Karyotype analysis and DNA content in some species of Chrysolaena (Vernonieae, Asteraceae)

• Autores: Via Do Pico, Gisela Mariel; Dematteis, Massimiliano
• Año de publicación: 2013
• Idioma: inglés
• Tipo de recurso: artículo
• Materia: Chromosomes; Compositae; Flow Cytometry; Vernonia
• Institución: Consejo Nacional de Investigaciones Científicas y Técnicas

Cytogenetic characterization by karyotyping and determination of DNA content by flow cytometry of five species of Chrysolaena (Vernonieae, Asteraceae) was performed. This is the first study of nuclear DNA content realized in the genus. The 2C-values were compared with the ploidy level and the total karyotype length (TKL) of each species. Mitotic analysis revealed a base chromosome number x = 10 for all entities and different ploidy levels, from diploid (2n = 2x = 20) to octoploid (2n = 8x = 80). All species showed bimodal karyotypes composed of metacentric and submetacentric chromosomes. The average chromosome size (ML) varied from 1.86 μm to 2.70 μm, while the TKL ranged from 18.65 μm to 80.55 μm. The intrachromosomal asymmetry index (A1) varied from 0.27 to 0.38, while the interchromosomal asymmetry index (A2) ranged from 0.19 to 0.25. A new cytotype is reported for the first time for C. propinqua. Accessory chromosomes found inC. verbascifolia, C. cognata, C. flexuosa, and C. propinqua are also reported as new.
Fil: Via Do Pico, Gisela Mariel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Nordeste. Instituto de Botánica del Nordeste (i); Argentina;
Fil: Dematteis, Massimiliano. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Nordeste. Instituto de Botánica del Nordeste (i); Argentina;