Effects of bacterial cell-free supernatant on nutritional parameters of Apis Mellifera and their toxicity against Varroa Destructor

Autores
de Piano, Fiorella Giselle; Maggi, Matías Daniel; Meroi Arcerito, Facundo René; Audicio, Marcela C.; Eguaras, Martin Javier; Ruffinengo, Sergio Roberto
Año de publicación
2020
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Apis mellifera L. is an essential pollinator that is currently being affected by several stressors that disturb their ecological function and produce colony losses. Colonies are being seriously affected by the ectoparasitic mite Varroa destructor. The relationship between stressors and bee symbionts is being studied in order to enhance bee health. The goal of this study was to evaluate the effect of cell-free supernatants (CFSs) produced by Lactobacillus johnsonii AJ5, Enterococcus faecium SM21 and Bacillus subtilis subsp. subtilis Mori2 on A. mellifera nutritional parameters and their toxicity against V. destructor. Toxicity and survival bioassays were conducted on adult bees with different concentrations of CFSs. Nutritional parameters such as soluble proteins and fat bodies in abdomens were measured. Varroa destructor toxicity was analyzed by a contact exposure method and via bee hemolymph. At low concentrations, two of CFSs tends to enhance bee survival. Remarkably fat bodies maintained their levels with all CFS concentrations in the abdomens, and soluble protein increased at a high concentration of two CFSs. Toxicity against V. destructor was observed only via hemolymph, and results were in agreement with the treatment that produced an increase in bee proteins. Finally, CFS produced by L. johnsonii AJ5 could be a promising natural alternative for strengthening bee health.
Fil: de Piano, Fiorella Giselle. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales. Instituto de Investigaciones en Sanidad Producción y Ambiente. - Comisión de Investigaciones Científicas de la Provincia de Buenos Aires. Instituto de Investigaciones en Sanidad Producción y Ambiente; Argentina
Fil: Maggi, Matías Daniel. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales. Instituto de Investigaciones en Sanidad Producción y Ambiente. - Comisión de Investigaciones Científicas de la Provincia de Buenos Aires. Instituto de Investigaciones en Sanidad Producción y Ambiente; Argentina
Fil: Meroi Arcerito, Facundo René. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales. Instituto de Investigaciones en Sanidad Producción y Ambiente. - Comisión de Investigaciones Científicas de la Provincia de Buenos Aires. Instituto de Investigaciones en Sanidad Producción y Ambiente; Argentina
Fil: Audicio, Marcela C.. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Salta. Instituto de Investigaciones para la Industria Química. Universidad Nacional de Salta. Facultad de Ingeniería. Instituto de Investigaciones para la Industria Química; Argentina
Fil: Eguaras, Martin Javier. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales. Instituto de Investigaciones en Sanidad Producción y Ambiente. - Comisión de Investigaciones Científicas de la Provincia de Buenos Aires. Instituto de Investigaciones en Sanidad Producción y Ambiente; Argentina
Fil: Ruffinengo, Sergio Roberto. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales. Instituto de Investigaciones en Sanidad Producción y Ambiente. - Comisión de Investigaciones Científicas de la Provincia de Buenos Aires. Instituto de Investigaciones en Sanidad Producción y Ambiente; Argentina
Materia
APIS MELLIFERA
BACTERIAL METABOLITES
BENEFICIAL BACTERIA
NUTRITION
VARROA DESTRUCTOR
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/174974
Acceder
id CONICETDig_e8b3f063e64df1a2579e87868593ca5b
oai_identifier_str oai:ri.conicet.gov.ar:11336/174974
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Effects of bacterial cell-free supernatant on nutritional parameters of Apis Mellifera and their toxicity against Varroa Destructorde Piano, Fiorella GiselleMaggi, Matías DanielMeroi Arcerito, Facundo RenéAudicio, Marcela C.Eguaras, Martin JavierRuffinengo, Sergio RobertoAPIS MELLIFERABACTERIAL METABOLITESBENEFICIAL BACTERIANUTRITIONVARROA DESTRUCTORhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Apis mellifera L. is an essential pollinator that is currently being affected by several stressors that disturb their ecological function and produce colony losses. Colonies are being seriously affected by the ectoparasitic mite Varroa destructor. The relationship between stressors and bee symbionts is being studied in order to enhance bee health. The goal of this study was to evaluate the effect of cell-free supernatants (CFSs) produced by Lactobacillus johnsonii AJ5, Enterococcus faecium SM21 and Bacillus subtilis subsp. subtilis Mori2 on A. mellifera nutritional parameters and their toxicity against V. destructor. Toxicity and survival bioassays were conducted on adult bees with different concentrations of CFSs. Nutritional parameters such as soluble proteins and fat bodies in abdomens were measured. Varroa destructor toxicity was analyzed by a contact exposure method and via bee hemolymph. At low concentrations, two of CFSs tends to enhance bee survival. Remarkably fat bodies maintained their levels with all CFS concentrations in the abdomens, and soluble protein increased at a high concentration of two CFSs. Toxicity against V. destructor was observed only via hemolymph, and results were in agreement with the treatment that produced an increase in bee proteins. Finally, CFS produced by L. johnsonii AJ5 could be a promising natural alternative for strengthening bee health.Fil: de Piano, Fiorella Giselle. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales. Instituto de Investigaciones en Sanidad Producción y Ambiente. - Comisión de Investigaciones Científicas de la Provincia de Buenos Aires. Instituto de Investigaciones en Sanidad Producción y Ambiente; ArgentinaFil: Maggi, Matías Daniel. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales. Instituto de Investigaciones en Sanidad Producción y Ambiente. - Comisión de Investigaciones Científicas de la Provincia de Buenos Aires. Instituto de Investigaciones en Sanidad Producción y Ambiente; ArgentinaFil: Meroi Arcerito, Facundo René. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales. Instituto de Investigaciones en Sanidad Producción y Ambiente. - Comisión de Investigaciones Científicas de la Provincia de Buenos Aires. Instituto de Investigaciones en Sanidad Producción y Ambiente; ArgentinaFil: Audicio, Marcela C.. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Salta. Instituto de Investigaciones para la Industria Química. Universidad Nacional de Salta. Facultad de Ingeniería. Instituto de Investigaciones para la Industria Química; ArgentinaFil: Eguaras, Martin Javier. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales. Instituto de Investigaciones en Sanidad Producción y Ambiente. - Comisión de Investigaciones Científicas de la Provincia de Buenos Aires. Instituto de Investigaciones en Sanidad Producción y Ambiente; ArgentinaFil: Ruffinengo, Sergio Roberto. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales. Instituto de Investigaciones en Sanidad Producción y Ambiente. - Comisión de Investigaciones Científicas de la Provincia de Buenos Aires. Instituto de Investigaciones en Sanidad Producción y Ambiente; ArgentinaResearch Inst Pomology Floriculture2020-07info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/174974de Piano, Fiorella Giselle; Maggi, Matías Daniel; Meroi Arcerito, Facundo René; Audicio, Marcela C.; Eguaras, Martin Javier; et al.; Effects of bacterial cell-free supernatant on nutritional parameters of Apis Mellifera and their toxicity against Varroa Destructor; Research Inst Pomology Floriculture; Journal of Apicultural Science; 64; 1; 7-2020; 55-661643-4439CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://sciendo.com/article/10.2478/jas-2020-0009info:eu-repo/semantics/altIdentifier/doi/10.2478/jas-2020-0009info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T09:38:28Zoai:ri.conicet.gov.ar:11336/174974instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 09:38:28.646CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Effects of bacterial cell-free supernatant on nutritional parameters of Apis Mellifera and their toxicity against Varroa Destructor
title Effects of bacterial cell-free supernatant on nutritional parameters of Apis Mellifera and their toxicity against Varroa Destructor
spellingShingle Effects of bacterial cell-free supernatant on nutritional parameters of Apis Mellifera and their toxicity against Varroa Destructor
de Piano, Fiorella Giselle
APIS MELLIFERA
BACTERIAL METABOLITES
BENEFICIAL BACTERIA
NUTRITION
VARROA DESTRUCTOR
title_short Effects of bacterial cell-free supernatant on nutritional parameters of Apis Mellifera and their toxicity against Varroa Destructor
title_full Effects of bacterial cell-free supernatant on nutritional parameters of Apis Mellifera and their toxicity against Varroa Destructor
title_fullStr Effects of bacterial cell-free supernatant on nutritional parameters of Apis Mellifera and their toxicity against Varroa Destructor
title_full_unstemmed Effects of bacterial cell-free supernatant on nutritional parameters of Apis Mellifera and their toxicity against Varroa Destructor
title_sort Effects of bacterial cell-free supernatant on nutritional parameters of Apis Mellifera and their toxicity against Varroa Destructor
dc.creator.none.fl_str_mv de Piano, Fiorella Giselle
Maggi, Matías Daniel
Meroi Arcerito, Facundo René
Audicio, Marcela C.
Eguaras, Martin Javier
Ruffinengo, Sergio Roberto
author de Piano, Fiorella Giselle
author_facet de Piano, Fiorella Giselle
Maggi, Matías Daniel
Meroi Arcerito, Facundo René
Audicio, Marcela C.
Eguaras, Martin Javier
Ruffinengo, Sergio Roberto
author_role author
author2 Maggi, Matías Daniel
Meroi Arcerito, Facundo René
Audicio, Marcela C.
Eguaras, Martin Javier
Ruffinengo, Sergio Roberto
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv APIS MELLIFERA
BACTERIAL METABOLITES
BENEFICIAL BACTERIA
NUTRITION
VARROA DESTRUCTOR
topic APIS MELLIFERA
BACTERIAL METABOLITES
BENEFICIAL BACTERIA
NUTRITION
VARROA DESTRUCTOR
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Apis mellifera L. is an essential pollinator that is currently being affected by several stressors that disturb their ecological function and produce colony losses. Colonies are being seriously affected by the ectoparasitic mite Varroa destructor. The relationship between stressors and bee symbionts is being studied in order to enhance bee health. The goal of this study was to evaluate the effect of cell-free supernatants (CFSs) produced by Lactobacillus johnsonii AJ5, Enterococcus faecium SM21 and Bacillus subtilis subsp. subtilis Mori2 on A. mellifera nutritional parameters and their toxicity against V. destructor. Toxicity and survival bioassays were conducted on adult bees with different concentrations of CFSs. Nutritional parameters such as soluble proteins and fat bodies in abdomens were measured. Varroa destructor toxicity was analyzed by a contact exposure method and via bee hemolymph. At low concentrations, two of CFSs tends to enhance bee survival. Remarkably fat bodies maintained their levels with all CFS concentrations in the abdomens, and soluble protein increased at a high concentration of two CFSs. Toxicity against V. destructor was observed only via hemolymph, and results were in agreement with the treatment that produced an increase in bee proteins. Finally, CFS produced by L. johnsonii AJ5 could be a promising natural alternative for strengthening bee health.
Fil: de Piano, Fiorella Giselle. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales. Instituto de Investigaciones en Sanidad Producción y Ambiente. - Comisión de Investigaciones Científicas de la Provincia de Buenos Aires. Instituto de Investigaciones en Sanidad Producción y Ambiente; Argentina
Fil: Maggi, Matías Daniel. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales. Instituto de Investigaciones en Sanidad Producción y Ambiente. - Comisión de Investigaciones Científicas de la Provincia de Buenos Aires. Instituto de Investigaciones en Sanidad Producción y Ambiente; Argentina
Fil: Meroi Arcerito, Facundo René. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales. Instituto de Investigaciones en Sanidad Producción y Ambiente. - Comisión de Investigaciones Científicas de la Provincia de Buenos Aires. Instituto de Investigaciones en Sanidad Producción y Ambiente; Argentina
Fil: Audicio, Marcela C.. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Salta. Instituto de Investigaciones para la Industria Química. Universidad Nacional de Salta. Facultad de Ingeniería. Instituto de Investigaciones para la Industria Química; Argentina
Fil: Eguaras, Martin Javier. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales. Instituto de Investigaciones en Sanidad Producción y Ambiente. - Comisión de Investigaciones Científicas de la Provincia de Buenos Aires. Instituto de Investigaciones en Sanidad Producción y Ambiente; Argentina
Fil: Ruffinengo, Sergio Roberto. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales. Instituto de Investigaciones en Sanidad Producción y Ambiente. - Comisión de Investigaciones Científicas de la Provincia de Buenos Aires. Instituto de Investigaciones en Sanidad Producción y Ambiente; Argentina
description Apis mellifera L. is an essential pollinator that is currently being affected by several stressors that disturb their ecological function and produce colony losses. Colonies are being seriously affected by the ectoparasitic mite Varroa destructor. The relationship between stressors and bee symbionts is being studied in order to enhance bee health. The goal of this study was to evaluate the effect of cell-free supernatants (CFSs) produced by Lactobacillus johnsonii AJ5, Enterococcus faecium SM21 and Bacillus subtilis subsp. subtilis Mori2 on A. mellifera nutritional parameters and their toxicity against V. destructor. Toxicity and survival bioassays were conducted on adult bees with different concentrations of CFSs. Nutritional parameters such as soluble proteins and fat bodies in abdomens were measured. Varroa destructor toxicity was analyzed by a contact exposure method and via bee hemolymph. At low concentrations, two of CFSs tends to enhance bee survival. Remarkably fat bodies maintained their levels with all CFS concentrations in the abdomens, and soluble protein increased at a high concentration of two CFSs. Toxicity against V. destructor was observed only via hemolymph, and results were in agreement with the treatment that produced an increase in bee proteins. Finally, CFS produced by L. johnsonii AJ5 could be a promising natural alternative for strengthening bee health.
publishDate 2020
dc.date.none.fl_str_mv 2020-07
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/174974
de Piano, Fiorella Giselle; Maggi, Matías Daniel; Meroi Arcerito, Facundo René; Audicio, Marcela C.; Eguaras, Martin Javier; et al.; Effects of bacterial cell-free supernatant on nutritional parameters of Apis Mellifera and their toxicity against Varroa Destructor; Research Inst Pomology Floriculture; Journal of Apicultural Science; 64; 1; 7-2020; 55-66
1643-4439
CONICET Digital
CONICET
url http://hdl.handle.net/11336/174974
identifier_str_mv de Piano, Fiorella Giselle; Maggi, Matías Daniel; Meroi Arcerito, Facundo René; Audicio, Marcela C.; Eguaras, Martin Javier; et al.; Effects of bacterial cell-free supernatant on nutritional parameters of Apis Mellifera and their toxicity against Varroa Destructor; Research Inst Pomology Floriculture; Journal of Apicultural Science; 64; 1; 7-2020; 55-66
1643-4439
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://sciendo.com/article/10.2478/jas-2020-0009
info:eu-repo/semantics/altIdentifier/doi/10.2478/jas-2020-0009
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Research Inst Pomology Floriculture
publisher.none.fl_str_mv Research Inst Pomology Floriculture
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.070432

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