Efects of orexin a and b on the expression of its receptors ox1 and ox2 in superovulated rat ovarian cells

Autores
Cataldi, Natalia Isabel; Lux, Victoria Adela R.; Libertun, Carlos
Año de publicación
2010
Idioma
inglés
Tipo de recurso
documento de conferencia
Estado
versión publicada
Descripción
In previous works we described changes in the orexinergic hypo thalamus-pituitary-ovarian system in adult rats (AJP-EM 292: E820- E828, 2007; AJP-EM, 293: E977-85. 2007). Here we continue to study in vitro in ovaries of superovulated prepubertal rats (SPO) the presence of OX1 and OX2 receptors and the effects of orexin A (OXA) and B (OXB) on their expression. SPO rat ovarian cells were cultured and stimulated for 48 h with OXA (10-9M) and OXB (10-9M) in the presence or absence of their specific antagonists. We evaluated the secretion of progesterone released into the me dium by RIA. The quantification of the receptors was determined by real-time RT-PCR as in AJP-EM 293: E977-85. 2007. [...]
Fil: Cataldi, Natalia Isabel. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; Argentina
Fil: Lux, Victoria Adela R.. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; Argentina
Fil: Libertun, Carlos. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; Argentina. Universidad de Buenos Aires; Argentina
Eleventh Multidisciplinary Workshop
Buenos Aires
Argentina
Sociedad Argentina de Biología
Materia
OREXIN
OVARY
RECEPTOR
CULTIVO
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/200033
Acceder
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spelling Efects of orexin a and b on the expression of its receptors ox1 and ox2 in superovulated rat ovarian cellsCataldi, Natalia IsabelLux, Victoria Adela R.Libertun, CarlosOREXINOVARYRECEPTORCULTIVOhttps://purl.org/becyt/ford/3.1https://purl.org/becyt/ford/3In previous works we described changes in the orexinergic hypo thalamus-pituitary-ovarian system in adult rats (AJP-EM 292: E820- E828, 2007; AJP-EM, 293: E977-85. 2007). Here we continue to study in vitro in ovaries of superovulated prepubertal rats (SPO) the presence of OX1 and OX2 receptors and the effects of orexin A (OXA) and B (OXB) on their expression. SPO rat ovarian cells were cultured and stimulated for 48 h with OXA (10-9M) and OXB (10-9M) in the presence or absence of their specific antagonists. We evaluated the secretion of progesterone released into the me dium by RIA. The quantification of the receptors was determined by real-time RT-PCR as in AJP-EM 293: E977-85. 2007. [...]Fil: Cataldi, Natalia Isabel. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; ArgentinaFil: Lux, Victoria Adela R.. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; ArgentinaFil: Libertun, Carlos. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; Argentina. Universidad de Buenos Aires; ArgentinaEleventh Multidisciplinary WorkshopBuenos AiresArgentinaSociedad Argentina de BiologíaTech Science Press2010info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/conferenceObjectWorkshopJournalhttp://purl.org/coar/resource_type/c_5794info:ar-repo/semantics/documentoDeConferenciaapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/200033Efects of orexin a and b on the expression of its receptors ox1 and ox2 in superovulated rat ovarian cells; Eleventh Multidisciplinary Workshop; Buenos Aires; Argentina; 2009; 91-910327-9545CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.techscience.com/biocell/v34nSuppl.S/37994Nacionalinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-11-26T09:07:23Zoai:ri.conicet.gov.ar:11336/200033instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-11-26 09:07:23.489CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Efects of orexin a and b on the expression of its receptors ox1 and ox2 in superovulated rat ovarian cells
title Efects of orexin a and b on the expression of its receptors ox1 and ox2 in superovulated rat ovarian cells
spellingShingle Efects of orexin a and b on the expression of its receptors ox1 and ox2 in superovulated rat ovarian cells
Cataldi, Natalia Isabel
OREXIN
OVARY
RECEPTOR
CULTIVO
title_short Efects of orexin a and b on the expression of its receptors ox1 and ox2 in superovulated rat ovarian cells
title_full Efects of orexin a and b on the expression of its receptors ox1 and ox2 in superovulated rat ovarian cells
title_fullStr Efects of orexin a and b on the expression of its receptors ox1 and ox2 in superovulated rat ovarian cells
title_full_unstemmed Efects of orexin a and b on the expression of its receptors ox1 and ox2 in superovulated rat ovarian cells
title_sort Efects of orexin a and b on the expression of its receptors ox1 and ox2 in superovulated rat ovarian cells
dc.creator.none.fl_str_mv Cataldi, Natalia Isabel
Lux, Victoria Adela R.
Libertun, Carlos
author Cataldi, Natalia Isabel
author_facet Cataldi, Natalia Isabel
Lux, Victoria Adela R.
Libertun, Carlos
author_role author
author2 Lux, Victoria Adela R.
Libertun, Carlos
author2_role author
author
dc.subject.none.fl_str_mv OREXIN
OVARY
RECEPTOR
CULTIVO
topic OREXIN
OVARY
RECEPTOR
CULTIVO
purl_subject.fl_str_mv https://purl.org/becyt/ford/3.1
https://purl.org/becyt/ford/3
dc.description.none.fl_txt_mv In previous works we described changes in the orexinergic hypo thalamus-pituitary-ovarian system in adult rats (AJP-EM 292: E820- E828, 2007; AJP-EM, 293: E977-85. 2007). Here we continue to study in vitro in ovaries of superovulated prepubertal rats (SPO) the presence of OX1 and OX2 receptors and the effects of orexin A (OXA) and B (OXB) on their expression. SPO rat ovarian cells were cultured and stimulated for 48 h with OXA (10-9M) and OXB (10-9M) in the presence or absence of their specific antagonists. We evaluated the secretion of progesterone released into the me dium by RIA. The quantification of the receptors was determined by real-time RT-PCR as in AJP-EM 293: E977-85. 2007. [...]
Fil: Cataldi, Natalia Isabel. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; Argentina
Fil: Lux, Victoria Adela R.. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; Argentina
Fil: Libertun, Carlos. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; Argentina. Universidad de Buenos Aires; Argentina
Eleventh Multidisciplinary Workshop
Buenos Aires
Argentina
Sociedad Argentina de Biología
description In previous works we described changes in the orexinergic hypo thalamus-pituitary-ovarian system in adult rats (AJP-EM 292: E820- E828, 2007; AJP-EM, 293: E977-85. 2007). Here we continue to study in vitro in ovaries of superovulated prepubertal rats (SPO) the presence of OX1 and OX2 receptors and the effects of orexin A (OXA) and B (OXB) on their expression. SPO rat ovarian cells were cultured and stimulated for 48 h with OXA (10-9M) and OXB (10-9M) in the presence or absence of their specific antagonists. We evaluated the secretion of progesterone released into the me dium by RIA. The quantification of the receptors was determined by real-time RT-PCR as in AJP-EM 293: E977-85. 2007. [...]
publishDate 2010
dc.date.none.fl_str_mv 2010
dc.type.none.fl_str_mv info:eu-repo/semantics/publishedVersion
info:eu-repo/semantics/conferenceObject
Workshop
Journal
http://purl.org/coar/resource_type/c_5794
info:ar-repo/semantics/documentoDeConferencia
status_str publishedVersion
format conferenceObject
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/200033
Efects of orexin a and b on the expression of its receptors ox1 and ox2 in superovulated rat ovarian cells; Eleventh Multidisciplinary Workshop; Buenos Aires; Argentina; 2009; 91-91
0327-9545
CONICET Digital
CONICET
url http://hdl.handle.net/11336/200033
identifier_str_mv Efects of orexin a and b on the expression of its receptors ox1 and ox2 in superovulated rat ovarian cells; Eleventh Multidisciplinary Workshop; Buenos Aires; Argentina; 2009; 91-91
0327-9545
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://www.techscience.com/biocell/v34nSuppl.S/37994
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rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.coverage.none.fl_str_mv Nacional
dc.publisher.none.fl_str_mv Tech Science Press
publisher.none.fl_str_mv Tech Science Press
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
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repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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