Protein purification by affinity chromatography with peptide ligands selected from the screening of combinatorial libraries

Autores
Camperi, Silvia Andrea; Marani, Mariela Mirta; Martínez Ceron, María Camila; Albericio, Fernando; Cascone, Osvaldo
Año de publicación
2010
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Affinity chromatography is likely to play an everincreasing important role in protein purification as it is the most effective method for the direct isolation and purification of biomolecules from complex mixtures. Successful separation by affinity chromatography requires the availability of a selective ligand. Short peptides are excellent ligands for affinity separations as they have higher selectivity than dyes and metals, they are more stable than antibodies to elution and cleaning conditions, and they are not likely to cause an immune response in case of leakage into the product. Furthermore, the combinatorial synthesis of peptide libraries allows obtaining millions of peptides, thus greatly facilitating the discovery of suitable affinity ligands for any given protein of interest. After screening of the library the peptides with affinity for the target protein can be identified, typically by Edman microsequencing or mass spectrometry in the case of synthetic libraries, or by DNA sequencing in the case of biological libraries. Numerous proteins have been purified in only one step with chromatographic matrices made of peptide-ligands selected from the screening of combinatorial libraries, attached to different supports.
Fil: Camperi, Silvia Andrea. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Microbiología, Inmunología y Biotecnología. Cátedra de Microbiología Industrial y Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Marani, Mariela Mirta. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Microbiología, Inmunología y Biotecnología. Cátedra de Microbiología Industrial y Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Martínez Ceron, María Camila. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Microbiología, Inmunología y Biotecnología. Cátedra de Microbiología Industrial y Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Albericio, Fernando. Universidad de Barcelona; España
Fil: Cascone, Osvaldo. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Microbiología, Inmunología y Biotecnología. Cátedra de Microbiología Industrial y Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Materia
Phage Display
One Bead One Compound
Split Mix Split
Divide Couple Recombine
Purification
Combinatorial Library
Peptide
Chromatography
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/14495
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/14495
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Protein purification by affinity chromatography with peptide ligands selected from the screening of combinatorial librariesCamperi, Silvia AndreaMarani, Mariela MirtaMartínez Ceron, María CamilaAlbericio, FernandoCascone, OsvaldoPhage DisplayOne Bead One CompoundSplit Mix SplitDivide Couple RecombinePurificationCombinatorial LibraryPeptideChromatographyhttps://purl.org/becyt/ford/2.11https://purl.org/becyt/ford/2Affinity chromatography is likely to play an everincreasing important role in protein purification as it is the most effective method for the direct isolation and purification of biomolecules from complex mixtures. Successful separation by affinity chromatography requires the availability of a selective ligand. Short peptides are excellent ligands for affinity separations as they have higher selectivity than dyes and metals, they are more stable than antibodies to elution and cleaning conditions, and they are not likely to cause an immune response in case of leakage into the product. Furthermore, the combinatorial synthesis of peptide libraries allows obtaining millions of peptides, thus greatly facilitating the discovery of suitable affinity ligands for any given protein of interest. After screening of the library the peptides with affinity for the target protein can be identified, typically by Edman microsequencing or mass spectrometry in the case of synthetic libraries, or by DNA sequencing in the case of biological libraries. Numerous proteins have been purified in only one step with chromatographic matrices made of peptide-ligands selected from the screening of combinatorial libraries, attached to different supports.Fil: Camperi, Silvia Andrea. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Microbiología, Inmunología y Biotecnología. Cátedra de Microbiología Industrial y Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Marani, Mariela Mirta. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Microbiología, Inmunología y Biotecnología. Cátedra de Microbiología Industrial y Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Martínez Ceron, María Camila. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Microbiología, Inmunología y Biotecnología. Cátedra de Microbiología Industrial y Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Albericio, Fernando. Universidad de Barcelona; EspañaFil: Cascone, Osvaldo. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Microbiología, Inmunología y Biotecnología. Cátedra de Microbiología Industrial y Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaResearch Trends2010-12info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/14495Camperi, Silvia Andrea; Marani, Mariela Mirta; Martínez Ceron, María Camila; Albericio, Fernando; Cascone, Osvaldo; Protein purification by affinity chromatography with peptide ligands selected from the screening of combinatorial libraries; Research Trends; Trends in Chromatography; 6; 12-2010; 11-220972-8635enginfo:eu-repo/semantics/altIdentifier/url/http://www.researchtrends.net/tia/abstract.asp?in=0&vn=6&tid=60&aid=3090&pub=2010&type=3info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-22T11:03:51Zoai:ri.conicet.gov.ar:11336/14495instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-22 11:03:51.732CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Protein purification by affinity chromatography with peptide ligands selected from the screening of combinatorial libraries
title Protein purification by affinity chromatography with peptide ligands selected from the screening of combinatorial libraries
spellingShingle Protein purification by affinity chromatography with peptide ligands selected from the screening of combinatorial libraries
Camperi, Silvia Andrea
Phage Display
One Bead One Compound
Split Mix Split
Divide Couple Recombine
Purification
Combinatorial Library
Peptide
Chromatography
title_short Protein purification by affinity chromatography with peptide ligands selected from the screening of combinatorial libraries
title_full Protein purification by affinity chromatography with peptide ligands selected from the screening of combinatorial libraries
title_fullStr Protein purification by affinity chromatography with peptide ligands selected from the screening of combinatorial libraries
title_full_unstemmed Protein purification by affinity chromatography with peptide ligands selected from the screening of combinatorial libraries
title_sort Protein purification by affinity chromatography with peptide ligands selected from the screening of combinatorial libraries
dc.creator.none.fl_str_mv Camperi, Silvia Andrea
Marani, Mariela Mirta
Martínez Ceron, María Camila
Albericio, Fernando
Cascone, Osvaldo
author Camperi, Silvia Andrea
author_facet Camperi, Silvia Andrea
Marani, Mariela Mirta
Martínez Ceron, María Camila
Albericio, Fernando
Cascone, Osvaldo
author_role author
author2 Marani, Mariela Mirta
Martínez Ceron, María Camila
Albericio, Fernando
Cascone, Osvaldo
author2_role author
author
author
author
dc.subject.none.fl_str_mv Phage Display
One Bead One Compound
Split Mix Split
Divide Couple Recombine
Purification
Combinatorial Library
Peptide
Chromatography
topic Phage Display
One Bead One Compound
Split Mix Split
Divide Couple Recombine
Purification
Combinatorial Library
Peptide
Chromatography
purl_subject.fl_str_mv https://purl.org/becyt/ford/2.11
https://purl.org/becyt/ford/2
dc.description.none.fl_txt_mv Affinity chromatography is likely to play an everincreasing important role in protein purification as it is the most effective method for the direct isolation and purification of biomolecules from complex mixtures. Successful separation by affinity chromatography requires the availability of a selective ligand. Short peptides are excellent ligands for affinity separations as they have higher selectivity than dyes and metals, they are more stable than antibodies to elution and cleaning conditions, and they are not likely to cause an immune response in case of leakage into the product. Furthermore, the combinatorial synthesis of peptide libraries allows obtaining millions of peptides, thus greatly facilitating the discovery of suitable affinity ligands for any given protein of interest. After screening of the library the peptides with affinity for the target protein can be identified, typically by Edman microsequencing or mass spectrometry in the case of synthetic libraries, or by DNA sequencing in the case of biological libraries. Numerous proteins have been purified in only one step with chromatographic matrices made of peptide-ligands selected from the screening of combinatorial libraries, attached to different supports.
Fil: Camperi, Silvia Andrea. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Microbiología, Inmunología y Biotecnología. Cátedra de Microbiología Industrial y Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Marani, Mariela Mirta. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Microbiología, Inmunología y Biotecnología. Cátedra de Microbiología Industrial y Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Martínez Ceron, María Camila. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Microbiología, Inmunología y Biotecnología. Cátedra de Microbiología Industrial y Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Albericio, Fernando. Universidad de Barcelona; España
Fil: Cascone, Osvaldo. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Microbiología, Inmunología y Biotecnología. Cátedra de Microbiología Industrial y Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
description Affinity chromatography is likely to play an everincreasing important role in protein purification as it is the most effective method for the direct isolation and purification of biomolecules from complex mixtures. Successful separation by affinity chromatography requires the availability of a selective ligand. Short peptides are excellent ligands for affinity separations as they have higher selectivity than dyes and metals, they are more stable than antibodies to elution and cleaning conditions, and they are not likely to cause an immune response in case of leakage into the product. Furthermore, the combinatorial synthesis of peptide libraries allows obtaining millions of peptides, thus greatly facilitating the discovery of suitable affinity ligands for any given protein of interest. After screening of the library the peptides with affinity for the target protein can be identified, typically by Edman microsequencing or mass spectrometry in the case of synthetic libraries, or by DNA sequencing in the case of biological libraries. Numerous proteins have been purified in only one step with chromatographic matrices made of peptide-ligands selected from the screening of combinatorial libraries, attached to different supports.
publishDate 2010
dc.date.none.fl_str_mv 2010-12
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/14495
Camperi, Silvia Andrea; Marani, Mariela Mirta; Martínez Ceron, María Camila; Albericio, Fernando; Cascone, Osvaldo; Protein purification by affinity chromatography with peptide ligands selected from the screening of combinatorial libraries; Research Trends; Trends in Chromatography; 6; 12-2010; 11-22
0972-8635
url http://hdl.handle.net/11336/14495
identifier_str_mv Camperi, Silvia Andrea; Marani, Mariela Mirta; Martínez Ceron, María Camila; Albericio, Fernando; Cascone, Osvaldo; Protein purification by affinity chromatography with peptide ligands selected from the screening of combinatorial libraries; Research Trends; Trends in Chromatography; 6; 12-2010; 11-22
0972-8635
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/http://www.researchtrends.net/tia/abstract.asp?in=0&vn=6&tid=60&aid=3090&pub=2010&type=3
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Research Trends
publisher.none.fl_str_mv Research Trends
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1846781281210204160
score 12.982451

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