschröer, Nadine; Laspoumaderes, Cecilia; Zeis, Bettina; Tremblay, Nelly
Año de publicación
2024
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Seasonal temperature changes and local variations in the water column challenge lentic zooplankton in their habitat. At the cellular level, exposure to varying temperatures affects the mitochondrial functional properties of poikilothermic organisms. Metabolic enzymes that supply reduced substrates to the electron transport chain and elements of the oxidative phosphorylation system must therefore adjust their activity and flux rates to the altered temperature conditions. In the present study, Daphnia magna respiration was analyzed in response to acute and chronic changes in ambient temperature. Oxygen consumption as well as substrate and electron flux rates were measured at the animals´ acclimation temperature and at two additional acute temperatures. High activity of citrate synthase (CS) in cold-acclimated animals (10 °C) may have resulted from mitochondrial quantitative adjustments. However, thermal sensitivity of the functional properties of mitochondrial enzymes was greater in warm-acclimated animals (30 °C). In whole animals, temperature-induced changes were partly compensated by acclimation, but these changes were promoted by acclimation in the case of the mitochondrial electron transport chain. Thus, respiration realised in whole animals was limited by the provision of reduced substrates in the tricarboxylic acid cycle rather than by restrictions of the respiratory chain complexes. This may minimize production of reactive oxygen species and resulting damage and reduce waste of substrates from the animals’ energy reserves. Still, the integrated biomarker response indicated increased defense against oxidative stress at elevated temperatures.
Fil: Hoffschröer, Nadine. Institut Für Integrative Zellbiologie Und Physiologie; Alemania
Fil: Laspoumaderes, Cecilia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Patagonia Norte. Instituto de Investigaciones en Biodiversidad y Medioambiente. Universidad Nacional del Comahue. Centro Regional Universidad Bariloche. Instituto de Investigaciones en Biodiversidad y Medioambiente; Argentina
Fil: Zeis, Bettina. Institut Für Integrative Zellbiologie Und Physiologie; Alemania
Fil: Tremblay, Nelly. Alfred-Wegener-Institut, Helmholtz-Zentrum für Polar- und Meeresforschung; Alemania
Materia
MITHOCONDRIA
TEMPERATURE ACCLIMATION
OROBOROS
OXPHOS
RESOIRATION
METABOLISM
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/265219
Acceder
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network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Mitochondrial metabolism and respiration adjustments following temperature acclimation in Daphnia magnaHoffschröer, NadineLaspoumaderes, CeciliaZeis, BettinaTremblay, NellyMITHOCONDRIATEMPERATURE ACCLIMATIONOROBOROSOXPHOSRESOIRATIONMETABOLISMhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Seasonal temperature changes and local variations in the water column challenge lentic zooplankton in their habitat. At the cellular level, exposure to varying temperatures affects the mitochondrial functional properties of poikilothermic organisms. Metabolic enzymes that supply reduced substrates to the electron transport chain and elements of the oxidative phosphorylation system must therefore adjust their activity and flux rates to the altered temperature conditions. In the present study, Daphnia magna respiration was analyzed in response to acute and chronic changes in ambient temperature. Oxygen consumption as well as substrate and electron flux rates were measured at the animals´ acclimation temperature and at two additional acute temperatures. High activity of citrate synthase (CS) in cold-acclimated animals (10 °C) may have resulted from mitochondrial quantitative adjustments. However, thermal sensitivity of the functional properties of mitochondrial enzymes was greater in warm-acclimated animals (30 °C). In whole animals, temperature-induced changes were partly compensated by acclimation, but these changes were promoted by acclimation in the case of the mitochondrial electron transport chain. Thus, respiration realised in whole animals was limited by the provision of reduced substrates in the tricarboxylic acid cycle rather than by restrictions of the respiratory chain complexes. This may minimize production of reactive oxygen species and resulting damage and reduce waste of substrates from the animals’ energy reserves. Still, the integrated biomarker response indicated increased defense against oxidative stress at elevated temperatures.Fil: Hoffschröer, Nadine. Institut Für Integrative Zellbiologie Und Physiologie; AlemaniaFil: Laspoumaderes, Cecilia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Patagonia Norte. Instituto de Investigaciones en Biodiversidad y Medioambiente. Universidad Nacional del Comahue. Centro Regional Universidad Bariloche. Instituto de Investigaciones en Biodiversidad y Medioambiente; ArgentinaFil: Zeis, Bettina. Institut Für Integrative Zellbiologie Und Physiologie; AlemaniaFil: Tremblay, Nelly. Alfred-Wegener-Institut, Helmholtz-Zentrum für Polar- und Meeresforschung; AlemaniaPergamon-Elsevier Science Ltd2024-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/265219Hoffschröer, Nadine; Laspoumaderes, Cecilia; Zeis, Bettina; Tremblay, Nelly; Mitochondrial metabolism and respiration adjustments following temperature acclimation in Daphnia magna; Pergamon-Elsevier Science Ltd; Journal of Thermal Biology; 119; 1-2024; 1-120306-4565CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1016/j.jtherbio.2023.103761info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-15T15:18:33Zoai:ri.conicet.gov.ar:11336/265219instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-15 15:18:34.157CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Mitochondrial metabolism and respiration adjustments following temperature acclimation in Daphnia magna
title Mitochondrial metabolism and respiration adjustments following temperature acclimation in Daphnia magna
spellingShingle Mitochondrial metabolism and respiration adjustments following temperature acclimation in Daphnia magna
Hoffschröer, Nadine
MITHOCONDRIA
TEMPERATURE ACCLIMATION
OROBOROS
OXPHOS
RESOIRATION
METABOLISM
title_short Mitochondrial metabolism and respiration adjustments following temperature acclimation in Daphnia magna
title_full Mitochondrial metabolism and respiration adjustments following temperature acclimation in Daphnia magna
title_fullStr Mitochondrial metabolism and respiration adjustments following temperature acclimation in Daphnia magna
title_full_unstemmed Mitochondrial metabolism and respiration adjustments following temperature acclimation in Daphnia magna
title_sort Mitochondrial metabolism and respiration adjustments following temperature acclimation in Daphnia magna
dc.creator.none.fl_str_mv Hoffschröer, Nadine
Laspoumaderes, Cecilia
Zeis, Bettina
Tremblay, Nelly
author Hoffschröer, Nadine
author_facet Hoffschröer, Nadine
Laspoumaderes, Cecilia
Zeis, Bettina
Tremblay, Nelly
author_role author
author2 Laspoumaderes, Cecilia
Zeis, Bettina
Tremblay, Nelly
author2_role author
author
author
dc.subject.none.fl_str_mv MITHOCONDRIA
TEMPERATURE ACCLIMATION
OROBOROS
OXPHOS
RESOIRATION
METABOLISM
topic MITHOCONDRIA
TEMPERATURE ACCLIMATION
OROBOROS
OXPHOS
RESOIRATION
METABOLISM
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Seasonal temperature changes and local variations in the water column challenge lentic zooplankton in their habitat. At the cellular level, exposure to varying temperatures affects the mitochondrial functional properties of poikilothermic organisms. Metabolic enzymes that supply reduced substrates to the electron transport chain and elements of the oxidative phosphorylation system must therefore adjust their activity and flux rates to the altered temperature conditions. In the present study, Daphnia magna respiration was analyzed in response to acute and chronic changes in ambient temperature. Oxygen consumption as well as substrate and electron flux rates were measured at the animals´ acclimation temperature and at two additional acute temperatures. High activity of citrate synthase (CS) in cold-acclimated animals (10 °C) may have resulted from mitochondrial quantitative adjustments. However, thermal sensitivity of the functional properties of mitochondrial enzymes was greater in warm-acclimated animals (30 °C). In whole animals, temperature-induced changes were partly compensated by acclimation, but these changes were promoted by acclimation in the case of the mitochondrial electron transport chain. Thus, respiration realised in whole animals was limited by the provision of reduced substrates in the tricarboxylic acid cycle rather than by restrictions of the respiratory chain complexes. This may minimize production of reactive oxygen species and resulting damage and reduce waste of substrates from the animals’ energy reserves. Still, the integrated biomarker response indicated increased defense against oxidative stress at elevated temperatures.
Fil: Hoffschröer, Nadine. Institut Für Integrative Zellbiologie Und Physiologie; Alemania
Fil: Laspoumaderes, Cecilia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Patagonia Norte. Instituto de Investigaciones en Biodiversidad y Medioambiente. Universidad Nacional del Comahue. Centro Regional Universidad Bariloche. Instituto de Investigaciones en Biodiversidad y Medioambiente; Argentina
Fil: Zeis, Bettina. Institut Für Integrative Zellbiologie Und Physiologie; Alemania
Fil: Tremblay, Nelly. Alfred-Wegener-Institut, Helmholtz-Zentrum für Polar- und Meeresforschung; Alemania
description Seasonal temperature changes and local variations in the water column challenge lentic zooplankton in their habitat. At the cellular level, exposure to varying temperatures affects the mitochondrial functional properties of poikilothermic organisms. Metabolic enzymes that supply reduced substrates to the electron transport chain and elements of the oxidative phosphorylation system must therefore adjust their activity and flux rates to the altered temperature conditions. In the present study, Daphnia magna respiration was analyzed in response to acute and chronic changes in ambient temperature. Oxygen consumption as well as substrate and electron flux rates were measured at the animals´ acclimation temperature and at two additional acute temperatures. High activity of citrate synthase (CS) in cold-acclimated animals (10 °C) may have resulted from mitochondrial quantitative adjustments. However, thermal sensitivity of the functional properties of mitochondrial enzymes was greater in warm-acclimated animals (30 °C). In whole animals, temperature-induced changes were partly compensated by acclimation, but these changes were promoted by acclimation in the case of the mitochondrial electron transport chain. Thus, respiration realised in whole animals was limited by the provision of reduced substrates in the tricarboxylic acid cycle rather than by restrictions of the respiratory chain complexes. This may minimize production of reactive oxygen species and resulting damage and reduce waste of substrates from the animals’ energy reserves. Still, the integrated biomarker response indicated increased defense against oxidative stress at elevated temperatures.
publishDate 2024
dc.date.none.fl_str_mv 2024-01
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/265219
Hoffschröer, Nadine; Laspoumaderes, Cecilia; Zeis, Bettina; Tremblay, Nelly; Mitochondrial metabolism and respiration adjustments following temperature acclimation in Daphnia magna; Pergamon-Elsevier Science Ltd; Journal of Thermal Biology; 119; 1-2024; 1-12
0306-4565
CONICET Digital
CONICET
url http://hdl.handle.net/11336/265219
identifier_str_mv Hoffschröer, Nadine; Laspoumaderes, Cecilia; Zeis, Bettina; Tremblay, Nelly; Mitochondrial metabolism and respiration adjustments following temperature acclimation in Daphnia magna; Pergamon-Elsevier Science Ltd; Journal of Thermal Biology; 119; 1-2024; 1-12
0306-4565
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1016/j.jtherbio.2023.103761
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Pergamon-Elsevier Science Ltd
publisher.none.fl_str_mv Pergamon-Elsevier Science Ltd
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1846083334510215168
score 13.22299

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