Protein purification by affinity chromatography with peptide ligands selected from the screening of combinatorial libraries
- Autores
- Camperi, Silvia Andrea; Marani, Mariela Mirta; Martínez Ceron, María Camila; Albericio, Fernando; Cascone, Osvaldo
- Año de publicación
- 2010
- Idioma
- inglés
- Tipo de recurso
- artículo
- Estado
- versión publicada
- Descripción
- Affinity chromatography is likely to play an everincreasing important role in protein purification as it is the most effective method for the direct isolation and purification of biomolecules from complex mixtures. Successful separation by affinity chromatography requires the availability of a selective ligand. Short peptides are excellent ligands for affinity separations as they have higher selectivity than dyes and metals, they are more stable than antibodies to elution and cleaning conditions, and they are not likely to cause an immune response in case of leakage into the product. Furthermore, the combinatorial synthesis of peptide libraries allows obtaining millions of peptides, thus greatly facilitating the discovery of suitable affinity ligands for any given protein of interest. After screening of the library the peptides with affinity for the target protein can be identified, typically by Edman microsequencing or mass spectrometry in the case of synthetic libraries, or by DNA sequencing in the case of biological libraries. Numerous proteins have been purified in only one step with chromatographic matrices made of peptide-ligands selected from the screening of combinatorial libraries, attached to different supports.
Fil: Camperi, Silvia Andrea. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Microbiología, Inmunología y Biotecnología. Cátedra de Microbiología Industrial y Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Marani, Mariela Mirta. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Microbiología, Inmunología y Biotecnología. Cátedra de Microbiología Industrial y Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Martínez Ceron, María Camila. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Microbiología, Inmunología y Biotecnología. Cátedra de Microbiología Industrial y Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Albericio, Fernando. Universidad de Barcelona; España
Fil: Cascone, Osvaldo. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Microbiología, Inmunología y Biotecnología. Cátedra de Microbiología Industrial y Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina - Materia
-
Phage Display
One Bead One Compound
Split Mix Split
Divide Couple Recombine
Purification
Combinatorial Library
Peptide
Chromatography - Nivel de accesibilidad
- acceso abierto
- Condiciones de uso
- https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
- Repositorio
- Institución
- Consejo Nacional de Investigaciones Científicas y Técnicas
- OAI Identificador
- oai:ri.conicet.gov.ar:11336/14495
Ver los metadatos del registro completo
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Protein purification by affinity chromatography with peptide ligands selected from the screening of combinatorial librariesCamperi, Silvia AndreaMarani, Mariela MirtaMartínez Ceron, María CamilaAlbericio, FernandoCascone, OsvaldoPhage DisplayOne Bead One CompoundSplit Mix SplitDivide Couple RecombinePurificationCombinatorial LibraryPeptideChromatographyhttps://purl.org/becyt/ford/2.11https://purl.org/becyt/ford/2Affinity chromatography is likely to play an everincreasing important role in protein purification as it is the most effective method for the direct isolation and purification of biomolecules from complex mixtures. Successful separation by affinity chromatography requires the availability of a selective ligand. Short peptides are excellent ligands for affinity separations as they have higher selectivity than dyes and metals, they are more stable than antibodies to elution and cleaning conditions, and they are not likely to cause an immune response in case of leakage into the product. Furthermore, the combinatorial synthesis of peptide libraries allows obtaining millions of peptides, thus greatly facilitating the discovery of suitable affinity ligands for any given protein of interest. After screening of the library the peptides with affinity for the target protein can be identified, typically by Edman microsequencing or mass spectrometry in the case of synthetic libraries, or by DNA sequencing in the case of biological libraries. Numerous proteins have been purified in only one step with chromatographic matrices made of peptide-ligands selected from the screening of combinatorial libraries, attached to different supports.Fil: Camperi, Silvia Andrea. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Microbiología, Inmunología y Biotecnología. Cátedra de Microbiología Industrial y Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Marani, Mariela Mirta. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Microbiología, Inmunología y Biotecnología. Cátedra de Microbiología Industrial y Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Martínez Ceron, María Camila. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Microbiología, Inmunología y Biotecnología. Cátedra de Microbiología Industrial y Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Albericio, Fernando. Universidad de Barcelona; EspañaFil: Cascone, Osvaldo. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Microbiología, Inmunología y Biotecnología. Cátedra de Microbiología Industrial y Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaResearch Trends2010-12info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/14495Camperi, Silvia Andrea; Marani, Mariela Mirta; Martínez Ceron, María Camila; Albericio, Fernando; Cascone, Osvaldo; Protein purification by affinity chromatography with peptide ligands selected from the screening of combinatorial libraries; Research Trends; Trends in Chromatography; 6; 12-2010; 11-220972-8635enginfo:eu-repo/semantics/altIdentifier/url/http://www.researchtrends.net/tia/abstract.asp?in=0&vn=6&tid=60&aid=3090&pub=2010&type=3info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T09:35:46Zoai:ri.conicet.gov.ar:11336/14495instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 09:35:47.165CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse |
dc.title.none.fl_str_mv |
Protein purification by affinity chromatography with peptide ligands selected from the screening of combinatorial libraries |
title |
Protein purification by affinity chromatography with peptide ligands selected from the screening of combinatorial libraries |
spellingShingle |
Protein purification by affinity chromatography with peptide ligands selected from the screening of combinatorial libraries Camperi, Silvia Andrea Phage Display One Bead One Compound Split Mix Split Divide Couple Recombine Purification Combinatorial Library Peptide Chromatography |
title_short |
Protein purification by affinity chromatography with peptide ligands selected from the screening of combinatorial libraries |
title_full |
Protein purification by affinity chromatography with peptide ligands selected from the screening of combinatorial libraries |
title_fullStr |
Protein purification by affinity chromatography with peptide ligands selected from the screening of combinatorial libraries |
title_full_unstemmed |
Protein purification by affinity chromatography with peptide ligands selected from the screening of combinatorial libraries |
title_sort |
Protein purification by affinity chromatography with peptide ligands selected from the screening of combinatorial libraries |
dc.creator.none.fl_str_mv |
Camperi, Silvia Andrea Marani, Mariela Mirta Martínez Ceron, María Camila Albericio, Fernando Cascone, Osvaldo |
author |
Camperi, Silvia Andrea |
author_facet |
Camperi, Silvia Andrea Marani, Mariela Mirta Martínez Ceron, María Camila Albericio, Fernando Cascone, Osvaldo |
author_role |
author |
author2 |
Marani, Mariela Mirta Martínez Ceron, María Camila Albericio, Fernando Cascone, Osvaldo |
author2_role |
author author author author |
dc.subject.none.fl_str_mv |
Phage Display One Bead One Compound Split Mix Split Divide Couple Recombine Purification Combinatorial Library Peptide Chromatography |
topic |
Phage Display One Bead One Compound Split Mix Split Divide Couple Recombine Purification Combinatorial Library Peptide Chromatography |
purl_subject.fl_str_mv |
https://purl.org/becyt/ford/2.11 https://purl.org/becyt/ford/2 |
dc.description.none.fl_txt_mv |
Affinity chromatography is likely to play an everincreasing important role in protein purification as it is the most effective method for the direct isolation and purification of biomolecules from complex mixtures. Successful separation by affinity chromatography requires the availability of a selective ligand. Short peptides are excellent ligands for affinity separations as they have higher selectivity than dyes and metals, they are more stable than antibodies to elution and cleaning conditions, and they are not likely to cause an immune response in case of leakage into the product. Furthermore, the combinatorial synthesis of peptide libraries allows obtaining millions of peptides, thus greatly facilitating the discovery of suitable affinity ligands for any given protein of interest. After screening of the library the peptides with affinity for the target protein can be identified, typically by Edman microsequencing or mass spectrometry in the case of synthetic libraries, or by DNA sequencing in the case of biological libraries. Numerous proteins have been purified in only one step with chromatographic matrices made of peptide-ligands selected from the screening of combinatorial libraries, attached to different supports. Fil: Camperi, Silvia Andrea. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Microbiología, Inmunología y Biotecnología. Cátedra de Microbiología Industrial y Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Marani, Mariela Mirta. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Microbiología, Inmunología y Biotecnología. Cátedra de Microbiología Industrial y Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Martínez Ceron, María Camila. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Microbiología, Inmunología y Biotecnología. Cátedra de Microbiología Industrial y Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Albericio, Fernando. Universidad de Barcelona; España Fil: Cascone, Osvaldo. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Microbiología, Inmunología y Biotecnología. Cátedra de Microbiología Industrial y Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina |
description |
Affinity chromatography is likely to play an everincreasing important role in protein purification as it is the most effective method for the direct isolation and purification of biomolecules from complex mixtures. Successful separation by affinity chromatography requires the availability of a selective ligand. Short peptides are excellent ligands for affinity separations as they have higher selectivity than dyes and metals, they are more stable than antibodies to elution and cleaning conditions, and they are not likely to cause an immune response in case of leakage into the product. Furthermore, the combinatorial synthesis of peptide libraries allows obtaining millions of peptides, thus greatly facilitating the discovery of suitable affinity ligands for any given protein of interest. After screening of the library the peptides with affinity for the target protein can be identified, typically by Edman microsequencing or mass spectrometry in the case of synthetic libraries, or by DNA sequencing in the case of biological libraries. Numerous proteins have been purified in only one step with chromatographic matrices made of peptide-ligands selected from the screening of combinatorial libraries, attached to different supports. |
publishDate |
2010 |
dc.date.none.fl_str_mv |
2010-12 |
dc.type.none.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://purl.org/coar/resource_type/c_6501 info:ar-repo/semantics/articulo |
format |
article |
status_str |
publishedVersion |
dc.identifier.none.fl_str_mv |
http://hdl.handle.net/11336/14495 Camperi, Silvia Andrea; Marani, Mariela Mirta; Martínez Ceron, María Camila; Albericio, Fernando; Cascone, Osvaldo; Protein purification by affinity chromatography with peptide ligands selected from the screening of combinatorial libraries; Research Trends; Trends in Chromatography; 6; 12-2010; 11-22 0972-8635 |
url |
http://hdl.handle.net/11336/14495 |
identifier_str_mv |
Camperi, Silvia Andrea; Marani, Mariela Mirta; Martínez Ceron, María Camila; Albericio, Fernando; Cascone, Osvaldo; Protein purification by affinity chromatography with peptide ligands selected from the screening of combinatorial libraries; Research Trends; Trends in Chromatography; 6; 12-2010; 11-22 0972-8635 |
dc.language.none.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
info:eu-repo/semantics/altIdentifier/url/http://www.researchtrends.net/tia/abstract.asp?in=0&vn=6&tid=60&aid=3090&pub=2010&type=3 |
dc.rights.none.fl_str_mv |
info:eu-repo/semantics/openAccess https://creativecommons.org/licenses/by-nc-sa/2.5/ar/ |
eu_rights_str_mv |
openAccess |
rights_invalid_str_mv |
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/ |
dc.format.none.fl_str_mv |
application/pdf application/pdf |
dc.publisher.none.fl_str_mv |
Research Trends |
publisher.none.fl_str_mv |
Research Trends |
dc.source.none.fl_str_mv |
reponame:CONICET Digital (CONICET) instname:Consejo Nacional de Investigaciones Científicas y Técnicas |
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CONICET Digital (CONICET) |
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CONICET Digital (CONICET) |
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Consejo Nacional de Investigaciones Científicas y Técnicas |
repository.name.fl_str_mv |
CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas |
repository.mail.fl_str_mv |
dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar |
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13.070432 |