Insecticidal Activity of Microencapsulated Vip3Ag4 protein in Bacillus megaterium

Autores
Palma, Leopoldo; Ruiz de Escudero, Iñigo; Mañeru Oria , Francisco; Berry, Colin; Caballero, Primitivo
Año de publicación
2023
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Bacillus thuringiensis (Bt) produces, during its vegetative growth, some insecticidal proteins that are secreted and diluted into the culture medium. These proteins are commonly known as vegetative insecticidal proteins (Vips), and include binary Vpb/Vpa proteins (formerly known as Vip1/Vip2) with coleopteran activity; Vip (formerly Vip3) with activity against lepidopterans; and Vpb4 proteins (formerly Vip4), also with coleopteran activity. Vip proteins are highly toxic to different species of lepidopteran pests; however, the difficulty in producing them in a concentrated form has not allowed their development as formulated biopesticides, and they are relegated to only being produced in transgenic crops. In this work, we demonstrated that the gene encoding the Vip protein Vip3Ag4 could be successfully expressed in an asporogenic strain of Bacillus megaterium using (D)-xylose as a low-cost inductor. Under certain conditions (37 °C and induction with 0.5% w/v xylose), active Vip3Ag4 protein was primarily produced in soluble form, remaining encapsulated within the cell wall of B. megaterium. After treatment with lugol (1% for 4 h), induced cells were completely killed (fixed) but maintained the functional Vip3Ag4 protein, which resulted in above 95% mortality against first-instar larvae of Chrysodeixis chalcites, Helicoverpa armigera, Spodoptera exigua, S. frugiperda, S. littoralis and Trichoplusia ni. The fact that the recombinant Vip3Ag4 protein was successfully produced in a soluble and an active form in this bacterium (with a low-cost inductor) suggests that B. megaterium is one of the hosts of choice for the production of sprayable formulations in “killed-microbial pesticides”, based on Vip3 proteins from Bt.
Fil: Palma, Leopoldo. Universidad de Valencia; España. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto Multidisciplinario de Investigación y Transferencia Agroalimentaria y Biotecnológica - Universidad Nacional de Villa María. Instituto Multidisciplinario de Investigación y Transferencia Agroalimentaria y Biotecnológica; Argentina
Fil: Ruiz de Escudero, Iñigo. Universidad de Navarra; España
Fil: Mañeru Oria , Francisco. Universidad de Navarra; España
Fil: Berry, Colin. Cardiff University; Reino Unido
Fil: Caballero, Primitivo. Universidad de Navarra; España
Materia
BACILLUS THURINGIENSI
VIP3 PROTEINS
INSECTICIDAL PROTEINS
BIOLOGICAL CONTROL
INSECT PESTS
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/226876

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network_name_str CONICET Digital (CONICET)
spelling Insecticidal Activity of Microencapsulated Vip3Ag4 protein in Bacillus megateriumPalma, LeopoldoRuiz de Escudero, IñigoMañeru Oria , FranciscoBerry, ColinCaballero, PrimitivoBACILLUS THURINGIENSIVIP3 PROTEINSINSECTICIDAL PROTEINSBIOLOGICAL CONTROLINSECT PESTShttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Bacillus thuringiensis (Bt) produces, during its vegetative growth, some insecticidal proteins that are secreted and diluted into the culture medium. These proteins are commonly known as vegetative insecticidal proteins (Vips), and include binary Vpb/Vpa proteins (formerly known as Vip1/Vip2) with coleopteran activity; Vip (formerly Vip3) with activity against lepidopterans; and Vpb4 proteins (formerly Vip4), also with coleopteran activity. Vip proteins are highly toxic to different species of lepidopteran pests; however, the difficulty in producing them in a concentrated form has not allowed their development as formulated biopesticides, and they are relegated to only being produced in transgenic crops. In this work, we demonstrated that the gene encoding the Vip protein Vip3Ag4 could be successfully expressed in an asporogenic strain of Bacillus megaterium using (D)-xylose as a low-cost inductor. Under certain conditions (37 °C and induction with 0.5% w/v xylose), active Vip3Ag4 protein was primarily produced in soluble form, remaining encapsulated within the cell wall of B. megaterium. After treatment with lugol (1% for 4 h), induced cells were completely killed (fixed) but maintained the functional Vip3Ag4 protein, which resulted in above 95% mortality against first-instar larvae of Chrysodeixis chalcites, Helicoverpa armigera, Spodoptera exigua, S. frugiperda, S. littoralis and Trichoplusia ni. The fact that the recombinant Vip3Ag4 protein was successfully produced in a soluble and an active form in this bacterium (with a low-cost inductor) suggests that B. megaterium is one of the hosts of choice for the production of sprayable formulations in “killed-microbial pesticides”, based on Vip3 proteins from Bt.Fil: Palma, Leopoldo. Universidad de Valencia; España. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto Multidisciplinario de Investigación y Transferencia Agroalimentaria y Biotecnológica - Universidad Nacional de Villa María. Instituto Multidisciplinario de Investigación y Transferencia Agroalimentaria y Biotecnológica; ArgentinaFil: Ruiz de Escudero, Iñigo. Universidad de Navarra; EspañaFil: Mañeru Oria , Francisco. Universidad de Navarra; EspañaFil: Berry, Colin. Cardiff University; Reino UnidoFil: Caballero, Primitivo. Universidad de Navarra; EspañaMDPI2023-12info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/226876Palma, Leopoldo; Ruiz de Escudero, Iñigo; Mañeru Oria , Francisco; Berry, Colin; Caballero, Primitivo; Insecticidal Activity of Microencapsulated Vip3Ag4 protein in Bacillus megaterium; MDPI; Biology and Life Sciences Forum; 24; 12-2023; 1-82673-9976CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.mdpi.com/2673-9976/24/1/9info:eu-repo/semantics/altIdentifier/doi/10.3390/IECT2023-14822info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T09:58:02Zoai:ri.conicet.gov.ar:11336/226876instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 09:58:02.284CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Insecticidal Activity of Microencapsulated Vip3Ag4 protein in Bacillus megaterium
title Insecticidal Activity of Microencapsulated Vip3Ag4 protein in Bacillus megaterium
spellingShingle Insecticidal Activity of Microencapsulated Vip3Ag4 protein in Bacillus megaterium
Palma, Leopoldo
BACILLUS THURINGIENSI
VIP3 PROTEINS
INSECTICIDAL PROTEINS
BIOLOGICAL CONTROL
INSECT PESTS
title_short Insecticidal Activity of Microencapsulated Vip3Ag4 protein in Bacillus megaterium
title_full Insecticidal Activity of Microencapsulated Vip3Ag4 protein in Bacillus megaterium
title_fullStr Insecticidal Activity of Microencapsulated Vip3Ag4 protein in Bacillus megaterium
title_full_unstemmed Insecticidal Activity of Microencapsulated Vip3Ag4 protein in Bacillus megaterium
title_sort Insecticidal Activity of Microencapsulated Vip3Ag4 protein in Bacillus megaterium
dc.creator.none.fl_str_mv Palma, Leopoldo
Ruiz de Escudero, Iñigo
Mañeru Oria , Francisco
Berry, Colin
Caballero, Primitivo
author Palma, Leopoldo
author_facet Palma, Leopoldo
Ruiz de Escudero, Iñigo
Mañeru Oria , Francisco
Berry, Colin
Caballero, Primitivo
author_role author
author2 Ruiz de Escudero, Iñigo
Mañeru Oria , Francisco
Berry, Colin
Caballero, Primitivo
author2_role author
author
author
author
dc.subject.none.fl_str_mv BACILLUS THURINGIENSI
VIP3 PROTEINS
INSECTICIDAL PROTEINS
BIOLOGICAL CONTROL
INSECT PESTS
topic BACILLUS THURINGIENSI
VIP3 PROTEINS
INSECTICIDAL PROTEINS
BIOLOGICAL CONTROL
INSECT PESTS
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Bacillus thuringiensis (Bt) produces, during its vegetative growth, some insecticidal proteins that are secreted and diluted into the culture medium. These proteins are commonly known as vegetative insecticidal proteins (Vips), and include binary Vpb/Vpa proteins (formerly known as Vip1/Vip2) with coleopteran activity; Vip (formerly Vip3) with activity against lepidopterans; and Vpb4 proteins (formerly Vip4), also with coleopteran activity. Vip proteins are highly toxic to different species of lepidopteran pests; however, the difficulty in producing them in a concentrated form has not allowed their development as formulated biopesticides, and they are relegated to only being produced in transgenic crops. In this work, we demonstrated that the gene encoding the Vip protein Vip3Ag4 could be successfully expressed in an asporogenic strain of Bacillus megaterium using (D)-xylose as a low-cost inductor. Under certain conditions (37 °C and induction with 0.5% w/v xylose), active Vip3Ag4 protein was primarily produced in soluble form, remaining encapsulated within the cell wall of B. megaterium. After treatment with lugol (1% for 4 h), induced cells were completely killed (fixed) but maintained the functional Vip3Ag4 protein, which resulted in above 95% mortality against first-instar larvae of Chrysodeixis chalcites, Helicoverpa armigera, Spodoptera exigua, S. frugiperda, S. littoralis and Trichoplusia ni. The fact that the recombinant Vip3Ag4 protein was successfully produced in a soluble and an active form in this bacterium (with a low-cost inductor) suggests that B. megaterium is one of the hosts of choice for the production of sprayable formulations in “killed-microbial pesticides”, based on Vip3 proteins from Bt.
Fil: Palma, Leopoldo. Universidad de Valencia; España. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto Multidisciplinario de Investigación y Transferencia Agroalimentaria y Biotecnológica - Universidad Nacional de Villa María. Instituto Multidisciplinario de Investigación y Transferencia Agroalimentaria y Biotecnológica; Argentina
Fil: Ruiz de Escudero, Iñigo. Universidad de Navarra; España
Fil: Mañeru Oria , Francisco. Universidad de Navarra; España
Fil: Berry, Colin. Cardiff University; Reino Unido
Fil: Caballero, Primitivo. Universidad de Navarra; España
description Bacillus thuringiensis (Bt) produces, during its vegetative growth, some insecticidal proteins that are secreted and diluted into the culture medium. These proteins are commonly known as vegetative insecticidal proteins (Vips), and include binary Vpb/Vpa proteins (formerly known as Vip1/Vip2) with coleopteran activity; Vip (formerly Vip3) with activity against lepidopterans; and Vpb4 proteins (formerly Vip4), also with coleopteran activity. Vip proteins are highly toxic to different species of lepidopteran pests; however, the difficulty in producing them in a concentrated form has not allowed their development as formulated biopesticides, and they are relegated to only being produced in transgenic crops. In this work, we demonstrated that the gene encoding the Vip protein Vip3Ag4 could be successfully expressed in an asporogenic strain of Bacillus megaterium using (D)-xylose as a low-cost inductor. Under certain conditions (37 °C and induction with 0.5% w/v xylose), active Vip3Ag4 protein was primarily produced in soluble form, remaining encapsulated within the cell wall of B. megaterium. After treatment with lugol (1% for 4 h), induced cells were completely killed (fixed) but maintained the functional Vip3Ag4 protein, which resulted in above 95% mortality against first-instar larvae of Chrysodeixis chalcites, Helicoverpa armigera, Spodoptera exigua, S. frugiperda, S. littoralis and Trichoplusia ni. The fact that the recombinant Vip3Ag4 protein was successfully produced in a soluble and an active form in this bacterium (with a low-cost inductor) suggests that B. megaterium is one of the hosts of choice for the production of sprayable formulations in “killed-microbial pesticides”, based on Vip3 proteins from Bt.
publishDate 2023
dc.date.none.fl_str_mv 2023-12
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/226876
Palma, Leopoldo; Ruiz de Escudero, Iñigo; Mañeru Oria , Francisco; Berry, Colin; Caballero, Primitivo; Insecticidal Activity of Microencapsulated Vip3Ag4 protein in Bacillus megaterium; MDPI; Biology and Life Sciences Forum; 24; 12-2023; 1-8
2673-9976
CONICET Digital
CONICET
url http://hdl.handle.net/11336/226876
identifier_str_mv Palma, Leopoldo; Ruiz de Escudero, Iñigo; Mañeru Oria , Francisco; Berry, Colin; Caballero, Primitivo; Insecticidal Activity of Microencapsulated Vip3Ag4 protein in Bacillus megaterium; MDPI; Biology and Life Sciences Forum; 24; 12-2023; 1-8
2673-9976
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://www.mdpi.com/2673-9976/24/1/9
info:eu-repo/semantics/altIdentifier/doi/10.3390/IECT2023-14822
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv MDPI
publisher.none.fl_str_mv MDPI
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
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instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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