Phylogenetic analysis of bluetongue virus serotype 4 field isolates from Argentina.
- Autores
- Legisa, Danilo Mario; González, Fernanda Gabriela; De Stefano, G.; Pereda, Ariel Julián; Dus Santos, María José
- Año de publicación
- 2013
- Idioma
- inglés
- Tipo de recurso
- artículo
- Estado
- versión publicada
- Descripción
- Bluetongue (BT) is an insect-transmitted viral disease of ruminant species, which represents a major barrier to the international trade of animals and their products. Bluetongue virus (BTV) has a genome composed of ten linear segments of double-stranded RNA (dsRNA), which code for at least ten different viral proteins. In South America, serological evidence for the presence of BTV has been found in Peru, Argentina, Brazil, Ecuador and Chile. Brazil and Argentina are the only South American countries where BTV has been isolated. In Brazil, only one BTV isolate, serotype 12, has been reported, whereas in Argentina five BTV serotype 4 isolates have been obtained from cattle without clinical signs. Three of these five isolates were isolated during 1999-2001, whereas two of them were obtained as part of the present work. This study describes sequence comparisons and phylogenetic analyses of segments 2, 3, 6, 7 and 10 of the first Argentinean field isolates of BTV. The analysis of segments 2 and 6 resulted in a single cluster of Argentinean sequences into the serotype 4 clade. In addition, the Argentinean sequences grouped within the nucleotype A clade, along with reference strains. The analysis of segments 3, 7 and 10 showed that the Argentinean isolates grouped into the western topotype, indicating that the circulating virus had an African/European origin. Phylogenetic analysis revealed that the Argentinean sequences present a South American genetic identity, suggesting an independent lineage evolution.
Fil: Legisa, Danilo Mario. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Virología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: González, Fernanda Gabriela. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Virología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: De Stefano, G.. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Virología; Argentina
Fil: Pereda, Ariel Julián. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Virología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Dus Santos, María José. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Virología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina - Materia
-
Argentina
Phylogenetic
Veterinary
Bluetongue - Nivel de accesibilidad
- acceso abierto
- Condiciones de uso
- https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
- Repositorio
- Institución
- Consejo Nacional de Investigaciones Científicas y Técnicas
- OAI Identificador
- oai:ri.conicet.gov.ar:11336/23967
Ver los metadatos del registro completo
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Phylogenetic analysis of bluetongue virus serotype 4 field isolates from Argentina.Legisa, Danilo MarioGonzález, Fernanda GabrielaDe Stefano, G.Pereda, Ariel JuliánDus Santos, María JoséArgentinaPhylogeneticVeterinaryBluetongueBluetongue (BT) is an insect-transmitted viral disease of ruminant species, which represents a major barrier to the international trade of animals and their products. Bluetongue virus (BTV) has a genome composed of ten linear segments of double-stranded RNA (dsRNA), which code for at least ten different viral proteins. In South America, serological evidence for the presence of BTV has been found in Peru, Argentina, Brazil, Ecuador and Chile. Brazil and Argentina are the only South American countries where BTV has been isolated. In Brazil, only one BTV isolate, serotype 12, has been reported, whereas in Argentina five BTV serotype 4 isolates have been obtained from cattle without clinical signs. Three of these five isolates were isolated during 1999-2001, whereas two of them were obtained as part of the present work. This study describes sequence comparisons and phylogenetic analyses of segments 2, 3, 6, 7 and 10 of the first Argentinean field isolates of BTV. The analysis of segments 2 and 6 resulted in a single cluster of Argentinean sequences into the serotype 4 clade. In addition, the Argentinean sequences grouped within the nucleotype A clade, along with reference strains. The analysis of segments 3, 7 and 10 showed that the Argentinean isolates grouped into the western topotype, indicating that the circulating virus had an African/European origin. Phylogenetic analysis revealed that the Argentinean sequences present a South American genetic identity, suggesting an independent lineage evolution.Fil: Legisa, Danilo Mario. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Virología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: González, Fernanda Gabriela. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Virología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: De Stefano, G.. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Virología; ArgentinaFil: Pereda, Ariel Julián. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Virología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Dus Santos, María José. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Virología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaSociety for General Microbiology2013-03info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/23967Legisa, Danilo Mario; González, Fernanda Gabriela; De Stefano, G.; Pereda, Ariel Julián; Dus Santos, María José; Phylogenetic analysis of bluetongue virus serotype 4 field isolates from Argentina.; Society for General Microbiology; Journal of General Virology; 94; 3; 3-2013; 652-6620022-1317CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/http://jgv.microbiologyresearch.org/content/journal/jgv/10.1099/vir.0.046896-0info:eu-repo/semantics/altIdentifier/doi/10.1099/vir.0.046896-0info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:11:41Zoai:ri.conicet.gov.ar:11336/23967instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:11:41.399CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse |
dc.title.none.fl_str_mv |
Phylogenetic analysis of bluetongue virus serotype 4 field isolates from Argentina. |
title |
Phylogenetic analysis of bluetongue virus serotype 4 field isolates from Argentina. |
spellingShingle |
Phylogenetic analysis of bluetongue virus serotype 4 field isolates from Argentina. Legisa, Danilo Mario Argentina Phylogenetic Veterinary Bluetongue |
title_short |
Phylogenetic analysis of bluetongue virus serotype 4 field isolates from Argentina. |
title_full |
Phylogenetic analysis of bluetongue virus serotype 4 field isolates from Argentina. |
title_fullStr |
Phylogenetic analysis of bluetongue virus serotype 4 field isolates from Argentina. |
title_full_unstemmed |
Phylogenetic analysis of bluetongue virus serotype 4 field isolates from Argentina. |
title_sort |
Phylogenetic analysis of bluetongue virus serotype 4 field isolates from Argentina. |
dc.creator.none.fl_str_mv |
Legisa, Danilo Mario González, Fernanda Gabriela De Stefano, G. Pereda, Ariel Julián Dus Santos, María José |
author |
Legisa, Danilo Mario |
author_facet |
Legisa, Danilo Mario González, Fernanda Gabriela De Stefano, G. Pereda, Ariel Julián Dus Santos, María José |
author_role |
author |
author2 |
González, Fernanda Gabriela De Stefano, G. Pereda, Ariel Julián Dus Santos, María José |
author2_role |
author author author author |
dc.subject.none.fl_str_mv |
Argentina Phylogenetic Veterinary Bluetongue |
topic |
Argentina Phylogenetic Veterinary Bluetongue |
dc.description.none.fl_txt_mv |
Bluetongue (BT) is an insect-transmitted viral disease of ruminant species, which represents a major barrier to the international trade of animals and their products. Bluetongue virus (BTV) has a genome composed of ten linear segments of double-stranded RNA (dsRNA), which code for at least ten different viral proteins. In South America, serological evidence for the presence of BTV has been found in Peru, Argentina, Brazil, Ecuador and Chile. Brazil and Argentina are the only South American countries where BTV has been isolated. In Brazil, only one BTV isolate, serotype 12, has been reported, whereas in Argentina five BTV serotype 4 isolates have been obtained from cattle without clinical signs. Three of these five isolates were isolated during 1999-2001, whereas two of them were obtained as part of the present work. This study describes sequence comparisons and phylogenetic analyses of segments 2, 3, 6, 7 and 10 of the first Argentinean field isolates of BTV. The analysis of segments 2 and 6 resulted in a single cluster of Argentinean sequences into the serotype 4 clade. In addition, the Argentinean sequences grouped within the nucleotype A clade, along with reference strains. The analysis of segments 3, 7 and 10 showed that the Argentinean isolates grouped into the western topotype, indicating that the circulating virus had an African/European origin. Phylogenetic analysis revealed that the Argentinean sequences present a South American genetic identity, suggesting an independent lineage evolution. Fil: Legisa, Danilo Mario. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Virología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: González, Fernanda Gabriela. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Virología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: De Stefano, G.. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Virología; Argentina Fil: Pereda, Ariel Julián. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Virología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Dus Santos, María José. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Virología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina |
description |
Bluetongue (BT) is an insect-transmitted viral disease of ruminant species, which represents a major barrier to the international trade of animals and their products. Bluetongue virus (BTV) has a genome composed of ten linear segments of double-stranded RNA (dsRNA), which code for at least ten different viral proteins. In South America, serological evidence for the presence of BTV has been found in Peru, Argentina, Brazil, Ecuador and Chile. Brazil and Argentina are the only South American countries where BTV has been isolated. In Brazil, only one BTV isolate, serotype 12, has been reported, whereas in Argentina five BTV serotype 4 isolates have been obtained from cattle without clinical signs. Three of these five isolates were isolated during 1999-2001, whereas two of them were obtained as part of the present work. This study describes sequence comparisons and phylogenetic analyses of segments 2, 3, 6, 7 and 10 of the first Argentinean field isolates of BTV. The analysis of segments 2 and 6 resulted in a single cluster of Argentinean sequences into the serotype 4 clade. In addition, the Argentinean sequences grouped within the nucleotype A clade, along with reference strains. The analysis of segments 3, 7 and 10 showed that the Argentinean isolates grouped into the western topotype, indicating that the circulating virus had an African/European origin. Phylogenetic analysis revealed that the Argentinean sequences present a South American genetic identity, suggesting an independent lineage evolution. |
publishDate |
2013 |
dc.date.none.fl_str_mv |
2013-03 |
dc.type.none.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://purl.org/coar/resource_type/c_6501 info:ar-repo/semantics/articulo |
format |
article |
status_str |
publishedVersion |
dc.identifier.none.fl_str_mv |
http://hdl.handle.net/11336/23967 Legisa, Danilo Mario; González, Fernanda Gabriela; De Stefano, G.; Pereda, Ariel Julián; Dus Santos, María José; Phylogenetic analysis of bluetongue virus serotype 4 field isolates from Argentina.; Society for General Microbiology; Journal of General Virology; 94; 3; 3-2013; 652-662 0022-1317 CONICET Digital CONICET |
url |
http://hdl.handle.net/11336/23967 |
identifier_str_mv |
Legisa, Danilo Mario; González, Fernanda Gabriela; De Stefano, G.; Pereda, Ariel Julián; Dus Santos, María José; Phylogenetic analysis of bluetongue virus serotype 4 field isolates from Argentina.; Society for General Microbiology; Journal of General Virology; 94; 3; 3-2013; 652-662 0022-1317 CONICET Digital CONICET |
dc.language.none.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
info:eu-repo/semantics/altIdentifier/url/http://jgv.microbiologyresearch.org/content/journal/jgv/10.1099/vir.0.046896-0 info:eu-repo/semantics/altIdentifier/doi/10.1099/vir.0.046896-0 |
dc.rights.none.fl_str_mv |
info:eu-repo/semantics/openAccess https://creativecommons.org/licenses/by-nc-sa/2.5/ar/ |
eu_rights_str_mv |
openAccess |
rights_invalid_str_mv |
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/ |
dc.format.none.fl_str_mv |
application/pdf application/pdf application/pdf application/pdf |
dc.publisher.none.fl_str_mv |
Society for General Microbiology |
publisher.none.fl_str_mv |
Society for General Microbiology |
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reponame:CONICET Digital (CONICET) instname:Consejo Nacional de Investigaciones Científicas y Técnicas |
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Consejo Nacional de Investigaciones Científicas y Técnicas |
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CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas |
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dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar |
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