A 43-nucleotide U-rich element in 3′-untranslated region of large number of Trypanosoma cruzi transcripts is important for mRNA Abundance in intracellular amastigotes

Autores
Li, Zhu Hong; de Gaudenzi, Javier Gerardo; Alvarez, Vanina Eder; Mendiondo, Nicolas; Wang, Haiming; Kissinger, Jessica C.; Frasch, Alberto Carlos C.; Docampo, Roberto
Año de publicación
2012
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Trypanosoma cruzi, the agent of Chagas disease, does not seem to control gene expression through regulation of transcription initiation and makes use of post-transcriptional mechanisms. We report here a 43-nt U-rich RNA element located in the 3′-untranslated region (3′-UTR) of a large number of T. cruzi mRNAs that is important for mRNA abundance in the intracellular amastigote stage of the parasite. Whole genome scan analysis, differential display RT-PCR, Northern blot, and RT-PCR analyses were used to determine the transcript levels of more than 900 U-rich-containing mRNAs of large gene families as well as single and low copy number genes. Our results indicate that the 43-nt U-richmRNAelement is preferentially present in amastigotes. The cis-element of a protein kinase 3′-UTR but not its mutated version promoted the expression of the green fluorescent protein reporter gene in amastigotes. The regulatory ciselement, but not its mutated version, was also shown to interact with the trypanosome-specific RNA-binding protein (RBP) TcUBP1 but not with other related RBPs. Co-immunoprecipitation experiments of TcUBP1-containing ribonucleoprotein complexes formed in vivo validated the interaction with representative endogenous RNAs having the element. These results suggest that this 43-nt U-rich element together with other yet unidentified sequences might be involved in the modulation of abundance and/or translation of subsets of transcripts in the amastigote stage.
Fil: Li, Zhu Hong. University of Georgia; Estados Unidos
Fil: de Gaudenzi, Javier Gerardo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Biotecnológicas. Instituto de Investigaciones Biotecnológicas "Dr. Raúl Alfonsín" (sede Chascomús). Universidad Nacional de San Martín. Instituto de Investigaciones Biotecnológicas. Instituto de Investigaciones Biotecnológicas "Dr. Raúl Alfonsín" (sede Chascomús); Argentina
Fil: Alvarez, Vanina Eder. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Biotecnológicas. Instituto de Investigaciones Biotecnológicas "Dr. Raúl Alfonsín" (sede Chascomús). Universidad Nacional de San Martín. Instituto de Investigaciones Biotecnológicas. Instituto de Investigaciones Biotecnológicas "Dr. Raúl Alfonsín" (sede Chascomús); Argentina
Fil: Mendiondo, Nicolas. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Biotecnológicas. Instituto de Investigaciones Biotecnológicas "Dr. Raúl Alfonsín" (sede Chascomús). Universidad Nacional de San Martín. Instituto de Investigaciones Biotecnológicas. Instituto de Investigaciones Biotecnológicas "Dr. Raúl Alfonsín" (sede Chascomús); Argentina
Fil: Wang, Haiming. University of Georgia; Estados Unidos
Fil: Kissinger, Jessica C.. University of Georgia; Estados Unidos
Fil: Frasch, Alberto Carlos C.. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Biotecnológicas. Instituto de Investigaciones Biotecnológicas "Dr. Raúl Alfonsín" (sede Chascomús). Universidad Nacional de San Martín. Instituto de Investigaciones Biotecnológicas. Instituto de Investigaciones Biotecnológicas "Dr. Raúl Alfonsín" (sede Chascomús); Argentina
Fil: Docampo, Roberto. University of Georgia; Estados Unidos
Materia
GENE EXPRESSION
PARASITE
RNA ABUNDANCE
RNA-BINDING PROTEIN
TRYPANOSOMA BRUCEI
TRYPANOSOME
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/93465
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/93465
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling A 43-nucleotide U-rich element in 3′-untranslated region of large number of Trypanosoma cruzi transcripts is important for mRNA Abundance in intracellular amastigotesLi, Zhu Hongde Gaudenzi, Javier GerardoAlvarez, Vanina EderMendiondo, NicolasWang, HaimingKissinger, Jessica C.Frasch, Alberto Carlos C.Docampo, RobertoGENE EXPRESSIONPARASITERNA ABUNDANCERNA-BINDING PROTEINTRYPANOSOMA BRUCEITRYPANOSOMEhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Trypanosoma cruzi, the agent of Chagas disease, does not seem to control gene expression through regulation of transcription initiation and makes use of post-transcriptional mechanisms. We report here a 43-nt U-rich RNA element located in the 3′-untranslated region (3′-UTR) of a large number of T. cruzi mRNAs that is important for mRNA abundance in the intracellular amastigote stage of the parasite. Whole genome scan analysis, differential display RT-PCR, Northern blot, and RT-PCR analyses were used to determine the transcript levels of more than 900 U-rich-containing mRNAs of large gene families as well as single and low copy number genes. Our results indicate that the 43-nt U-richmRNAelement is preferentially present in amastigotes. The cis-element of a protein kinase 3′-UTR but not its mutated version promoted the expression of the green fluorescent protein reporter gene in amastigotes. The regulatory ciselement, but not its mutated version, was also shown to interact with the trypanosome-specific RNA-binding protein (RBP) TcUBP1 but not with other related RBPs. Co-immunoprecipitation experiments of TcUBP1-containing ribonucleoprotein complexes formed in vivo validated the interaction with representative endogenous RNAs having the element. These results suggest that this 43-nt U-rich element together with other yet unidentified sequences might be involved in the modulation of abundance and/or translation of subsets of transcripts in the amastigote stage.Fil: Li, Zhu Hong. University of Georgia; Estados UnidosFil: de Gaudenzi, Javier Gerardo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Biotecnológicas. Instituto de Investigaciones Biotecnológicas "Dr. Raúl Alfonsín" (sede Chascomús). Universidad Nacional de San Martín. Instituto de Investigaciones Biotecnológicas. Instituto de Investigaciones Biotecnológicas "Dr. Raúl Alfonsín" (sede Chascomús); ArgentinaFil: Alvarez, Vanina Eder. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Biotecnológicas. Instituto de Investigaciones Biotecnológicas "Dr. Raúl Alfonsín" (sede Chascomús). Universidad Nacional de San Martín. Instituto de Investigaciones Biotecnológicas. Instituto de Investigaciones Biotecnológicas "Dr. Raúl Alfonsín" (sede Chascomús); ArgentinaFil: Mendiondo, Nicolas. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Biotecnológicas. Instituto de Investigaciones Biotecnológicas "Dr. Raúl Alfonsín" (sede Chascomús). Universidad Nacional de San Martín. Instituto de Investigaciones Biotecnológicas. Instituto de Investigaciones Biotecnológicas "Dr. Raúl Alfonsín" (sede Chascomús); ArgentinaFil: Wang, Haiming. University of Georgia; Estados UnidosFil: Kissinger, Jessica C.. University of Georgia; Estados UnidosFil: Frasch, Alberto Carlos C.. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Biotecnológicas. Instituto de Investigaciones Biotecnológicas "Dr. Raúl Alfonsín" (sede Chascomús). Universidad Nacional de San Martín. Instituto de Investigaciones Biotecnológicas. Instituto de Investigaciones Biotecnológicas "Dr. Raúl Alfonsín" (sede Chascomús); ArgentinaFil: Docampo, Roberto. University of Georgia; Estados UnidosAmerican Society for Biochemistry and Molecular Biology2012-06-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/93465Li, Zhu Hong; de Gaudenzi, Javier Gerardo; Alvarez, Vanina Eder; Mendiondo, Nicolas; Wang, Haiming; et al.; A 43-nucleotide U-rich element in 3′-untranslated region of large number of Trypanosoma cruzi transcripts is important for mRNA Abundance in intracellular amastigotes; American Society for Biochemistry and Molecular Biology; Journal of Biological Chemistry (online); 287; 23; 1-6-2012; 19058-190690021-92581083-351XCONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/http://www.jbc.org/content/287/23/19058info:eu-repo/semantics/altIdentifier/url/https://www.ncbi.nlm.nih.gov/pubmed/22500021info:eu-repo/semantics/altIdentifier/doi/10.1074/jbc.M111.338699info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T09:57:20Zoai:ri.conicet.gov.ar:11336/93465instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 09:57:20.908CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv A 43-nucleotide U-rich element in 3′-untranslated region of large number of Trypanosoma cruzi transcripts is important for mRNA Abundance in intracellular amastigotes
title A 43-nucleotide U-rich element in 3′-untranslated region of large number of Trypanosoma cruzi transcripts is important for mRNA Abundance in intracellular amastigotes
spellingShingle A 43-nucleotide U-rich element in 3′-untranslated region of large number of Trypanosoma cruzi transcripts is important for mRNA Abundance in intracellular amastigotes
Li, Zhu Hong
GENE EXPRESSION
PARASITE
RNA ABUNDANCE
RNA-BINDING PROTEIN
TRYPANOSOMA BRUCEI
TRYPANOSOME
title_short A 43-nucleotide U-rich element in 3′-untranslated region of large number of Trypanosoma cruzi transcripts is important for mRNA Abundance in intracellular amastigotes
title_full A 43-nucleotide U-rich element in 3′-untranslated region of large number of Trypanosoma cruzi transcripts is important for mRNA Abundance in intracellular amastigotes
title_fullStr A 43-nucleotide U-rich element in 3′-untranslated region of large number of Trypanosoma cruzi transcripts is important for mRNA Abundance in intracellular amastigotes
title_full_unstemmed A 43-nucleotide U-rich element in 3′-untranslated region of large number of Trypanosoma cruzi transcripts is important for mRNA Abundance in intracellular amastigotes
title_sort A 43-nucleotide U-rich element in 3′-untranslated region of large number of Trypanosoma cruzi transcripts is important for mRNA Abundance in intracellular amastigotes
dc.creator.none.fl_str_mv Li, Zhu Hong
de Gaudenzi, Javier Gerardo
Alvarez, Vanina Eder
Mendiondo, Nicolas
Wang, Haiming
Kissinger, Jessica C.
Frasch, Alberto Carlos C.
Docampo, Roberto
author Li, Zhu Hong
author_facet Li, Zhu Hong
de Gaudenzi, Javier Gerardo
Alvarez, Vanina Eder
Mendiondo, Nicolas
Wang, Haiming
Kissinger, Jessica C.
Frasch, Alberto Carlos C.
Docampo, Roberto
author_role author
author2 de Gaudenzi, Javier Gerardo
Alvarez, Vanina Eder
Mendiondo, Nicolas
Wang, Haiming
Kissinger, Jessica C.
Frasch, Alberto Carlos C.
Docampo, Roberto
author2_role author
author
author
author
author
author
author
dc.subject.none.fl_str_mv GENE EXPRESSION
PARASITE
RNA ABUNDANCE
RNA-BINDING PROTEIN
TRYPANOSOMA BRUCEI
TRYPANOSOME
topic GENE EXPRESSION
PARASITE
RNA ABUNDANCE
RNA-BINDING PROTEIN
TRYPANOSOMA BRUCEI
TRYPANOSOME
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Trypanosoma cruzi, the agent of Chagas disease, does not seem to control gene expression through regulation of transcription initiation and makes use of post-transcriptional mechanisms. We report here a 43-nt U-rich RNA element located in the 3′-untranslated region (3′-UTR) of a large number of T. cruzi mRNAs that is important for mRNA abundance in the intracellular amastigote stage of the parasite. Whole genome scan analysis, differential display RT-PCR, Northern blot, and RT-PCR analyses were used to determine the transcript levels of more than 900 U-rich-containing mRNAs of large gene families as well as single and low copy number genes. Our results indicate that the 43-nt U-richmRNAelement is preferentially present in amastigotes. The cis-element of a protein kinase 3′-UTR but not its mutated version promoted the expression of the green fluorescent protein reporter gene in amastigotes. The regulatory ciselement, but not its mutated version, was also shown to interact with the trypanosome-specific RNA-binding protein (RBP) TcUBP1 but not with other related RBPs. Co-immunoprecipitation experiments of TcUBP1-containing ribonucleoprotein complexes formed in vivo validated the interaction with representative endogenous RNAs having the element. These results suggest that this 43-nt U-rich element together with other yet unidentified sequences might be involved in the modulation of abundance and/or translation of subsets of transcripts in the amastigote stage.
Fil: Li, Zhu Hong. University of Georgia; Estados Unidos
Fil: de Gaudenzi, Javier Gerardo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Biotecnológicas. Instituto de Investigaciones Biotecnológicas "Dr. Raúl Alfonsín" (sede Chascomús). Universidad Nacional de San Martín. Instituto de Investigaciones Biotecnológicas. Instituto de Investigaciones Biotecnológicas "Dr. Raúl Alfonsín" (sede Chascomús); Argentina
Fil: Alvarez, Vanina Eder. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Biotecnológicas. Instituto de Investigaciones Biotecnológicas "Dr. Raúl Alfonsín" (sede Chascomús). Universidad Nacional de San Martín. Instituto de Investigaciones Biotecnológicas. Instituto de Investigaciones Biotecnológicas "Dr. Raúl Alfonsín" (sede Chascomús); Argentina
Fil: Mendiondo, Nicolas. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Biotecnológicas. Instituto de Investigaciones Biotecnológicas "Dr. Raúl Alfonsín" (sede Chascomús). Universidad Nacional de San Martín. Instituto de Investigaciones Biotecnológicas. Instituto de Investigaciones Biotecnológicas "Dr. Raúl Alfonsín" (sede Chascomús); Argentina
Fil: Wang, Haiming. University of Georgia; Estados Unidos
Fil: Kissinger, Jessica C.. University of Georgia; Estados Unidos
Fil: Frasch, Alberto Carlos C.. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Biotecnológicas. Instituto de Investigaciones Biotecnológicas "Dr. Raúl Alfonsín" (sede Chascomús). Universidad Nacional de San Martín. Instituto de Investigaciones Biotecnológicas. Instituto de Investigaciones Biotecnológicas "Dr. Raúl Alfonsín" (sede Chascomús); Argentina
Fil: Docampo, Roberto. University of Georgia; Estados Unidos
description Trypanosoma cruzi, the agent of Chagas disease, does not seem to control gene expression through regulation of transcription initiation and makes use of post-transcriptional mechanisms. We report here a 43-nt U-rich RNA element located in the 3′-untranslated region (3′-UTR) of a large number of T. cruzi mRNAs that is important for mRNA abundance in the intracellular amastigote stage of the parasite. Whole genome scan analysis, differential display RT-PCR, Northern blot, and RT-PCR analyses were used to determine the transcript levels of more than 900 U-rich-containing mRNAs of large gene families as well as single and low copy number genes. Our results indicate that the 43-nt U-richmRNAelement is preferentially present in amastigotes. The cis-element of a protein kinase 3′-UTR but not its mutated version promoted the expression of the green fluorescent protein reporter gene in amastigotes. The regulatory ciselement, but not its mutated version, was also shown to interact with the trypanosome-specific RNA-binding protein (RBP) TcUBP1 but not with other related RBPs. Co-immunoprecipitation experiments of TcUBP1-containing ribonucleoprotein complexes formed in vivo validated the interaction with representative endogenous RNAs having the element. These results suggest that this 43-nt U-rich element together with other yet unidentified sequences might be involved in the modulation of abundance and/or translation of subsets of transcripts in the amastigote stage.
publishDate 2012
dc.date.none.fl_str_mv 2012-06-01
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/93465
Li, Zhu Hong; de Gaudenzi, Javier Gerardo; Alvarez, Vanina Eder; Mendiondo, Nicolas; Wang, Haiming; et al.; A 43-nucleotide U-rich element in 3′-untranslated region of large number of Trypanosoma cruzi transcripts is important for mRNA Abundance in intracellular amastigotes; American Society for Biochemistry and Molecular Biology; Journal of Biological Chemistry (online); 287; 23; 1-6-2012; 19058-19069
0021-9258
1083-351X
CONICET Digital
CONICET
url http://hdl.handle.net/11336/93465
identifier_str_mv Li, Zhu Hong; de Gaudenzi, Javier Gerardo; Alvarez, Vanina Eder; Mendiondo, Nicolas; Wang, Haiming; et al.; A 43-nucleotide U-rich element in 3′-untranslated region of large number of Trypanosoma cruzi transcripts is important for mRNA Abundance in intracellular amastigotes; American Society for Biochemistry and Molecular Biology; Journal of Biological Chemistry (online); 287; 23; 1-6-2012; 19058-19069
0021-9258
1083-351X
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/http://www.jbc.org/content/287/23/19058
info:eu-repo/semantics/altIdentifier/url/https://www.ncbi.nlm.nih.gov/pubmed/22500021
info:eu-repo/semantics/altIdentifier/doi/10.1074/jbc.M111.338699
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv American Society for Biochemistry and Molecular Biology
publisher.none.fl_str_mv American Society for Biochemistry and Molecular Biology
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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