Insights into the functions and RNA binding of Trypanosoma brucei ZC3H22, RBP9 and DRBD7

Autores
Erben, Esteban Daniel; Leiss, Kevin; Liu, Bin; Gil, Diana Inchaustegui; Helbig, Claudia; Clayton, Christine
Año de publicación
2021
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Trypanosoma brucei is unusually reliant on mRNA-binding proteins to control mRNA fate, because its protein-coding genes lack individual promoters. We here focus on three trypanosome RNA-binding proteins. ZC3H22 is specific to Tsetse fly forms, RBP9 is preferentially expressed in bloodstream forms; and DRBD7 is constitutively expressed. Depletion of RBP9 or DRBD7 did not affect bloodstream-form trypanosome growth. ZC3H22 depletion from procyclic forms caused cell clumping, decreased expression of genes required for cell growth and proliferation, and increased expression of some epimastigote markers. Apart from decreases in mRNAs encoding enzymes of glucose metabolism, levels of most ZC3H22-bound transcripts were unaffected by ZC3H22 depletion. We compared ZC3H22, RBP9 and DRBD7 RNA binding with that of 16 other RNA-binding proteins. ZC3H22, PUF3 and ERBP1 show a preference for ribosomal protein mRNAs. RBP9 preferentially binds mRNAs that are more abundant in bloodstream forms than in procyclic forms. RBP9, ZC3H5, ZC3H30 and DRBD7 prefer mRNAs with long coding regions; UBP1-associated mRNAs have long 3′-untranslated regions; and RRM1 prefers mRNAs with long 3′or 5′-untranslated regions. We suggest that proteins that prefer long mRNAs may have relatively short or degenerate binding sites, and that preferences for A or U increase binding in untranslated regions.
Fil: Erben, Esteban Daniel. Universidad Nacional de San Martín. Instituto de Investigaciones Biotecnológicas. - Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Biotecnológicas; Argentina. Ruprecht Karls Universitat Heidelberg; Alemania
Fil: Leiss, Kevin. Ruprecht Karls Universitat Heidelberg; Alemania
Fil: Liu, Bin. Ruprecht Karls Universitat Heidelberg; Alemania
Fil: Gil, Diana Inchaustegui. Ruprecht Karls Universitat Heidelberg; Alemania
Fil: Helbig, Claudia. Ruprecht Karls Universitat Heidelberg; Alemania
Fil: Clayton, Christine. Ruprecht Karls Universitat Heidelberg; Alemania
Materia
MRNA
RNA-BINDING PROTEIN
TRYPANOSOMA BRUCEI
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/185001
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/185001
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Insights into the functions and RNA binding of Trypanosoma brucei ZC3H22, RBP9 and DRBD7Erben, Esteban DanielLeiss, KevinLiu, BinGil, Diana InchausteguiHelbig, ClaudiaClayton, ChristineMRNARNA-BINDING PROTEINTRYPANOSOMA BRUCEIhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Trypanosoma brucei is unusually reliant on mRNA-binding proteins to control mRNA fate, because its protein-coding genes lack individual promoters. We here focus on three trypanosome RNA-binding proteins. ZC3H22 is specific to Tsetse fly forms, RBP9 is preferentially expressed in bloodstream forms; and DRBD7 is constitutively expressed. Depletion of RBP9 or DRBD7 did not affect bloodstream-form trypanosome growth. ZC3H22 depletion from procyclic forms caused cell clumping, decreased expression of genes required for cell growth and proliferation, and increased expression of some epimastigote markers. Apart from decreases in mRNAs encoding enzymes of glucose metabolism, levels of most ZC3H22-bound transcripts were unaffected by ZC3H22 depletion. We compared ZC3H22, RBP9 and DRBD7 RNA binding with that of 16 other RNA-binding proteins. ZC3H22, PUF3 and ERBP1 show a preference for ribosomal protein mRNAs. RBP9 preferentially binds mRNAs that are more abundant in bloodstream forms than in procyclic forms. RBP9, ZC3H5, ZC3H30 and DRBD7 prefer mRNAs with long coding regions; UBP1-associated mRNAs have long 3′-untranslated regions; and RRM1 prefers mRNAs with long 3′or 5′-untranslated regions. We suggest that proteins that prefer long mRNAs may have relatively short or degenerate binding sites, and that preferences for A or U increase binding in untranslated regions.Fil: Erben, Esteban Daniel. Universidad Nacional de San Martín. Instituto de Investigaciones Biotecnológicas. - Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Biotecnológicas; Argentina. Ruprecht Karls Universitat Heidelberg; AlemaniaFil: Leiss, Kevin. Ruprecht Karls Universitat Heidelberg; AlemaniaFil: Liu, Bin. Ruprecht Karls Universitat Heidelberg; AlemaniaFil: Gil, Diana Inchaustegui. Ruprecht Karls Universitat Heidelberg; AlemaniaFil: Helbig, Claudia. Ruprecht Karls Universitat Heidelberg; AlemaniaFil: Clayton, Christine. Ruprecht Karls Universitat Heidelberg; AlemaniaCambridge University Press2021-02info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/185001Erben, Esteban Daniel; Leiss, Kevin; Liu, Bin; Gil, Diana Inchaustegui; Helbig, Claudia; et al.; Insights into the functions and RNA binding of Trypanosoma brucei ZC3H22, RBP9 and DRBD7; Cambridge University Press; Parasitology; 148; 10; 2-2021; 1186-11950031-1820CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.cambridge.org/core/journals/parasitology/article/insights-into-the-functions-and-rna-binding-of-trypanosoma-brucei-zc3h22-rbp9-and-drbd7/6FE738AFF9CCBC1C9C3783952E4DB306info:eu-repo/semantics/altIdentifier/doi/10.1017/S0031182021000123info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-10T13:11:35Zoai:ri.conicet.gov.ar:11336/185001instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-10 13:11:35.673CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Insights into the functions and RNA binding of Trypanosoma brucei ZC3H22, RBP9 and DRBD7
title Insights into the functions and RNA binding of Trypanosoma brucei ZC3H22, RBP9 and DRBD7
spellingShingle Insights into the functions and RNA binding of Trypanosoma brucei ZC3H22, RBP9 and DRBD7
Erben, Esteban Daniel
MRNA
RNA-BINDING PROTEIN
TRYPANOSOMA BRUCEI
title_short Insights into the functions and RNA binding of Trypanosoma brucei ZC3H22, RBP9 and DRBD7
title_full Insights into the functions and RNA binding of Trypanosoma brucei ZC3H22, RBP9 and DRBD7
title_fullStr Insights into the functions and RNA binding of Trypanosoma brucei ZC3H22, RBP9 and DRBD7
title_full_unstemmed Insights into the functions and RNA binding of Trypanosoma brucei ZC3H22, RBP9 and DRBD7
title_sort Insights into the functions and RNA binding of Trypanosoma brucei ZC3H22, RBP9 and DRBD7
dc.creator.none.fl_str_mv Erben, Esteban Daniel
Leiss, Kevin
Liu, Bin
Gil, Diana Inchaustegui
Helbig, Claudia
Clayton, Christine
author Erben, Esteban Daniel
author_facet Erben, Esteban Daniel
Leiss, Kevin
Liu, Bin
Gil, Diana Inchaustegui
Helbig, Claudia
Clayton, Christine
author_role author
author2 Leiss, Kevin
Liu, Bin
Gil, Diana Inchaustegui
Helbig, Claudia
Clayton, Christine
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv MRNA
RNA-BINDING PROTEIN
TRYPANOSOMA BRUCEI
topic MRNA
RNA-BINDING PROTEIN
TRYPANOSOMA BRUCEI
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Trypanosoma brucei is unusually reliant on mRNA-binding proteins to control mRNA fate, because its protein-coding genes lack individual promoters. We here focus on three trypanosome RNA-binding proteins. ZC3H22 is specific to Tsetse fly forms, RBP9 is preferentially expressed in bloodstream forms; and DRBD7 is constitutively expressed. Depletion of RBP9 or DRBD7 did not affect bloodstream-form trypanosome growth. ZC3H22 depletion from procyclic forms caused cell clumping, decreased expression of genes required for cell growth and proliferation, and increased expression of some epimastigote markers. Apart from decreases in mRNAs encoding enzymes of glucose metabolism, levels of most ZC3H22-bound transcripts were unaffected by ZC3H22 depletion. We compared ZC3H22, RBP9 and DRBD7 RNA binding with that of 16 other RNA-binding proteins. ZC3H22, PUF3 and ERBP1 show a preference for ribosomal protein mRNAs. RBP9 preferentially binds mRNAs that are more abundant in bloodstream forms than in procyclic forms. RBP9, ZC3H5, ZC3H30 and DRBD7 prefer mRNAs with long coding regions; UBP1-associated mRNAs have long 3′-untranslated regions; and RRM1 prefers mRNAs with long 3′or 5′-untranslated regions. We suggest that proteins that prefer long mRNAs may have relatively short or degenerate binding sites, and that preferences for A or U increase binding in untranslated regions.
Fil: Erben, Esteban Daniel. Universidad Nacional de San Martín. Instituto de Investigaciones Biotecnológicas. - Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Biotecnológicas; Argentina. Ruprecht Karls Universitat Heidelberg; Alemania
Fil: Leiss, Kevin. Ruprecht Karls Universitat Heidelberg; Alemania
Fil: Liu, Bin. Ruprecht Karls Universitat Heidelberg; Alemania
Fil: Gil, Diana Inchaustegui. Ruprecht Karls Universitat Heidelberg; Alemania
Fil: Helbig, Claudia. Ruprecht Karls Universitat Heidelberg; Alemania
Fil: Clayton, Christine. Ruprecht Karls Universitat Heidelberg; Alemania
description Trypanosoma brucei is unusually reliant on mRNA-binding proteins to control mRNA fate, because its protein-coding genes lack individual promoters. We here focus on three trypanosome RNA-binding proteins. ZC3H22 is specific to Tsetse fly forms, RBP9 is preferentially expressed in bloodstream forms; and DRBD7 is constitutively expressed. Depletion of RBP9 or DRBD7 did not affect bloodstream-form trypanosome growth. ZC3H22 depletion from procyclic forms caused cell clumping, decreased expression of genes required for cell growth and proliferation, and increased expression of some epimastigote markers. Apart from decreases in mRNAs encoding enzymes of glucose metabolism, levels of most ZC3H22-bound transcripts were unaffected by ZC3H22 depletion. We compared ZC3H22, RBP9 and DRBD7 RNA binding with that of 16 other RNA-binding proteins. ZC3H22, PUF3 and ERBP1 show a preference for ribosomal protein mRNAs. RBP9 preferentially binds mRNAs that are more abundant in bloodstream forms than in procyclic forms. RBP9, ZC3H5, ZC3H30 and DRBD7 prefer mRNAs with long coding regions; UBP1-associated mRNAs have long 3′-untranslated regions; and RRM1 prefers mRNAs with long 3′or 5′-untranslated regions. We suggest that proteins that prefer long mRNAs may have relatively short or degenerate binding sites, and that preferences for A or U increase binding in untranslated regions.
publishDate 2021
dc.date.none.fl_str_mv 2021-02
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/185001
Erben, Esteban Daniel; Leiss, Kevin; Liu, Bin; Gil, Diana Inchaustegui; Helbig, Claudia; et al.; Insights into the functions and RNA binding of Trypanosoma brucei ZC3H22, RBP9 and DRBD7; Cambridge University Press; Parasitology; 148; 10; 2-2021; 1186-1195
0031-1820
CONICET Digital
CONICET
url http://hdl.handle.net/11336/185001
identifier_str_mv Erben, Esteban Daniel; Leiss, Kevin; Liu, Bin; Gil, Diana Inchaustegui; Helbig, Claudia; et al.; Insights into the functions and RNA binding of Trypanosoma brucei ZC3H22, RBP9 and DRBD7; Cambridge University Press; Parasitology; 148; 10; 2-2021; 1186-1195
0031-1820
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://www.cambridge.org/core/journals/parasitology/article/insights-into-the-functions-and-rna-binding-of-trypanosoma-brucei-zc3h22-rbp9-and-drbd7/6FE738AFF9CCBC1C9C3783952E4DB306
info:eu-repo/semantics/altIdentifier/doi/10.1017/S0031182021000123
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Cambridge University Press
publisher.none.fl_str_mv Cambridge University Press
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 12.993085

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