Adesmia boronioides and Solidago chilensis, two novels herbal infusions with toxic effects against colon cancer derived cells

Autores
Gastaldi, Bruno; Assef, Yanina Andrea; González, Silvia Beatriz; Marino, Gabriela Inés
Año de publicación
2018
Idioma
inglés
Tipo de recurso
documento de conferencia
Estado
versión publicada
Descripción
The development and progression of colon cancer is strongly influenced by diet substances that enter in the digestive tract. Herbalinfusions from medicinal plants usually contains phytochemicalsthat can restrain the development and progression of colon cancerin various ways. Flavonoids, an important group of these phytochemicals, report a recognized anti-inflammatory, antioxidant andsignal-regulating properties. Adesmia boronioides and Solidagochilensis are two native medicinal plants that contain flavonoids andhave reported promising antiproliferative activity against T-84 cells.Our aim was to study the toxic effects of herbal infusions obtainedfrom A. boronioides and S. chilensis on Caco-2 and HT-29 cells asmodels of colon cancer.We observed by MTT assay (after 72 h) that the percentage ofviable cells decreased with the increase in the concentration offreeze-dried infusions of both plant species (0 to 50 mg/ml) (p<0.05,n= 3). S. chilensis had a higher antiproliferative effect (EC50 (mg/ml): 0.57±0.06 and 0.18±0.02) in comparison with A. boronioides(EC50 (mg/ml): 1.27±0.08 and 2.87±0.21), for Caco-2 and HT-29cells, respectively. Colchicine was used as positive control. Similarresults were obtained by Trypan blue exclusion technique (p<0.05,n= 3). After staining the cells with AO and Et/Br, apoptosis cells(orange cells) were observed under the fluorescence microscope.The basal apoptotic percentage (24h) was increased in A. boronioides (35.0±4.1 and 46.2±8.2%) and in S. chilensis (47.2±6.7 and35.8±4.0%) with respect to control (3.8±4.9 and 2.7±3.1%) in Caco2 and HT-29, respectively (p<0.05, n=3). The Procaspase-3 expression was also checked.We conclude that the infusion of the both species exert strong antiproliferative activity on cells derived from colon cancer, partly dueto the modulation of basal apoptosis. Its effects show to be muchgreater in comparison with other species studied. These results provide a direction for further researches about the antitumoral potentialof these native plants.
Fil: Gastaldi, Bruno. Universidad Nacional de la Patagonia "San Juan Bosco". Facultad de Ciencias Naturales - Sede Esquel; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Assef, Yanina Andrea. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Patagonia Norte. Centro de Investigación Esquel de Montaña y Estepa Patagónica. Universidad Nacional de la Patagonia "San Juan Bosco". Centro de Investigación Esquel de Montaña y Estepa Patagónica; Argentina
Fil: González, Silvia Beatriz. Universidad Nacional de la Patagonia "San Juan Bosco". Facultad de Ciencias Naturales - Sede Esquel; Argentina
Fil: Marino, Gabriela Inés. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones Médicas. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Investigaciones Médicas; Argentina
LXII Reunión Anual de la Sociedad Argentina de Investigación Clínica; LXVI Reunión Anual de la Sociedad Argentina de Inmunología; Reunión Anual de la Sociedad Argentina de Fisiología
Mar del Plata
Argentina
Sociedad Argentina de Inmunología
Sociedad Argentina de Fisiología
Sociedad Argentina de Investigación Clínica
Materia
ADESMIA BORONIOIDES
SOLIDAGO CHILENSIS
HERBAL INFUSIONS
TOXIC EFFECTS
COLON CANCER DERIVED CELLS
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/154719
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/154719
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Adesmia boronioides and Solidago chilensis, two novels herbal infusions with toxic effects against colon cancer derived cellsGastaldi, BrunoAssef, Yanina AndreaGonzález, Silvia BeatrizMarino, Gabriela InésADESMIA BORONIOIDESSOLIDAGO CHILENSISHERBAL INFUSIONSTOXIC EFFECTSCOLON CANCER DERIVED CELLShttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1https://purl.org/becyt/ford/3.1https://purl.org/becyt/ford/3The development and progression of colon cancer is strongly influenced by diet substances that enter in the digestive tract. Herbalinfusions from medicinal plants usually contains phytochemicalsthat can restrain the development and progression of colon cancerin various ways. Flavonoids, an important group of these phytochemicals, report a recognized anti-inflammatory, antioxidant andsignal-regulating properties. Adesmia boronioides and Solidagochilensis are two native medicinal plants that contain flavonoids andhave reported promising antiproliferative activity against T-84 cells.Our aim was to study the toxic effects of herbal infusions obtainedfrom A. boronioides and S. chilensis on Caco-2 and HT-29 cells asmodels of colon cancer.We observed by MTT assay (after 72 h) that the percentage ofviable cells decreased with the increase in the concentration offreeze-dried infusions of both plant species (0 to 50 mg/ml) (p<0.05,n= 3). S. chilensis had a higher antiproliferative effect (EC50 (mg/ml): 0.57±0.06 and 0.18±0.02) in comparison with A. boronioides(EC50 (mg/ml): 1.27±0.08 and 2.87±0.21), for Caco-2 and HT-29cells, respectively. Colchicine was used as positive control. Similarresults were obtained by Trypan blue exclusion technique (p<0.05,n= 3). After staining the cells with AO and Et/Br, apoptosis cells(orange cells) were observed under the fluorescence microscope.The basal apoptotic percentage (24h) was increased in A. boronioides (35.0±4.1 and 46.2±8.2%) and in S. chilensis (47.2±6.7 and35.8±4.0%) with respect to control (3.8±4.9 and 2.7±3.1%) in Caco2 and HT-29, respectively (p<0.05, n=3). The Procaspase-3 expression was also checked.We conclude that the infusion of the both species exert strong antiproliferative activity on cells derived from colon cancer, partly dueto the modulation of basal apoptosis. Its effects show to be muchgreater in comparison with other species studied. These results provide a direction for further researches about the antitumoral potentialof these native plants.Fil: Gastaldi, Bruno. Universidad Nacional de la Patagonia "San Juan Bosco". Facultad de Ciencias Naturales - Sede Esquel; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Assef, Yanina Andrea. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Patagonia Norte. Centro de Investigación Esquel de Montaña y Estepa Patagónica. Universidad Nacional de la Patagonia "San Juan Bosco". Centro de Investigación Esquel de Montaña y Estepa Patagónica; ArgentinaFil: González, Silvia Beatriz. Universidad Nacional de la Patagonia "San Juan Bosco". Facultad de Ciencias Naturales - Sede Esquel; ArgentinaFil: Marino, Gabriela Inés. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones Médicas. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Investigaciones Médicas; ArgentinaLXII Reunión Anual de la Sociedad Argentina de Investigación Clínica; LXVI Reunión Anual de la Sociedad Argentina de Inmunología; Reunión Anual de la Sociedad Argentina de FisiologíaMar del PlataArgentinaSociedad Argentina de InmunologíaSociedad Argentina de FisiologíaSociedad Argentina de Investigación ClínicaFundación Revista MedicinaPerez Leiros, ClaudiaBaldi, Pablo CesarCrottogini, Alberto Jose2018info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/conferenceObjectReuniónJournalhttp://purl.org/coar/resource_type/c_5794info:ar-repo/semantics/documentoDeConferenciaapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/154719Adesmia boronioides and Solidago chilensis, two novels herbal infusions with toxic effects against colon cancer derived cells; LXII Reunión Anual de la Sociedad Argentina de Investigación Clínica; LXVI Reunión Anual de la Sociedad Argentina de Inmunología; Reunión Anual de la Sociedad Argentina de Fisiología; Mar del Plata; Argentina; 2018; 1-40025-76801669-9106CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.saic.org.ar/reunion-anualNacionalinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-11-05T10:46:31Zoai:ri.conicet.gov.ar:11336/154719instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-11-05 10:46:31.953CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Adesmia boronioides and Solidago chilensis, two novels herbal infusions with toxic effects against colon cancer derived cells
title Adesmia boronioides and Solidago chilensis, two novels herbal infusions with toxic effects against colon cancer derived cells
spellingShingle Adesmia boronioides and Solidago chilensis, two novels herbal infusions with toxic effects against colon cancer derived cells
Gastaldi, Bruno
ADESMIA BORONIOIDES
SOLIDAGO CHILENSIS
HERBAL INFUSIONS
TOXIC EFFECTS
COLON CANCER DERIVED CELLS
title_short Adesmia boronioides and Solidago chilensis, two novels herbal infusions with toxic effects against colon cancer derived cells
title_full Adesmia boronioides and Solidago chilensis, two novels herbal infusions with toxic effects against colon cancer derived cells
title_fullStr Adesmia boronioides and Solidago chilensis, two novels herbal infusions with toxic effects against colon cancer derived cells
title_full_unstemmed Adesmia boronioides and Solidago chilensis, two novels herbal infusions with toxic effects against colon cancer derived cells
title_sort Adesmia boronioides and Solidago chilensis, two novels herbal infusions with toxic effects against colon cancer derived cells
dc.creator.none.fl_str_mv Gastaldi, Bruno
Assef, Yanina Andrea
González, Silvia Beatriz
Marino, Gabriela Inés
author Gastaldi, Bruno
author_facet Gastaldi, Bruno
Assef, Yanina Andrea
González, Silvia Beatriz
Marino, Gabriela Inés
author_role author
author2 Assef, Yanina Andrea
González, Silvia Beatriz
Marino, Gabriela Inés
author2_role author
author
author
dc.contributor.none.fl_str_mv Perez Leiros, Claudia
Baldi, Pablo Cesar
Crottogini, Alberto Jose
dc.subject.none.fl_str_mv ADESMIA BORONIOIDES
SOLIDAGO CHILENSIS
HERBAL INFUSIONS
TOXIC EFFECTS
COLON CANCER DERIVED CELLS
topic ADESMIA BORONIOIDES
SOLIDAGO CHILENSIS
HERBAL INFUSIONS
TOXIC EFFECTS
COLON CANCER DERIVED CELLS
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
https://purl.org/becyt/ford/3.1
https://purl.org/becyt/ford/3
dc.description.none.fl_txt_mv The development and progression of colon cancer is strongly influenced by diet substances that enter in the digestive tract. Herbalinfusions from medicinal plants usually contains phytochemicalsthat can restrain the development and progression of colon cancerin various ways. Flavonoids, an important group of these phytochemicals, report a recognized anti-inflammatory, antioxidant andsignal-regulating properties. Adesmia boronioides and Solidagochilensis are two native medicinal plants that contain flavonoids andhave reported promising antiproliferative activity against T-84 cells.Our aim was to study the toxic effects of herbal infusions obtainedfrom A. boronioides and S. chilensis on Caco-2 and HT-29 cells asmodels of colon cancer.We observed by MTT assay (after 72 h) that the percentage ofviable cells decreased with the increase in the concentration offreeze-dried infusions of both plant species (0 to 50 mg/ml) (p<0.05,n= 3). S. chilensis had a higher antiproliferative effect (EC50 (mg/ml): 0.57±0.06 and 0.18±0.02) in comparison with A. boronioides(EC50 (mg/ml): 1.27±0.08 and 2.87±0.21), for Caco-2 and HT-29cells, respectively. Colchicine was used as positive control. Similarresults were obtained by Trypan blue exclusion technique (p<0.05,n= 3). After staining the cells with AO and Et/Br, apoptosis cells(orange cells) were observed under the fluorescence microscope.The basal apoptotic percentage (24h) was increased in A. boronioides (35.0±4.1 and 46.2±8.2%) and in S. chilensis (47.2±6.7 and35.8±4.0%) with respect to control (3.8±4.9 and 2.7±3.1%) in Caco2 and HT-29, respectively (p<0.05, n=3). The Procaspase-3 expression was also checked.We conclude that the infusion of the both species exert strong antiproliferative activity on cells derived from colon cancer, partly dueto the modulation of basal apoptosis. Its effects show to be muchgreater in comparison with other species studied. These results provide a direction for further researches about the antitumoral potentialof these native plants.
Fil: Gastaldi, Bruno. Universidad Nacional de la Patagonia "San Juan Bosco". Facultad de Ciencias Naturales - Sede Esquel; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Assef, Yanina Andrea. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Patagonia Norte. Centro de Investigación Esquel de Montaña y Estepa Patagónica. Universidad Nacional de la Patagonia "San Juan Bosco". Centro de Investigación Esquel de Montaña y Estepa Patagónica; Argentina
Fil: González, Silvia Beatriz. Universidad Nacional de la Patagonia "San Juan Bosco". Facultad de Ciencias Naturales - Sede Esquel; Argentina
Fil: Marino, Gabriela Inés. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones Médicas. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Investigaciones Médicas; Argentina
LXII Reunión Anual de la Sociedad Argentina de Investigación Clínica; LXVI Reunión Anual de la Sociedad Argentina de Inmunología; Reunión Anual de la Sociedad Argentina de Fisiología
Mar del Plata
Argentina
Sociedad Argentina de Inmunología
Sociedad Argentina de Fisiología
Sociedad Argentina de Investigación Clínica
description The development and progression of colon cancer is strongly influenced by diet substances that enter in the digestive tract. Herbalinfusions from medicinal plants usually contains phytochemicalsthat can restrain the development and progression of colon cancerin various ways. Flavonoids, an important group of these phytochemicals, report a recognized anti-inflammatory, antioxidant andsignal-regulating properties. Adesmia boronioides and Solidagochilensis are two native medicinal plants that contain flavonoids andhave reported promising antiproliferative activity against T-84 cells.Our aim was to study the toxic effects of herbal infusions obtainedfrom A. boronioides and S. chilensis on Caco-2 and HT-29 cells asmodels of colon cancer.We observed by MTT assay (after 72 h) that the percentage ofviable cells decreased with the increase in the concentration offreeze-dried infusions of both plant species (0 to 50 mg/ml) (p<0.05,n= 3). S. chilensis had a higher antiproliferative effect (EC50 (mg/ml): 0.57±0.06 and 0.18±0.02) in comparison with A. boronioides(EC50 (mg/ml): 1.27±0.08 and 2.87±0.21), for Caco-2 and HT-29cells, respectively. Colchicine was used as positive control. Similarresults were obtained by Trypan blue exclusion technique (p<0.05,n= 3). After staining the cells with AO and Et/Br, apoptosis cells(orange cells) were observed under the fluorescence microscope.The basal apoptotic percentage (24h) was increased in A. boronioides (35.0±4.1 and 46.2±8.2%) and in S. chilensis (47.2±6.7 and35.8±4.0%) with respect to control (3.8±4.9 and 2.7±3.1%) in Caco2 and HT-29, respectively (p<0.05, n=3). The Procaspase-3 expression was also checked.We conclude that the infusion of the both species exert strong antiproliferative activity on cells derived from colon cancer, partly dueto the modulation of basal apoptosis. Its effects show to be muchgreater in comparison with other species studied. These results provide a direction for further researches about the antitumoral potentialof these native plants.
publishDate 2018
dc.date.none.fl_str_mv 2018
dc.type.none.fl_str_mv info:eu-repo/semantics/publishedVersion
info:eu-repo/semantics/conferenceObject
Reunión
Journal
http://purl.org/coar/resource_type/c_5794
info:ar-repo/semantics/documentoDeConferencia
status_str publishedVersion
format conferenceObject
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/154719
Adesmia boronioides and Solidago chilensis, two novels herbal infusions with toxic effects against colon cancer derived cells; LXII Reunión Anual de la Sociedad Argentina de Investigación Clínica; LXVI Reunión Anual de la Sociedad Argentina de Inmunología; Reunión Anual de la Sociedad Argentina de Fisiología; Mar del Plata; Argentina; 2018; 1-4
0025-7680
1669-9106
CONICET Digital
CONICET
url http://hdl.handle.net/11336/154719
identifier_str_mv Adesmia boronioides and Solidago chilensis, two novels herbal infusions with toxic effects against colon cancer derived cells; LXII Reunión Anual de la Sociedad Argentina de Investigación Clínica; LXVI Reunión Anual de la Sociedad Argentina de Inmunología; Reunión Anual de la Sociedad Argentina de Fisiología; Mar del Plata; Argentina; 2018; 1-4
0025-7680
1669-9106
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
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dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
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dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
dc.coverage.none.fl_str_mv Nacional
dc.publisher.none.fl_str_mv Fundación Revista Medicina
publisher.none.fl_str_mv Fundación Revista Medicina
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
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reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.121305

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