RIMA-Dependent Nuclear Accumulation of IYO Triggers Auxin-Irreversible Cell Differentiation in Arabidopsis

Autores
Muñoz, Alfonso; Mangano, Silvina; González-García, Mary Paz; Contreras, Ramón; Sauer, Michael; De Rybel, Bert; Weijers, Dolf; Sánchez Serrano, José Juan; Sanmartín, Maite; Rojo, Enrique
Año de publicación
2017
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The transcriptional regulator MINIYO (IYO) is essential and rate-limiting for initiating cell differentiation in Arabidopsis thaliana Moreover, IYO moves from the cytosol into the nucleus in cells at the meristem periphery, possibly triggering their differentiation. However, the genetic mechanisms controlling IYO nuclear accumulation were unknown, and the evidence that increased nuclear IYO levels trigger differentiation remained correlative. Searching for IYO interactors, we identified RPAP2 IYO Mate (RIMA), a homolog of yeast and human proteins linked to nuclear import of selective cargo. Knockdown of RIMA causes delayed onset of cell differentiation, phenocopying the effects of IYO knockdown at the transcriptomic and developmental levels. Moreover, differentiation is completely blocked when IYO and RIMA activities are simultaneously reduced and is synergistically accelerated when IYO and RIMA are concurrently overexpressed, confirming their functional interaction. Indeed, RIMA knockdown reduces the nuclear levels of IYO and prevents its prodifferentiation activity, supporting the conclusion that RIMA-dependent nuclear IYO accumulation triggers cell differentiation in Arabidopsis. Importantly, by analyzing the effect of the IYO/RIMA pathway on xylem pole pericycle cells, we provide compelling evidence reinforcing the view that the capacity for de novo organogenesis and regeneration from mature plant tissues can reside in stem cell reservoirs.
Fil: Muñoz, Alfonso. Consejo Superior de Investigaciones Cientificas; España
Fil: Mangano, Silvina. Consejo Superior de Investigaciones Cientificas; España. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Bioquímicas de Buenos Aires. Fundación Instituto Leloir. Instituto de Investigaciones Bioquímicas de Buenos Aires; Argentina
Fil: González-García, Mary Paz. Consejo Superior de Investigaciones Cientificas; España
Fil: Contreras, Ramón. Consejo Superior de Investigaciones Cientificas; España
Fil: Sauer, Michael. Universitat Potsdam; Alemania. Consejo Superior de Investigaciones Cientificas; España
Fil: De Rybel, Bert. University of Ghent; Bélgica. Wageningen University; Países Bajos
Fil: Weijers, Dolf. Wageningen University; Países Bajos
Fil: Sánchez Serrano, José Juan. Consejo Superior de Investigaciones Cientificas; España
Fil: Sanmartín, Maite. Consejo Superior de Investigaciones Cientificas; España
Fil: Rojo, Enrique. Consejo Superior de Investigaciones Cientificas; España
Materia
Cell Differentiation
Arabidopsis Thaliana
Organogenesis
Stem Cell
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/22868
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/22868
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling RIMA-Dependent Nuclear Accumulation of IYO Triggers Auxin-Irreversible Cell Differentiation in ArabidopsisMuñoz, AlfonsoMangano, SilvinaGonzález-García, Mary PazContreras, RamónSauer, MichaelDe Rybel, BertWeijers, DolfSánchez Serrano, José JuanSanmartín, MaiteRojo, EnriqueCell DifferentiationArabidopsis ThalianaOrganogenesisStem Cellhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1The transcriptional regulator MINIYO (IYO) is essential and rate-limiting for initiating cell differentiation in Arabidopsis thaliana Moreover, IYO moves from the cytosol into the nucleus in cells at the meristem periphery, possibly triggering their differentiation. However, the genetic mechanisms controlling IYO nuclear accumulation were unknown, and the evidence that increased nuclear IYO levels trigger differentiation remained correlative. Searching for IYO interactors, we identified RPAP2 IYO Mate (RIMA), a homolog of yeast and human proteins linked to nuclear import of selective cargo. Knockdown of RIMA causes delayed onset of cell differentiation, phenocopying the effects of IYO knockdown at the transcriptomic and developmental levels. Moreover, differentiation is completely blocked when IYO and RIMA activities are simultaneously reduced and is synergistically accelerated when IYO and RIMA are concurrently overexpressed, confirming their functional interaction. Indeed, RIMA knockdown reduces the nuclear levels of IYO and prevents its prodifferentiation activity, supporting the conclusion that RIMA-dependent nuclear IYO accumulation triggers cell differentiation in Arabidopsis. Importantly, by analyzing the effect of the IYO/RIMA pathway on xylem pole pericycle cells, we provide compelling evidence reinforcing the view that the capacity for de novo organogenesis and regeneration from mature plant tissues can reside in stem cell reservoirs.Fil: Muñoz, Alfonso. Consejo Superior de Investigaciones Cientificas; EspañaFil: Mangano, Silvina. Consejo Superior de Investigaciones Cientificas; España. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Bioquímicas de Buenos Aires. Fundación Instituto Leloir. Instituto de Investigaciones Bioquímicas de Buenos Aires; ArgentinaFil: González-García, Mary Paz. Consejo Superior de Investigaciones Cientificas; EspañaFil: Contreras, Ramón. Consejo Superior de Investigaciones Cientificas; EspañaFil: Sauer, Michael. Universitat Potsdam; Alemania. Consejo Superior de Investigaciones Cientificas; EspañaFil: De Rybel, Bert. University of Ghent; Bélgica. Wageningen University; Países BajosFil: Weijers, Dolf. Wageningen University; Países BajosFil: Sánchez Serrano, José Juan. Consejo Superior de Investigaciones Cientificas; EspañaFil: Sanmartín, Maite. Consejo Superior de Investigaciones Cientificas; EspañaFil: Rojo, Enrique. Consejo Superior de Investigaciones Cientificas; EspañaAmerican Society of Plant Biologist2017-02info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/22868Muñoz, Alfonso; Mangano, Silvina; González-García, Mary Paz; Contreras, Ramón; Sauer, Michael; et al.; RIMA-Dependent Nuclear Accumulation of IYO Triggers Auxin-Irreversible Cell Differentiation in Arabidopsis; American Society of Plant Biologist; Plant Cell; 29; 3; 2-2017; 575-5881040-46511532-298XCONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/http://www.plantcell.org/content/29/3/575.longinfo:eu-repo/semantics/altIdentifier/doi/10.1105/tpc.16.00791info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:08:56Zoai:ri.conicet.gov.ar:11336/22868instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:08:56.887CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv RIMA-Dependent Nuclear Accumulation of IYO Triggers Auxin-Irreversible Cell Differentiation in Arabidopsis
title RIMA-Dependent Nuclear Accumulation of IYO Triggers Auxin-Irreversible Cell Differentiation in Arabidopsis
spellingShingle RIMA-Dependent Nuclear Accumulation of IYO Triggers Auxin-Irreversible Cell Differentiation in Arabidopsis
Muñoz, Alfonso
Cell Differentiation
Arabidopsis Thaliana
Organogenesis
Stem Cell
title_short RIMA-Dependent Nuclear Accumulation of IYO Triggers Auxin-Irreversible Cell Differentiation in Arabidopsis
title_full RIMA-Dependent Nuclear Accumulation of IYO Triggers Auxin-Irreversible Cell Differentiation in Arabidopsis
title_fullStr RIMA-Dependent Nuclear Accumulation of IYO Triggers Auxin-Irreversible Cell Differentiation in Arabidopsis
title_full_unstemmed RIMA-Dependent Nuclear Accumulation of IYO Triggers Auxin-Irreversible Cell Differentiation in Arabidopsis
title_sort RIMA-Dependent Nuclear Accumulation of IYO Triggers Auxin-Irreversible Cell Differentiation in Arabidopsis
dc.creator.none.fl_str_mv Muñoz, Alfonso
Mangano, Silvina
González-García, Mary Paz
Contreras, Ramón
Sauer, Michael
De Rybel, Bert
Weijers, Dolf
Sánchez Serrano, José Juan
Sanmartín, Maite
Rojo, Enrique
author Muñoz, Alfonso
author_facet Muñoz, Alfonso
Mangano, Silvina
González-García, Mary Paz
Contreras, Ramón
Sauer, Michael
De Rybel, Bert
Weijers, Dolf
Sánchez Serrano, José Juan
Sanmartín, Maite
Rojo, Enrique
author_role author
author2 Mangano, Silvina
González-García, Mary Paz
Contreras, Ramón
Sauer, Michael
De Rybel, Bert
Weijers, Dolf
Sánchez Serrano, José Juan
Sanmartín, Maite
Rojo, Enrique
author2_role author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Cell Differentiation
Arabidopsis Thaliana
Organogenesis
Stem Cell
topic Cell Differentiation
Arabidopsis Thaliana
Organogenesis
Stem Cell
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv The transcriptional regulator MINIYO (IYO) is essential and rate-limiting for initiating cell differentiation in Arabidopsis thaliana Moreover, IYO moves from the cytosol into the nucleus in cells at the meristem periphery, possibly triggering their differentiation. However, the genetic mechanisms controlling IYO nuclear accumulation were unknown, and the evidence that increased nuclear IYO levels trigger differentiation remained correlative. Searching for IYO interactors, we identified RPAP2 IYO Mate (RIMA), a homolog of yeast and human proteins linked to nuclear import of selective cargo. Knockdown of RIMA causes delayed onset of cell differentiation, phenocopying the effects of IYO knockdown at the transcriptomic and developmental levels. Moreover, differentiation is completely blocked when IYO and RIMA activities are simultaneously reduced and is synergistically accelerated when IYO and RIMA are concurrently overexpressed, confirming their functional interaction. Indeed, RIMA knockdown reduces the nuclear levels of IYO and prevents its prodifferentiation activity, supporting the conclusion that RIMA-dependent nuclear IYO accumulation triggers cell differentiation in Arabidopsis. Importantly, by analyzing the effect of the IYO/RIMA pathway on xylem pole pericycle cells, we provide compelling evidence reinforcing the view that the capacity for de novo organogenesis and regeneration from mature plant tissues can reside in stem cell reservoirs.
Fil: Muñoz, Alfonso. Consejo Superior de Investigaciones Cientificas; España
Fil: Mangano, Silvina. Consejo Superior de Investigaciones Cientificas; España. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Bioquímicas de Buenos Aires. Fundación Instituto Leloir. Instituto de Investigaciones Bioquímicas de Buenos Aires; Argentina
Fil: González-García, Mary Paz. Consejo Superior de Investigaciones Cientificas; España
Fil: Contreras, Ramón. Consejo Superior de Investigaciones Cientificas; España
Fil: Sauer, Michael. Universitat Potsdam; Alemania. Consejo Superior de Investigaciones Cientificas; España
Fil: De Rybel, Bert. University of Ghent; Bélgica. Wageningen University; Países Bajos
Fil: Weijers, Dolf. Wageningen University; Países Bajos
Fil: Sánchez Serrano, José Juan. Consejo Superior de Investigaciones Cientificas; España
Fil: Sanmartín, Maite. Consejo Superior de Investigaciones Cientificas; España
Fil: Rojo, Enrique. Consejo Superior de Investigaciones Cientificas; España
description The transcriptional regulator MINIYO (IYO) is essential and rate-limiting for initiating cell differentiation in Arabidopsis thaliana Moreover, IYO moves from the cytosol into the nucleus in cells at the meristem periphery, possibly triggering their differentiation. However, the genetic mechanisms controlling IYO nuclear accumulation were unknown, and the evidence that increased nuclear IYO levels trigger differentiation remained correlative. Searching for IYO interactors, we identified RPAP2 IYO Mate (RIMA), a homolog of yeast and human proteins linked to nuclear import of selective cargo. Knockdown of RIMA causes delayed onset of cell differentiation, phenocopying the effects of IYO knockdown at the transcriptomic and developmental levels. Moreover, differentiation is completely blocked when IYO and RIMA activities are simultaneously reduced and is synergistically accelerated when IYO and RIMA are concurrently overexpressed, confirming their functional interaction. Indeed, RIMA knockdown reduces the nuclear levels of IYO and prevents its prodifferentiation activity, supporting the conclusion that RIMA-dependent nuclear IYO accumulation triggers cell differentiation in Arabidopsis. Importantly, by analyzing the effect of the IYO/RIMA pathway on xylem pole pericycle cells, we provide compelling evidence reinforcing the view that the capacity for de novo organogenesis and regeneration from mature plant tissues can reside in stem cell reservoirs.
publishDate 2017
dc.date.none.fl_str_mv 2017-02
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/22868
Muñoz, Alfonso; Mangano, Silvina; González-García, Mary Paz; Contreras, Ramón; Sauer, Michael; et al.; RIMA-Dependent Nuclear Accumulation of IYO Triggers Auxin-Irreversible Cell Differentiation in Arabidopsis; American Society of Plant Biologist; Plant Cell; 29; 3; 2-2017; 575-588
1040-4651
1532-298X
CONICET Digital
CONICET
url http://hdl.handle.net/11336/22868
identifier_str_mv Muñoz, Alfonso; Mangano, Silvina; González-García, Mary Paz; Contreras, Ramón; Sauer, Michael; et al.; RIMA-Dependent Nuclear Accumulation of IYO Triggers Auxin-Irreversible Cell Differentiation in Arabidopsis; American Society of Plant Biologist; Plant Cell; 29; 3; 2-2017; 575-588
1040-4651
1532-298X
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/http://www.plantcell.org/content/29/3/575.long
info:eu-repo/semantics/altIdentifier/doi/10.1105/tpc.16.00791
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv American Society of Plant Biologist
publisher.none.fl_str_mv American Society of Plant Biologist
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.070432

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