Extracellular Sunflower Proteins: Evidence on Non-classical Secretion of a Jacalin-Related Lectin

Autores
Pinedo, Marcela Lilian; Regente, Mariana Clelia; Elizalde, Maria Mercedes; Quiroga, Ivana Yoseli; Pagnussat, Luciana Anabella; Jorrín Novo, Jesús; Maldonado, Ana; de la Canal, Laura
Año de publicación
2012
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Extracellular proteins from sunflower seedlings were analyzed by electrophoresis followed by peptide mass fingerprinting. Tentative identification revealed novel proteins for this crop. A significant number of those proteins were not expected to be extracellular because they lacked the typical signal peptide responsible for secretion. In silico analysis showed that some members of this group presented the characteristic disordered structures of certain non-classical and leaderless mammalian secretory proteins. Among these proteins, a putative jacalin-related lectin (Helja) with a mannose binding domain was further isolated from extracellular fluids by mannose-affinity chromatography, thus validating its identification. Besides, immunolocalization assays confirmed its extracellular location. These results showed that a lectin, not predicted to be secreted in strict requirement of the N-terminal signal peptide, occurs in a sunflower extracellular compartment. The implications of this finding are discussed.
Fil: Pinedo, Marcela Lilian. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones Biológicas. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales. Instituto de Investigaciones Biológicas; Argentina
Fil: Regente, Mariana Clelia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones Biológicas. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales. Instituto de Investigaciones Biológicas; Argentina
Fil: Elizalde, Maria Mercedes. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones Biológicas. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales. Instituto de Investigaciones Biológicas; Argentina
Fil: Quiroga, Ivana Yoseli. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones Biológicas. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales. Instituto de Investigaciones Biológicas; Argentina
Fil: Pagnussat, Luciana Anabella. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones Biológicas. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales. Instituto de Investigaciones Biológicas; Argentina
Fil: Jorrín Novo, Jesús. Universidad de Córdoba; España
Fil: Maldonado, Ana. Universidad de Córdoba; España
Fil: de la Canal, Laura. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones Biológicas. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales. Instituto de Investigaciones Biológicas; Argentina
Materia
AGGLUTININ
HELIANTHUS ANNUS
JACALIN
PEPTIDE MASS FINGERPRINTING
SECRETOME P
SIGNAL SEQUENCE
UNCONVENTIONAL SECRETION
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/157660
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/157660
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Extracellular Sunflower Proteins: Evidence on Non-classical Secretion of a Jacalin-Related LectinPinedo, Marcela LilianRegente, Mariana CleliaElizalde, Maria MercedesQuiroga, Ivana YoseliPagnussat, Luciana AnabellaJorrín Novo, JesúsMaldonado, Anade la Canal, LauraAGGLUTININHELIANTHUS ANNUSJACALINPEPTIDE MASS FINGERPRINTINGSECRETOME PSIGNAL SEQUENCEUNCONVENTIONAL SECRETIONhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Extracellular proteins from sunflower seedlings were analyzed by electrophoresis followed by peptide mass fingerprinting. Tentative identification revealed novel proteins for this crop. A significant number of those proteins were not expected to be extracellular because they lacked the typical signal peptide responsible for secretion. In silico analysis showed that some members of this group presented the characteristic disordered structures of certain non-classical and leaderless mammalian secretory proteins. Among these proteins, a putative jacalin-related lectin (Helja) with a mannose binding domain was further isolated from extracellular fluids by mannose-affinity chromatography, thus validating its identification. Besides, immunolocalization assays confirmed its extracellular location. These results showed that a lectin, not predicted to be secreted in strict requirement of the N-terminal signal peptide, occurs in a sunflower extracellular compartment. The implications of this finding are discussed.Fil: Pinedo, Marcela Lilian. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones Biológicas. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales. Instituto de Investigaciones Biológicas; ArgentinaFil: Regente, Mariana Clelia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones Biológicas. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales. Instituto de Investigaciones Biológicas; ArgentinaFil: Elizalde, Maria Mercedes. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones Biológicas. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales. Instituto de Investigaciones Biológicas; ArgentinaFil: Quiroga, Ivana Yoseli. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones Biológicas. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales. Instituto de Investigaciones Biológicas; ArgentinaFil: Pagnussat, Luciana Anabella. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones Biológicas. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales. Instituto de Investigaciones Biológicas; ArgentinaFil: Jorrín Novo, Jesús. Universidad de Córdoba; EspañaFil: Maldonado, Ana. Universidad de Córdoba; EspañaFil: de la Canal, Laura. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones Biológicas. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales. Instituto de Investigaciones Biológicas; ArgentinaBentham Science Publishers2012-03info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/157660Pinedo, Marcela Lilian; Regente, Mariana Clelia; Elizalde, Maria Mercedes; Quiroga, Ivana Yoseli; Pagnussat, Luciana Anabella; et al.; Extracellular Sunflower Proteins: Evidence on Non-classical Secretion of a Jacalin-Related Lectin; Bentham Science Publishers; Protein and Peptide Letters; 19; 3; 3-2012; 270-2760929-86651875-5305CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/http://www.eurekaselect.com/article/34616info:eu-repo/semantics/altIdentifier/doi/10.2174/092986612799363163info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-10T13:01:26Zoai:ri.conicet.gov.ar:11336/157660instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-10 13:01:27.188CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Extracellular Sunflower Proteins: Evidence on Non-classical Secretion of a Jacalin-Related Lectin
title Extracellular Sunflower Proteins: Evidence on Non-classical Secretion of a Jacalin-Related Lectin
spellingShingle Extracellular Sunflower Proteins: Evidence on Non-classical Secretion of a Jacalin-Related Lectin
Pinedo, Marcela Lilian
AGGLUTININ
HELIANTHUS ANNUS
JACALIN
PEPTIDE MASS FINGERPRINTING
SECRETOME P
SIGNAL SEQUENCE
UNCONVENTIONAL SECRETION
title_short Extracellular Sunflower Proteins: Evidence on Non-classical Secretion of a Jacalin-Related Lectin
title_full Extracellular Sunflower Proteins: Evidence on Non-classical Secretion of a Jacalin-Related Lectin
title_fullStr Extracellular Sunflower Proteins: Evidence on Non-classical Secretion of a Jacalin-Related Lectin
title_full_unstemmed Extracellular Sunflower Proteins: Evidence on Non-classical Secretion of a Jacalin-Related Lectin
title_sort Extracellular Sunflower Proteins: Evidence on Non-classical Secretion of a Jacalin-Related Lectin
dc.creator.none.fl_str_mv Pinedo, Marcela Lilian
Regente, Mariana Clelia
Elizalde, Maria Mercedes
Quiroga, Ivana Yoseli
Pagnussat, Luciana Anabella
Jorrín Novo, Jesús
Maldonado, Ana
de la Canal, Laura
author Pinedo, Marcela Lilian
author_facet Pinedo, Marcela Lilian
Regente, Mariana Clelia
Elizalde, Maria Mercedes
Quiroga, Ivana Yoseli
Pagnussat, Luciana Anabella
Jorrín Novo, Jesús
Maldonado, Ana
de la Canal, Laura
author_role author
author2 Regente, Mariana Clelia
Elizalde, Maria Mercedes
Quiroga, Ivana Yoseli
Pagnussat, Luciana Anabella
Jorrín Novo, Jesús
Maldonado, Ana
de la Canal, Laura
author2_role author
author
author
author
author
author
author
dc.subject.none.fl_str_mv AGGLUTININ
HELIANTHUS ANNUS
JACALIN
PEPTIDE MASS FINGERPRINTING
SECRETOME P
SIGNAL SEQUENCE
UNCONVENTIONAL SECRETION
topic AGGLUTININ
HELIANTHUS ANNUS
JACALIN
PEPTIDE MASS FINGERPRINTING
SECRETOME P
SIGNAL SEQUENCE
UNCONVENTIONAL SECRETION
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Extracellular proteins from sunflower seedlings were analyzed by electrophoresis followed by peptide mass fingerprinting. Tentative identification revealed novel proteins for this crop. A significant number of those proteins were not expected to be extracellular because they lacked the typical signal peptide responsible for secretion. In silico analysis showed that some members of this group presented the characteristic disordered structures of certain non-classical and leaderless mammalian secretory proteins. Among these proteins, a putative jacalin-related lectin (Helja) with a mannose binding domain was further isolated from extracellular fluids by mannose-affinity chromatography, thus validating its identification. Besides, immunolocalization assays confirmed its extracellular location. These results showed that a lectin, not predicted to be secreted in strict requirement of the N-terminal signal peptide, occurs in a sunflower extracellular compartment. The implications of this finding are discussed.
Fil: Pinedo, Marcela Lilian. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones Biológicas. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales. Instituto de Investigaciones Biológicas; Argentina
Fil: Regente, Mariana Clelia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones Biológicas. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales. Instituto de Investigaciones Biológicas; Argentina
Fil: Elizalde, Maria Mercedes. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones Biológicas. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales. Instituto de Investigaciones Biológicas; Argentina
Fil: Quiroga, Ivana Yoseli. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones Biológicas. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales. Instituto de Investigaciones Biológicas; Argentina
Fil: Pagnussat, Luciana Anabella. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones Biológicas. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales. Instituto de Investigaciones Biológicas; Argentina
Fil: Jorrín Novo, Jesús. Universidad de Córdoba; España
Fil: Maldonado, Ana. Universidad de Córdoba; España
Fil: de la Canal, Laura. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones Biológicas. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales. Instituto de Investigaciones Biológicas; Argentina
description Extracellular proteins from sunflower seedlings were analyzed by electrophoresis followed by peptide mass fingerprinting. Tentative identification revealed novel proteins for this crop. A significant number of those proteins were not expected to be extracellular because they lacked the typical signal peptide responsible for secretion. In silico analysis showed that some members of this group presented the characteristic disordered structures of certain non-classical and leaderless mammalian secretory proteins. Among these proteins, a putative jacalin-related lectin (Helja) with a mannose binding domain was further isolated from extracellular fluids by mannose-affinity chromatography, thus validating its identification. Besides, immunolocalization assays confirmed its extracellular location. These results showed that a lectin, not predicted to be secreted in strict requirement of the N-terminal signal peptide, occurs in a sunflower extracellular compartment. The implications of this finding are discussed.
publishDate 2012
dc.date.none.fl_str_mv 2012-03
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/157660
Pinedo, Marcela Lilian; Regente, Mariana Clelia; Elizalde, Maria Mercedes; Quiroga, Ivana Yoseli; Pagnussat, Luciana Anabella; et al.; Extracellular Sunflower Proteins: Evidence on Non-classical Secretion of a Jacalin-Related Lectin; Bentham Science Publishers; Protein and Peptide Letters; 19; 3; 3-2012; 270-276
0929-8665
1875-5305
CONICET Digital
CONICET
url http://hdl.handle.net/11336/157660
identifier_str_mv Pinedo, Marcela Lilian; Regente, Mariana Clelia; Elizalde, Maria Mercedes; Quiroga, Ivana Yoseli; Pagnussat, Luciana Anabella; et al.; Extracellular Sunflower Proteins: Evidence on Non-classical Secretion of a Jacalin-Related Lectin; Bentham Science Publishers; Protein and Peptide Letters; 19; 3; 3-2012; 270-276
0929-8665
1875-5305
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/http://www.eurekaselect.com/article/34616
info:eu-repo/semantics/altIdentifier/doi/10.2174/092986612799363163
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Bentham Science Publishers
publisher.none.fl_str_mv Bentham Science Publishers
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 12.993085

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