A standardized method to extract and store haemolymph from Apis mellifera and the ectoparasite Varroa destructor for protein analysis

Autores
Garrido, Paula Melisa; Martin, Mariana Laura; Negri, Pedro; Eguaras, Martin Javier
Año de publicación
2013
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The ectoparasitic mite Varroa destructor is a serious problem for beekeeping. It sucks haemolymph from different stages of the bees causing significant damage (see review by Rosenkranz et al., 2010). Haemolymph collection from honey bees is required to perform immunological, physiological, nutritional and other assays. In the literature there are many dissimilar methodologies to extract and store haemolymph. The electrophoretic profile and protein contents of V. destructor have been little studied, but it is of great interest to evaluate the changes experienced by this tissue in response to different treatments in the host and its parasite. The aim of this study is therefore to propose a standard procedure for haemolymph collection and storage, extending previous studies and sharing our working experience.
Fil: Garrido, Paula Melisa. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata; Argentina
Fil: Martin, Mariana Laura. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata; Argentina. Fundación para Investigaciones Biológicas Aplicadas; Argentina
Fil: Negri, Pedro. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata; Argentina
Fil: Eguaras, Martin Javier. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata; Argentina. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales; Argentina
Materia
APIS MELLIFERA
HAEMOLYMPH
STANDARDIZED METHOD
VARROA DESTRUCTOR
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/25068
Acceder
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network_name_str CONICET Digital (CONICET)
spelling A standardized method to extract and store haemolymph from Apis mellifera and the ectoparasite Varroa destructor for protein analysisGarrido, Paula MelisaMartin, Mariana LauraNegri, PedroEguaras, Martin JavierAPIS MELLIFERAHAEMOLYMPHSTANDARDIZED METHODVARROA DESTRUCTORhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1The ectoparasitic mite Varroa destructor is a serious problem for beekeeping. It sucks haemolymph from different stages of the bees causing significant damage (see review by Rosenkranz et al., 2010). Haemolymph collection from honey bees is required to perform immunological, physiological, nutritional and other assays. In the literature there are many dissimilar methodologies to extract and store haemolymph. The electrophoretic profile and protein contents of V. destructor have been little studied, but it is of great interest to evaluate the changes experienced by this tissue in response to different treatments in the host and its parasite. The aim of this study is therefore to propose a standard procedure for haemolymph collection and storage, extending previous studies and sharing our working experience.Fil: Garrido, Paula Melisa. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata; ArgentinaFil: Martin, Mariana Laura. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata; Argentina. Fundación para Investigaciones Biológicas Aplicadas; ArgentinaFil: Negri, Pedro. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata; ArgentinaFil: Eguaras, Martin Javier. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata; Argentina. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales; ArgentinaInternational Bee Research Association2013-04info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/25068Garrido, Paula Melisa; Martin, Mariana Laura; Negri, Pedro; Eguaras, Martin Javier; A standardized method to extract and store haemolymph from Apis mellifera and the ectoparasite Varroa destructor for protein analysis; International Bee Research Association; Journal Of Apicultural Research; 52; 2; 4-2013; 67-680021-8839CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.3896/IBRA.1.52.2.13info:eu-repo/semantics/altIdentifier/url/http://www.tandfonline.com/doi/abs/10.3896/IBRA.1.52.2.13info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-10T13:06:46Zoai:ri.conicet.gov.ar:11336/25068instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-10 13:06:47.091CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv A standardized method to extract and store haemolymph from Apis mellifera and the ectoparasite Varroa destructor for protein analysis
title A standardized method to extract and store haemolymph from Apis mellifera and the ectoparasite Varroa destructor for protein analysis
spellingShingle A standardized method to extract and store haemolymph from Apis mellifera and the ectoparasite Varroa destructor for protein analysis
Garrido, Paula Melisa
APIS MELLIFERA
HAEMOLYMPH
STANDARDIZED METHOD
VARROA DESTRUCTOR
title_short A standardized method to extract and store haemolymph from Apis mellifera and the ectoparasite Varroa destructor for protein analysis
title_full A standardized method to extract and store haemolymph from Apis mellifera and the ectoparasite Varroa destructor for protein analysis
title_fullStr A standardized method to extract and store haemolymph from Apis mellifera and the ectoparasite Varroa destructor for protein analysis
title_full_unstemmed A standardized method to extract and store haemolymph from Apis mellifera and the ectoparasite Varroa destructor for protein analysis
title_sort A standardized method to extract and store haemolymph from Apis mellifera and the ectoparasite Varroa destructor for protein analysis
dc.creator.none.fl_str_mv Garrido, Paula Melisa
Martin, Mariana Laura
Negri, Pedro
Eguaras, Martin Javier
author Garrido, Paula Melisa
author_facet Garrido, Paula Melisa
Martin, Mariana Laura
Negri, Pedro
Eguaras, Martin Javier
author_role author
author2 Martin, Mariana Laura
Negri, Pedro
Eguaras, Martin Javier
author2_role author
author
author
dc.subject.none.fl_str_mv APIS MELLIFERA
HAEMOLYMPH
STANDARDIZED METHOD
VARROA DESTRUCTOR
topic APIS MELLIFERA
HAEMOLYMPH
STANDARDIZED METHOD
VARROA DESTRUCTOR
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv The ectoparasitic mite Varroa destructor is a serious problem for beekeeping. It sucks haemolymph from different stages of the bees causing significant damage (see review by Rosenkranz et al., 2010). Haemolymph collection from honey bees is required to perform immunological, physiological, nutritional and other assays. In the literature there are many dissimilar methodologies to extract and store haemolymph. The electrophoretic profile and protein contents of V. destructor have been little studied, but it is of great interest to evaluate the changes experienced by this tissue in response to different treatments in the host and its parasite. The aim of this study is therefore to propose a standard procedure for haemolymph collection and storage, extending previous studies and sharing our working experience.
Fil: Garrido, Paula Melisa. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata; Argentina
Fil: Martin, Mariana Laura. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata; Argentina. Fundación para Investigaciones Biológicas Aplicadas; Argentina
Fil: Negri, Pedro. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata; Argentina
Fil: Eguaras, Martin Javier. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata; Argentina. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales; Argentina
description The ectoparasitic mite Varroa destructor is a serious problem for beekeeping. It sucks haemolymph from different stages of the bees causing significant damage (see review by Rosenkranz et al., 2010). Haemolymph collection from honey bees is required to perform immunological, physiological, nutritional and other assays. In the literature there are many dissimilar methodologies to extract and store haemolymph. The electrophoretic profile and protein contents of V. destructor have been little studied, but it is of great interest to evaluate the changes experienced by this tissue in response to different treatments in the host and its parasite. The aim of this study is therefore to propose a standard procedure for haemolymph collection and storage, extending previous studies and sharing our working experience.
publishDate 2013
dc.date.none.fl_str_mv 2013-04
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/25068
Garrido, Paula Melisa; Martin, Mariana Laura; Negri, Pedro; Eguaras, Martin Javier; A standardized method to extract and store haemolymph from Apis mellifera and the ectoparasite Varroa destructor for protein analysis; International Bee Research Association; Journal Of Apicultural Research; 52; 2; 4-2013; 67-68
0021-8839
CONICET Digital
CONICET
url http://hdl.handle.net/11336/25068
identifier_str_mv Garrido, Paula Melisa; Martin, Mariana Laura; Negri, Pedro; Eguaras, Martin Javier; A standardized method to extract and store haemolymph from Apis mellifera and the ectoparasite Varroa destructor for protein analysis; International Bee Research Association; Journal Of Apicultural Research; 52; 2; 4-2013; 67-68
0021-8839
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.3896/IBRA.1.52.2.13
info:eu-repo/semantics/altIdentifier/url/http://www.tandfonline.com/doi/abs/10.3896/IBRA.1.52.2.13
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv International Bee Research Association
publisher.none.fl_str_mv International Bee Research Association
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1842980289497792512
score 12.993085

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