Characterization of Trypanosoma cruzi l-cysteine transport mechanisms and their adaptive regulation

Autores
Canepa, Gaspar Exequiel; Bouvier, León Alberto; Miranda, Mariana Reneé; Uttaro, Antonio Domingo; Pereira, Claudio Alejandro
Año de publicación
2009
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
L-Cysteine and methionine are unique amino acids that act as sulfur donors in all organisms. In the specific case of Trypanosomatids, L-cysteine is particularly relevant as a substrate in the synthesis of trypanothione. Although it can be synthesized de novo, L-cysteine is actively transported in Trypanosoma cruzi epimastigote cells. L-Cysteine uptake is highly specific; none of the amino acids assayed yield significant differences in terms of transport rates. L-Cysteine is transported by epimastigote cells with a calculated apparent Km of 49.5 μM and a Vmax of about 13 pmol min−1 per 107 cells. This transport is finely regulated by amino acid starvation, extracellular pH, and between the parasite growth phases. In addition, L-cysteine is incorporated post-translationally into proteins, suggesting its role in iron–sulfur core formation. Finally, the metabolic fates of L-cysteine were predicted in silico.
Fil: Canepa, Gaspar Exequiel. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Investigaciones Médicas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Bouvier, León Alberto. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Investigaciones Médicas; Argentina
Fil: Miranda, Mariana Reneé. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Investigaciones Médicas; Argentina
Fil: Uttaro, Antonio Domingo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Instituto de Biología Molecular y Celular de Rosario. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Biología Molecular y Celular de Rosario; Argentina
Fil: Pereira, Claudio Alejandro. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Investigaciones Médicas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Materia
Trypanosoma cruzi
metabolite transport
L-cysteine
amino acid transporter
Chagas disease
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/103590
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/103590
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Characterization of Trypanosoma cruzi l-cysteine transport mechanisms and their adaptive regulationCanepa, Gaspar ExequielBouvier, León AlbertoMiranda, Mariana ReneéUttaro, Antonio DomingoPereira, Claudio AlejandroTrypanosoma cruzimetabolite transportL-cysteineamino acid transporterChagas diseasehttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1L-Cysteine and methionine are unique amino acids that act as sulfur donors in all organisms. In the specific case of Trypanosomatids, L-cysteine is particularly relevant as a substrate in the synthesis of trypanothione. Although it can be synthesized de novo, L-cysteine is actively transported in Trypanosoma cruzi epimastigote cells. L-Cysteine uptake is highly specific; none of the amino acids assayed yield significant differences in terms of transport rates. L-Cysteine is transported by epimastigote cells with a calculated apparent Km of 49.5 μM and a Vmax of about 13 pmol min−1 per 107 cells. This transport is finely regulated by amino acid starvation, extracellular pH, and between the parasite growth phases. In addition, L-cysteine is incorporated post-translationally into proteins, suggesting its role in iron–sulfur core formation. Finally, the metabolic fates of L-cysteine were predicted in silico.Fil: Canepa, Gaspar Exequiel. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Investigaciones Médicas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Bouvier, León Alberto. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Investigaciones Médicas; ArgentinaFil: Miranda, Mariana Reneé. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Investigaciones Médicas; ArgentinaFil: Uttaro, Antonio Domingo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Instituto de Biología Molecular y Celular de Rosario. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Biología Molecular y Celular de Rosario; ArgentinaFil: Pereira, Claudio Alejandro. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Investigaciones Médicas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaWiley Blackwell Publishing, Inc2009-03info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/103590Canepa, Gaspar Exequiel; Bouvier, León Alberto; Miranda, Mariana Reneé; Uttaro, Antonio Domingo; Pereira, Claudio Alejandro; Characterization of Trypanosoma cruzi l-cysteine transport mechanisms and their adaptive regulation; Wiley Blackwell Publishing, Inc; FEMS Microbiology Letters; 292; 1; 3-2009; 27-320378-1097CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1111/j.1574-6968.2008.01467.xinfo:eu-repo/semantics/altIdentifier/url/https://academic.oup.com/femsle/article/292/1/27/490065info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-22T11:46:30Zoai:ri.conicet.gov.ar:11336/103590instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-22 11:46:30.827CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Characterization of Trypanosoma cruzi l-cysteine transport mechanisms and their adaptive regulation
title Characterization of Trypanosoma cruzi l-cysteine transport mechanisms and their adaptive regulation
spellingShingle Characterization of Trypanosoma cruzi l-cysteine transport mechanisms and their adaptive regulation
Canepa, Gaspar Exequiel
Trypanosoma cruzi
metabolite transport
L-cysteine
amino acid transporter
Chagas disease
title_short Characterization of Trypanosoma cruzi l-cysteine transport mechanisms and their adaptive regulation
title_full Characterization of Trypanosoma cruzi l-cysteine transport mechanisms and their adaptive regulation
title_fullStr Characterization of Trypanosoma cruzi l-cysteine transport mechanisms and their adaptive regulation
title_full_unstemmed Characterization of Trypanosoma cruzi l-cysteine transport mechanisms and their adaptive regulation
title_sort Characterization of Trypanosoma cruzi l-cysteine transport mechanisms and their adaptive regulation
dc.creator.none.fl_str_mv Canepa, Gaspar Exequiel
Bouvier, León Alberto
Miranda, Mariana Reneé
Uttaro, Antonio Domingo
Pereira, Claudio Alejandro
author Canepa, Gaspar Exequiel
author_facet Canepa, Gaspar Exequiel
Bouvier, León Alberto
Miranda, Mariana Reneé
Uttaro, Antonio Domingo
Pereira, Claudio Alejandro
author_role author
author2 Bouvier, León Alberto
Miranda, Mariana Reneé
Uttaro, Antonio Domingo
Pereira, Claudio Alejandro
author2_role author
author
author
author
dc.subject.none.fl_str_mv Trypanosoma cruzi
metabolite transport
L-cysteine
amino acid transporter
Chagas disease
topic Trypanosoma cruzi
metabolite transport
L-cysteine
amino acid transporter
Chagas disease
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv L-Cysteine and methionine are unique amino acids that act as sulfur donors in all organisms. In the specific case of Trypanosomatids, L-cysteine is particularly relevant as a substrate in the synthesis of trypanothione. Although it can be synthesized de novo, L-cysteine is actively transported in Trypanosoma cruzi epimastigote cells. L-Cysteine uptake is highly specific; none of the amino acids assayed yield significant differences in terms of transport rates. L-Cysteine is transported by epimastigote cells with a calculated apparent Km of 49.5 μM and a Vmax of about 13 pmol min−1 per 107 cells. This transport is finely regulated by amino acid starvation, extracellular pH, and between the parasite growth phases. In addition, L-cysteine is incorporated post-translationally into proteins, suggesting its role in iron–sulfur core formation. Finally, the metabolic fates of L-cysteine were predicted in silico.
Fil: Canepa, Gaspar Exequiel. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Investigaciones Médicas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Bouvier, León Alberto. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Investigaciones Médicas; Argentina
Fil: Miranda, Mariana Reneé. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Investigaciones Médicas; Argentina
Fil: Uttaro, Antonio Domingo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Instituto de Biología Molecular y Celular de Rosario. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Biología Molecular y Celular de Rosario; Argentina
Fil: Pereira, Claudio Alejandro. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Investigaciones Médicas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
description L-Cysteine and methionine are unique amino acids that act as sulfur donors in all organisms. In the specific case of Trypanosomatids, L-cysteine is particularly relevant as a substrate in the synthesis of trypanothione. Although it can be synthesized de novo, L-cysteine is actively transported in Trypanosoma cruzi epimastigote cells. L-Cysteine uptake is highly specific; none of the amino acids assayed yield significant differences in terms of transport rates. L-Cysteine is transported by epimastigote cells with a calculated apparent Km of 49.5 μM and a Vmax of about 13 pmol min−1 per 107 cells. This transport is finely regulated by amino acid starvation, extracellular pH, and between the parasite growth phases. In addition, L-cysteine is incorporated post-translationally into proteins, suggesting its role in iron–sulfur core formation. Finally, the metabolic fates of L-cysteine were predicted in silico.
publishDate 2009
dc.date.none.fl_str_mv 2009-03
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/103590
Canepa, Gaspar Exequiel; Bouvier, León Alberto; Miranda, Mariana Reneé; Uttaro, Antonio Domingo; Pereira, Claudio Alejandro; Characterization of Trypanosoma cruzi l-cysteine transport mechanisms and their adaptive regulation; Wiley Blackwell Publishing, Inc; FEMS Microbiology Letters; 292; 1; 3-2009; 27-32
0378-1097
CONICET Digital
CONICET
url http://hdl.handle.net/11336/103590
identifier_str_mv Canepa, Gaspar Exequiel; Bouvier, León Alberto; Miranda, Mariana Reneé; Uttaro, Antonio Domingo; Pereira, Claudio Alejandro; Characterization of Trypanosoma cruzi l-cysteine transport mechanisms and their adaptive regulation; Wiley Blackwell Publishing, Inc; FEMS Microbiology Letters; 292; 1; 3-2009; 27-32
0378-1097
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1111/j.1574-6968.2008.01467.x
info:eu-repo/semantics/altIdentifier/url/https://academic.oup.com/femsle/article/292/1/27/490065
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Wiley Blackwell Publishing, Inc
publisher.none.fl_str_mv Wiley Blackwell Publishing, Inc
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 12.982451

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