Bacillus thuringiensis Bt_UNVM-84, a Novel Strain Showing Insecticidal Activity against Anthonomus grandis Boheman (Coleoptera: Curculionidae)

Autores
Sauka, Diego Hernan; Peralta, Cecilia; Pérez, Melisa Paula; Molla, Antonella; Fernandez-Göbel, Tadeo; Ocampo, Federico; Palma, Leopoldo
Año de publicación
2024
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Bacillus thuringiensis is a Gram-positive bacterium known for its insecticidal proteins effective against various insect pests. However, limited strains and proteins target coleopteran pests like Anthonomous grandis Boheman, causing substantial economic losses in the cotton industry. This study focuses on characterizing a Bacillus sp. strain, isolated from Oncativo (Argentina), which exhibits ovoid to amorphous parasporal crystals and was designated Bt_UNVM-84. Its genome encodes genes for the production of two pairs of binary Vpb1/Vpa2 proteins and three Cry-like proteins showing similarity with different Cry8 proteins. Interestingly, this gene content was found to be conserved in a previously characterized Argentine isolate of B. thuringiensis designated INTA Fr7-4. SDS-PAGE analysis revealed a major band of 130 kDa that is proteolytically processed to an approximately 66-kDa protein fragment by trypsin. Bioassays performed with spore-crystal mixtures demonstrated an interesting insecticidal activity against the cotton boll weevil A. grandis neonate larvae, resulting in 91% mortality. Strain Bt_UNVM-84 is, therefore, an interesting candidate for the efficient biological control of this species, causing significant economic losses in the cotton industry in the Americas.
Instituto de Microbiología y Zoología Agrícola (IMYZA)
Fil: Sauka, Diego Herman. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Microbiología y Zoología Agrícola; Argentina
Fil: Sauka, Diego Herman. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Peralta, Cecilia. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Peralta, Cecilia. Universidad Nacional de Villa María. Instituto Multidisciplinario de Investigación y Transferencia Agroalimentaria y Biotecnológica; Argentina
Fil: Peralta, Cecilia. Universitat de València. Instituto BIOTECMED. Departamento de Genética. Laboratorio de Control Biotecnológico de Plagas; España
Fil: Pérez, Melisa Paula. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Microbiología y Zoología Agrícola; Argentina
Fil: Molla, Antonella. Universidad Nacional de Villa María. Instituto Multidisciplinario de Investigación y Transferencia Agroalimentaria y Biotecnológica; Argentina
Fil: Fernandez-Göbel, Tadeo. Elytron Biotech S.A.; Argentina
Fil: Ocampo, Federico. Elytron Biotech S.A.; Argentina
Fil: Palma, Leopoldo. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Palma, Leopoldo. Universidad Nacional de Villa María. Instituto Multidisciplinario de Investigación y Transferencia Agroalimentaria y Biotecnológica; Argentina
Fil: Palma, Leopoldo. Universitat de València. Instituto BIOTECMED. Departamento de Genética. Laboratorio de Control Biotecnológico de Plagas; España
Fuente
Toxins 16 (1) : 4 (January 2024)
Materia
Bacillus thuringiensis
Control Biológico
Plagas
Biological Control
Pests
Insecticidal Proteins
Vpb1/Vpa2 Proteins
Cry8 Proteins
Bioinsecticides
Nivel de accesibilidad
acceso abierto
Condiciones de uso
http://creativecommons.org/licenses/by-nc-sa/4.0/
Repositorio
INTA Digital (INTA)
Institución
Instituto Nacional de Tecnología Agropecuaria
OAI Identificador
oai:localhost:20.500.12123/16588
Acceder
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oai_identifier_str oai:localhost:20.500.12123/16588
network_acronym_str INTADig
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network_name_str INTA Digital (INTA)
spelling Bacillus thuringiensis Bt_UNVM-84, a Novel Strain Showing Insecticidal Activity against Anthonomus grandis Boheman (Coleoptera: Curculionidae)Sauka, Diego HernanPeralta, CeciliaPérez, Melisa PaulaMolla, AntonellaFernandez-Göbel, TadeoOcampo, FedericoPalma, LeopoldoBacillus thuringiensisControl BiológicoPlagasBiological ControlPestsInsecticidal ProteinsVpb1/Vpa2 ProteinsCry8 ProteinsBioinsecticidesBacillus thuringiensis is a Gram-positive bacterium known for its insecticidal proteins effective against various insect pests. However, limited strains and proteins target coleopteran pests like Anthonomous grandis Boheman, causing substantial economic losses in the cotton industry. This study focuses on characterizing a Bacillus sp. strain, isolated from Oncativo (Argentina), which exhibits ovoid to amorphous parasporal crystals and was designated Bt_UNVM-84. Its genome encodes genes for the production of two pairs of binary Vpb1/Vpa2 proteins and three Cry-like proteins showing similarity with different Cry8 proteins. Interestingly, this gene content was found to be conserved in a previously characterized Argentine isolate of B. thuringiensis designated INTA Fr7-4. SDS-PAGE analysis revealed a major band of 130 kDa that is proteolytically processed to an approximately 66-kDa protein fragment by trypsin. Bioassays performed with spore-crystal mixtures demonstrated an interesting insecticidal activity against the cotton boll weevil A. grandis neonate larvae, resulting in 91% mortality. Strain Bt_UNVM-84 is, therefore, an interesting candidate for the efficient biological control of this species, causing significant economic losses in the cotton industry in the Americas.Instituto de Microbiología y Zoología Agrícola (IMYZA)Fil: Sauka, Diego Herman. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Microbiología y Zoología Agrícola; ArgentinaFil: Sauka, Diego Herman. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Peralta, Cecilia. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Peralta, Cecilia. Universidad Nacional de Villa María. Instituto Multidisciplinario de Investigación y Transferencia Agroalimentaria y Biotecnológica; ArgentinaFil: Peralta, Cecilia. Universitat de València. Instituto BIOTECMED. Departamento de Genética. Laboratorio de Control Biotecnológico de Plagas; EspañaFil: Pérez, Melisa Paula. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Microbiología y Zoología Agrícola; ArgentinaFil: Molla, Antonella. Universidad Nacional de Villa María. Instituto Multidisciplinario de Investigación y Transferencia Agroalimentaria y Biotecnológica; ArgentinaFil: Fernandez-Göbel, Tadeo. Elytron Biotech S.A.; ArgentinaFil: Ocampo, Federico. Elytron Biotech S.A.; ArgentinaFil: Palma, Leopoldo. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Palma, Leopoldo. Universidad Nacional de Villa María. Instituto Multidisciplinario de Investigación y Transferencia Agroalimentaria y Biotecnológica; ArgentinaFil: Palma, Leopoldo. Universitat de València. Instituto BIOTECMED. Departamento de Genética. Laboratorio de Control Biotecnológico de Plagas; EspañaMDPI2024-02-14T11:26:36Z2024-02-14T11:26:36Z2024-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttp://hdl.handle.net/20.500.12123/16588https://www.mdpi.com/2072-6651/16/1/42072-6651https://doi.org/10.3390/toxins16010004Toxins 16 (1) : 4 (January 2024)reponame:INTA Digital (INTA)instname:Instituto Nacional de Tecnología Agropecuariaenginfo:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by-nc-sa/4.0/Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)2025-09-04T09:50:11Zoai:localhost:20.500.12123/16588instacron:INTAInstitucionalhttp://repositorio.inta.gob.ar/Organismo científico-tecnológicoNo correspondehttp://repositorio.inta.gob.ar/oai/requesttripaldi.nicolas@inta.gob.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:l2025-09-04 09:50:11.904INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuariafalse
dc.title.none.fl_str_mv Bacillus thuringiensis Bt_UNVM-84, a Novel Strain Showing Insecticidal Activity against Anthonomus grandis Boheman (Coleoptera: Curculionidae)
title Bacillus thuringiensis Bt_UNVM-84, a Novel Strain Showing Insecticidal Activity against Anthonomus grandis Boheman (Coleoptera: Curculionidae)
spellingShingle Bacillus thuringiensis Bt_UNVM-84, a Novel Strain Showing Insecticidal Activity against Anthonomus grandis Boheman (Coleoptera: Curculionidae)
Sauka, Diego Hernan
Bacillus thuringiensis
Control Biológico
Plagas
Biological Control
Pests
Insecticidal Proteins
Vpb1/Vpa2 Proteins
Cry8 Proteins
Bioinsecticides
title_short Bacillus thuringiensis Bt_UNVM-84, a Novel Strain Showing Insecticidal Activity against Anthonomus grandis Boheman (Coleoptera: Curculionidae)
title_full Bacillus thuringiensis Bt_UNVM-84, a Novel Strain Showing Insecticidal Activity against Anthonomus grandis Boheman (Coleoptera: Curculionidae)
title_fullStr Bacillus thuringiensis Bt_UNVM-84, a Novel Strain Showing Insecticidal Activity against Anthonomus grandis Boheman (Coleoptera: Curculionidae)
title_full_unstemmed Bacillus thuringiensis Bt_UNVM-84, a Novel Strain Showing Insecticidal Activity against Anthonomus grandis Boheman (Coleoptera: Curculionidae)
title_sort Bacillus thuringiensis Bt_UNVM-84, a Novel Strain Showing Insecticidal Activity against Anthonomus grandis Boheman (Coleoptera: Curculionidae)
dc.creator.none.fl_str_mv Sauka, Diego Hernan
Peralta, Cecilia
Pérez, Melisa Paula
Molla, Antonella
Fernandez-Göbel, Tadeo
Ocampo, Federico
Palma, Leopoldo
author Sauka, Diego Hernan
author_facet Sauka, Diego Hernan
Peralta, Cecilia
Pérez, Melisa Paula
Molla, Antonella
Fernandez-Göbel, Tadeo
Ocampo, Federico
Palma, Leopoldo
author_role author
author2 Peralta, Cecilia
Pérez, Melisa Paula
Molla, Antonella
Fernandez-Göbel, Tadeo
Ocampo, Federico
Palma, Leopoldo
author2_role author
author
author
author
author
author
dc.subject.none.fl_str_mv Bacillus thuringiensis
Control Biológico
Plagas
Biological Control
Pests
Insecticidal Proteins
Vpb1/Vpa2 Proteins
Cry8 Proteins
Bioinsecticides
topic Bacillus thuringiensis
Control Biológico
Plagas
Biological Control
Pests
Insecticidal Proteins
Vpb1/Vpa2 Proteins
Cry8 Proteins
Bioinsecticides
dc.description.none.fl_txt_mv Bacillus thuringiensis is a Gram-positive bacterium known for its insecticidal proteins effective against various insect pests. However, limited strains and proteins target coleopteran pests like Anthonomous grandis Boheman, causing substantial economic losses in the cotton industry. This study focuses on characterizing a Bacillus sp. strain, isolated from Oncativo (Argentina), which exhibits ovoid to amorphous parasporal crystals and was designated Bt_UNVM-84. Its genome encodes genes for the production of two pairs of binary Vpb1/Vpa2 proteins and three Cry-like proteins showing similarity with different Cry8 proteins. Interestingly, this gene content was found to be conserved in a previously characterized Argentine isolate of B. thuringiensis designated INTA Fr7-4. SDS-PAGE analysis revealed a major band of 130 kDa that is proteolytically processed to an approximately 66-kDa protein fragment by trypsin. Bioassays performed with spore-crystal mixtures demonstrated an interesting insecticidal activity against the cotton boll weevil A. grandis neonate larvae, resulting in 91% mortality. Strain Bt_UNVM-84 is, therefore, an interesting candidate for the efficient biological control of this species, causing significant economic losses in the cotton industry in the Americas.
Instituto de Microbiología y Zoología Agrícola (IMYZA)
Fil: Sauka, Diego Herman. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Microbiología y Zoología Agrícola; Argentina
Fil: Sauka, Diego Herman. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Peralta, Cecilia. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Peralta, Cecilia. Universidad Nacional de Villa María. Instituto Multidisciplinario de Investigación y Transferencia Agroalimentaria y Biotecnológica; Argentina
Fil: Peralta, Cecilia. Universitat de València. Instituto BIOTECMED. Departamento de Genética. Laboratorio de Control Biotecnológico de Plagas; España
Fil: Pérez, Melisa Paula. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Microbiología y Zoología Agrícola; Argentina
Fil: Molla, Antonella. Universidad Nacional de Villa María. Instituto Multidisciplinario de Investigación y Transferencia Agroalimentaria y Biotecnológica; Argentina
Fil: Fernandez-Göbel, Tadeo. Elytron Biotech S.A.; Argentina
Fil: Ocampo, Federico. Elytron Biotech S.A.; Argentina
Fil: Palma, Leopoldo. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Palma, Leopoldo. Universidad Nacional de Villa María. Instituto Multidisciplinario de Investigación y Transferencia Agroalimentaria y Biotecnológica; Argentina
Fil: Palma, Leopoldo. Universitat de València. Instituto BIOTECMED. Departamento de Genética. Laboratorio de Control Biotecnológico de Plagas; España
description Bacillus thuringiensis is a Gram-positive bacterium known for its insecticidal proteins effective against various insect pests. However, limited strains and proteins target coleopteran pests like Anthonomous grandis Boheman, causing substantial economic losses in the cotton industry. This study focuses on characterizing a Bacillus sp. strain, isolated from Oncativo (Argentina), which exhibits ovoid to amorphous parasporal crystals and was designated Bt_UNVM-84. Its genome encodes genes for the production of two pairs of binary Vpb1/Vpa2 proteins and three Cry-like proteins showing similarity with different Cry8 proteins. Interestingly, this gene content was found to be conserved in a previously characterized Argentine isolate of B. thuringiensis designated INTA Fr7-4. SDS-PAGE analysis revealed a major band of 130 kDa that is proteolytically processed to an approximately 66-kDa protein fragment by trypsin. Bioassays performed with spore-crystal mixtures demonstrated an interesting insecticidal activity against the cotton boll weevil A. grandis neonate larvae, resulting in 91% mortality. Strain Bt_UNVM-84 is, therefore, an interesting candidate for the efficient biological control of this species, causing significant economic losses in the cotton industry in the Americas.
publishDate 2024
dc.date.none.fl_str_mv 2024-02-14T11:26:36Z
2024-02-14T11:26:36Z
2024-01
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/20.500.12123/16588
https://www.mdpi.com/2072-6651/16/1/4
2072-6651
https://doi.org/10.3390/toxins16010004
url http://hdl.handle.net/20.500.12123/16588
https://www.mdpi.com/2072-6651/16/1/4
https://doi.org/10.3390/toxins16010004
identifier_str_mv 2072-6651
dc.language.none.fl_str_mv eng
language eng
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
eu_rights_str_mv openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv MDPI
publisher.none.fl_str_mv MDPI
dc.source.none.fl_str_mv Toxins 16 (1) : 4 (January 2024)
reponame:INTA Digital (INTA)
instname:Instituto Nacional de Tecnología Agropecuaria
reponame_str INTA Digital (INTA)
collection INTA Digital (INTA)
instname_str Instituto Nacional de Tecnología Agropecuaria
repository.name.fl_str_mv INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuaria
repository.mail.fl_str_mv tripaldi.nicolas@inta.gob.ar
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