Innate immune response in Neospora caninum and BoAHV-1 coinfection: The interaction of TLRs and cathelicidins
- Autores
- Plá, Natalia; Burucúa, Mercedes María; Moore, Dadin Prando; Odeón, Anselmo Carlos; Cobo, Eduardo Ruben; Quintana, Silvina; Marin, Maia Solange
- Año de publicación
- 2024
- Idioma
- inglés
- Tipo de recurso
- documento de conferencia
- Estado
- versión publicada
- Descripción
- Neospora caninum causes significant global economic losses due to abortions in cattle. Immunosuppression induced by viral infection worsens neosporosis. However, the innate immune mechanisms in N. caninum and BoAHV-1 co-infections remain unknown. We previouslyshowed an overexpression of toll-like receptor (TLR) 7 and LL-37 human cathelicidin in this co-infection. This study investigated in vitro effects of bovine alpha-herpesvirus-1 (BoAHV-1) infection on TLRs and cathelicidin antimicrobial peptides signaling, and N. caninum replication in co-infected neuronal cells. Human neuroblastoma SH-SY5Y cells were pretreated with chloroquine (inhibitor of endosomal TLRs, 10 µg/ml), imiquimod (agonist of TLR7, 5 µg/ml),sodium butyrate (promoter of endogenous cathelicidin, 4 mM), or subjected to silencing of LL-37 (shLL-37), followed by infection with the reference strains N. caninum NC-1 (MOI: 0.2) and BoAHV-1 Cooper (MOI: 0.1). At 24 h post-infection, gene expression levels of key immune molecules involved in TLR mediated signaling pathways (NF-κβ, MyD88, IRF3, and IRF7) were assessed by RT-qPCR and tachyzoite replication was examined microscopically. The results indicated that N. caninum replication in coinfection with BoAHV-1 was significantly decreased both intracellularly and extracellularly after chloroquine pretreatment and shLL-37 compared to imiquimod and sodium butyrate treatments. This reduction was accompanied in co-infection by a significant increase, with respect to the control, in MyD88 and IRF3 expression (3.9-fold and 5.5- fold, respectively, p<0.05) after chloroquine treatment, and a decrease in IRF3 expression after shLL-37 (0.409-fold, p<0.05). While treatments with imiquimod and sodium butyrate resulted in a significant decrease, with respect to the control, in the expression of three of the four molecules analyzed (NF-κβ: 0.126 and 0.074; MyD88: 0.365 and 0.508; IRF7: 0.078 and 0.01, respectively, p<0.05). These findings highlight the role of TLR7 and LL-37 in co-infection, emphasizing their impact on dynamics. TLR7 and LL-37 regulate BoAHV- 1 replication, promoting N. caninum replication when both pathogens are present.
Fil: Plá, Natalia. Universidad Nacional de Mar del Plata. Facultad de Ciencias Agrarias; Argentina
Fil: Burucúa, Mercedes María. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales. Departamento de Biología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Innovación para la Producción Agropecuaria y el Desarrollo Sostenible - Instituto Nacional de Tecnología Agropecuaria. Centro Regional Buenos Aires Sur. Estación Experimental Agropecuaria Balcarce. Instituto de Innovación para la Producción Agropecuaria y el Desarrollo Sostenible; Argentina
Fil: Moore, Dadin Prando. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Innovación para la Producción Agropecuaria y el Desarrollo Sostenible - Instituto Nacional de Tecnología Agropecuaria. Centro Regional Buenos Aires Sur. Estación Experimental Agropecuaria Balcarce. Instituto de Innovación para la Producción Agropecuaria y el Desarrollo Sostenible; Argentina. Universidad Nacional de Mar del Plata. Facultad de Cs.agrarias. Departamento de Producción Animal; Argentina
Fil: Odeón, Anselmo Carlos. Universidad Nacional de Mar del Plata. Facultad de Cs.agrarias. Departamento de Producción Animal; Argentina
Fil: Cobo, Eduardo Ruben. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Quintana, Silvina. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales. Instituto de Investigaciones en Sanidad Producción y Ambiente. - Comisión de Investigaciones Científicas de la Provincia de Buenos Aires. Instituto de Investigaciones en Sanidad Producción y Ambiente; Argentina
Fil: Marin, Maia Solange. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Innovación para la Producción Agropecuaria y el Desarrollo Sostenible - Instituto Nacional de Tecnología Agropecuaria. Centro Regional Buenos Aires Sur. Estación Experimental Agropecuaria Balcarce. Instituto de Innovación para la Producción Agropecuaria y el Desarrollo Sostenible; Argentina. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales. Departamento de Biología; Argentina
VII International Meeting of Apicomplexan parasites in farm animals - ApiCOWplexa
La Plata
Argentina
Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias - Materia
-
NEOSPORA CANINUM
BOVINE ALPHAHERPESVIRUS
CATHELICIDINS
TOLL-LIKE RECEPTOS - Nivel de accesibilidad
- acceso abierto
- Condiciones de uso
- https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
- Repositorio
.jpg)
- Institución
- Consejo Nacional de Investigaciones Científicas y Técnicas
- OAI Identificador
- oai:ri.conicet.gov.ar:11336/275682
Ver los metadatos del registro completo
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Innate immune response in Neospora caninum and BoAHV-1 coinfection: The interaction of TLRs and cathelicidinsPlá, NataliaBurucúa, Mercedes MaríaMoore, Dadin PrandoOdeón, Anselmo CarlosCobo, Eduardo RubenQuintana, SilvinaMarin, Maia SolangeNEOSPORA CANINUMBOVINE ALPHAHERPESVIRUSCATHELICIDINSTOLL-LIKE RECEPTOShttps://purl.org/becyt/ford/4.3https://purl.org/becyt/ford/4Neospora caninum causes significant global economic losses due to abortions in cattle. Immunosuppression induced by viral infection worsens neosporosis. However, the innate immune mechanisms in N. caninum and BoAHV-1 co-infections remain unknown. We previouslyshowed an overexpression of toll-like receptor (TLR) 7 and LL-37 human cathelicidin in this co-infection. This study investigated in vitro effects of bovine alpha-herpesvirus-1 (BoAHV-1) infection on TLRs and cathelicidin antimicrobial peptides signaling, and N. caninum replication in co-infected neuronal cells. Human neuroblastoma SH-SY5Y cells were pretreated with chloroquine (inhibitor of endosomal TLRs, 10 µg/ml), imiquimod (agonist of TLR7, 5 µg/ml),sodium butyrate (promoter of endogenous cathelicidin, 4 mM), or subjected to silencing of LL-37 (shLL-37), followed by infection with the reference strains N. caninum NC-1 (MOI: 0.2) and BoAHV-1 Cooper (MOI: 0.1). At 24 h post-infection, gene expression levels of key immune molecules involved in TLR mediated signaling pathways (NF-κβ, MyD88, IRF3, and IRF7) were assessed by RT-qPCR and tachyzoite replication was examined microscopically. The results indicated that N. caninum replication in coinfection with BoAHV-1 was significantly decreased both intracellularly and extracellularly after chloroquine pretreatment and shLL-37 compared to imiquimod and sodium butyrate treatments. This reduction was accompanied in co-infection by a significant increase, with respect to the control, in MyD88 and IRF3 expression (3.9-fold and 5.5- fold, respectively, p<0.05) after chloroquine treatment, and a decrease in IRF3 expression after shLL-37 (0.409-fold, p<0.05). While treatments with imiquimod and sodium butyrate resulted in a significant decrease, with respect to the control, in the expression of three of the four molecules analyzed (NF-κβ: 0.126 and 0.074; MyD88: 0.365 and 0.508; IRF7: 0.078 and 0.01, respectively, p<0.05). These findings highlight the role of TLR7 and LL-37 in co-infection, emphasizing their impact on dynamics. TLR7 and LL-37 regulate BoAHV- 1 replication, promoting N. caninum replication when both pathogens are present.Fil: Plá, Natalia. Universidad Nacional de Mar del Plata. Facultad de Ciencias Agrarias; ArgentinaFil: Burucúa, Mercedes María. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales. Departamento de Biología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Innovación para la Producción Agropecuaria y el Desarrollo Sostenible - Instituto Nacional de Tecnología Agropecuaria. Centro Regional Buenos Aires Sur. Estación Experimental Agropecuaria Balcarce. Instituto de Innovación para la Producción Agropecuaria y el Desarrollo Sostenible; ArgentinaFil: Moore, Dadin Prando. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Innovación para la Producción Agropecuaria y el Desarrollo Sostenible - Instituto Nacional de Tecnología Agropecuaria. Centro Regional Buenos Aires Sur. Estación Experimental Agropecuaria Balcarce. Instituto de Innovación para la Producción Agropecuaria y el Desarrollo Sostenible; Argentina. Universidad Nacional de Mar del Plata. Facultad de Cs.agrarias. Departamento de Producción Animal; ArgentinaFil: Odeón, Anselmo Carlos. Universidad Nacional de Mar del Plata. Facultad de Cs.agrarias. Departamento de Producción Animal; ArgentinaFil: Cobo, Eduardo Ruben. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Quintana, Silvina. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales. Instituto de Investigaciones en Sanidad Producción y Ambiente. - Comisión de Investigaciones Científicas de la Provincia de Buenos Aires. Instituto de Investigaciones en Sanidad Producción y Ambiente; ArgentinaFil: Marin, Maia Solange. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Innovación para la Producción Agropecuaria y el Desarrollo Sostenible - Instituto Nacional de Tecnología Agropecuaria. Centro Regional Buenos Aires Sur. Estación Experimental Agropecuaria Balcarce. Instituto de Innovación para la Producción Agropecuaria y el Desarrollo Sostenible; Argentina. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales. Departamento de Biología; ArgentinaVII International Meeting of Apicomplexan parasites in farm animals - ApiCOWplexaLa PlataArgentinaUniversidad Nacional de La Plata. Facultad de Ciencias VeterinariasUniversidad Nacional de La Plata. Facultad de Ciencias Veterinarias2024info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/conferenceObjectEncuentroBookhttp://purl.org/coar/resource_type/c_5794info:ar-repo/semantics/documentoDeConferenciaapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/275682Innate immune response in Neospora caninum and BoAHV-1 coinfection: The interaction of TLRs and cathelicidins; VII International Meeting of Apicomplexan parasites in farm animals - ApiCOWplexa; La Plata; Argentina; 2024; 121-122978-950-34-2444-5CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://congresos.unlp.edu.ar/apicowplexa2024/info:eu-repo/semantics/altIdentifier/url/https://sedici.unlp.edu.ar/handle/10915/174543Internacionalinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-12-17T14:06:22Zoai:ri.conicet.gov.ar:11336/275682instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-12-17 14:06:22.849CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse |
| dc.title.none.fl_str_mv |
Innate immune response in Neospora caninum and BoAHV-1 coinfection: The interaction of TLRs and cathelicidins |
| title |
Innate immune response in Neospora caninum and BoAHV-1 coinfection: The interaction of TLRs and cathelicidins |
| spellingShingle |
Innate immune response in Neospora caninum and BoAHV-1 coinfection: The interaction of TLRs and cathelicidins Plá, Natalia NEOSPORA CANINUM BOVINE ALPHAHERPESVIRUS CATHELICIDINS TOLL-LIKE RECEPTOS |
| title_short |
Innate immune response in Neospora caninum and BoAHV-1 coinfection: The interaction of TLRs and cathelicidins |
| title_full |
Innate immune response in Neospora caninum and BoAHV-1 coinfection: The interaction of TLRs and cathelicidins |
| title_fullStr |
Innate immune response in Neospora caninum and BoAHV-1 coinfection: The interaction of TLRs and cathelicidins |
| title_full_unstemmed |
Innate immune response in Neospora caninum and BoAHV-1 coinfection: The interaction of TLRs and cathelicidins |
| title_sort |
Innate immune response in Neospora caninum and BoAHV-1 coinfection: The interaction of TLRs and cathelicidins |
| dc.creator.none.fl_str_mv |
Plá, Natalia Burucúa, Mercedes María Moore, Dadin Prando Odeón, Anselmo Carlos Cobo, Eduardo Ruben Quintana, Silvina Marin, Maia Solange |
| author |
Plá, Natalia |
| author_facet |
Plá, Natalia Burucúa, Mercedes María Moore, Dadin Prando Odeón, Anselmo Carlos Cobo, Eduardo Ruben Quintana, Silvina Marin, Maia Solange |
| author_role |
author |
| author2 |
Burucúa, Mercedes María Moore, Dadin Prando Odeón, Anselmo Carlos Cobo, Eduardo Ruben Quintana, Silvina Marin, Maia Solange |
| author2_role |
author author author author author author |
| dc.subject.none.fl_str_mv |
NEOSPORA CANINUM BOVINE ALPHAHERPESVIRUS CATHELICIDINS TOLL-LIKE RECEPTOS |
| topic |
NEOSPORA CANINUM BOVINE ALPHAHERPESVIRUS CATHELICIDINS TOLL-LIKE RECEPTOS |
| purl_subject.fl_str_mv |
https://purl.org/becyt/ford/4.3 https://purl.org/becyt/ford/4 |
| dc.description.none.fl_txt_mv |
Neospora caninum causes significant global economic losses due to abortions in cattle. Immunosuppression induced by viral infection worsens neosporosis. However, the innate immune mechanisms in N. caninum and BoAHV-1 co-infections remain unknown. We previouslyshowed an overexpression of toll-like receptor (TLR) 7 and LL-37 human cathelicidin in this co-infection. This study investigated in vitro effects of bovine alpha-herpesvirus-1 (BoAHV-1) infection on TLRs and cathelicidin antimicrobial peptides signaling, and N. caninum replication in co-infected neuronal cells. Human neuroblastoma SH-SY5Y cells were pretreated with chloroquine (inhibitor of endosomal TLRs, 10 µg/ml), imiquimod (agonist of TLR7, 5 µg/ml),sodium butyrate (promoter of endogenous cathelicidin, 4 mM), or subjected to silencing of LL-37 (shLL-37), followed by infection with the reference strains N. caninum NC-1 (MOI: 0.2) and BoAHV-1 Cooper (MOI: 0.1). At 24 h post-infection, gene expression levels of key immune molecules involved in TLR mediated signaling pathways (NF-κβ, MyD88, IRF3, and IRF7) were assessed by RT-qPCR and tachyzoite replication was examined microscopically. The results indicated that N. caninum replication in coinfection with BoAHV-1 was significantly decreased both intracellularly and extracellularly after chloroquine pretreatment and shLL-37 compared to imiquimod and sodium butyrate treatments. This reduction was accompanied in co-infection by a significant increase, with respect to the control, in MyD88 and IRF3 expression (3.9-fold and 5.5- fold, respectively, p<0.05) after chloroquine treatment, and a decrease in IRF3 expression after shLL-37 (0.409-fold, p<0.05). While treatments with imiquimod and sodium butyrate resulted in a significant decrease, with respect to the control, in the expression of three of the four molecules analyzed (NF-κβ: 0.126 and 0.074; MyD88: 0.365 and 0.508; IRF7: 0.078 and 0.01, respectively, p<0.05). These findings highlight the role of TLR7 and LL-37 in co-infection, emphasizing their impact on dynamics. TLR7 and LL-37 regulate BoAHV- 1 replication, promoting N. caninum replication when both pathogens are present. Fil: Plá, Natalia. Universidad Nacional de Mar del Plata. Facultad de Ciencias Agrarias; Argentina Fil: Burucúa, Mercedes María. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales. Departamento de Biología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Innovación para la Producción Agropecuaria y el Desarrollo Sostenible - Instituto Nacional de Tecnología Agropecuaria. Centro Regional Buenos Aires Sur. Estación Experimental Agropecuaria Balcarce. Instituto de Innovación para la Producción Agropecuaria y el Desarrollo Sostenible; Argentina Fil: Moore, Dadin Prando. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Innovación para la Producción Agropecuaria y el Desarrollo Sostenible - Instituto Nacional de Tecnología Agropecuaria. Centro Regional Buenos Aires Sur. Estación Experimental Agropecuaria Balcarce. Instituto de Innovación para la Producción Agropecuaria y el Desarrollo Sostenible; Argentina. Universidad Nacional de Mar del Plata. Facultad de Cs.agrarias. Departamento de Producción Animal; Argentina Fil: Odeón, Anselmo Carlos. Universidad Nacional de Mar del Plata. Facultad de Cs.agrarias. Departamento de Producción Animal; Argentina Fil: Cobo, Eduardo Ruben. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Quintana, Silvina. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales. Instituto de Investigaciones en Sanidad Producción y Ambiente. - Comisión de Investigaciones Científicas de la Provincia de Buenos Aires. Instituto de Investigaciones en Sanidad Producción y Ambiente; Argentina Fil: Marin, Maia Solange. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Innovación para la Producción Agropecuaria y el Desarrollo Sostenible - Instituto Nacional de Tecnología Agropecuaria. Centro Regional Buenos Aires Sur. Estación Experimental Agropecuaria Balcarce. Instituto de Innovación para la Producción Agropecuaria y el Desarrollo Sostenible; Argentina. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales. Departamento de Biología; Argentina VII International Meeting of Apicomplexan parasites in farm animals - ApiCOWplexa La Plata Argentina Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias |
| description |
Neospora caninum causes significant global economic losses due to abortions in cattle. Immunosuppression induced by viral infection worsens neosporosis. However, the innate immune mechanisms in N. caninum and BoAHV-1 co-infections remain unknown. We previouslyshowed an overexpression of toll-like receptor (TLR) 7 and LL-37 human cathelicidin in this co-infection. This study investigated in vitro effects of bovine alpha-herpesvirus-1 (BoAHV-1) infection on TLRs and cathelicidin antimicrobial peptides signaling, and N. caninum replication in co-infected neuronal cells. Human neuroblastoma SH-SY5Y cells were pretreated with chloroquine (inhibitor of endosomal TLRs, 10 µg/ml), imiquimod (agonist of TLR7, 5 µg/ml),sodium butyrate (promoter of endogenous cathelicidin, 4 mM), or subjected to silencing of LL-37 (shLL-37), followed by infection with the reference strains N. caninum NC-1 (MOI: 0.2) and BoAHV-1 Cooper (MOI: 0.1). At 24 h post-infection, gene expression levels of key immune molecules involved in TLR mediated signaling pathways (NF-κβ, MyD88, IRF3, and IRF7) were assessed by RT-qPCR and tachyzoite replication was examined microscopically. The results indicated that N. caninum replication in coinfection with BoAHV-1 was significantly decreased both intracellularly and extracellularly after chloroquine pretreatment and shLL-37 compared to imiquimod and sodium butyrate treatments. This reduction was accompanied in co-infection by a significant increase, with respect to the control, in MyD88 and IRF3 expression (3.9-fold and 5.5- fold, respectively, p<0.05) after chloroquine treatment, and a decrease in IRF3 expression after shLL-37 (0.409-fold, p<0.05). While treatments with imiquimod and sodium butyrate resulted in a significant decrease, with respect to the control, in the expression of three of the four molecules analyzed (NF-κβ: 0.126 and 0.074; MyD88: 0.365 and 0.508; IRF7: 0.078 and 0.01, respectively, p<0.05). These findings highlight the role of TLR7 and LL-37 in co-infection, emphasizing their impact on dynamics. TLR7 and LL-37 regulate BoAHV- 1 replication, promoting N. caninum replication when both pathogens are present. |
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2024 |
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http://hdl.handle.net/11336/275682 Innate immune response in Neospora caninum and BoAHV-1 coinfection: The interaction of TLRs and cathelicidins; VII International Meeting of Apicomplexan parasites in farm animals - ApiCOWplexa; La Plata; Argentina; 2024; 121-122 978-950-34-2444-5 CONICET Digital CONICET |
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Innate immune response in Neospora caninum and BoAHV-1 coinfection: The interaction of TLRs and cathelicidins; VII International Meeting of Apicomplexan parasites in farm animals - ApiCOWplexa; La Plata; Argentina; 2024; 121-122 978-950-34-2444-5 CONICET Digital CONICET |
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