Effect of the acclimation of a Streptomyces consortium on lindane biodegradation by free and immobilized cells

Autores
Sáez, Juliana María; Aparicio, Juan Daniel; Amoroso, Maria Julia del R.; Benimeli, Claudia Susana
Año de publicación
2015
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Lindane is an organochlorine pesticide widely used in agriculture, human health, and veterinary. It is a persistent organic pollutant, representing a serious risk since its residues are still in the environment. The aim of this work was to evaluate the influence of the acclimation of free and immobilized Streptomyces consortium on lindane degradation in liquid and slurry systems. The actinobacteria consortium demonstrated stability since the survival of all members was confirmed after the acclimation period. It reached higher biomass (0.56-0.65 g L-1) and lindane removal (40-97%) than the consortium without acclimation (0.37-0.44 g L-1; 33-87%) when it was cultured in liquid medium with 20 and 50 mg L-1 of lindane. In contrast, lindane removal achieved by acclimated and immobilized consortium in slurry system (50%) did not present significant difference respect to the consortium without acclimation. Moreover, lindane degradation was confirmed by identification of three metabolites in liquid medium, 1,2- and 1,4-dichlorobenzene, and γ-pentachlorocyclohexene; and by determination of chloride ions released in slurry system. Bioassays with Lactuca sativa demonstrated the effectiveness of the bioremediation by the acclimated consortium in both systems, since the seedlings showed an improvement in its biological parameters compared to abiotic controls, confirming a significant decrease in toxicity.
Fil: Sáez, Juliana María. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina
Fil: Aparicio, Juan Daniel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina
Fil: Amoroso, Maria Julia del R.. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina. Universidad del Norte Santo Tomás de Aquino; Argentina. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia; Argentina
Fil: Benimeli, Claudia Susana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina. Universidad del Norte Santo Tomás de Aquino; Argentina
Materia
ACCLIMATION
BIODEGRADATION
CONSORTIUM
IMMBILIZATION
LINDANE
STREPTOMYCES
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/111607
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/111607
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Effect of the acclimation of a Streptomyces consortium on lindane biodegradation by free and immobilized cellsSáez, Juliana MaríaAparicio, Juan DanielAmoroso, Maria Julia del R.Benimeli, Claudia SusanaACCLIMATIONBIODEGRADATIONCONSORTIUMIMMBILIZATIONLINDANESTREPTOMYCEShttps://purl.org/becyt/ford/2.8https://purl.org/becyt/ford/2Lindane is an organochlorine pesticide widely used in agriculture, human health, and veterinary. It is a persistent organic pollutant, representing a serious risk since its residues are still in the environment. The aim of this work was to evaluate the influence of the acclimation of free and immobilized Streptomyces consortium on lindane degradation in liquid and slurry systems. The actinobacteria consortium demonstrated stability since the survival of all members was confirmed after the acclimation period. It reached higher biomass (0.56-0.65 g L-1) and lindane removal (40-97%) than the consortium without acclimation (0.37-0.44 g L-1; 33-87%) when it was cultured in liquid medium with 20 and 50 mg L-1 of lindane. In contrast, lindane removal achieved by acclimated and immobilized consortium in slurry system (50%) did not present significant difference respect to the consortium without acclimation. Moreover, lindane degradation was confirmed by identification of three metabolites in liquid medium, 1,2- and 1,4-dichlorobenzene, and γ-pentachlorocyclohexene; and by determination of chloride ions released in slurry system. Bioassays with Lactuca sativa demonstrated the effectiveness of the bioremediation by the acclimated consortium in both systems, since the seedlings showed an improvement in its biological parameters compared to abiotic controls, confirming a significant decrease in toxicity.Fil: Sáez, Juliana María. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; ArgentinaFil: Aparicio, Juan Daniel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; ArgentinaFil: Amoroso, Maria Julia del R.. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina. Universidad del Norte Santo Tomás de Aquino; Argentina. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia; ArgentinaFil: Benimeli, Claudia Susana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina. Universidad del Norte Santo Tomás de Aquino; ArgentinaElsevier2015-11info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/111607Sáez, Juliana María; Aparicio, Juan Daniel; Amoroso, Maria Julia del R.; Benimeli, Claudia Susana; Effect of the acclimation of a Streptomyces consortium on lindane biodegradation by free and immobilized cells; Elsevier; Process Biochemistry; 50; 11; 11-2015; 1923-19330032-9592CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/http://www.sciencedirect.com/science/article/pii/S1359511315300684info:eu-repo/semantics/altIdentifier/doi/10.1016/j.procbio.2015.08.014info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T10:09:55Zoai:ri.conicet.gov.ar:11336/111607instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 10:09:55.468CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Effect of the acclimation of a Streptomyces consortium on lindane biodegradation by free and immobilized cells
title Effect of the acclimation of a Streptomyces consortium on lindane biodegradation by free and immobilized cells
spellingShingle Effect of the acclimation of a Streptomyces consortium on lindane biodegradation by free and immobilized cells
Sáez, Juliana María
ACCLIMATION
BIODEGRADATION
CONSORTIUM
IMMBILIZATION
LINDANE
STREPTOMYCES
title_short Effect of the acclimation of a Streptomyces consortium on lindane biodegradation by free and immobilized cells
title_full Effect of the acclimation of a Streptomyces consortium on lindane biodegradation by free and immobilized cells
title_fullStr Effect of the acclimation of a Streptomyces consortium on lindane biodegradation by free and immobilized cells
title_full_unstemmed Effect of the acclimation of a Streptomyces consortium on lindane biodegradation by free and immobilized cells
title_sort Effect of the acclimation of a Streptomyces consortium on lindane biodegradation by free and immobilized cells
dc.creator.none.fl_str_mv Sáez, Juliana María
Aparicio, Juan Daniel
Amoroso, Maria Julia del R.
Benimeli, Claudia Susana
author Sáez, Juliana María
author_facet Sáez, Juliana María
Aparicio, Juan Daniel
Amoroso, Maria Julia del R.
Benimeli, Claudia Susana
author_role author
author2 Aparicio, Juan Daniel
Amoroso, Maria Julia del R.
Benimeli, Claudia Susana
author2_role author
author
author
dc.subject.none.fl_str_mv ACCLIMATION
BIODEGRADATION
CONSORTIUM
IMMBILIZATION
LINDANE
STREPTOMYCES
topic ACCLIMATION
BIODEGRADATION
CONSORTIUM
IMMBILIZATION
LINDANE
STREPTOMYCES
purl_subject.fl_str_mv https://purl.org/becyt/ford/2.8
https://purl.org/becyt/ford/2
dc.description.none.fl_txt_mv Lindane is an organochlorine pesticide widely used in agriculture, human health, and veterinary. It is a persistent organic pollutant, representing a serious risk since its residues are still in the environment. The aim of this work was to evaluate the influence of the acclimation of free and immobilized Streptomyces consortium on lindane degradation in liquid and slurry systems. The actinobacteria consortium demonstrated stability since the survival of all members was confirmed after the acclimation period. It reached higher biomass (0.56-0.65 g L-1) and lindane removal (40-97%) than the consortium without acclimation (0.37-0.44 g L-1; 33-87%) when it was cultured in liquid medium with 20 and 50 mg L-1 of lindane. In contrast, lindane removal achieved by acclimated and immobilized consortium in slurry system (50%) did not present significant difference respect to the consortium without acclimation. Moreover, lindane degradation was confirmed by identification of three metabolites in liquid medium, 1,2- and 1,4-dichlorobenzene, and γ-pentachlorocyclohexene; and by determination of chloride ions released in slurry system. Bioassays with Lactuca sativa demonstrated the effectiveness of the bioremediation by the acclimated consortium in both systems, since the seedlings showed an improvement in its biological parameters compared to abiotic controls, confirming a significant decrease in toxicity.
Fil: Sáez, Juliana María. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina
Fil: Aparicio, Juan Daniel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina
Fil: Amoroso, Maria Julia del R.. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina. Universidad del Norte Santo Tomás de Aquino; Argentina. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia; Argentina
Fil: Benimeli, Claudia Susana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina. Universidad del Norte Santo Tomás de Aquino; Argentina
description Lindane is an organochlorine pesticide widely used in agriculture, human health, and veterinary. It is a persistent organic pollutant, representing a serious risk since its residues are still in the environment. The aim of this work was to evaluate the influence of the acclimation of free and immobilized Streptomyces consortium on lindane degradation in liquid and slurry systems. The actinobacteria consortium demonstrated stability since the survival of all members was confirmed after the acclimation period. It reached higher biomass (0.56-0.65 g L-1) and lindane removal (40-97%) than the consortium without acclimation (0.37-0.44 g L-1; 33-87%) when it was cultured in liquid medium with 20 and 50 mg L-1 of lindane. In contrast, lindane removal achieved by acclimated and immobilized consortium in slurry system (50%) did not present significant difference respect to the consortium without acclimation. Moreover, lindane degradation was confirmed by identification of three metabolites in liquid medium, 1,2- and 1,4-dichlorobenzene, and γ-pentachlorocyclohexene; and by determination of chloride ions released in slurry system. Bioassays with Lactuca sativa demonstrated the effectiveness of the bioremediation by the acclimated consortium in both systems, since the seedlings showed an improvement in its biological parameters compared to abiotic controls, confirming a significant decrease in toxicity.
publishDate 2015
dc.date.none.fl_str_mv 2015-11
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/111607
Sáez, Juliana María; Aparicio, Juan Daniel; Amoroso, Maria Julia del R.; Benimeli, Claudia Susana; Effect of the acclimation of a Streptomyces consortium on lindane biodegradation by free and immobilized cells; Elsevier; Process Biochemistry; 50; 11; 11-2015; 1923-1933
0032-9592
CONICET Digital
CONICET
url http://hdl.handle.net/11336/111607
identifier_str_mv Sáez, Juliana María; Aparicio, Juan Daniel; Amoroso, Maria Julia del R.; Benimeli, Claudia Susana; Effect of the acclimation of a Streptomyces consortium on lindane biodegradation by free and immobilized cells; Elsevier; Process Biochemistry; 50; 11; 11-2015; 1923-1933
0032-9592
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/http://www.sciencedirect.com/science/article/pii/S1359511315300684
info:eu-repo/semantics/altIdentifier/doi/10.1016/j.procbio.2015.08.014
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.13397

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