Phylogenetic analysis of bluetongue virus serotype 4 field isolates from Argentina.

Autores
Legisa, Danilo Mario; González, Fernanda Gabriela; De Stefano, G.; Pereda, Ariel Julián; Dus Santos, María José
Año de publicación
2013
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Bluetongue (BT) is an insect-transmitted viral disease of ruminant species, which represents a major barrier to the international trade of animals and their products. Bluetongue virus (BTV) has a genome composed of ten linear segments of double-stranded RNA (dsRNA), which code for at least ten different viral proteins. In South America, serological evidence for the presence of BTV has been found in Peru, Argentina, Brazil, Ecuador and Chile. Brazil and Argentina are the only South American countries where BTV has been isolated. In Brazil, only one BTV isolate, serotype 12, has been reported, whereas in Argentina five BTV serotype 4 isolates have been obtained from cattle without clinical signs. Three of these five isolates were isolated during 1999-2001, whereas two of them were obtained as part of the present work. This study describes sequence comparisons and phylogenetic analyses of segments 2, 3, 6, 7 and 10 of the first Argentinean field isolates of BTV. The analysis of segments 2 and 6 resulted in a single cluster of Argentinean sequences into the serotype 4 clade. In addition, the Argentinean sequences grouped within the nucleotype A clade, along with reference strains. The analysis of segments 3, 7 and 10 showed that the Argentinean isolates grouped into the western topotype, indicating that the circulating virus had an African/European origin. Phylogenetic analysis revealed that the Argentinean sequences present a South American genetic identity, suggesting an independent lineage evolution.
Fil: Legisa, Danilo Mario. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Virología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: González, Fernanda Gabriela. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Virología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: De Stefano, G.. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Virología; Argentina
Fil: Pereda, Ariel Julián. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Virología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Dus Santos, María José. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Virología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Materia
Argentina
Phylogenetic
Veterinary
Bluetongue
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/23967
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/23967
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Phylogenetic analysis of bluetongue virus serotype 4 field isolates from Argentina.Legisa, Danilo MarioGonzález, Fernanda GabrielaDe Stefano, G.Pereda, Ariel JuliánDus Santos, María JoséArgentinaPhylogeneticVeterinaryBluetongueBluetongue (BT) is an insect-transmitted viral disease of ruminant species, which represents a major barrier to the international trade of animals and their products. Bluetongue virus (BTV) has a genome composed of ten linear segments of double-stranded RNA (dsRNA), which code for at least ten different viral proteins. In South America, serological evidence for the presence of BTV has been found in Peru, Argentina, Brazil, Ecuador and Chile. Brazil and Argentina are the only South American countries where BTV has been isolated. In Brazil, only one BTV isolate, serotype 12, has been reported, whereas in Argentina five BTV serotype 4 isolates have been obtained from cattle without clinical signs. Three of these five isolates were isolated during 1999-2001, whereas two of them were obtained as part of the present work. This study describes sequence comparisons and phylogenetic analyses of segments 2, 3, 6, 7 and 10 of the first Argentinean field isolates of BTV. The analysis of segments 2 and 6 resulted in a single cluster of Argentinean sequences into the serotype 4 clade. In addition, the Argentinean sequences grouped within the nucleotype A clade, along with reference strains. The analysis of segments 3, 7 and 10 showed that the Argentinean isolates grouped into the western topotype, indicating that the circulating virus had an African/European origin. Phylogenetic analysis revealed that the Argentinean sequences present a South American genetic identity, suggesting an independent lineage evolution.Fil: Legisa, Danilo Mario. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Virología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: González, Fernanda Gabriela. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Virología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: De Stefano, G.. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Virología; ArgentinaFil: Pereda, Ariel Julián. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Virología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Dus Santos, María José. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Virología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaSociety for General Microbiology2013-03info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/23967Legisa, Danilo Mario; González, Fernanda Gabriela; De Stefano, G.; Pereda, Ariel Julián; Dus Santos, María José; Phylogenetic analysis of bluetongue virus serotype 4 field isolates from Argentina.; Society for General Microbiology; Journal of General Virology; 94; 3; 3-2013; 652-6620022-1317CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/http://jgv.microbiologyresearch.org/content/journal/jgv/10.1099/vir.0.046896-0info:eu-repo/semantics/altIdentifier/doi/10.1099/vir.0.046896-0info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-22T11:41:37Zoai:ri.conicet.gov.ar:11336/23967instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-22 11:41:38.111CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Phylogenetic analysis of bluetongue virus serotype 4 field isolates from Argentina.
title Phylogenetic analysis of bluetongue virus serotype 4 field isolates from Argentina.
spellingShingle Phylogenetic analysis of bluetongue virus serotype 4 field isolates from Argentina.
Legisa, Danilo Mario
Argentina
Phylogenetic
Veterinary
Bluetongue
title_short Phylogenetic analysis of bluetongue virus serotype 4 field isolates from Argentina.
title_full Phylogenetic analysis of bluetongue virus serotype 4 field isolates from Argentina.
title_fullStr Phylogenetic analysis of bluetongue virus serotype 4 field isolates from Argentina.
title_full_unstemmed Phylogenetic analysis of bluetongue virus serotype 4 field isolates from Argentina.
title_sort Phylogenetic analysis of bluetongue virus serotype 4 field isolates from Argentina.
dc.creator.none.fl_str_mv Legisa, Danilo Mario
González, Fernanda Gabriela
De Stefano, G.
Pereda, Ariel Julián
Dus Santos, María José
author Legisa, Danilo Mario
author_facet Legisa, Danilo Mario
González, Fernanda Gabriela
De Stefano, G.
Pereda, Ariel Julián
Dus Santos, María José
author_role author
author2 González, Fernanda Gabriela
De Stefano, G.
Pereda, Ariel Julián
Dus Santos, María José
author2_role author
author
author
author
dc.subject.none.fl_str_mv Argentina
Phylogenetic
Veterinary
Bluetongue
topic Argentina
Phylogenetic
Veterinary
Bluetongue
dc.description.none.fl_txt_mv Bluetongue (BT) is an insect-transmitted viral disease of ruminant species, which represents a major barrier to the international trade of animals and their products. Bluetongue virus (BTV) has a genome composed of ten linear segments of double-stranded RNA (dsRNA), which code for at least ten different viral proteins. In South America, serological evidence for the presence of BTV has been found in Peru, Argentina, Brazil, Ecuador and Chile. Brazil and Argentina are the only South American countries where BTV has been isolated. In Brazil, only one BTV isolate, serotype 12, has been reported, whereas in Argentina five BTV serotype 4 isolates have been obtained from cattle without clinical signs. Three of these five isolates were isolated during 1999-2001, whereas two of them were obtained as part of the present work. This study describes sequence comparisons and phylogenetic analyses of segments 2, 3, 6, 7 and 10 of the first Argentinean field isolates of BTV. The analysis of segments 2 and 6 resulted in a single cluster of Argentinean sequences into the serotype 4 clade. In addition, the Argentinean sequences grouped within the nucleotype A clade, along with reference strains. The analysis of segments 3, 7 and 10 showed that the Argentinean isolates grouped into the western topotype, indicating that the circulating virus had an African/European origin. Phylogenetic analysis revealed that the Argentinean sequences present a South American genetic identity, suggesting an independent lineage evolution.
Fil: Legisa, Danilo Mario. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Virología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: González, Fernanda Gabriela. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Virología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: De Stefano, G.. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Virología; Argentina
Fil: Pereda, Ariel Julián. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Virología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Dus Santos, María José. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Virología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
description Bluetongue (BT) is an insect-transmitted viral disease of ruminant species, which represents a major barrier to the international trade of animals and their products. Bluetongue virus (BTV) has a genome composed of ten linear segments of double-stranded RNA (dsRNA), which code for at least ten different viral proteins. In South America, serological evidence for the presence of BTV has been found in Peru, Argentina, Brazil, Ecuador and Chile. Brazil and Argentina are the only South American countries where BTV has been isolated. In Brazil, only one BTV isolate, serotype 12, has been reported, whereas in Argentina five BTV serotype 4 isolates have been obtained from cattle without clinical signs. Three of these five isolates were isolated during 1999-2001, whereas two of them were obtained as part of the present work. This study describes sequence comparisons and phylogenetic analyses of segments 2, 3, 6, 7 and 10 of the first Argentinean field isolates of BTV. The analysis of segments 2 and 6 resulted in a single cluster of Argentinean sequences into the serotype 4 clade. In addition, the Argentinean sequences grouped within the nucleotype A clade, along with reference strains. The analysis of segments 3, 7 and 10 showed that the Argentinean isolates grouped into the western topotype, indicating that the circulating virus had an African/European origin. Phylogenetic analysis revealed that the Argentinean sequences present a South American genetic identity, suggesting an independent lineage evolution.
publishDate 2013
dc.date.none.fl_str_mv 2013-03
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/23967
Legisa, Danilo Mario; González, Fernanda Gabriela; De Stefano, G.; Pereda, Ariel Julián; Dus Santos, María José; Phylogenetic analysis of bluetongue virus serotype 4 field isolates from Argentina.; Society for General Microbiology; Journal of General Virology; 94; 3; 3-2013; 652-662
0022-1317
CONICET Digital
CONICET
url http://hdl.handle.net/11336/23967
identifier_str_mv Legisa, Danilo Mario; González, Fernanda Gabriela; De Stefano, G.; Pereda, Ariel Julián; Dus Santos, María José; Phylogenetic analysis of bluetongue virus serotype 4 field isolates from Argentina.; Society for General Microbiology; Journal of General Virology; 94; 3; 3-2013; 652-662
0022-1317
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/http://jgv.microbiologyresearch.org/content/journal/jgv/10.1099/vir.0.046896-0
info:eu-repo/semantics/altIdentifier/doi/10.1099/vir.0.046896-0
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Society for General Microbiology
publisher.none.fl_str_mv Society for General Microbiology
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 12.982451

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