Bacteriocin producing lactobacilli strains as a biological strategy to control listerial growth

Autores
García, María José; Ruiz, Francisco Oscar; Asurmendi, Paula; Pascual, Liliana Myriam; Barberis, Isabel Lucila
Año de publicación
2015
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Listeria monocytogenes causes listeriosis in humans and animals, and is predominantly transmitted by ingestion of contaminated food. Its severity and high mortality rate make listeriosis a relevant foodborne disease. Currently, biological strategies using probiotic lactobacilli to prevent and control infectious diseases are being globally investigated. The biocontrol exerted by certain Lactobacillus spp. is mainly due to the production of a variety of antimicrobial substances. The aims of this work were (1) to investigate the presence of Listeria spp. in raw cow milk and (2) to determine the inhibitory activity of bacteriocin-like inhibitory substances (BLIS-es) produced by Lactobacillus fermentum L23 and Lactobacillus rhamnosus L60 on L. monocytogenes and other listerial species isolated from food and clinical samples. The presence of Listeria spp. in raw milk was evaluated in 814 samples from 238 cows. The listerial prevalence in cattle was 0.84% and the isolates were identified as L. innocua (LI1) and L. welshimeri (LW1). A total of 29 listerial strains were used as indicator microorganisms to evaluate the antimicrobial activity of BLIS-es L23 and L60. Bioactive metabolites produced by these lactobacilli strains were able to inhibit the listerial growth. This biological activity was mainly attributed to the BLIS-es L23 and L60 which, even at low concentration, were active on 100% of listerial strains. This study reveals a strong potential for the biotechnological use of these bacteriocin producing lactobacilli as a biostrategy against Listeria spp.
Fil: García, María José. Universidad Nacional de Rio Cuarto. Facultad de Cs.exactas Fisicoquimicas y Naturales. Departamento de Microbiologia E Inmunologia. Area de Bacteriologia; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba; Argentina
Fil: Ruiz, Francisco Oscar. Universidad Nacional de Rio Cuarto. Facultad de Cs.exactas Fisicoquimicas y Naturales. Departamento de Microbiologia E Inmunologia. Area de Bacteriologia; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba; Argentina
Fil: Asurmendi, Paula. Universidad Nacional de Rio Cuarto. Facultad de Cs.exactas Fisicoquimicas y Naturales. Departamento de Microbiologia E Inmunologia. Area de Bacteriologia; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba; Argentina
Fil: Pascual, Liliana Myriam. Universidad Nacional de Rio Cuarto. Facultad de Cs.exactas Fisicoquimicas y Naturales. Departamento de Microbiologia E Inmunologia. Area de Bacteriologia; Argentina
Fil: Barberis, Isabel Lucila. Universidad Nacional de Rio Cuarto. Facultad de Cs.exactas Fisicoquimicas y Naturales. Departamento de Microbiologia E Inmunologia. Area de Bacteriologia; Argentina
Materia
BACTERIOCIN
LISTERIA MONOCYTOGENES
BIOLOGICAL CONTROL
RAW MILK
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/161259
Acceder
id CONICETDig_e5d2e83f30ffe37b7b48d6c7519dcd55
oai_identifier_str oai:ri.conicet.gov.ar:11336/161259
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Bacteriocin producing lactobacilli strains as a biological strategy to control listerial growthGarcía, María JoséRuiz, Francisco OscarAsurmendi, PaulaPascual, Liliana MyriamBarberis, Isabel LucilaBACTERIOCINLISTERIA MONOCYTOGENESBIOLOGICAL CONTROLRAW MILKhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1https://purl.org/becyt/ford/3.4https://purl.org/becyt/ford/3https://purl.org/becyt/ford/4.3https://purl.org/becyt/ford/4Listeria monocytogenes causes listeriosis in humans and animals, and is predominantly transmitted by ingestion of contaminated food. Its severity and high mortality rate make listeriosis a relevant foodborne disease. Currently, biological strategies using probiotic lactobacilli to prevent and control infectious diseases are being globally investigated. The biocontrol exerted by certain Lactobacillus spp. is mainly due to the production of a variety of antimicrobial substances. The aims of this work were (1) to investigate the presence of Listeria spp. in raw cow milk and (2) to determine the inhibitory activity of bacteriocin-like inhibitory substances (BLIS-es) produced by Lactobacillus fermentum L23 and Lactobacillus rhamnosus L60 on L. monocytogenes and other listerial species isolated from food and clinical samples. The presence of Listeria spp. in raw milk was evaluated in 814 samples from 238 cows. The listerial prevalence in cattle was 0.84% and the isolates were identified as L. innocua (LI1) and L. welshimeri (LW1). A total of 29 listerial strains were used as indicator microorganisms to evaluate the antimicrobial activity of BLIS-es L23 and L60. Bioactive metabolites produced by these lactobacilli strains were able to inhibit the listerial growth. This biological activity was mainly attributed to the BLIS-es L23 and L60 which, even at low concentration, were active on 100% of listerial strains. This study reveals a strong potential for the biotechnological use of these bacteriocin producing lactobacilli as a biostrategy against Listeria spp.Fil: García, María José. Universidad Nacional de Rio Cuarto. Facultad de Cs.exactas Fisicoquimicas y Naturales. Departamento de Microbiologia E Inmunologia. Area de Bacteriologia; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba; ArgentinaFil: Ruiz, Francisco Oscar. Universidad Nacional de Rio Cuarto. Facultad de Cs.exactas Fisicoquimicas y Naturales. Departamento de Microbiologia E Inmunologia. Area de Bacteriologia; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba; ArgentinaFil: Asurmendi, Paula. Universidad Nacional de Rio Cuarto. Facultad de Cs.exactas Fisicoquimicas y Naturales. Departamento de Microbiologia E Inmunologia. Area de Bacteriologia; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba; ArgentinaFil: Pascual, Liliana Myriam. Universidad Nacional de Rio Cuarto. Facultad de Cs.exactas Fisicoquimicas y Naturales. Departamento de Microbiologia E Inmunologia. Area de Bacteriologia; ArgentinaFil: Barberis, Isabel Lucila. Universidad Nacional de Rio Cuarto. Facultad de Cs.exactas Fisicoquimicas y Naturales. Departamento de Microbiologia E Inmunologia. Area de Bacteriologia; ArgentinaSciDoc Publishers2015-09info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/161259García, María José; Ruiz, Francisco Oscar; Asurmendi, Paula; Pascual, Liliana Myriam; Barberis, Isabel Lucila; Bacteriocin producing lactobacilli strains as a biological strategy to control listerial growth; SciDoc Publishers; International Journal of Microbiology & Advanced Immunology; 9-2015; 60-642329-9967CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/http://scidoc.org/IJMAI-2329-9967-03-201.phpinfo:eu-repo/semantics/altIdentifier/doi/10.19070/2329-9967-1500011info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T10:02:07Zoai:ri.conicet.gov.ar:11336/161259instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 10:02:07.747CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Bacteriocin producing lactobacilli strains as a biological strategy to control listerial growth
title Bacteriocin producing lactobacilli strains as a biological strategy to control listerial growth
spellingShingle Bacteriocin producing lactobacilli strains as a biological strategy to control listerial growth
García, María José
BACTERIOCIN
LISTERIA MONOCYTOGENES
BIOLOGICAL CONTROL
RAW MILK
title_short Bacteriocin producing lactobacilli strains as a biological strategy to control listerial growth
title_full Bacteriocin producing lactobacilli strains as a biological strategy to control listerial growth
title_fullStr Bacteriocin producing lactobacilli strains as a biological strategy to control listerial growth
title_full_unstemmed Bacteriocin producing lactobacilli strains as a biological strategy to control listerial growth
title_sort Bacteriocin producing lactobacilli strains as a biological strategy to control listerial growth
dc.creator.none.fl_str_mv García, María José
Ruiz, Francisco Oscar
Asurmendi, Paula
Pascual, Liliana Myriam
Barberis, Isabel Lucila
author García, María José
author_facet García, María José
Ruiz, Francisco Oscar
Asurmendi, Paula
Pascual, Liliana Myriam
Barberis, Isabel Lucila
author_role author
author2 Ruiz, Francisco Oscar
Asurmendi, Paula
Pascual, Liliana Myriam
Barberis, Isabel Lucila
author2_role author
author
author
author
dc.subject.none.fl_str_mv BACTERIOCIN
LISTERIA MONOCYTOGENES
BIOLOGICAL CONTROL
RAW MILK
topic BACTERIOCIN
LISTERIA MONOCYTOGENES
BIOLOGICAL CONTROL
RAW MILK
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
https://purl.org/becyt/ford/3.4
https://purl.org/becyt/ford/3
https://purl.org/becyt/ford/4.3
https://purl.org/becyt/ford/4
dc.description.none.fl_txt_mv Listeria monocytogenes causes listeriosis in humans and animals, and is predominantly transmitted by ingestion of contaminated food. Its severity and high mortality rate make listeriosis a relevant foodborne disease. Currently, biological strategies using probiotic lactobacilli to prevent and control infectious diseases are being globally investigated. The biocontrol exerted by certain Lactobacillus spp. is mainly due to the production of a variety of antimicrobial substances. The aims of this work were (1) to investigate the presence of Listeria spp. in raw cow milk and (2) to determine the inhibitory activity of bacteriocin-like inhibitory substances (BLIS-es) produced by Lactobacillus fermentum L23 and Lactobacillus rhamnosus L60 on L. monocytogenes and other listerial species isolated from food and clinical samples. The presence of Listeria spp. in raw milk was evaluated in 814 samples from 238 cows. The listerial prevalence in cattle was 0.84% and the isolates were identified as L. innocua (LI1) and L. welshimeri (LW1). A total of 29 listerial strains were used as indicator microorganisms to evaluate the antimicrobial activity of BLIS-es L23 and L60. Bioactive metabolites produced by these lactobacilli strains were able to inhibit the listerial growth. This biological activity was mainly attributed to the BLIS-es L23 and L60 which, even at low concentration, were active on 100% of listerial strains. This study reveals a strong potential for the biotechnological use of these bacteriocin producing lactobacilli as a biostrategy against Listeria spp.
Fil: García, María José. Universidad Nacional de Rio Cuarto. Facultad de Cs.exactas Fisicoquimicas y Naturales. Departamento de Microbiologia E Inmunologia. Area de Bacteriologia; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba; Argentina
Fil: Ruiz, Francisco Oscar. Universidad Nacional de Rio Cuarto. Facultad de Cs.exactas Fisicoquimicas y Naturales. Departamento de Microbiologia E Inmunologia. Area de Bacteriologia; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba; Argentina
Fil: Asurmendi, Paula. Universidad Nacional de Rio Cuarto. Facultad de Cs.exactas Fisicoquimicas y Naturales. Departamento de Microbiologia E Inmunologia. Area de Bacteriologia; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba; Argentina
Fil: Pascual, Liliana Myriam. Universidad Nacional de Rio Cuarto. Facultad de Cs.exactas Fisicoquimicas y Naturales. Departamento de Microbiologia E Inmunologia. Area de Bacteriologia; Argentina
Fil: Barberis, Isabel Lucila. Universidad Nacional de Rio Cuarto. Facultad de Cs.exactas Fisicoquimicas y Naturales. Departamento de Microbiologia E Inmunologia. Area de Bacteriologia; Argentina
description Listeria monocytogenes causes listeriosis in humans and animals, and is predominantly transmitted by ingestion of contaminated food. Its severity and high mortality rate make listeriosis a relevant foodborne disease. Currently, biological strategies using probiotic lactobacilli to prevent and control infectious diseases are being globally investigated. The biocontrol exerted by certain Lactobacillus spp. is mainly due to the production of a variety of antimicrobial substances. The aims of this work were (1) to investigate the presence of Listeria spp. in raw cow milk and (2) to determine the inhibitory activity of bacteriocin-like inhibitory substances (BLIS-es) produced by Lactobacillus fermentum L23 and Lactobacillus rhamnosus L60 on L. monocytogenes and other listerial species isolated from food and clinical samples. The presence of Listeria spp. in raw milk was evaluated in 814 samples from 238 cows. The listerial prevalence in cattle was 0.84% and the isolates were identified as L. innocua (LI1) and L. welshimeri (LW1). A total of 29 listerial strains were used as indicator microorganisms to evaluate the antimicrobial activity of BLIS-es L23 and L60. Bioactive metabolites produced by these lactobacilli strains were able to inhibit the listerial growth. This biological activity was mainly attributed to the BLIS-es L23 and L60 which, even at low concentration, were active on 100% of listerial strains. This study reveals a strong potential for the biotechnological use of these bacteriocin producing lactobacilli as a biostrategy against Listeria spp.
publishDate 2015
dc.date.none.fl_str_mv 2015-09
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/161259
García, María José; Ruiz, Francisco Oscar; Asurmendi, Paula; Pascual, Liliana Myriam; Barberis, Isabel Lucila; Bacteriocin producing lactobacilli strains as a biological strategy to control listerial growth; SciDoc Publishers; International Journal of Microbiology & Advanced Immunology; 9-2015; 60-64
2329-9967
CONICET Digital
CONICET
url http://hdl.handle.net/11336/161259
identifier_str_mv García, María José; Ruiz, Francisco Oscar; Asurmendi, Paula; Pascual, Liliana Myriam; Barberis, Isabel Lucila; Bacteriocin producing lactobacilli strains as a biological strategy to control listerial growth; SciDoc Publishers; International Journal of Microbiology & Advanced Immunology; 9-2015; 60-64
2329-9967
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/http://scidoc.org/IJMAI-2329-9967-03-201.php
info:eu-repo/semantics/altIdentifier/doi/10.19070/2329-9967-1500011
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv SciDoc Publishers
publisher.none.fl_str_mv SciDoc Publishers
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.13397

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