Functional insights from a surface antigen mRNA-bound proteome

Autores
do Nascimento, Larissa Melo; Egler, Franziska; Arnold, Katharina; Papavasiliou, Nina; Clayton, Christine; Erben, Esteban Daniel
Año de publicación
2021
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Trypanosoma brucei is the causative agent of human sleeping sickness. The parasites' Variant Surface Glycoprotein (VSG) enables them to evade adaptive immunity via anti-genic variation. VSG comprises 10% of total cell protein and the high stability of VSG mRNA is essential for trypanosome survival. To determine how VSG mRNA stability is maintained, we used mRNA affinity purification to identify all its associated proteins. CFB2, an unconventional RNA-binding protein with an F-box domain, was specifically enriched with VSG mRNA. We demonstrate that CFB2 is essential for VSG mRNA stabil-ity, describe cis acting elements within the VSG 3'-untranslated region that regulate the interaction, identify trans-acting factors that are present in the VSG messenger ribonu-cleoprotein particle and mechanistically explain how CFB2 stabilizes the mRNA of this key pathogenicity factor. Beyond T. brucei, the mRNP purification approach has the potential to supply detailed biological insight into metabolism of relatively abundant mRNAs in any eukaryote.
Fil: do Nascimento, Larissa Melo. Ruprecht Karls Universitat Heidelberg; Alemania
Fil: Egler, Franziska. Ruprecht Karls Universitat Heidelberg; Alemania
Fil: Arnold, Katharina. Ruprecht Karls Universitat Heidelberg; Alemania
Fil: Papavasiliou, Nina. Ruprecht Karls Universitat Heidelberg; Alemania. Deutsche Krebsforschungszentrum; Alemania
Fil: Clayton, Christine. Ruprecht Karls Universitat Heidelberg; Alemania
Fil: Erben, Esteban Daniel. Universidad Nacional de San Martín. Instituto de Investigaciones Biotecnológicas. - Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Biotecnológicas; Argentina. Ruprecht Karls Universitat Heidelberg; Alemania. Deutsche Krebsforschungszentrum; Alemania
Materia
RNA-BINDING PROTEINS
TRYPANOSOMA BRUCEI
VSG
CFB2
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/185002
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/185002
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Functional insights from a surface antigen mRNA-bound proteomedo Nascimento, Larissa MeloEgler, FranziskaArnold, KatharinaPapavasiliou, NinaClayton, ChristineErben, Esteban DanielRNA-BINDING PROTEINSTRYPANOSOMA BRUCEIVSGCFB2https://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Trypanosoma brucei is the causative agent of human sleeping sickness. The parasites' Variant Surface Glycoprotein (VSG) enables them to evade adaptive immunity via anti-genic variation. VSG comprises 10% of total cell protein and the high stability of VSG mRNA is essential for trypanosome survival. To determine how VSG mRNA stability is maintained, we used mRNA affinity purification to identify all its associated proteins. CFB2, an unconventional RNA-binding protein with an F-box domain, was specifically enriched with VSG mRNA. We demonstrate that CFB2 is essential for VSG mRNA stabil-ity, describe cis acting elements within the VSG 3'-untranslated region that regulate the interaction, identify trans-acting factors that are present in the VSG messenger ribonu-cleoprotein particle and mechanistically explain how CFB2 stabilizes the mRNA of this key pathogenicity factor. Beyond T. brucei, the mRNP purification approach has the potential to supply detailed biological insight into metabolism of relatively abundant mRNAs in any eukaryote.Fil: do Nascimento, Larissa Melo. Ruprecht Karls Universitat Heidelberg; AlemaniaFil: Egler, Franziska. Ruprecht Karls Universitat Heidelberg; AlemaniaFil: Arnold, Katharina. Ruprecht Karls Universitat Heidelberg; AlemaniaFil: Papavasiliou, Nina. Ruprecht Karls Universitat Heidelberg; Alemania. Deutsche Krebsforschungszentrum; AlemaniaFil: Clayton, Christine. Ruprecht Karls Universitat Heidelberg; AlemaniaFil: Erben, Esteban Daniel. Universidad Nacional de San Martín. Instituto de Investigaciones Biotecnológicas. - Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Biotecnológicas; Argentina. Ruprecht Karls Universitat Heidelberg; Alemania. Deutsche Krebsforschungszentrum; AlemaniaeLife Sciences Publications Ltd2021-03info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/185002do Nascimento, Larissa Melo; Egler, Franziska; Arnold, Katharina; Papavasiliou, Nina; Clayton, Christine; et al.; Functional insights from a surface antigen mRNA-bound proteome; eLife Sciences Publications Ltd; eLife; 10; 3-2021; 1-262050-084XCONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://elifesciences.org/articles/68136info:eu-repo/semantics/altIdentifier/doi/10.7554/eLife.68136info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-15T15:23:26Zoai:ri.conicet.gov.ar:11336/185002instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-15 15:23:26.815CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Functional insights from a surface antigen mRNA-bound proteome
title Functional insights from a surface antigen mRNA-bound proteome
spellingShingle Functional insights from a surface antigen mRNA-bound proteome
do Nascimento, Larissa Melo
RNA-BINDING PROTEINS
TRYPANOSOMA BRUCEI
VSG
CFB2
title_short Functional insights from a surface antigen mRNA-bound proteome
title_full Functional insights from a surface antigen mRNA-bound proteome
title_fullStr Functional insights from a surface antigen mRNA-bound proteome
title_full_unstemmed Functional insights from a surface antigen mRNA-bound proteome
title_sort Functional insights from a surface antigen mRNA-bound proteome
dc.creator.none.fl_str_mv do Nascimento, Larissa Melo
Egler, Franziska
Arnold, Katharina
Papavasiliou, Nina
Clayton, Christine
Erben, Esteban Daniel
author do Nascimento, Larissa Melo
author_facet do Nascimento, Larissa Melo
Egler, Franziska
Arnold, Katharina
Papavasiliou, Nina
Clayton, Christine
Erben, Esteban Daniel
author_role author
author2 Egler, Franziska
Arnold, Katharina
Papavasiliou, Nina
Clayton, Christine
Erben, Esteban Daniel
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv RNA-BINDING PROTEINS
TRYPANOSOMA BRUCEI
VSG
CFB2
topic RNA-BINDING PROTEINS
TRYPANOSOMA BRUCEI
VSG
CFB2
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Trypanosoma brucei is the causative agent of human sleeping sickness. The parasites' Variant Surface Glycoprotein (VSG) enables them to evade adaptive immunity via anti-genic variation. VSG comprises 10% of total cell protein and the high stability of VSG mRNA is essential for trypanosome survival. To determine how VSG mRNA stability is maintained, we used mRNA affinity purification to identify all its associated proteins. CFB2, an unconventional RNA-binding protein with an F-box domain, was specifically enriched with VSG mRNA. We demonstrate that CFB2 is essential for VSG mRNA stabil-ity, describe cis acting elements within the VSG 3'-untranslated region that regulate the interaction, identify trans-acting factors that are present in the VSG messenger ribonu-cleoprotein particle and mechanistically explain how CFB2 stabilizes the mRNA of this key pathogenicity factor. Beyond T. brucei, the mRNP purification approach has the potential to supply detailed biological insight into metabolism of relatively abundant mRNAs in any eukaryote.
Fil: do Nascimento, Larissa Melo. Ruprecht Karls Universitat Heidelberg; Alemania
Fil: Egler, Franziska. Ruprecht Karls Universitat Heidelberg; Alemania
Fil: Arnold, Katharina. Ruprecht Karls Universitat Heidelberg; Alemania
Fil: Papavasiliou, Nina. Ruprecht Karls Universitat Heidelberg; Alemania. Deutsche Krebsforschungszentrum; Alemania
Fil: Clayton, Christine. Ruprecht Karls Universitat Heidelberg; Alemania
Fil: Erben, Esteban Daniel. Universidad Nacional de San Martín. Instituto de Investigaciones Biotecnológicas. - Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Biotecnológicas; Argentina. Ruprecht Karls Universitat Heidelberg; Alemania. Deutsche Krebsforschungszentrum; Alemania
description Trypanosoma brucei is the causative agent of human sleeping sickness. The parasites' Variant Surface Glycoprotein (VSG) enables them to evade adaptive immunity via anti-genic variation. VSG comprises 10% of total cell protein and the high stability of VSG mRNA is essential for trypanosome survival. To determine how VSG mRNA stability is maintained, we used mRNA affinity purification to identify all its associated proteins. CFB2, an unconventional RNA-binding protein with an F-box domain, was specifically enriched with VSG mRNA. We demonstrate that CFB2 is essential for VSG mRNA stabil-ity, describe cis acting elements within the VSG 3'-untranslated region that regulate the interaction, identify trans-acting factors that are present in the VSG messenger ribonu-cleoprotein particle and mechanistically explain how CFB2 stabilizes the mRNA of this key pathogenicity factor. Beyond T. brucei, the mRNP purification approach has the potential to supply detailed biological insight into metabolism of relatively abundant mRNAs in any eukaryote.
publishDate 2021
dc.date.none.fl_str_mv 2021-03
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/185002
do Nascimento, Larissa Melo; Egler, Franziska; Arnold, Katharina; Papavasiliou, Nina; Clayton, Christine; et al.; Functional insights from a surface antigen mRNA-bound proteome; eLife Sciences Publications Ltd; eLife; 10; 3-2021; 1-26
2050-084X
CONICET Digital
CONICET
url http://hdl.handle.net/11336/185002
identifier_str_mv do Nascimento, Larissa Melo; Egler, Franziska; Arnold, Katharina; Papavasiliou, Nina; Clayton, Christine; et al.; Functional insights from a surface antigen mRNA-bound proteome; eLife Sciences Publications Ltd; eLife; 10; 3-2021; 1-26
2050-084X
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://elifesciences.org/articles/68136
info:eu-repo/semantics/altIdentifier/doi/10.7554/eLife.68136
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv eLife Sciences Publications Ltd
publisher.none.fl_str_mv eLife Sciences Publications Ltd
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1846083381487468544
score 13.22299

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